DEVELOPMENT OF A METHOD FOR CONSTRUCTING AND ASSESSING THE RELIABILITY OF HYPOTHESES ABOUT THE INTERRELATION OF A COMBINATION OF CYTOKINES AND OPHTHALMIC PREPARATIONS USING AN INTELLIGENT INFORMATION SYSTEM

2021 ◽  
pp. 62-70
Author(s):  
Андрей Александрович Давыденко ◽  
Ирина Владимировна Баранникова ◽  
Виталий Вячеславович Челноков ◽  
Леонид Иванович Руссу ◽  
Марина Владимировна Мезенцева
Keyword(s):  
Information System ◽  
Inductive Inference ◽  
Cell Structure ◽  
Simultaneous Action ◽  
Ophthalmic Drugs ◽  
Subjective Factor ◽  
Intelligent Information ◽  
The Relationship ◽  
Number Of Cells

В работе рассмотрено сравнение экспериментальных распределений количества клеток для определения цитологического и иммунологического эффекта, оказываемого одновременным воздействием офтальмологических препаратов Броксинак® и Офтальмоферон® на клетки с конъюнктивы больного человека, направленное на совершенствование методики оценки комбинаций офтальмологических лекарственных средств in vitro. Доклинические исследования дают возможность определить эффективность применения лекарственных средств, возможные противопоказания и побочные эффекты, чтобы в дальнейшем определиться с объемом клинических испытаний и самой возможностью их проведения. В последнее время в различных областях медицины применяют счетчики клеток. Счетчики определяют не только количество клеток в образце, но и их размеры и объем, что позволяет сделать предположение о виде клеточной структуры. Подобные приборы позволяют автоматизировать подсчет клеток, определение их размеров и объема, что исключает субъективный фактор и возможность получения ошибочных данных, а также упрощает процедуру и позволяет получить результаты в короткий срок. На основе использования методов индуктивного вывода предложена методика построения гипотез о взаимосвязи комбинации цитокинов с пролиферативной активностью клеток. Методика учитывает синергическое взаимодействие цитокинов и использует последовательное построение логических формул для отбора групп цитокинов, статистический анализ таблиц сопряженности и логическую интеграцию полученных оценок. Реализация предложенной методики в рамках ИС позволит существенно ускорить научные исследования в этой области. Приведена итоговая методика построения гипотез о взаимосвязи комбинации цитокинов с биологической активностью клеток The paper considers a comparison of experimental distributions of the number of cells to determine the cytological and immunological effect of the simultaneous action of ophthalmic drugs Broxinac® and Oftalmoferon® on cells from the conjunctiva of a sick person, aimed at improving the methodology for assessing combinations of ophthalmic drugs in vitro. Preclinical studies make it possible to determine the effectiveness of the use of drugs, possible contraindications and side effects, in order to further determine the volume of clinical trials and the very possibility of their conduct. Recently, cell counters have been used in various fields of medicine. Counters determine not only the number of cells in the sample, but also their size and volume, which allows us to make an assumption about the type of cell structure. Such devices allow automating the counting of cells, determining their size and volume, which eliminates the subjective factor and the possibility of obtaining erroneous data, and also simplifies the procedure and allows you to get results in a short time. Based on the use of inductive inference methods, a method is proposed for constructing hypotheses about the relationship between the combination of cytokines and the proliferative activity of cells. The method takes into account the synergistic interaction of cytokines and uses the sequential construction of logical formulas for the selection of groups of cytokines, statistical analysis of contingency tables and logical integration of the estimates obtained. The implementation of the proposed methodology within the Information System will significantly accelerate research in this area. The final technique for constructing hypotheses about the relationship of the combination of cytokines with the biological activity of cells is presented

The relationship between milk yield, composition and tissue damage in a case of subclinical mastitis

1963 ◽  
Vol 30 (1) ◽  
pp. 23-33 ◽  
Author(s):  
R. Waite ◽  
P. S. Blackburn
Keyword(s):  
Chemical Composition ◽  
Milk Yield ◽  
Tissue Damage ◽  
Subclinical Mastitis ◽  
Bacteriological Examination ◽  
Cell Content ◽  
Upper Level ◽  
The Relationship ◽  
Number Of Cells

