scholarly journals Genomics Analyses of GIV and GVI Noroviruses Reveal the Distinct Clustering of Human and Animal Viruses

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 204 ◽  
Author(s):  
Lauren Ford-Siltz ◽  
Lisa Mullis ◽  
Yasser Sanad ◽  
Kentaro Tohma ◽  
Cara Lepore ◽  
...  
Keyword(s):  
United States ◽  
Acute Gastroenteritis ◽  
Major Capsid Protein ◽  
Mammalian Species ◽  
The United States ◽  
Structural Proteins ◽  
Full Genome ◽  
Complete Genomes ◽  
Animal Strains ◽  
Canine Norovirus

Noroviruses are highly diverse viruses that are the major viral cause of acute gastroenteritis in humans. Although these viruses can infect multiple mammalian species, their potential for zoonosis is not well understood, especially within Genogroup IV (GIV), which contains viruses that infect humans, canines, and felines. The study of GIV viruses has been, in part, hindered by the limited number of complete genomes. Here, we developed a full-genome amplicon-based platform that facilitated the sequencing of canine noroviruses circulating in the United States. Eight novel nearly full-length canine norovirus genomes and two nearly complete VP1 sequences, including four GIV.2, three GVI.1, and three GVI.2 viruses, were successfully obtained. Only animal strains exhibited GVI/GIV chimeric viruses, demonstrating restrictions in norovirus recombination. Using genomic, phylogenetic, and structural analyses, we show that differences within the major capsid protein and the non-structural proteins of GIV and GVI noroviruses could potentially limit cross-species transmission between humans, canines, and felines.

scholarly journals Sequences of Seven Complete Genomes of Human Parvovirus B19

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Yuhuan Qiu ◽  
Zehui Zhao ◽  
Jianming Qiu
Keyword(s):  
United States ◽  
Human Plasma ◽  
Parvovirus B19 ◽  
The United States ◽  
Human Parvovirus B19 ◽  
Genome Sequences ◽  
Complete Genomes ◽  
Inverted Terminal Repeats ◽  
Terminal Repeats

We are reporting the sequences of seven complete genomes of parvovirus B19, which were extracted from human plasma specimens collected in the United States. The seven B19 genome sequences, which are 5,596 nucleotides long and include the inverted terminal repeats (ITRs), share an identity of 96.73%.

scholarly journals Complete Genome Sequence of a Human Norovirus Strain from the United States Classified as Genotype GII.P6_GII.6

2018 ◽  
Vol 6 (22) ◽  
Author(s):  
Haifeng Chen ◽  
Shiliang Wang ◽  
Weimin Wang
Keyword(s):  
United States ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
The United States ◽  
Virus Genome ◽  
Full Genome Sequence ◽  
Full Genome ◽  
Virus Genotype

ABSTRACT We report here the complete genome sequence of a GII.6 norovirus strain detected in a clinical fecal specimen from the United States. The virus genome has a length of 7,547 bp and a GC content of 50.1%. Complete norovirus genotyping of the full-genome sequence identified the virus genotype as GII.P6_GII.6.

scholarly journals Molecular and Epidemiologic Trends of Caliciviruses Associated with Outbreaks of Acute Gastroenteritis in the United States, 2000–2004

2006 ◽  
Vol 193 (3) ◽  
pp. 413-421 ◽  
Author(s):  
Lenee H. Blanton ◽  
Susan M. Adams ◽  
R. Suzanne Beard ◽  
Gang Wei ◽  
Sandra N. Bulens ◽  
...  
Keyword(s):  
United States ◽  
Acute Gastroenteritis ◽  
The United States

scholarly journals 1099. Antibiotic Prescriptions for Acute Gastroenteritis during Office and Emergency Department Visits–United States, 2006–2015

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S330-S330
Author(s):  
Jennifer P Collins ◽  
Louise Francois Watkins ◽  
Laura M King ◽  
Monina Bartoces ◽  
Katherine Fleming-Dutra ◽  
...  
Keyword(s):  
United States ◽  
Emergency Department ◽  
Medical Care ◽  
Acute Gastroenteritis ◽  
Antibiotic Use ◽  
The United States ◽  
Emergency Department Visits ◽  
Antibiotic Prescribing ◽  
Care Survey ◽  
Ambulatory Medical Care

