CHANGES IN THE NUMBER OF p23-POSITIVE FIBROBLASTS IN HUMAN DERMIS WITH AGING

2021 ◽  
pp. 694-700
Author(s):  
А.Г. Гунин ◽  
Н.Н. Голубцова
Keyword(s):  
Old Age ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Immunohistochemical Technique ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis ◽  
Related Increase

Целью работы стало исследование содержания белка p23 в фибробластах дермы человека от 20 нед беременности до 85 лет, а также определение участия p23 для возрастных изменений численности и пролиферации фибробластов в дерме человека. p23, ядерный антиген пролиферирующих клеток (PCNA), маркер фибробластов виментин выявляли в срезах кожи непрямым иммуногистохимическим методом. В дерме человека от 20 нед беременности до 85 лет наблюдали планомерное увеличение доли фибробластов, имеющих положительную окраску на p23. Возрастное изменение численности p23позитивных фибробластов в дерме достоверно коррелирует с возрастным уменьшением общей численности и доли PCNA-позитивных фибробластов в дерме человека, что свидетельствует об участии p23 в возрастных изменениях общей численности и доли пролиферирующих фибробластов в дерме человека. The aim of this work was to examine the content of p23 in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of p23 in age-dependent changes in the number and proliferation of fibroblasts in the dermis. p23, proliferating cells nuclear antigen (PCNA), fibroblasts marker vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for p23 in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old. Age-related increase in a portion of fibroblasts with positive staining for p23 is significantly correlated with an age-related decrease in total number and percent of PCNA positive fibroblasts in human dermis. Age-related increase in the content of p23 in fibroblasts is involved in an age-dependent decrease in their total number and proliferation in the dermis.

ARYLHYDROCARBON RECEPTOR NUCLEAR TRANSLOCATOR () IN HUMAN SKIN DURING AGING

2020 ◽  
pp. 313-318
Author(s):  
А. Г. Гунин ◽  
Н. Н. Голубцова ◽  
Н. К. Корнилова
Keyword(s):  
Dermal Fibroblast ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis ◽  
Group A ◽  
Arylhydrocarbon Receptor

Целью настоящей работы стало исследование содержания ядерного транслокатора арил-гидрокарбонового рецептора ( ARNT ) в фибробластах дермы человека от 20 нед беременности до 85 лет, а также определение значения ARNT для возрастных изменений численности фибробластов в дерме человека. ARNT , ядерный антиген пролиферирующих клеток ( PCNA ) выявляли в срезах кожи непрямым иммуногистохимическим методом. Результаты показали, что доля фибробластов дермы с положительной окраской на ARNT постепенно снижается от 20 нед беременности до 40 лет. С 41 года происходит резкое увеличение доли фибробластов дермы с положительной окраской на ARNT, достигая максимума в возрастной группе 61-85 лет. Общее количество и доля PCNA -положительных фибробластов в дерме уменьшается с возрастом, наиболее значимо - с антенатального периода до 40 лет. Возрастные изменения содержания ARNT в фибробластах дермы статистически не связаны с возрастным уменьшением численности общего количества и процента PCNA -положительных фибробластов в дерме. The aim of this work was to examine the content of arylhydrocarbon receptor nuclear translocator ( ARNT ) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of ARNT in age-dependent changes in the number of fibroblasts in the dermis. ARNT , proliferating cells nuclear antigen ( PCNA ) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for ARNT in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of ARNT positive fibroblasts in dermis is increased sufficiently since 41 year old until 60-85 years old group. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Age-related changes in the content of ARNT in fibroblasts is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts the dermis.

