COMPLEX ASSOCIATION ANALYSIS OF ANTIOXIDANT GENES POLYMORPHIC DNA-MARKERS WITH AGE IN THE ETHNIC GROUP OF ABKHAZIANS

Author(s):  
В.В. Эрдман ◽  
А.З. Матуа ◽  
Т.Р. Насибуллин ◽  
И.А. Туктарова ◽  
Ф.А. Горухчиева ◽  
...  

Впервые в этнической группе абхазов выполнен анализ ассоциаций полиморфных ДНК-маркеров генов антиоксидантной системы CAT (rs1001179), MSRA (rs10098474), GPX1 (rs1050450), GSR (rs1002149), GSTP1 (rs1695), SOD1 (rs2070424), SOD2 (rs4880), PON1 (rs662), PON2 (rs7493) с возрастом. С использованием ROC-анализа и логистической регрессии установлено, что спектр частот аллелей и генотипов полиморфных маркеров генов PON1 и GSTP1 меняется на протяжении всего исследуемого возрастного периода (21 107 лет); распределение частот аллелей и генотипов по полиморфным маркерам генов CAT и SOD2 изменяется на рубеже 60 лет. Методом Монте-Карло марковскими цепями определены мультилокусные генетические маркеры долголетия. У лиц 60-107 лет статистически значимо повышена частота встречаемости паттернов GSTP 1* G/G + PON 1* G ( OR =6,59, PFDR =0,018) и GSTP 1* G/G + SOD 1 *A ( OR =3,4, PFDR =0,041); аллель GSTP 1* A в разных комбинациях с аллелями PON 1*A, PON2 * C и CAT * C встре чается реже (OR=0,3, PFDR<0,05). For the first time in the ethnic group of Abkhazians, the association analysis of polymorphic DNA-markers of the antioxidant genes CAT ( rs 1001179), MSRA ( rs 10098474), GPX 1 ( rs 1050450), GSR ( rs 1002149), GSTP 1 ( rs 1695), SOD 1 ( rs 2070424), SOD 2 ( rs 4880) , PON 1 ( rs 662), PON 2 ( rs 7493) with age was performed. Using ROC-analysis and logistic regression, it was found that the spectrum of alleles and genotypes frequencies of PON 1 and GSTP 1 genes polymorphic markers change throughout the studied age period (21-107 years old); the distribution of allele and genotype frequencies of CAT and SOD 2 genes polymorphic markers changes within the age of 60 years. Multilocus genetic markers of longevity were determined by the Monte Carlo Markov chain method. Among persons in the age range 60-107 years, the frequency of observation of the patterns GSTP 1* G/G + PON 1* G ( OR =6,59, P FDR=0,018) and GSTP 1* G/G + SOD 1 *A ( OR =3,4, P FDR=0,041) is statistically significantly increased; the GSTP 1* A allele in various combinations with the PON 1* A , PON 2* C and CAT * C alleles are less common ( OR =0,3, P FDR<0,05).

2020 ◽  
Author(s):  
Mingjian Bai ◽  
Shilong Wang ◽  
Ruiqing Ma ◽  
Ying Cai ◽  
Yiyan Lu ◽  
...  

Abstract Background Pseudomyxoma peritonei (PMP) is a rare disease, the prognosis of overall survival (OS) is affected by many factors, present study aim to define independent prediction indicators and establish a nomogram for PMP patients.Methods 119 PMP patients received cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) in our center for the first time were included between 01/06/2013 and 22/11/2019 . The log-rank test was used to compare the OS rate among groups, subsequently, variables with P<0.10 were subjected to multivariate Cox model for defining independent prediction indicators. Finally, the nomogram prediction models will be established and for internal validation.Results Multivariate analysis showed Sex, D-Dimer, CA125, CA19-9, PCI, and degree of radical surgery were independently associated with OS in PMP patients. A nomogram was plotted based on the independent predictive factors and undergone internal validation, ROC analysis was performed to calculate discrimination ability of the nomogram, the C-index was 0.880 (95%CI: 0.806- 0.933) and calibration plots showed good performance. Conclusions Six independent prognostic factor for predicting survival in PMP patients were difined, the nomogram has a good discrimination ability for individual risk predition, more researches are needed to verify and improve the prediction model.


2019 ◽  
Vol 190 ◽  
pp. 5
Author(s):  
Benjamin Musavu Moussavou

A study of Albian bivalves from Madiela Formation in north of Gabonese coastal basin has been carried out from N’Toum quarry geological section. Four genera including six species are identified: Liopistha (Psilomya) sp. 1, ? Liopistha (Psilomya) sp. 2, Megaporomya sp., Neithea (Neithea) dutrugei (Coquand, 1862), Pleuromya sp. 1 and Pleuromya sp. 2. One morphotype is identified as Bivalvia gen. et sp. indet. The genus Megaporomya Ayoub-Hannaa et al., 2013 and all identified species, except Neithea (Neithea) dutrugei (Coquand, 1862), are found for the first time in Gabonese coastal basin. Now, a total of twelve species of bivalves have been reported from the Madiela Formation. In Gabon, the presence for the first time of genera Liopistha and Pleuromya in N’Toum quarry geological section which is assigned to the Albian permits us to expand their geographical distribution until N’Toum region, and their age range to Albian.


