Faculty Opinions recommendation of Mitochondrial Stress Restores the Heat Shock Response and Prevents Proteostasis Collapse during Aging.

Author(s):  
Seung-Jae V Lee ◽  
Yoonji Jung
Cell Reports ◽  
2017 ◽  
Vol 21 (6) ◽  
pp. 1481-1494 ◽  
Author(s):  
Johnathan Labbadia ◽  
Renee M. Brielmann ◽  
Mario F. Neto ◽  
Yi-Fan Lin ◽  
Cole M. Haynes ◽  
...  

2018 ◽  
Vol 69 (2) ◽  
pp. 337-340
Author(s):  
Vlad Preluca ◽  
Bogdan Horatiu Serb ◽  
Sanda Marchian ◽  
Diter Atasie ◽  
Mihaela Cernusca Mitariu ◽  
...  

Heat shock inductors have potential as treatment for degenerative and protein misfolding diseases. Dimethyl-sulfoxide is widely used as a solvent in pharmacological screening tests and has been shown to have heat shock induction effects. Transgenic Tg (hsp70l:EGFP-HRAS_G12V)io3(AB) zebrafish larvae were exposed for 24 hours to dimethyl-sulfoxide in concentratios of 0.1-2%, and to moderate heat shock inductors pentoxifylline and tacrolimus. Positive controls were exposed to 35, 38 and 40�C for 20 min, and incubated for 24 h at 28�C. Heat shock response was measured by fluorescence microscopy and signal intensity quantification in FIJI. Dimethyl-sulfoxide caused a dose-dependant increase in fluorescent intensity, but significantly lower compared with exposure to 38 and 40�C. Pentoxifylline and tacrolimus induced a significantly higher increase in fluorescence compared with 0.5% dimethyl-sulfoxide. Thus, although dimethyl-sulfoxide has independent heat shock induction effects, concentrations of up to 0.5% are suitable for heat shock response screening tests.


Sign in / Sign up

Export Citation Format

Share Document