Faculty Opinions recommendation of Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium.

2012 ◽  
Author(s):  
Yixian Zheng ◽  
Zhonghua Liu
Keyword(s):  
Hemogenic Endothelium

3106 – DIRECT INDUCTION OF HEMOGENIC ENDOTHELIUM AND MYELOID CELLS FROM HUMAN IPSCS USING ETV2 MODIFIED RNA

2020 ◽  
Vol 88 ◽  
pp. S71
Author(s):  
Aditi Majumder ◽  
Vera Brok-Volchanskaya ◽  
Igor Slukvin ◽  
David Bennin ◽  
Anna Huttenlocher ◽  
...  
Keyword(s):  
Myeloid Cells ◽  
Hemogenic Endothelium ◽  
Human Ipscs ◽  
Direct Induction

scholarly journals Cell cycle dynamics and complement expression distinguishes mature haematopoietic subsets arising from hemogenic endothelium

Cell Cycle ◽  
2017 ◽  
Vol 16 (19) ◽  
pp. 1835-1847 ◽  
Author(s):  
Joan P. Zape ◽  
Carlos O. Lizama ◽  
Kelly M. Cautivo ◽  
Ann C. Zovein
Keyword(s):  
Cell Cycle ◽  
Hemogenic Endothelium ◽  
Cell Cycle Dynamics

scholarly journals Notch1 acts via Foxc2 to promote definitive hematopoiesis via effects on hemogenic endothelium

Blood ◽  
2015 ◽  
Vol 125 (9) ◽  
pp. 1418-1426 ◽  
Author(s):  
Il Ho Jang ◽  
Yi-Fen Lu ◽  
Long Zhao ◽  
Pamela L. Wenzel ◽  
Tsutomu Kume ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Zebrafish Embryos ◽  
Hemogenic Endothelium ◽  
Key Points ◽  
Definitive Hematopoiesis

Key Points Notch1 induction promotes specification of hemogenic endothelial cells during embryonic stem cell differentiation. Foxc2 functions downstream of Notch in specification of hemogenic endothelium in mouse and zebrafish embryos.

Abstract 356: Scl Represses Cardiogenesis via Distant Enhancers during Hemogenic Endothelium Specification

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Tonis Org ◽  
Dan Duan ◽  
Roberto Ferrari ◽  
Amelie Montel-Hagen ◽  
Ben Van Handel ◽  
...  
Keyword(s):  
Target Genes ◽  
Es Cells ◽  
Bhlh Transcription Factor ◽  
Hemogenic Endothelium ◽  
Master Regulator ◽  
Critical Function ◽  
Hematopoietic Stem ◽  
Mesoderm Development ◽  
Chip Sequencing ◽  
Mouse Es Cells

Understanding the mechanisms directing mesoderm specification holds a great potential to advance the development of cell-based therapies for cardiovascular and blood disorders. The bHLH transcription factor Scl is known as the master regulator of the hematopoietic fate. We recently discovered that, in addition to its critical function in promoting the establishment of hemogenic endothelium during hematopoietic stem/progenitor cell (HS/PC) development, Scl is also required to repress cardiomyogenesis in endothelium in hematopoietic tissues and endocardium in the heart. However, the mechanisms for the cardiac repression have remained unknown. Using ChIP-sequencing and microarray analysis of Flk+ mesoderm differentiated from mouse ES cells, we show that Scl both directly activates a broad gene regulatory network required for hemogenic endothelium and HS/PC development (e.g. Runx1, cMyb, Lyl1, Mef2C, Sox7 etc.), and directly represses transcriptional regulators required for cardiogenesis (e.g. Gata4, Gata6, Myocd, etc.) and mesoderm development (Eomes, Mixl1, Etv2, etc.). Repression of cardiac and mesodermal programs occurs during a short developmental window through Scl binding to distant enhancers, while binding to hematopoietic regulators extends throughout HS/PC and red blood cell development and encompasses both distant and proximal binding sites. We also discovered that, surprisingly, Scl complex partners Gata 1 and 2 are dispensable for hematopoietic vs. cardiac specification and Scl binding to majority of its target genes. Nevertheless, Gata factors co-operate with Scl to activate selected transcription factors that facilitate HS/PC emergence from hemogenic endothelium. These results denote Scl as a true master regulator of hematopoietic vs. cardiac fate choice and suggest a mechanism by which lineage-specific bHLH factors direct the divergence of competing fates.

scholarly journals Cyclic AMP Signaling through Epac Axis Modulates Human Hemogenic Endothelium and Enhances Hematopoietic Cell Generation

2016 ◽  
Vol 6 (5) ◽  
pp. 692-703 ◽  
Author(s):  
Shobhit Saxena ◽  
Roger E. Rönn ◽  
Carolina Guibentif ◽  
Roksana Moraghebi ◽  
Niels-Bjarne Woods
Keyword(s):  
Cyclic Amp ◽  
Hematopoietic Cell ◽  
Cell Generation ◽  
Hemogenic Endothelium ◽  
Cyclic Amp Signaling

scholarly journals Making HSCs in vitro: don’t forget the hemogenic endothelium

Blood ◽  
2018 ◽  
Vol 132 (13) ◽  
pp. 1372-1378 ◽  
Author(s):  
Bradley W. Blaser ◽  
Leonard I. Zon
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Autologous Transplantation ◽  
Cell Types ◽  
Therapeutic Modality ◽  
Hemogenic Endothelium ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Cell Ontogeny

Generating a hematopoietic stem cell (HSC) in vitro from nonhematopoietic tissue has been a goal of experimental hematologists for decades. Until recently, no in vitro–derived cell has closely demonstrated the full lineage potential and self-renewal capacity of a true HSC. Studies revealing stem cell ontogeny from embryonic mesoderm to hemogenic endothelium to HSC provided the key to inducing HSC-like cells in vitro from a variety of cell types. Here we review the path to this discovery and discuss the future of autologous transplantation with in vitro–derived HSCs as a therapeutic modality.

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