SummaryA chemical, cytological and bacteriological examination of the milk from each quarter of a cow suffering from subclinical mastitis, but giving 5–6 gal of milk per day, was made at weekly intervals during the first 84 days after parturition. The infection, caused by micrococcal and staphylococcal bacteria, resisted repeated treatments with various antibiotics administered via the teat canals and also intramuscularly, although in vitro the bacteria were susceptible to all the antibiotics used. The cow was slaughtered and a histological examination made of the udder in an attempt to establish the cause of the continuing infection and to assess the extent of tissue damage. Two quarters each contained large abscesses in the upper level of the udder and these could have acted as reservoirs of infection; no cause was established for the other two quarters and it can only be assumed that reinfection occurred from the two abscessed quarters.The amount of active lesions in the lobules of all the quarters was small, 1–6%, but half or more of all the lobules were involuted, although only a minority appeared to have involuted as a result of infection. In the two abscessed quarters there was extensive damage to the duct system, 23 and 31% showing lesions.When the milk contained an abnormally large number of cells the chemical composition was also abnormal, containing less lactose (and hence less solids-not-fat) and having a nitrogen distribution in which there was more blood serum albumin and globulin and less casein than usual. Cell content and chemical composition were better indicators of tissue damage than the presence of mastitis organisms. It is estimated that the solids-not-fat content of the milk of the whole udder as a result of the infections was considerably lower than it would otherwise have been (8·0 instead of 8·8%) and that the daily loss in milk yield was about 9 lb.

scholarly journals THE PROGNOSTIC MODEL OF ANTITUMOR EFFECT OF TARGETED DRUGS IN IMMUNOTHERAPY

2016 ◽  
Vol 11 (4) ◽  
pp. 69-74 ◽  
Author(s):  
V. V. Burlyaev ◽  
А. А. Davydenko ◽  
O. M. Nikolaeva ◽  
L. I. Russu ◽  
I. A. Suetina ◽  
...  
Keyword(s):  
Information System ◽  
Statistical Analysis ◽  
Proliferative Activity ◽  
Prognostic Model ◽  
Antitumor Effect ◽  
Inductive Inference ◽  
Targeted Drugs ◽  
Inference Methods ◽  
Relationship Of ◽  
The Relationship

A prognostic model for constructing hypotheses about the relationship of combinations of cytokines with the proliferative activity of cancer cells is proposed. The model is based on the use of inductive inference methods. The methodology takes into account the synergistic interaction of cytokines and uses sequential construction of logical formulas for selecting groups of cytokines, a statistical analysis of contingency tables and logical integration of the obtained estimates. Implementation of the proposed method in the information system of forecasting the effect of targeted anticancer drugs in immunotherapy will greatly accelerate research in this area.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS

1963 ◽  
Vol 17 (2) ◽  
pp. 423-441 ◽  
Author(s):  
Nicole Granboulan ◽  
P. Tournier ◽  
R. Wicker ◽  
W. Bernhard
Keyword(s):  
Electron Microscope ◽  
Close Contact ◽  
Kidney Cells ◽  
Thin Sections ◽  
Sv40 Virus ◽  
Virus Particles ◽  
The Relationship ◽  
Infectious Cycle ◽  
Number Of Cells

Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus. The infectious cycle was studied with the electron microscope by means of thin sections of cells fixed from 3 hours up to 11 days after infection. The frequency of virus formation and various nuclear and cytoplasmic lesions in relation to the infection are described. The virus particles appear in the nucleus in close contact with the chromatin. In a small number of cells they have been observed as early as 10 to 12 hours after infection, but most often they appear 24 to 48 hours afterward. Their mean diameter is 33 mµ. They have no membrane and are frequently arranged as crystal-like structures. In addition to the appearance of virus, one observes various lesions in the nucleoplasm and particularly in the nucleolus, which shows an early hypertrophy and produces unusual, dense condensations in contact with the nucleolonema. The importance of these nucleolar lesions and the relationship between the SV40 virus and the polyoma, common wart, and Shope papilloma viruses are discussed.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Plasminogen Depletion during Streptokinase Treatment or Two-Chain Urokinase Incubation Correlates with Decreased Clot Lysability Ex Vivo and In Vitro

1993 ◽  
Vol 70 (06) ◽  
pp. 0998-1004 ◽  
Author(s):  
Páll T Önundarson ◽  
H Magnús Haraldsson ◽  
Lena Bergmann ◽  
Charles W Francis ◽  
Victor J Marder
Keyword(s):  
Myocardial Infarction ◽  
Ex Vivo ◽  
Time Dependent ◽  
State Variables ◽  
Thrombolytic Treatment ◽  
Direct Proportion ◽  
Plasmin Activity ◽  
Plasma Exposure ◽  
The Relationship

SummaryThe relationship between lytic state variables and ex vivo clot lysability was investigated in blood drawn from patients during streptokinase administration for acute myocardial infarction. A lytic state was already evident after 5 min of treatment and after 20 min the plasminogen concentration had decreased to 24%, antiplasmin to 7% and fibrinogen 0.2 g/1. Lysis of radiolabeled retracted clots in the patient plasmas decreased from 37 ± 8% after 5 min to 21 ± 8% at 10 min and was significantly lower (8 ± 9%, p <0.005) in samples drawn at 20, 40 and 80 min. