scholarly journals Phytochemical and biological activities evaluation of latex from Himatanthus obovatus (Muell. Arg.) Woodson (Apocynaceae)

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
João Vitor Souza Ramada ◽  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinicius Abadia Ventura ◽  
Hellen Regina Fernandes Batista-Ventura ◽  
Celiana Maria Ferrarini Triches ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Hemolytic Activity ◽  
Biological Activities ◽  
Folk Medicine ◽  
Artemia Salina ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
E Coli

Himatanthus obovatus is a tree species that has laticifers in its aerial organs, where it is used in folk medicine. The aim of this work was to evaluate the latex of H. obovatus regarding its phytochemical composition and biological activities. The latex was collected and lyophilized, yield (%) was carried out, phytochemical prospection for several groups, determination of the content of flavonoids, phenolics and total tannins, antioxidant activity in reducing DPPH, antifungal activity on the Candida genus, antibacterial for Escherichia coli, Staphylococcus aureus, Salmonella serovar Enteritidis and Thyphymurium and Enterococcus faecalis, hemolytic activity on human erythrocytes and cytotoxicity bioassay on Artemia salina. The latex yield was 19.05%, positive presence of several groups of phytocompounds was observed, total flavonoid content of 78.80 mg QE 100 g-1, total phenolic compounds of 166.51 mg AGE g 100-1, total tannins of 14.81 mg TAE 100 g-1, antioxidant activity IC50 = 178.87 µg mL-1, antifungal activity for all Candida strains and antibacterial only for E. coli and E. faecalis, hemolytic activity between 68-18% and cytotoxicity with LC50 = 433.97 µg mL-1 on A. salina. The latex of Himatanthus obovatus demonstrated in this study, important phytochemical data and biological activities.

scholarly journals DETERMINATION OF THE QUALITY CHARACTERISTICS OF NATURALLY GROWING HAWTHORN IN SUŞEHRI

2020 ◽  
Vol 19 (1) ◽  
pp. 61-70
Author(s):  
Erdal Ağlar ◽  
Ahmet Sümbül ◽  
Orhan Karakaya ◽  
Burhan Ozturk
Keyword(s):  
Antioxidant Activity ◽  
Fruit Weight ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Fruit Characteristics ◽  
Ph Values ◽  
Biochemical Measurements

The study was conducted in 2017 in the district of Sivas. In the study, 20 genotypes, which are considered to be different from each other, taking into account the fruit characteristics such as color and size and shape, were determined from the hawthorns that were naturally grown in the flora of Suşheri. At harvest time, the fruit, which would be adequate for pomological and biochemical measurements and analyzes, was harvested. According to the results of the measurements and analyzes in the study, fruit weight was found to vary between 0.68 g and 6.35 g, fruit width was between 10.52 and 29.48 mm and fruit length was between 11.40 and 20.67 mm. The highest firmness values were recorded with the genotype (G) 20 genotype, while the G4 had the lowest values in terms of the firmness values of the fruit flesh. While there are no significant differences between the pH values of the genotypes, the differences between the genotypes in terms of SSC, TA and vitamin C contents are quite significant. It has been found that there are significant differences between the genotypes in terms of total phenolic, total flavonoid and antioxidant activity. The total phenolic content ranged from 218.8 (G17) to 605.8 (G5 and G8) mg GAE kg–1 f.w., while the lowest total flavonoid content was 21.58 (G 17) and the highest total flavonoid content was 67.75 (G9) mg GAE kg–1 f.w. When the antioxidant activity was evaluated, the DPPH values were 1.08 (G17) – 15.43 (14) mmol TE kg–1 f.w., the FRAP values were 15.43 (G16) – 47.23 (G8) mmol TE kg–1 f.w. respectively.

scholarly journals Determination of Biological Activities of the Root Bark of Senna singueana

2020 ◽  
pp. 25-34
Author(s):  
Kidanemariam Teklay Hilawea ◽  
Zelalem Yibralign Desta
Keyword(s):  
Antibacterial Activity ◽  
Biological Activities ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aims: Senna singueana is a medicinal plant which is used for the treatment of different kinds of diseases and the plant was selected for the study because of its numerous uses. The main objective of this study was to determine the antioxidant and antibacterial activity of the extracts of the root barks of S. singueana. Methodology: The antibacterial activities of the extracts (determination of Minimal Inhibitory “MIC” and Minimal Bactericidal Concentration “MBC”) were determined by using agar well diffusion method. In addition to this the total flavonoid and total phenolic contents were determined by using aluminum chloride colorimetric complex assay and Folin-Ciocalteu method respectively. Results: Our results revealed that the total flavonoid content of the extracts is ranged from 30.39 mgQE/100 g to 240.83 mgQE/100 g. The extracts also showed good antioxidant activity and total phenolic content as well as weak to moderate antibacterial activity against some bacteria. Conclusions: The extracts of the root bark of Senna sinueana showed good total flavonoid content, DPPH radical scavenging activity and antibacterial activity. In addition to this, the extracts also showed the presence of some important compounds by phytochemical analysis.

Antioxidant and antifungal activities of Myrianthus arboreus P. Beauv. (Moraceae), Allanblackia gabonensis Pellegr. (Clusiaceae) and three other Cameroonian medicinal plants

2021 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
Aimé G. Fankam ◽  
Serge S. Atsafack ◽  
Guy S. S. Njateng ◽  
Jules R. Kuiate
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Medicinal Plants ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Bark Extract ◽  
Total Phenolic ◽  
Antifungal Activities ◽  
Allanblackia Gabonensis

Background: Biological activities of medicinal plants make them attractive alternative complementary medicines. Therefore, this study aimed to assess the antioxidant and antifungal activities of the methanol extract of Myrianthus arboreus, Allanblackia gabonensis, Gladiolus quartinianus, Peperomia fernandopoiana and Vepris soyauxii. Methods: Total phenolic contents (TPC) and flavonoid contents (FC) were evaluated using standard methods. The antioxidant activity of the extracts was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing power assays. Antifungal activity was evaluated by the broth micro-dilution method. Results: TPC of extracts ranging from 67.89 to 196.01 mgGAE/g TPC were very higher in P. fernandopoiana whole plant (PFW) extract (196.01±10.25 mgGAE/g) and MAL (165.71±10.68 mgGAE/g) whereas the A. gabonensis fruit extract (AGF) and M. arboreus bark extract (MAB) had the highest flavonoid content (66.54 ± 2.12 and 48.13 ± 1.65 mgEC/g of extract, respectively). Apart from M. arboreus leave extract (MAL), all extracts showed important DPPH radical scavenging activity (IC50 < 20 μg/mL). AGFl and PFW exhibited significant ferric reducing power compared to ascorbic acid and other extracts (P<0.05). Each of the tested extracts showed antifungal activity against atleast one of the five pathogenic fungi, with MICs ranging from 128 to 1024 µg/mL. MAL was active on all the tested fungi whereas MAB showed the highest antifungal activity (MIC=128 µg/mL). Conclusion: The findings of this study emphasize the evidence that the methanol extracts of the leave and bark of Myrianthus arboreus could be an alternative to fight against oxidative stresses and infections due to opportunistic yeast pathogens. Keywords: Oxidative stress; fungal infection; medicinal plant; antioxidant activity; antifungal activity.

scholarly journals Determination of Antioxidant Activity of Selected Vegetables Grown in Iran under Reversed Phase Conditions with UPLC

2015 ◽  
Vol 9 (5) ◽  
pp. 122-125
Author(s):  
Nahid Rastakhiz ◽  
Fereshteh Khosravi ◽  
Seyyed Sina Seyyed Jafari Olia ◽  
Behzad Iranmanesh
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Reversed Phase ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reverse Phase

This study describes the determination of some phenolic compounds in four different vegetables of Kerman in Iran including Carrots, Celery, Lettuce and red cabbage .The  phenolic compounds analyzed were (Ascorbate, Ferulic acid, Naringin) using reverse phase high performance liquid chromatography (RPUPLC). The results of analysis showed in vegetables ranged between 90 and 1080mg /kg. A high and significant correlation between antioxidant activity and total phenolic content was determined in vegetables (r2 = 0.9461 P < 0.06). However, flavonoid content was not significantly correlated with antioxidant activity in vegetables. It was observed that total phenolic content is the major contributor to the antioxidant activity of vegetables.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12712

scholarly journals Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Souad Senhaji ◽  
Fatima Lamchouri ◽  
Hamid Toufik
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Endemic Plant

This study aims to investigate the biological activities of endemic plant Anabasis aretioïdes harvested in eastern Morocco. Various extracts were prepared from aerial part by aqueous and organic extraction using a Soxhlet and cold maceration. Preliminary phytochemical analysis was carried out on the powder and on the different extracts by standard phytochemical tests, and was confirmed by a quantitative analysis based on the determination of total polyphenols and cathechic tannins. Antioxidant activity was evaluated in vitro by five methods: H2O2, DPPH, ABTS, FRAP, and RP, and the antibacterial activity was carried out by disc diffusion method and the determination of MIC and MBC. Phytochemical screening revealed the presence of cathechic tannins, saponins, and sterols and quantitative analysis showed that Ethyl acetate extract presented the high level of phenolic and cathechic tannins contents (46.79 ± 0.75 µg GAE/mg E and 46.46 ± 0.67 µg CE/mg E). A highest hydrogen peroxide activity was observed in aqueous macerated extract (7.84 ± 0.44%) and the macerated methanol extract has the highest rates for the other four antioxidant activity tests: It was able to reduce DPPH with an IC50 of 52.91 ± 0.24 µg/ml, the highest ABTS•+ radical scavenging capacity (48.99 ± 1.316 µg TE/mg E), it showed also the highest antioxidant activity by the FRAP and reducing power test (99.73 ± 3.570 µg TE/mg E and 72.176 ± 0.540 µg AAE/mg E). Antibacterial screening showed that the maximum zone of inhibition was noted for ethyl acetate extract against Staphylococcus aureus (13.5 mm). The lowest MIC value was obtained with methanolic and macerated methanolic extracts against Protéus mirabilis strain (MIC = 3.125 mg/ml). Principal component analysis showed that the four methods ABTS, DPPH, FRAP, and RP are highly correlated and a correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant.

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