Investigation of the antibacterial activity of the nanostructured system of copper sulfate in Na-carboxymethyl cellulose on the example of an indigenous strain of the gram-negative bacterium Esherichia coli

Synthesis, Characterizations, and Biological Effects Study of Some Quinoline Family

Emerging Research on Bioinspired Materials Engineering - Advances in Chemical and Materials Engineering ◽

10.4018/978-1-4666-9811-6.ch006 ◽

2016 ◽

pp. 160-196

Author(s):

Messai Amel

Keyword(s):

Antibacterial Activity ◽

Single Crystal ◽

Biological Activities ◽

Biological Effects ◽

Diffusion Method ◽

Quinoline Derivatives ◽

Single Crystal Diffraction ◽

Disk Diffusion Method ◽

New Approach ◽

Gram Negative

The objective of this work is the synthesis of new quinoline molecules which could have some biological activities. This chapter reported a new approach to the synthesis of some quinoline derivatives. The Baylis-Hillman reaction on 2-methoxy-3-formyl quinoléines derivatives have applied in order to obtain Baylis-Hillman adducts. The products are characterized by FTIR, NMR and X-ray single crystal diffraction .Also, a study of the antibacterial activity of the 3-(2-chloro quinoline)-3-hydroxy-2 methylene propanonitrile products synthesized have been explored. This assessment is made by using the disk diffusion method. The results showed that the 3-(2'-chloroquinoline)-3-hydroxy-2-methylenepropanonitril derivatives present a good antibacterial effectiveness against the strains tested Gram-positive and no antibacterial potency was observed against the stains Gram-negative used in the test.

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Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

Natural Product Communications ◽

10.1177/1934578x1200700530 ◽

2012 ◽

Vol 7 (5) ◽

pp. 1934578X1200700 ◽

Cited By ~ 2

Author(s):

Alexis Peña ◽

Luis Rojas ◽

Rosa Aparicio ◽

Libia Alarcón ◽

José Gregorio Baptista ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

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ISOLASI DAN UJI ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN SPONS Phyllospongia lamellose SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽

10.35799/pha.8.2019.29260 ◽

2019 ◽

Vol 8 (1) ◽

pp. 252

Author(s):

Sela S Lempoy ◽

Widya A Lolo ◽

Paulina V. Y. Yamlean

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bioactive Compounds ◽

Pathogenic Bacteria ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Isolation And Purification ◽

Esherichia Coli ◽

Identification Stage

ABSTRACT Sponges are one source of producing bioactive components from the sea. Bacteria Symbiosis with sponges are thought to have the potential to produce bioactive compounds that have been isolated from sponges. One of the potential of bioactive compounds which have been found and developed from sponges was antibacterial. The aim of this study was to determine the antibacterial activity of bacteria associated with Phyllospongia lamellose sponges against pathogenic bacteria namely Staphylococcus aureus and Esherichia coli and then proceed with identification of biochemistry in isolates which showed the greatest inhibitory activity. Three isolates of sponges symbiont bacteria were obtained through the isolation and purification stage which were then followed by testing of antibacterial activity with paper disk diffusion method. The antibacterial test results showed the diameter of the inhibitory zone against Staphylococcus aureus, were: SL1(8.67 mm), SL2 (9.33 mm) and SL3(9.00 mm) categorized as medium. Whereas the inhibiotion zone shown on Esherichia coli bacteria is also classified as medium, were: SL1(9.67 mm), SL2 (9.00 mm) and SL3 (9.33 mm). The three isolates continued to the identification stage biochemically. Each isolation was assumed as follows: Desulfotomaculum (SL1), Brochothrix (SL2) and Sulfidobacillus (SL3). Keyword :Phyllospongia lamellose, isolation, biochemically identification ABSTRAK Spons merupakan salah satu sumber penghasil komponen bioaktif yang berasal dari laut. Bakteri yang bersimbiosis dengan spons diduga memiliki potensi dalam menghasilkan senyawa-senyawa bioaktif yang telah diisolasi dari spons. Potensi senyawa bioaktif yang telah ditemukan dan dikembangkan dari spons salah satunya ialah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari bakteri yang berasosiasi dengan spons Phyllospongia lamellose terhadap bakteri patogen yakni Staphylococcus aureus dan Escherichia coli dan kemudian dilanjutkan dengan identifikasi secara biokimia pada isolat yang menunjukkan daya hambat terbesar. Diperoleh 3 isolat bakteri simbion spons melalui tahap isolasi dan purifikasi yang kemudian dilanjutkan dengan pengujian aktivitas antibakteri melalui metode difusi kertas cakram. Hasil uji antibakteri menunjukkan diameter zona hambat terhadap bakteri Staphylococcus aureus yaitu: SL1(8.67 mm), SL2(9.33 mm) dan SL3 (9.00 mm) termasuk dalam kategori sedang. Sedangkan zona hambat yang ditunjukkan terhadap bakteri Escherichia coli juga tergolong kategori sedang yaitu: SL1(9.67 mm), SL2(9.00 mm) dan SL3 (9.33 mm). Ketiga isolat dilanjutkan ke tahap identifikasi secara biokimia. Masing-masing isolat diduga sebagai berikut: Desulfotomaculum (SL1), Brochothrix (SL2) dan Sulfidobacillus (SL3). Kata kunci :Phyllospongia lamellose, isolasi, identifikasi biokimia.

