Effect of all-trans retinoic acid on the expression of epithelial-mesenchymal transition-related molecules in human malignant melanoma A375 cells

2020 ◽  
Keyword(s):  
Retinoic Acid ◽  
Malignant Melanoma ◽  
Epithelial Mesenchymal Transition ◽  
Human Malignant Melanoma ◽  
Mesenchymal Transition ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
A375 Cells

scholarly journals In Vitro Anticancer Effects of All-trans Retinoic Acid in Combination with Dacarbazine against CD117+ Melanoma Cells

Drug Research ◽  
2020 ◽  
Vol 70 (12) ◽  
pp. 563-569
Author(s):  
Bahareh Mohammadi Jobani ◽  
Elham Mohebi ◽  
Nowruz Najafzadeh
Keyword(s):  
Cell Cycle ◽  
Retinoic Acid ◽  
Malignant Melanoma ◽  
Cell Cycle Arrest ◽  
Combined Treatment ◽  
Melanoma Cells ◽  
Cycle Arrest ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid

Abstract Background Malignant melanoma is a common form of skin cancer that contains different cell types recognized by various cell surface markers. Dacarbazine-based combination chemotherapy is frequently used for the treatment of melanoma. Despite its potent anticancer properties, resistance to dacarbazine develops in malignant melanoma. Here, we aim to improve response to dacarbazine therapy by pretreatment with all-trans retinoic acid (ATRA) in CD117+ melanoma cells. Methods The CD117+ melanoma cells were sorted from A375 malignant melanoma cell line using magnetic-activated cell sorting (MACS). The cell viability was examined by cell proliferation assay (MTT). Apoptosis was determined by acridine orange/ ethidium bromide staining. Indeed, we performed flow cytometry to evaluate the cell cycle arrest. Results Here, the CD117+ melanoma cells were incubated with various concentrations of ATRA, dacarbazine, and their combination to determine IC50 values. We found that 20 µM ATRA treatment followed by dacarbazine was found to be more effective than dacarbazine alone. There was an indication that the combination of ATRA with dacarbazine (ATRA/dacarbazine) caused more apoptosis and necrosis in the melanoma cells (P<0.05). Furthermore, ATRA/dacarbazine treatment inhibited the cell at the G0/G1 phase, while dacarbazine alone inhibited the cells at S phase. Conclusion Collectively, combined treatment with ATRA and dacarbazine induced more apoptosis and enhanced the cell cycle arrest of CD117+ melanoma cells. These results suggested that ATRA increased the sensitivity of melanoma cells to the effect of dacarbazine.

scholarly journals The Role of ATRA, Natural Ligand of Retinoic Acid Receptors, on EMT-Related Proteins in Breast Cancer: Minireview

2021 ◽  
Vol 22 (24) ◽  
pp. 13345
Author(s):  
Pavel Bobal ◽  
Marketa Lastovickova ◽  
Janette Bobalova
Keyword(s):  
Breast Cancer ◽  
Retinoic Acid ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Natural Ligand ◽  
All Trans Retinoic Acid ◽  
Specific Proteins ◽  
Diagnostic Approaches ◽  
Related Proteins ◽  
The Impact

The knowledge of the structure, function, and abundance of specific proteins related to the EMT process is essential for developing effective diagnostic approaches to cancer with the perspective of diagnosis and therapy of malignancies. The success of all-trans retinoic acid (ATRA) differentiation therapy in acute promyelocytic leukemia has stimulated studies in the treatment of other tumors with ATRA. This review will discuss the impact of ATRA use, emphasizing epithelial-mesenchymal transition (EMT) proteins in breast cancer, of which metastasis and recurrence are major causes of death.

Antiproliferative effect of all-trans-retinoic acid in cholangiocarcinoma

2017 ◽  
Author(s):  
A Prawan ◽  
S Butsri ◽  
V Kukongviriyapan ◽  
L Senggunprai ◽  
S Kongpetch
Keyword(s):  
Retinoic Acid ◽  
Antiproliferative Effect ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid
Sign in / Sign up

Export Citation Format

Share Document