2020 ◽  
Vol 2 ◽  
Author(s):  
Michael Romann ◽  
Eva Rüeger ◽  
Mirjam Hintermann ◽  
Raphael Kern ◽  
Oliver Faude

Introduction: Relative age effects (RAEs) refer to the overrepresentation of players born earlier in the selection year compared to late-born players within the same age category. To date, the origins and mechanisms of RAEs are still unclear. To evaluate the development of RAEs in terms of age group and selection level, we analyzed data of all registered child and adolescent football players in Switzerland.Methods: Age category, selection level, and birthdate from all licensed 101,991 Swiss child and youth football players assigned to a specific team [9,149 girls (9.0%) and 92,842 boys (91.0%); age range: 4.6–19.6 years] were analyzed. Additionally, out of 1,128 clubs, 54 clubs provided their documented waiting lists (1,224 players). Birthdate distributions were split by age category, sex, and birth quarter (Q1 = January to March, Q4 = October to December). RAEs were calculated using odds ratios (Q1 vs. Q4) with 95% confidence intervals (95% CI).Results: We found small RAEs among U8 players (OR 1.44 [95% CI 1.31, 1.59]) and U10 (OR 1.24 [95% CI 1.16, 1.32]). The RAE was negligible in all other age categories, independent of gender. In children's football, 5,584 (71.3%) teams performed selections. In teams without selection, there were no obvious RAEs. However, teams with selections for the same age category showed small RAEs with an overrepresentation of Q1 athletes in the first team (OR = 1.29 [95% CI 1.24, 1.35]) and inverse RAEs with an underrepresentation of Q1 athletes in the last team (OR = 0.85 [95% CI 0.82, 0.89]). Only small RAEs were observed on the waiting lists for the U8 (OR = 1.48 [1.13, 1.95]).Discussion and Conclusion: RAEs have a small, but consistent effect on participation in Swiss children's football at the grassroots level. Contrary to expectations, no inverse RAEs were found on the waiting lists. Nonetheless, first time coach selections seem to be the origin of RAEs. To protect young athletes from discrimination, RAE biases should be analyzed and eliminated at all stages of sport participation, selection, and dropout situations. Modifications to the organizational structure of sport and athlete development systems are recommended to prevent RAE-related discrimination in youth sports.


HPB Surgery ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Helge Bruns ◽  
Veronika Kortendieck ◽  
Hans-Rudolf Raab ◽  
Dalibor Antolovic

Background. After pancreaticoduodenectomy (PD), pancreatic fistulas (PF) are a frequent complication. Infusions may compromise anastomotic integrity. This retrospective analysis evaluated associations between intraoperative fluid excess and PF. Methods. Data on perioperative parameters including age, sex, laboratory findings, histology, infusions, surgery time, and occurrence of grade B/C PF was collected from all PD with pancreaticojejunostomy (PJ) performed in our department from 12/2011 till 02/2015. The glomerular filtration rate (GFR), infusion rate, and the ratio of both and its association with PF were calculated. ROC analysis was employed to identify a threshold. Results. Complete datasets were available for 83 of 86 consecutive cases. Median age was 66 years (34–84; 60% male), GFR was 93 mL/min (IQR 78–113), and surgery time was 259 min (IQR 217–307). Intraoperatively, 13.6 mL/min (7–31) was infused. In total, n=18 (21%) PF occurred. When the infusion : GFR ratio exceeded 0.15, PF increased from 11% to 34% (p=0.0157). No significant association was detected for any of the other parameters. Conclusions. This analysis demonstrates for the first time an association between intraoperative fluid excess and PF after PD with PJ even in patients with normal renal function. A carefully patient-adopted fluid management with due regard to renal function may help to prevent postoperative PF.


2006 ◽  
Vol 97 (2) ◽  
pp. 167-211 ◽  
Author(s):  
Charles H. Wellman

ABSTRACTSince the late 1980s an extensive programme of trenching/borehole drilling has been undertaken in order to study the Lower Devonian ‘Lower Old Red Sandstone’ deposits of the Rhynie outlier in the Grampian Highlands of Scotland. The boreholes have provided new information on the stratigraphical succession and geological structure of the Rhynie outlier, both of which were hitherto poorly understood due to the paucity of good surface exposure and the complex geological relationships of the deposits. One hundred and eighteen palynological samples were collected, representing much of the stratigraphical sequence of the inlier, of which 106 were productive. Productive samples yield assemblages of well preserved palynomorphs, dominated by spores and phytodebris, but also containing arthropod cuticle and rare freshwater algal remains. The spore assemblages are systematically described and two new genera and six new species proposed. They are similar throughout the sequence and the spores belong to the polygonalis–emsiensis Spore Assemblage Biozone of Richardson & McGregor (1986) and the PoW Oppel Zone (possibly Su Interval Zone) of Streel et al. (1987), indicating an early (but not earliest) Pragian–?earliest Emsian age range, that may possibly be restricted to latest Pragian–?earliest Emsian. The palynomorph assemblages contain only terrestrial forms, supporting sedimentological interpretation of the deposits as ‘Lower Old Red Sandstone’ fluviatile and lacustrine deposits, with occasional extrusive volcanics and volcaniclastic sediments intercalated. The palynomorphs are of variable thermal maturity (within and between samples), probably reflecting differential heating associated with the complex volcanic/hydrothermal system. The new palynological data provide, for the first time, a reliable biostratigraphical age for the deposits, and suggest that they accumulated relatively rapidly. Spore biostratigraphy and thermal maturity studies facilitate correlation of the tectonically complex deposits, and shed light on other aspects of the geological history of the outlier. The palynomorph assemblages also aid interpretation of the biota of the Rhynie basin, including the exceptionally preserved biotas of the Rhynie and Windyfield cherts.