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In vitro Antibacterial Activity of 7-Substituted-6-Fluoroquinolone and 7-Substituted-6,8-Difluoroquinolone Derivatives

Chemotherapy ◽

10.1159/000456533 ◽

2017 ◽

Vol 62 (3) ◽

pp. 194-198 ◽

Cited By ~ 3

Author(s):

Socorro Leyva-Ramos ◽

Denisse de Loera ◽

Jaime Cardoso-Ortiz

Keyword(s):

Antibacterial Activity ◽

Antimicrobial Agents ◽

New Drugs ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Topoisomerase Iv ◽

Gram Positive ◽

Disk Diffusion Method ◽

Gram Negative

Background: Fluoroquinolones are widely prescribed synthetic antimicrobial agents. Quinolones act by converting their targets, gyrase and topoisomerase IV, into toxic enzymes that fragment the bacterial chromosome; the irreversible DNA damage eventually causes the killing of bacteria. Thorough knowledge of the structure-activity relationship of quinolones is essential for the development of new drugs with improved activity against resistant strains. Methods: The compounds were screened for their antibacterial activity against 4 representing strains using the Kirby-Bauer disk diffusion method. Minimal inhibitory concentration (MIC) was determined by measuring the diameter of the inhibition zone using concentrations between 250 and 0.004 μg/mL. Results: MIC of derivatives 2, 3, and 4 showed potent antimicrobial activity against gram-positive and gram-negative bacteria. The effective concentrations were 0.860 μg/mL or lower. MIC for compounds 5-11 were between 120 and 515 μg/mL against Escherichia coli and Staphylococcus aureus, and substituted hydrazinoquinolones 7-10 showed poor antibacterial activity against gram-positive and gram-negative bacteria compared with other quinolones. Conclusion: Compounds obtained by modifications on C-7 of norfloxacin with the acetylated piperazinyl, halogen atoms, and substituted hydrazinyl showed good in vitro activity - some even better than the original compound.

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Comparison of Antimicrobial Activity ofAllium sativumCloves from China and Taşköprü, Turkey

Advances in Pharmacological Sciences ◽

10.1155/2018/9302840 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Ali Yetgin ◽

Kerem Canlı ◽

Ergin Murat Altuner

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria

In this study, antimicrobial activities of two different samples ofAllium sativumL. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species ofBacillus,Enterobacter,Enterococcus,Escherichia,Klebsiella,Listeria,Pseudomonas,Salmonella, andStaphylococcuswere used for testing antibacterial activity. In addition, antifungal activity againstCandida albicanswas also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.

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Screening of Callicarpa arborea and Hemigraphis alternata for antibacterial activity

Science Vision ◽

10.33493/scivis.20.02.02 ◽

2020 ◽

Vol 20 (2) ◽

pp. 72-77

Author(s):

Kholhring Lalchhandama ◽

J. H. Zomuanpuia ◽

Lai thangliani ◽

R. L. Malsawmdawngkimi ◽

Hulianthanga ◽

...

Keyword(s):

Antibacterial Activity ◽

Micrococcus Luteus ◽

Antimicrobial Property ◽

Stem Bark ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Good Source ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria

Callicarpa arborea and Hemigraphis alternata are two medicinal plants claimed to have antimicrobial property in Indian traditional medicine. The methanol extracts of the stem bark of C. arborea and the leaves of H. alternata were prepared and tested for antibacterial activity using disk diffusion method. Four Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhimurium; and two Gram-positive bacteria such as Micrococcus luteus and Bacillus subtilis were used. C. arborea extract was effective against all the bacteria tested, while H. alternata did not show any inhibitory activity. These findings suggest that C. arborea is a good source of antibacterial compound.

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Antimicrobial activity of leaf and flower extracts of Lippia Nodiflora L. (Verbenacea)

Journal of Plant Protection Research ◽

10.2478/v10045-012-0065-9 ◽

2012 ◽

Vol 52 (4) ◽

pp. 401-403 ◽

Cited By ~ 4

Author(s):

Zahra Zare ◽

Ahmed Majd ◽

Taher Nejad Sattari ◽

Alireza Iranbakhsh ◽

Sedigue Mehrabian

Keyword(s):

Antimicrobial Activity ◽

Micrococcus Luteus ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Methanolic Extracts ◽

Esherichia Coli

Abstract Antimicrobial activities of the methanolic extracts from the leaves and flowers of Lippia nodiflora L. (Verbenaceae), were studied by the disk diffusion method. The extracts showed antimicrobial impact on bacteria such as Bacillus subtilis, B. cereus, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, K. oxytoca and Esherichia coli as well as fungi such Aspergillus niger and Candida albicanse. The results showed that increasing concentrations of extracts increased the antimicrobial activities in all of the microorganisms. Bacteria were more sensitive than fungi, and gram positive bacteria were more sensitive than gram negative ones.

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In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

International Journal of Pharmaceutical and Phytopharmacological Research ◽

10.24896/eijppr.2016641 ◽

2016 ◽

Vol 6 (4) ◽

pp. 68

Author(s):

Sushma Vashisht ◽

Manish Pal Singh ◽

Viney Chawla

Keyword(s):

Antibacterial Activity ◽

Methanolic Extract ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Disc Diffusion ◽

Gram Positive ◽

Gram Negative ◽

Shorea Robusta ◽

Dose Dependent ◽

Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

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Antibacterial activity of magnesium oxide nanoparticles prepared by Calcination method

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.3.25 ◽

2019 ◽

Vol 10 (03) ◽

Author(s):

Elaf Ayad Kadhem ◽

Miaad Hamzah Zghair ◽

Sarah , Hussam H. Tizkam, Shoeb Alahmad Salih Mahdi ◽

Hussam H. Tizkam ◽

Shoeb Alahmad

Keyword(s):

Antibacterial Activity ◽

Magnesium Oxide ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Magnesium Oxide Nanoparticles ◽

Agar Disc

magnesium oxide nanoparticles (MgO NPs) were prepared by simple wet chemical method using different calcination temperatures. The prepared NPs were characterized by Electrostatic Discharge (ESD), Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD). It demonstrates sharp intensive peak with the increase of crystallinty and increase of the size with varying morphologies with respect to increase of calcination temperature. Antibacterial studies were done on gram negative bacteria (E.coli) and gram positive bacteria (S.aureus) by agar disc diffusion method. The zones of inhibitions were found larger for gram positive bacteria than gram negative bacteria, this mean, antibacterial MgO NPs activity more active on gram positive bacteria than gram negative bacteria because of the structural differences. It was found that antibacterial activity of MgO NPs was found it has directly proportional with their concentration.

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Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

RENDICONTI LINCEI ◽

10.1007/s12210-021-00991-5 ◽

2021 ◽

Author(s):

Filippo Fratini ◽

Margherita Giusti ◽

Simone Mancini ◽

Francesca Pisseri ◽

Basma Najar ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Diffusion Method ◽

Minimal Bactericidal Concentration ◽

Coagulase Negative Staphylococci ◽

Disk Diffusion Method ◽

In Vitro Antibacterial Activity ◽

High Concentrations ◽

Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

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