STUDY OF GENETIC DIVERSITY USING MICROSATELLITE MARKERS IN IRAQI SHEEP BREEDS

Genotypes and allele frequency values were studied to determine the genetic diversity of three microsatellite markers (BM1329, BM134 and CSSM66) in two local Iraqi sheep breeds (50 Awasi ewes (AW) and 50 Naimi ewes (NA)) and breed in one Agricultural Stations in middle of Al-Furut Iraq (Karbala Governorate). The Genetic analyses for the study were carried out at the Biotechnology Research Center / Al-Nahrain University using PCR technology to obtain alleles for the studied microsatellites. The results of this study indicate that there is a multiplicity of genotypes and alleles resulting from these markers among the members of the single breed and between the studied breeds. It was revealed that the allele frequency values showed the presence of alleles dominant on the rest of alleles produce in this sample studied, the BM1329 microsatellite of the Awassi breed produced the dominant allele frequency in 195(0.25) and 320 (0.25), While in the Naimi breed were the values of the dominant allele frequency of the195(0.22), 220(0.21) and 230(0.24), and the dominant allelic frequency of (BM134) marker in the Awassi breed was 118 and 135 and repeated (0.21 and 0.26, respectively). For CSSM66 microsatellite, the dominant allelic results and their allelic frequency values were (195 (0.20), 205 (0.23) and 220 (0.25)) for Awasi breed, whereas it was 180 (0.24), 195 (0.28) and 220 (0.22) for Nuaimi breed. These results enable us to use these markers as a means of determining the diversity and genetic relationships between individuals of a single breed as well as between breeds.

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Genetic diversity in table grapes based on RAPD and microsatellite markers

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2011000900010 ◽

2011 ◽

Vol 46 (9) ◽

pp. 1035-1044 ◽

Cited By ~ 9

Author(s):

Patrícia Coelho de Souza Leão ◽

Sérgio Yoshimitsu Motoike

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Relationships ◽

Similarity Index ◽

Genetic Distances ◽

Table Grape ◽

Group Method ◽

Molecular Characteristics ◽

Table Grapes ◽

Pair Group

The objective of this work was to analyze the genetic diversity of 47 table grape accessions, from the grapevine germplasm bank of Embrapa Semiárido, using 20 RAPD and seven microsatellite markers. Genetic distances between pairs of accessions were obtained based on Jaccard's similarity index for RAPD data and on the arithmetic complement of the weighted index for microsatellite data. The groups were formed according to the Tocher's cluster analysis and to the unweighted pair‑group method with arithmetic mean (UPGMA). The microsatellite markers were more efficient than the RAPD ones in the identification of genetic relationships. Information on the genetic distance, based on molecular characteristics and coupled with the cultivar agronomic performance, allowed for the recommendation of parents for crossings, in order to obtain superior hybrids in segregating populations for the table grape breeding program of Embrapa Semiárido.

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Assessing genetic diversity and differentiation in Portuguese coarse-wool sheep breeds with microsatellite markers

Small Ruminant Research ◽

10.1016/j.smallrumres.2008.04.006 ◽

2008 ◽

Vol 78 (1-3) ◽

pp. 32-40 ◽

Cited By ~ 27

Author(s):

F. Santos-Silva ◽

R.S. Ivo ◽

M.C.O. Sousa ◽

M.I. Carolino ◽

C. Ginja ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Sheep Breeds

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Genetic diversity of Czech apple cultivars inferred from microsatellite markers analysis

Horticultural Science ◽

10.17221/218/2011-hortsci ◽

2012 ◽

Vol 39 (No. 4) ◽

pp. 149-157 ◽

Cited By ~ 10

Author(s):

J. Patzak ◽

F. Paprštein ◽

A. Henychová ◽

J. Sedlák

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Simple Sequence Repeat ◽

Cultivar Identification ◽

Genetic Relationships ◽

Similarity Coefficient ◽

Collection Management ◽

Apple Cultivars ◽

Repeat Primer ◽

Simple Sequence

Genetic diversity and genetic relationships of Czech apple cultivars were evaluated. Trees of 33 Czech apple cultivars and 97 reference foreign cultivars were analysed using the set of 10 SSR (simple sequence repeat) primer pairs. The total of 89 polymorphic alleles were amplified, while the number of alleles per locus ranged from 4 to 14. The SSR dendrogram, based on the Jaccard’s similarity coefficient, divided apple cultivars into three major groups: Cox’s Orange Pippin, McIntosh and Golden Delicious ancestries. The clustering highly depended on pedigree and origin of apple cultivars. Spontaneous mutated cultivars were identical with their progenitors. We proved that microsatellite markers were useful for evaluation of genetic resources, collection management and cultivar identification.  

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Study of genetic differences among Slovak Tsigai populations using microsatellite markers

Czech Journal of Animal Science ◽

10.17221/1670-cjas ◽

2009 ◽

Vol 54 (No. 10) ◽

pp. 468-474 ◽

Cited By ~ 3

Author(s):

S. Kusza ◽

E. Gyarmathy ◽

J. Dubravska ◽

I. Nagy ◽

A. Jávor ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Genetic Distance ◽

Microsatellite Markers ◽

Mating System ◽

Population Differentiation ◽

Genetic Relationships ◽

The Other ◽

Potential Risks ◽

Substantial Degree

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of <I>F</I><sub>ST</sub> (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.

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Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers

Canadian Journal of Animal Science ◽

10.4141/cjas2011-125 ◽

2012 ◽

Vol 92 (4) ◽

pp. 417-423 ◽

Cited By ~ 2

Author(s):

Jinjun Li ◽

Qingyuan Yuan ◽

Junda Shen ◽

Zhengrong Tao ◽

Guoqing Li ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Genetic Differentiation ◽

Genetic Distance ◽

Microsatellite Markers ◽

Genetic Relationships ◽

Evolutionary Relationships ◽

Distance Estimate ◽

Cluster Analyses ◽

Nei's Genetic Distance

Li, J., Yuan, Q., Shen, J., Tao, Z., Li, G., Tian, Y., Wang, D., Chen, L. and Lu, L. 2012. Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers. Can. J. Anim. Sci. 92: 417–423. The aim of this study was to determine the genetic diversity and evolutionary relationships among five indigenous Chinese goose breeds and one introduced goose breed using 29 microsatellite markers. A total of 334 distinct alleles were observed across the six breeds, and 45 of the 334 alleles (13.5%) were unique to only one breed. The indigenous geese showed higher diversity in terms of the observed number of alleles per locus (4.48–5.90) and observed heterozygosity (0.46–0.53) compared with the introduced breed (3.97 and 0.29, respectively). The pairwise genetic differentiation (FST) between the six goose breeds ranged from 0.04 between Panshi Grey goose (PS) and Yongkang Grey goose to 0.47 between PS and Landes goose; similarly, Nei's genetic distance varied between 0.25 and 0.75. However, the FST between the indigenous Chinese goose breeds was very small. In addition, genetic distance estimate, phylogenic, and cluster analyses of the genetic relationships and population structure revealed that some indigenous goose breeds had hybridized more frequently, resulting in a loss of genetic distinctiveness.

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Analysis of the genetic diversity between Gentile di Puglia, Sopravissana and Sarda sheep breeds using microsatellite markers

Italian Journal of Animal Science ◽

10.4081/ijas.2006.73 ◽

2006 ◽

Vol 5 (1) ◽

pp. 73-78 ◽

Cited By ~ 8

Author(s):

Bianca Moioli ◽

Francesco Napolitano ◽

Luigi Orrù ◽

Gennaro Catillo

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Sheep Breeds

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Transferability and Characterization of Microsatellite Markers from Byrsonima Cydoniifolia A. Juss. (MALPIGHIACEAE) in Seven Related Taxa from Cerrado Biome Reveal Genetic Relationships

10.21203/rs.3.rs-238340/v1 ◽

2021 ◽

Author(s):

Vanessa Bernardes ◽

Devanir M. Murakami ◽

Nair Bizão ◽

Tamara N. Souza ◽

Marcos J. da Silva ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Divergence ◽

High Efficiency ◽

Population Studies ◽

Genetic Relationships ◽

Species Occurrence ◽

Genetic Population ◽

Expected Heterozygosity

Abstract Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-amplification as successfully optimized with high efficiency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10− 6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

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Microsatellite Markers Developed from `Bluecrop' Reveal Polymorphisms in the Genus Vaccinium and Are Suitable for Cultivar Fingerprinting

HortScience ◽

10.21273/hortsci.40.4.1122b ◽

2005 ◽

Vol 40 (4) ◽

pp. 1122B-1122 ◽

Cited By ~ 1

Author(s):

Peter Boches ◽

Lisa J. Rowland ◽

Kim Hummer ◽

Nahla V. Bassil

Keyword(s):

Genetic Diversity ◽

Cluster Analysis ◽

Genetic Distance ◽

Microsatellite Markers ◽

Genetic Relationships ◽

Distance Measures ◽

Hybrid Origin ◽

Pedigree Information ◽

Species Relationships ◽

Ssr Loci

Microsatellite markers for blueberry (Vaccinium L.) were created from a preexisting blueberry expressed sequence tag (EST) library of 1305 sequences and a microsatellite-enriched genomic library of 136 clones. Microsatellite primers for 65 EST-containing simple sequence repeats (SSRs) and 29 genomic SSR were initially tested for amplification and polymorphism on agarose gels. Potential usefulness of these SSRs for estimating species relationships in the genus was assessed through cross-species transference of 45 SSR loci and cluster analysis using genetic distance values from five highly polymorphic EST-SSR loci. Cross-species amplification for 45 SSR loci ranged from 17% to 100%, and was 83% on average in nine sections. Cluster analysis of 59 Vaccinium species based on genetic distance measures obtained from 5 EST-SSR loci supported the concept of V. elliotii Chapm. as a genetically distinct diploid highbush species and indicated that V. ashei Reade is of hybrid origin. Twenty EST-SSR and 10 genomic microsatellite loci were used to determine genetic diversity in 72 tetraploid V. corymbosum L. accessions consisting mostly of common cultivars. Unique fingerprints were obtained for all accessions analyzed. Genetic relationships, based on microsatellites, corresponded well with known pedigree information. Most modern cultivars clustered closely together, but southern highbush and northern highbush cultivars were sufficiently differentiated to form distinct clusters. Future use of microsatellites in Vaccinium will help resolve species relationships in the genus, estimate genetic diversity in the National Clonal Germplasm Repository (NCGR) collection, and confirm the identity of clonal germplasm accessions.

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Assessment of genetic diversity in main local sheep breeds from Romania using microsatellite markers

Archives Animal Breeding ◽

10.5194/aab-63-53-2020 ◽

2020 ◽

Vol 63 (1) ◽

pp. 53-59 ◽

Cited By ~ 2

Author(s):

Andreea Dudu ◽

Gina-Oana Popa ◽

Elena Ghiță ◽

Rodica Pelmuș ◽

Cristina Lazăr ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Animal Husbandry ◽

High Rate ◽

Farm Animals ◽

Mountain Areas ◽

Breeding Programs ◽

Sheep Breeds ◽

Conservation Plans ◽

High Degree

Abstract. The state of the local breeds of farm animals is increasingly precarious worldwide because of the aggressive introduction of breeds with improved economical traits. The preference of the breeders for local breeds is due to their higher adaptability to the particular climate and relief conditions of the mountain areas, to the high rate of assimilation of the feeds from these regions and to their increased resistance to diseases. This study analyzes the genetic variation of the main four local Romanian sheep breeds (Tsurcana, Tsigai, Ratska and Teleorman Blackhead) in terms of stock and economic importance, using 18 microsatellite markers. The mean number of alleles per locus was of 9.764. The values of genetic diversity parameters exhibited a high degree of polymorphism for the analyzed breeds, although inbreeding was highlighted particularly in Tsurcana and Tsigai. These breeds also showed an intense gene flow among them and were less differentiated in comparison with Ratska and Teleorman Blackhead. The results of this study may be useful for breeding programs and conservation plans since the genetic resources of the local breeds must be preserved so as to maintain an adequate level of biodiversity in animal husbandry.

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Genetic diversity and relationships of Chinese donkeys using microsatellite markers

Archives Animal Breeding ◽

10.5194/aab-62-181-2019 ◽

2019 ◽

Vol 62 (1) ◽

pp. 181-187 ◽

Cited By ~ 2

Author(s):

Lulan Zeng ◽

Ruihua Dang ◽

Hong Dong ◽

Fangyu Li ◽

Hong Chen ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Relationships ◽

Mean Value ◽

Principal Coordinates Analysis ◽

Expected Heterozygosity ◽

Principal Coordinates ◽

Baseline Information ◽

Grouping Pattern ◽

The Mean

Abstract. Donkeys are one important livestock in China because of their nourishment and medical values. To investigate the genetic diversity and phylogenetic relationships of Chinese donkey breeds, a panel of 25 fluorescently labeled microsatellite markers was applied to genotype 504 animals from 12 Chinese donkey breeds. A total of 226 alleles were detected, and the expected heterozygosity ranged from 0.6315 (Guanzhong) to 0.6999 (Jiami). The mean value of the polymorphism information content, observed number of alleles, and expected number of alleles for all the tested Chinese donkeys were 0.6600, 6.890, and 3.700, respectively, suggesting that Chinese indigenous donkeys have relatively abundant genetic diversity. Although there were abundant genetic variations found, the genetic differentiation between the Chinese donkey breeds was relatively low, which displayed only 5.99 % of the total genetic variance among different breeds. The principal coordinates analysis clearly splits 12 donkey breeds into two major groups. The first group included Xiji, Xinjiang, Liangzhou, Kulun, and Guanzhong donkey breeds. In the other group, Gunsha, Dezhou, Biyang, Taihang, Jiami, Qingyang, and Qinghai donkeys were clustered together. This grouping pattern was further supported by structure analysis and neighbor-joining tree analysis. Furthermore, genetic relationships between different donkey breeds identified in this study were corresponded to their geographic distribution and breeding history. Our results provide comprehensive and precise baseline information for further research on preservation and utilization of Chinese domestic donkeys.

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