HEAT SHOCK PROTEIN 90 (90) IN AGE-DEPENDENT CHANGES IN THE NUMBER OF FIBROBLASTS IN HUMAN SKIN

2020 ◽  
pp. 40-45
Author(s):  
А. Г. Гунин ◽  
Н. Н. Голубцова ◽  
Н. К. Корнилова
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis ◽  
Hsp 90

Целью работы стало исследование содержания белка теплового шока 90 ( HSP 90) в фибробластах дермы человека от эмбрионального развития и до глубокой старости (от 20 нед беременности до 85 лет), а также определение значения HSP 90 для возрастных изменений численности фибробластов в дерме человека. HSP 90, ядерный антиген пролиферирующих клеток ( PCNA ) выявляли в срезах кожи непрямым иммуногистохимическим методом. Результаты показали, что в коже человека от 20 нед беременности до 20 лет доля фибробластов дермы с положительной окраской на HSP 90 остается постоянной. С 21 года до 60 лет наблюдают планомерное уменьшение доли фибробластов дермы, имеющих положительную окраску на HSP 90. У людей 61-85 лет происходит резкое увеличение доли фибробластов дермы с положительной окраской на HSP 90. Возрастные изменения содержания HSP 90 положительных фибробластов в дерме статистически не связаны с возрастным уменьшением общего количества и доли PCNA -положительных фибробластов в дерме. The aim of this work was to examine the content of heat shock protein 90 ( HSP 90) in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of HSP 90 in age-dependent changes in the number of fibroblasts in the dermis. HSP 90, proliferating cells nuclear antigen ( PCNA ) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for HSP 90 in the dermis is not changed from 20 weeks of development to 20 years old. Percent of HSP 90 positive fibroblasts in dermis is decreased from 21 to 60 years old. From 61 year, the number of HSP 90 positive fibroblasts in dermis is increased. Age-related changes in the number of HSP 90 positive fibroblasts is not statistically associated with an age-related decrease in a total number and percent of PCNA positive fibroblasts the dermis.

ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN () IN HUMAN DERMIS DURING AGING

2020 ◽  
pp. 444-449
Author(s):  
А. Г. Гунин ◽  
Н. Н. Голубцова
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Dermal Fibroblast ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Interacting Protein ◽  
Aryl Hydrocarbon ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis

Целью настоящей работы стало исследование содержания белка, ассоциированного с арилгидрокарбоновым рецептором, - aryl hydrocarbon receptor interacting protein ( AIP ), в фибробластах дермы человека от 20 нед беременности до 85 лет, а также определение значения AIP для возрастных изменений численности фибробластов в дерме человека. AIP и ядерный антиген пролиферирующих клеток ( PCNA ) выявляли в срезах кожи непрямым иммуногистохимическим методом. Результаты показали, что в коже человека от 20 нед беременности до 85 лет доля фибробластов дермы с положительной окраской на AIP планомерно возрастает. Общее количество и доля PCNA -положительных фибробластов в дерме уменьшались с возрастом. Наиболее значительное возрастное уменьшение общего числа и PCNA -положительных фибробластов в дерме наблюдали с антенатального периода до 40 лет. Корреляционный анализ показал, что возрастное уменьшение общего числа фибробластов и уменьшение их пролиферативной активности достоверно связаны с увеличением процента фибробластов с положительной окраской на AIP . Результаты позволяют предположить, что AIP участвует в возрастном уменьшении численности и пролиферации фибробластов в дерме человека. The aim of this work was to examine the content of aryl hydrocarbon receptor interacting protein ( AIP ) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of AIP in age-dependent changes in the number of fibroblasts in the dermis. AIP , proliferating cells nuclear antigen ( PCNA ) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for AIP in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Correlation analysis showed that both age-dependent decrease in the number of fibroblasts and retardation of their proliferation are significantly associated with age-related increase in the number of AIP positive fibroblasts in dermis. Results allow to suggest that AIP is involved in age-dependent decrease in the number and proliferation of fibroblasts in human dermis.

