scholarly journals Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus

Vaccines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1265
Author(s):  
Hyeon-Jeong Go ◽  
Byung-Joo Park ◽  
Hee-Seop Ahn ◽  
Dong-Hwi Kim ◽  
Da-Yoon Kim ◽  
...  
Keyword(s):  
Expression System ◽  
Vaccine Dose ◽  
Baculovirus Expression System ◽  
Hepatitis E ◽  
Positive Control ◽  
Negative Control ◽  
Fecal Shedding ◽  
Viral Shedding ◽  
Baculovirus Expression ◽  
Virus Like Particle

In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive control, 100 μg VLP-, and 200 μg VLP-vaccinated groups for 10 weeks. The pigs in either of the vaccinated groups were administered the corresponding first and booster doses on weeks 0 and 2. At week 4, the positive control and two vaccinated groups were challenged with 106 HEV-3 genomic equivalent copies; viremia and fecal shedding of the virus were identified in pigs in the positive control and 100 μg VLP-vaccinated pigs showed transient viremia and fecal viral shedding. However, no viruses were detected in the serum or fecal samples of the 200 μg VLP-vaccinated pigs. The 100 and 200 μg VLP-vaccinated pigs had significantly higher (p < 0.01) anti-HEV antibodies than the negative control pigs from weeks 6–10 with normal levels of liver enzymes. The 200 μg VLP-vaccinated pigs showed statistically less liver tissue fibrosis (p < 0.05) than that of the positive control pigs. Thus, the novel baculovirus expression system-generated VLP vaccine dose-dependently protects against HEV-3 challenge and may be useful in other animal species, including humans.

scholarly journals Cloning and expression of hepatitis E virus ORF2 as an immunogen protein in baculovirus expression system

2018 ◽  
Vol 5 (1) ◽  
pp. 23-26
Author(s):  
SA Sadeghi ◽  
M Shahanaghi ◽  
MR Aghasadeghi ◽  
F Motevalli ◽  
MR Amiran ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Hepatitis E ◽  
Cloning And Expression ◽  
Baculovirus Expression

scholarly journals RG203KR mutations in SARS-CoV-2 Nucleocapsid: Assessing the impact using Virus-like particle model system

2022 ◽  
Author(s):  
Harsha Raheja ◽  
Soma Das ◽  
Anindita Banerjee ◽  
Dikshaya P ◽  
Deepika C ◽  
...  
Keyword(s):  
Expression System ◽  
Recombinant Baculovirus ◽  
Baculovirus Expression System ◽  
Structural Proteins ◽  
Particle Model ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Baculovirus Expression ◽  
Virus Like Particle ◽  
The Impact

The emergence and evolution of SARS-CoV-2 is characterized by the occurrence of diverse sets of mutations that affect virus characteristics, including transmissibility and antigenicity. Recent studies have focused mostly on Spike protein mutations; however, SARS-CoV-2 variants of interest (VoI) or concern (VoC) contain significant mutations in the nucleocapsid protein as well. To study the relevance of the mutations at the virion level, recombinant baculovirus expression system based VLPs were generated for the prototype Wuhan sequence along with Spike mutants like D614G, G1124V and the significant RG203KR mutation in Nucleocapsid. All the four structural proteins assembled in a particle wherein the morphology and size of the particle confirmed by TEM closely resembles the native virion. The VLP harbouring RG203KR mutations in nucleocapsid exhibited augmentation of humoral immune responses and enhanced neutralization by the immunized mice sera. Results demonstrate a non-infectious platform to quickly assess the implication of mutations in structural proteins of the emerging variant.

scholarly journals Expression of functional G protein beta gamma dimers of defined subunit composition using a baculovirus expression system.

1992 ◽  
Vol 267 (19) ◽  
pp. 13123-13126 ◽  
Author(s):  
S.G. Graber ◽  
R.A. Figler ◽  
V.K. Kalman-Maltese ◽  
J.D. Robishaw ◽  
J.C. Garrison
Keyword(s):  
G Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Subunit Composition ◽  
Baculovirus Expression

scholarly journals Production, processing and partial purification of functional G protein βγ subunits in baculovirus-infected insect cells

1992 ◽  
Vol 286 (3) ◽  
pp. 677-680 ◽  
Author(s):  
J D Robishaw ◽  
V K Kalman ◽  
K L Proulx
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Insect Cells ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Partial Purification ◽  
Baculovirus Expression ◽  
Gamma Subunits ◽  
Infected Insect ◽  
Beta 2

As a result of the inability to resolve the heterogeneous mixture of G protein beta gamma subunits present in tissues, it has not been possible to compare different beta gamma subunits of the G proteins in terms of their proposed roles in receptor-effector coupling. This study was undertaken to establish the utility of the baculovirus expression system in producing homogeneous beta gamma subunits of defined composition for the comparative analysis of these subunits in reconstitution systems. In this study we report the expression, and appropriate post-translational processing, of recombinant beta 2, gamma 2 and gamma 3 subunits. In addition, we show that the recombinant beta gamma subunits can be readily purified, and can functionally interact with the alpha subunits of the G proteins.

scholarly journals Efficient production of recombinant PP2A at a low temperature using a baculovirus expression system

2016 ◽  
Vol 11 ◽  
pp. 86-89 ◽  
Author(s):  
Tsuyoshi Ikehara ◽  
Shihoko Nakashima ◽  
Junichi Nakashima ◽  
Tsubasa Kinoshita ◽  
Takeshi Yasumoto
Keyword(s):  
Low Temperature ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Efficient Production ◽  
Baculovirus Expression
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