Abstract Background Acute gastroenteritis (AGE) is a major cause of office and emergency department (ED) visits in the United States. Most patients can be managed with supportive care alone, although some require antibiotics. Limiting unnecessary antibiotic use can minimize side effects and the development of resistance. We used national data to assess antibiotic prescribing for AGE to target areas for stewardship efforts. Methods We used the 2006–2015 National Hospital Ambulatory Medical Care Survey of EDs and National Ambulatory Medical Care Survey to describe antibiotic prescribing for AGE. An AGE visit was defined as one with a new problem (<3 months) as the main visit indication and an ICD-9 code for bacterial or viral gastrointestinal infection or AGE symptoms (nausea, vomiting, and/or diarrhea). We excluded visits with ICD-9 codes for Clostridium difficile or an infection usually requiring antibiotics (e.g., pneumonia). We calculated national annual percentage estimates based on weights of sampled visits and used an α level of 0.01, recommended for these data. Results Of the 12,191 sampled AGE visits, 13% (99% CI: 11–15%) resulted in antibiotic prescriptions, equating to an estimated 1.3 million AGE visits with antibiotic prescriptions annually. Antibiotics were more likely to be prescribed in office AGE visits (16%, 99% CI: 12–20%) compared with ED AGE visits (11%, 99% CI: 9–12%; P < 0.01). Among AGE visits with antibiotic prescriptions, the most frequently prescribed were fluoroquinolones (29%, 99% CI: 21–36%), metronidazole (18%, 99% CI: 13–24%), and penicillins (18%, 99% CI: 11–24%). Antibiotics were prescribed for 25% (99% CI: 8–42%) of visits for bacterial AGE, 16% (99% CI: 12–21%) for diarrhea without nausea or vomiting, and 11% (99% CI: 8–15%) for nausea, vomiting, or both without diarrhea. Among AGE visits with fever (T ≥ 100.9oF) at the visit, 21% (99% CI: 11–31%) resulted in antibiotic prescriptions. Conclusion Patients treated for AGE in office settings were significantly more likely to receive prescriptions for antibiotics compared with those seen in an ED, despite likely lower acuity. Antibiotic prescribing was also high for visits for nausea or vomiting, conditions that usually do not require antibiotics. Antimicrobial stewardship for AGE is needed, especially in office settings. Disclosures All authors: No reported disclosures.

scholarly journals Near-Complete Genome Sequences of Several New Norovirus Genogroup II Genotypes

2018 ◽  
Vol 6 (6) ◽  
pp. e00007-18 ◽  
Author(s):  
Preeti Chhabra ◽  
Kshama Aswath ◽  
Nikail Collins ◽  
Tahmeed Ahmed ◽  
Maribel Paredes Olórtegui ◽  
...  
Keyword(s):  
United States ◽  
Acute Gastroenteritis ◽  
Complete Genome ◽  
The United States ◽  
Genome Sequences ◽  
Stool Samples ◽  
Genotype Ii

ABSTRACTWe report here the near-complete genome sequences of 13 norovirus strains detected in stool samples from patients with acute gastroenteritis from Bangladesh, Ecuador, Guatemala, Peru, Nicaragua, and the United States that are classified into one existing (genotype II.22 [GII.22]), 3 novel (GII.23, GII.24 and GII.25), and 3 tentative novel (GII.NA1, GII.NA2, and GII.NA3) genotypes.

scholarly journals The Etiology of Severe Acute Gastroenteritis Among Adults Visiting Emergency Departments in the United States

2012 ◽  
Vol 205 (9) ◽  
pp. 1374-1381 ◽  
Author(s):  
Joseph S. Bresee ◽  
Ruthanne Marcus ◽  
Richard A. Venezia ◽  
William E. Keene ◽  
Dale Morse ◽  
...  
Keyword(s):  
United States ◽  
Acute Gastroenteritis ◽  
Emergency Departments ◽  
The United States

scholarly journals Genetic and Epidemiologic Trends of Norovirus Outbreaks in the United States from 2013 to 2016 Demonstrated Emergence of Novel GII.4 Recombinant Viruses

2017 ◽  
Vol 55 (7) ◽  
pp. 2208-2221 ◽  
Author(s):  
Jennifer L. Cannon ◽  
Leslie Barclay ◽  
Nikail R. Collins ◽  
Mary E. Wikswo ◽  
Christina J. Castro ◽  
...  
Keyword(s):  
United States ◽  
Acute Gastroenteritis ◽  
The United States ◽  
Rna Polymerases ◽  
Common Ancestry ◽  
Molecular Surveillance ◽  
Recombinant Viruses