Concrete and Formal Operational thought Processes in Young Adulthood and Old Age

1976 ◽  
Vol 7 (3) ◽  
pp. 237-245 ◽  
Author(s):  
Vivian Clayton ◽  
Willis F. Overton
Keyword(s):  
Old Age ◽  
Living Arrangements ◽  
Fluid Intelligence ◽  
Age Groups ◽  
Thought Processes ◽  
Crystallized Intelligence ◽  
Age Related ◽  
Concrete Operational ◽  
Fluid And Crystallized Intelligence

A study was conducted to examine the role of concrete and formal operations in a young and old population. In addition, the present study explored the relation between operational thought and Cattell's concept of fluid and crystallized intelligence, as well as the role of differential living arrangements in maintaining operational thought. Eighty females from three age groups (18–20 years, 60–70 years and 70–80 years of age) were tested on a series of Piagetian tasks and indices of fluid and crystallized intelligence. The findings supported the notion that age-related performance differences occur in the area of formal operational thought prior to the time they occur in concrete operational thought. Except for the young sample, the operational tasks were found to be unrelated to fluid intelligence at the age levels represented in this study. Living independently as opposed to living in an old age home did not appear to be a significant factor in maintaining operational thought. Discussion focused on the necessity of identifying those factors which influence the developmental course of formal operational thought across the life span.

Effect of age and gender on reference intervals of red blood cell distribution width (RDW) and mean red cell volume (MCV)

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
Johannes J.M.L. Hoffmann ◽  
Karin C.A.M. Nabbe ◽  
Nicole M.A. van den Broek
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Reference Intervals ◽  
Cell Distribution ◽  
Age Dependency ◽  
Distribution Width ◽  
Age Related ◽  
Age Dependent ◽  
Red Blood Cell Distribution ◽  
Related Increase

AbstractRed blood cell distribution width (RDW) was recently shown to be age-dependent when using Sysmex XE-2100 hematology analyzers. As measuring RDW is subject to technology, we have investigated if this relation also exists when using a different hematology analyzer, Abbott CELL-DYN Sapphire. In addition, as RDW is generally expressed relative to mean red blood cell volume (MCV), we have explored how MCV influences the age-dependency of RDW.We measured RDW and MCV in a large cohort and calculated RDW-SD (the “absolute” RDW), which does not contain MCV. For establishing reference intervals we used Bhattacharya statistics.In our study cohort of 8089 individuals we found a strong association between RDW and age, but not with gender. Also MCV showed an age-related increase over the entire age range. The conventional RDW increased by 6% from the youngest to oldest age class, whereas RDW-SD increased by nearly 15%. This difference was caused by a mean age-related increase in MCV of 6.6%. Age-dependent reference intervals were established for RDW, RDW-SD and MCV.The age-dependency of RDW seems to be a universal biological feature rather than related with a single type of hematology analyzer. As not only RDW, but also MCV increases with age, we propose that future studies on the prognostic significance of RDW take its age-dependency into account and focus on RDW-SD as a potential marker of adverse events in many clinical conditions.

Effects of elevated fetal cortisol concentrations on the volume, secretion, and reabsorption of lung liquid

1995 ◽  
Vol 269 (4) ◽  
pp. R881-R887 ◽  
Author(s):  
M. J. Wallace ◽  
S. B. Hooper ◽  
R. Harding
Keyword(s):  
Plasma Cortisol ◽  
Fetal Lung ◽  
Liquid Volume ◽  
Fetal Sheep ◽  
Fetal Plasma ◽  
Age Related ◽  
Lung Liquid ◽  
Secretion Rates ◽  
Related Increase

We have examined the role of cortisol in the gestational age-related increase in the ability of epinephrine to inhibit the secretion and induce the reabsorption of fetal lung liquid. Chronically catheterized fetal sheep were infused with either saline (n = 6) or increasing doses of cortisol (1.5-3.5 mg/day; n = 6) between 120 and 130 days of gestation (term approximately 145 days). Lung liquid volumes and secretion rates were measured at 120 days (before infusion) and at 125 days, and then at 130 days we tested the ability of epinephrine to inhibit lung liquid secretion and induce liquid reabsorption. Cortisol infusions increased fetal plasma cortisol and 3,5,3'-triiodothyronine (T3) concentrations to levels observed just before labor and significantly increased the age-related increase in fetal lung liquid volume and secretion rate. At 130 days, epinephrine caused a significantly greater rate of lung liquid reabsorption in cortisol-infused fetuses (10.3 +/- 2.3 ml/h) than in saline-infused fetuses (1.5 +/- 1.6 ml/h). We conclude that a premature elevation in circulating fetal cortisol concentrations, probably in conjunction with elevated T3 concentrations, prematurely increases the epinephrine-induced reabsorption of fetal lung liquid. It is likely, therefore, that the preparturient increase of fetal cortisol concentrations plays an important role in the clearance of lung liquid at birth.