2021 ◽  
Vol 7 (3) ◽  
pp. 054-057
Author(s):  
Anibor Ese ◽  
Okolugbo Nekwu Emmanuel ◽  
Inikoro Charity ◽  
Odiete Enoh

Objective: The aim of this study is investigating the distribution of earlobe attachment among the Ika ethnic group, in Delta State, Nigeria. Methods: This cross-sectional study was conducted in Ika speaking communities in Delta State, Nigeria. The sample consists of 384 subjects (192 males and 192 females) and the participants were categorized in accordance of sexual category with age ranges from 18 to 60 years. Data was collected by visual observation and the data obtained was analyzed with the aid of Statistical Package for the Social Sciences (SPSS) Version 21.0. A p-value less than 0.05 was considered as statistically significant. Results: The males portrayed the highest frequency distribution of attached earlobe while the females showed more of free earlobe. There is no significant gender difference in the pattern of earlobe attachment (p =.46). Free earlobe manifested among the 39-48years age range with the highest frequency distribution and the least by age interval within 59years and above. The attached earlobe displayed age differences in distribution with the highest frequency from 49-58 age range and the least by age range 59years and above. There is a remarkable age variation in earlobe attachment (p =.001). Conclusion: The attached earlobe is more predominant than the free or unattached earlobe among the Ikas in Delta State, Nigeria. The association between age and pattern of earlobe attachment is significant and there is no significant gender variation in the pattern of earlobe attachment.


Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 503-514 ◽  
Author(s):  
Jalal Gharesouran ◽  
Maryam Rezazadeh ◽  
Mohaddes Mojtaba

Alzheimer's disease is a complex neurodegenerative disorder characterized by memory and cognitive impairment and is the leading cause of dementia in the elderly. The aim of our study was to examine the polymorphic DNA markers CCR2 (+190 G/A), CCR5?32, TNF-? (-308 G/A), TNF-? (-863 C/A) and CALHM1 (+394 C/T) to determine the relationship between these polymorphisms and the risk of late onset Alzheimer's disease in the population of Eastern Azerbaijan of Iran. A total of 160 patient samples and 163 healthy controls were genotyped by PCR-RFLP and the results confirmed using bidirectional sequencing. Statistical analysis of obtained data revealed non-significant difference between frequency of CCR5?32 in case and control groups. The same result was observed for TNF-? (-863 C/A) genotype and allele frequencies. Contrary to above results, significant differences were detected in frequency of TNF-? (-308 G/A) and CCR2-64I genotypes between the cases and healthy controls. A weak significant difference observed between allele and genotype frequencies of rs2986017 in CALHM1 (+394 C/T; P86L) in patient and control samples. It can be concluded that the T allele of P86L variant in CALHM1 & +190 G/A allele of CCR2 have a protective role against abnormal clinical features of Alzheimer's disease.


2021 ◽  
Author(s):  
Yuhe Wang ◽  
Zhongtao Wang ◽  
Xue He ◽  
Li Wang ◽  
Mei Bai ◽  
...  

Abstract Background: Tuberculosis (TB) is an important health issue in our world. It is reported that various factors may effect on its pathogenesis. In this current study, we aimed to investigate the association between SLC11A1 polymorphism and the risk of TB among 510 TB patients and 508 healthy controls.Methods: Agena MassARRAY platform was conducted for genotyping. Odds ratios (ORs) and 95% confidence intervals (CIs) were analyzed through unconditional logistic regression adjustment confound factors, such as age and gender.Results: The results suggested that the allele and genotype frequencies of polymorphisms in SLC11A1 were not observed associated with TB risk. Subsequently, stratified analysis by age and gender confirmed that rs7608307 “A/A” and “C/T-T/T” genotypes were related with increased TB risk in age ≤ 41 group (p = 0.021) and males (p = 0.013), respectively. Besides, rs13062 “A/A” genotype was reduced TB risk in age > 41 group (p = 0.043). In addition, we observed that the “C/C” genotype of rs4674301 was noteworthy correlated with increased TB risk in females (p = 0.043). Conclusion: Our results demonstrated the relationship between SLC11A1 polymorphism and TB risk and confirmed for the first time that the correlation was restricted to age and gender in northwest Chinese population.


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