ABSTRACT Noroviruses are the most frequent cause of epidemic acute gastroenteritis in the United States. Between September 2013 and August 2016, 2,715 genotyped norovirus outbreaks were submitted to CaliciNet. GII.4 Sydney viruses caused 58% of the outbreaks during these years. A GII.4 Sydney virus with a novel GII.P16 polymerase emerged in November 2015, causing 60% of all GII.4 outbreaks in the 2015-2016 season. Several genotypes detected were associated with more than one polymerase type, including GI.3, GII.2, GII.3, GII.4 Sydney, GII.13, and GII.17, four of which harbored GII.P16 polymerases. GII.P16 polymerase sequences associated with GII.2 and GII.4 Sydney viruses were nearly identical, suggesting common ancestry. Other common genotypes, each causing 5 to 17% of outbreaks in a season, included GI.3, GI.5, GII.2, GII.3, GII.6, GII.13, and GII.17 Kawasaki 308. Acquisition of alternative RNA polymerases by recombination is an important mechanism for norovirus evolution and a phenomenon that was shown to occur more frequently than previously recognized in the United States. Continued molecular surveillance of noroviruses, including typing of both polymerase and capsid genes, is important for monitoring emerging strains in our continued efforts to reduce the overall burden of norovirus disease.

scholarly journals Gastroenteritis in adults

2018 ◽  
Vol 5 (11) ◽  
pp. 4959
Author(s):  
Burair Al Jassas ◽  
Marwan Khayat ◽  
Hussin Alzahrani ◽  
Aghareed Asali ◽  
Salem Alsohaimi ◽  
...  
Keyword(s):  
United States ◽  
Abdominal Pain ◽  
Acute Gastroenteritis ◽  
Viral Infections ◽  
Diarrheal Disease ◽  
The United States ◽  
Viral Gastroenteritis ◽  
Search Terms ◽  
Bacterial Gastroenteritis ◽  
Comprehensive Search

Gastroenteritis is the inflammation of intestines and stomach which presents with vomiting, fever, abdominal pain and diarrhea. It could be persistent, acute, or chronic, and can also be classified as infectious or non-infectious. Despite improvement in management, the mortality can reach up to 17,000. In this study, our aim was to understand the various etiologies that cause gastroenteritis in adults, and also discuss methods of management. We conducted this review using a comprehensive search of MEDLINE, PubMed and EMBASE from January 1994 to March 2017. The following search terms were used: acute gastroenteritis, diarrheal disease, viral gastroenteritis, bacterial gastroenteritis, diagnoses of gastroenteritis. Each year, more than 350 million cases of acute gastroenteritis occur in the United States only. The largest portion of gastroenteritis cases is due to viral infections. Therefore, the empiric use of antibiotics is usually not recommended. However, in selected patients, empiric antibiotics therapy is indicated and is associated with significant improvement and decrease in mortality. The primary goal of management of gastroenteritis is treating dehydration.

scholarly journals Assessing the risks to United States and Canadian mammals caused by climate change using a trait-mediated model

2019 ◽  
Author(s):  
Christy M McCain
Keyword(s):  
Climate Change ◽  
United States ◽  
Large Scale ◽  
Human Impacts ◽  
Mammalian Species ◽  
Species Traits ◽  
The United States ◽  
Mammal Species ◽  
Climate Change Risk ◽  
Very High

Abstract A set of 182 populations of 76 mammal species in the United States and Canada, examined in natural conditions with minimized disturbances or management effects, shows that responses to climate change include negative responses, such as elevational range contractions, upward shifts and decreases in abundance, positive responses, such as range expansions, and no detectable responses. Responses vary among and within mammal species but many are correlated with species traits, particularly the responses linked to high extinction risks (= climate change risk: decreases in population sizes, range contractions, local extirpations). The traits showing the strongest links to differential responses to climate change are 1) body size—large mammals respond more often and most negatively to climate change, 2) activity times—few mammals with flexible active times respond to climate change, and 3) spatial distribution—high-latitude and high-elevation mammals responded more often to climate change. Using these traits and two approaches to trait weighting, I modeled the relative climate change risk for all 328 terrestrial, nonvolant mammal species in the United States and Canada across 10 levels of risk (low = 1–2, moderate = 3–4, moderate-high = 5–6, high = 7–8, very high = 9–10). The models predicted that 15% of these mammalian species are in the high- and very high-risk categories, including species from most orders. Many mammal populations and species listed as of conservation concern due to other human impacts by national or international agencies are also predicted by my models to be in the higher categories of climate change risk. My intention for these models is to clarify for managers and researchers which, where, and how mammals are responding to climate change relatively independent of other anthropogenic stressors (e.g., large-scale habitat change, overhunting) and to provide a preliminary assessment of species most in need of careful monitoring for climate change impacts.

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