Age-dependent alterations in Ca2+ homeostasis: role of TRPV5 and TRPV6

2006 ◽  
Vol 291 (6) ◽  
pp. F1177-F1183 ◽  
Author(s):  
Monique van Abel ◽  
Sylvie Huybers ◽  
Joost G. J. Hoenderop ◽  
Annemiete W. C. M. van der Kemp ◽  
Johannes P. T. M. van Leeuwen ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mrna Levels ◽  
Serum Osteocalcin ◽  
Intestinal Epithelial ◽  
Receptor Mrna ◽  
Age Related ◽  
Age Dependent ◽  
Elder People ◽  
Age Related Changes

Aging is associated with alterations in Ca2+ homeostasis, which predisposes elder people to hyperparathyroidism and osteoporosis. Intestinal Ca2+ absorption decreases with aging and, in particular, active transport of Ca2+ by the duodenum. In addition, there are age-related changes in renal Ca2+ handling. To examine age-related changes in expression of the renal and intestinal epithelial Ca2+ channels, control (TRPV5+/+) and TRPV5 knockout (TRPV5−/−) mice aged 10, 30, and 52 wk were studied. Aging of TRPV5+/+ mice resulted in a tendency toward increased renal Ca2+ excretion and significantly decreased intestinal Ca2+ absorption, which was accompanied by reduced expression of TRPV5 and TRPV6, respectively, despite increased serum 1,25(OH)2D3 levels. Similarly, in TRPV5−/− mice the existing renal Ca2+ loss was more pronounced in elder animals, whereas the compensatory intestinal Ca2+ absorption and TRPV6 expression declined with aging. In both mice strains, aging resulted in a resistance to 1,25(OH)2D3 and diminished renal vitamin D receptor mRNA levels, whereas serum Ca2+ levels remained constant. Furthermore, 52-wk-old TRPV5−/− mice showed severe hyperparathyroidism, whereas PTH levels in elder TRPV5+/+ mice remained normal. In 52-wk-old TRPV5−/− mice, serum osteocalcin levels were increased in accordance with the elevated PTH levels, suggesting an increased bone turnover in these mice. In conclusion, downregulation of TRPV5 and TRPV6 is likely involved in the impaired Ca2+ (re)absorption during aging. Moreover, TRPV5−/− mice likely develop age-related hyperparathyroidism and osteoporotic characteristics before TRPV5+/+ mice, demonstrating the importance of the epithelial Ca2+ channels in Ca2+ homeostasis.

scholarly journals Impaired peroxisomal import in Drosophila hepatocyte-like cells induces cardiac dysfunction through the pro-inflammatory cytokine Upd3

2019 ◽  
Author(s):  
Kerui Huang ◽  
Ting Miao ◽  
Kai Chang ◽  
Ping Kang ◽  
Qiuhan Jiang ◽  
...  
Keyword(s):  
Cardiac Dysfunction ◽  
Inflammatory Cytokine ◽  
Cytokine Signaling ◽  
Over Expression ◽  
Autonomous Regulation ◽  
Age Related ◽  
Evolutionarily Conserved ◽  
Age Dependent ◽  
Import Receptor

