scholarly journals Faculty Opinions recommendation of Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions.

2020 ◽  
Author(s):  
Yousin Suh
Keyword(s):  
Context Specificity ◽  
Base Editing ◽  
Human Apobec3g

scholarly journals Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

BMC Biology ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Zhiquan Liu ◽  
Siyu Chen ◽  
Huanhuan Shan ◽  
Yingqi Jia ◽  
Mao Chen ◽  
...  
Keyword(s):  
Context Specificity ◽  
Base Editing ◽  
Human Apobec3g

scholarly journals Highly precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

2019 ◽  
Author(s):  
Zhiquan Liu ◽  
Siyu Chen ◽  
Huanhuan Shan ◽  
Mao Chen ◽  
Yuning Song ◽  
...  
Keyword(s):  
Cytidine Deaminase ◽  
Context Specificity ◽  
Gene Modification ◽  
Base Editing ◽  
Human Apobec3g ◽  
Activity Window

AbstractCytidine base editors, composed of a cytidine deaminase fused to Cas9 nickase, enable efficient C-to-T conversion in various organisms. However, current base editors can induce unwanted bystander C-to-T conversions when more than one C is present in the activity window of cytidine deaminase, which negatively affects the precision. Here, we develop a new base editor with CC context-specificity using rationally engineered human APOBEC3G, thus significantly reduce unwanted bystander activities. In addition, efficient C-to-T conversion that can further recognize relaxed NG PAMs is achieved by combining an engineered SpCas9-NG variant. These novel base editors with improved precision and targeting scope will expand the toolset for precise gene modification in organisms.

Context specificity of automatic influences of memory.

2018 ◽  
Vol 44 (10) ◽  
pp. 1501-1513 ◽  
Author(s):  
Steven M. Smith ◽  
Justin D. Handy ◽  
Alan Hernandez ◽  
Larry L. Jacoby
Keyword(s):  
Context Specificity

scholarly journals Estimating Copy-Number Proportions: The Comeback of Sanger Sequencing

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 283
Author(s):  
Eyal Seroussi
Keyword(s):  
Copy Number ◽  
Sanger Sequencing ◽  
Cytosine Methylation ◽  
Direct Sequencing ◽  
Information Source ◽  
Gene Copy Number ◽  
Cost Effective ◽  
Gene Copy ◽  
Base Editing ◽  
Recent Developments

Determination of the relative copy numbers of mixed molecular species in nucleic acid samples is often the objective of biological experiments, including Single-Nucleotide Polymorphism (SNP), indel and gene copy-number characterization, and quantification of CRISPR-Cas9 base editing, cytosine methylation, and RNA editing. Standard dye-terminator chromatograms are a widely accessible, cost-effective information source from which copy-number proportions can be inferred. However, the rate of incorporation of dye terminators is dependent on the dye type, the adjacent sequence string, and the secondary structure of the sequenced strand. These variable rates complicate inferences and have driven scientists to resort to complex and costly quantification methods. Because these complex methods introduce their own biases, researchers are rethinking whether rectifying distortions in sequencing trace files and using direct sequencing for quantification will enable comparable accurate assessment. Indeed, recent developments in software tools (e.g., TIDE, ICE, EditR, BEEP and BEAT) indicate that quantification based on direct Sanger sequencing is gaining in scientific acceptance. This commentary reviews the common obstacles in quantification and the latest insights and developments relevant to estimating copy-number proportions based on direct Sanger sequencing, concluding that bidirectional sequencing and sophisticated base calling are the keys to identifying and avoiding sequence distortions.

scholarly journals CRISPR-mediated base editing in mice using cytosine deaminase base editor 4

2021 ◽  
Author(s):  
Salah Adlat ◽  
Farooq Hayel ◽  
Ping Yang ◽  
Yang Chen ◽  
Zin Mar Oo ◽  
...  
Keyword(s):  
Cytosine Deaminase ◽  
Base Editing

scholarly journals Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1288
Author(s):  
Wendy Dong ◽  
Boris Kantor
Keyword(s):  
Large Scale ◽  
Lentiviral Vector ◽  
Clinical Applications ◽  
Scale Production ◽  
Base Editing ◽  
Epigenome Editing ◽  
Vector Systems ◽  
Dividing Cells

CRISPR/Cas technology has revolutionized the fields of the genome- and epigenome-editing by supplying unparalleled control over genomic sequences and expression. Lentiviral vector (LV) systems are one of the main delivery vehicles for the CRISPR/Cas systems due to (i) its ability to carry bulky and complex transgenes and (ii) sustain robust and long-term expression in a broad range of dividing and non-dividing cells in vitro and in vivo. It is thus reasonable that substantial effort has been allocated towards the development of the improved and optimized LV systems for effective and accurate gene-to-cell transfer of CRISPR/Cas tools. The main effort on that end has been put towards the improvement and optimization of the vector’s expression, development of integrase-deficient lentiviral vector (IDLV), aiming to minimize the risk of oncogenicity, toxicity, and pathogenicity, and enhancing manufacturing protocols for clinical applications required large-scale production. In this review, we will devote attention to (i) the basic biology of lentiviruses, and (ii) recent advances in the development of safer and more efficient CRISPR/Cas vector systems towards their use in preclinical and clinical applications. In addition, we will discuss in detail the recent progress in the repurposing of CRISPR/Cas systems related to base-editing and prime-editing applications.

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