AbstractAge is a major risk factor for cardiovascular diseases. Currently, the non-autonomous regulation of age-related cardiac dysfunction is poorly understood. In the present study, we discover that age-dependent induction of cytokine unpaired 3 (Upd3) in Drosophila oenocytes (hepatocyte-like cells), due to a dampened peroxisomal import function, is the primary non-autonomous mechanism for elevated arrhythmicity in old hearts. We show that Upd3 is significantly up-regulated (52-fold) in aged oenocytes. Oenocyte-specific knockdown of Upd3 is sufficient to block aging-induced cardiac arrhythmia. We further show that the age-dependent induction of Upd3 is triggered by impaired peroxisomal import and elevated JNK signaling in aged oenocytes. Intriguingly, oenocyte-specific over-expression of Pex5, the key peroxisomal import receptor, restores peroxisomal import, blocks age-related Upd3 induction, and alleviates aging- and paraquat-induced cardiac arrhythmicity. Thus, our studies identify an important role of the evolutionarily conserved pro-inflammatory cytokine signaling and hepatocyte-specific peroxisomal import in mediating non-autonomous regulation of cardiac aging.

scholarly journals Immunosenescence in Choroidal Neovascularization (CNV)—Transcriptional Profiling of Naïve and CNV-Associated Retinal Myeloid Cells during Aging

2021 ◽  
Vol 22 (24) ◽  
pp. 13318
Author(s):  
Anja Schlecht ◽  
Adrian Thien ◽  
Julian Wolf ◽  
Gabriele Prinz ◽  
Hansjürgen Agostini ◽  
...  
Keyword(s):  
Choroidal Neovascularization ◽  
Transcriptional Profiling ◽  
Myeloid Cells ◽  
Age Related Macular Degeneration ◽  
Physiological Aging ◽  
Future Studies ◽  
Signature Genes ◽  
Age Related ◽  
Age Dependent

Immunosenescence is considered a possible factor in the development of age-related macular degeneration and choroidal neovascularization (CNV). However, age-related changes of myeloid cells (MCs), such as microglia and macrophages, in the healthy retina or during CNV formation are ill-defined. In this study, Cx3cr1-positive MCs were isolated by fluorescence-activated cell sorting from six-week (young) and two-year-old (old) Cx3cr1GFP/+ mice, both during physiological aging and laser-induced CNV development. High-throughput RNA-sequencing was performed to define the age-dependent transcriptional differences in MCs during physiological aging and CNV development, complemented by immunohistochemical characterization and the quantification of MCs, as well as CNV size measurements. These analyses revealed that myeloid cells change their transcriptional profile during both aging and CNV development. In the steady state, senescent MCs demonstrated an upregulation of factors contributing to cell proliferation and chemotaxis, such as Cxcl13 and Cxcl14, as well as the downregulation of microglial signature genes. During CNV formation, aged myeloid cells revealed a significant upregulation of angiogenic factors such as Arg1 and Lrg1 concomitant with significantly enlarged CNV and an increased accumulation of MCs in aged mice in comparison to young mice. Future studies need to clarify whether this observation is an epiphenomenon or a causal relationship to determine the role of immunosenescence in CNV formation.

scholarly journals The breadth of attention in old age

2012 ◽  
Vol 3 (1) ◽  
pp. 10 ◽  
Author(s):  
Stefanie Hüttermann ◽  
Otmar Bock ◽  
Daniel Memmert
Keyword(s):  
Older Adults ◽  
Visual Field ◽  
Old Age ◽  
Attention Deficits ◽  
Eye Position ◽  
Visual Periphery ◽  
Age Related ◽  
Breadth Of Attention

Older adults typically have more difficulties than younger ones in situations that require attention in the visual periphery, such as driving a car or riding a bicycle. Previous studies accordingly found that the breadth of attention decreases in old age when one attention-demanding task is presented at fixation and simultaneously another one in the visual periphery. The present work evaluates the role of eye position for the observed deficit by presenting both tasks in the visual periphery (condition peripheral-peripheral) or by leaving it up to the subjects where in the visual field the tasks appear (condition free-gaze). Our data indicate that attention breadth decreases by 27% from the age of early 20 to the age of late 60 in both conditions. This outcome generalizes previous findings about age-related attention deficits to scenarios that were not explored in previous studies, yet are relevant for everyday behavior.

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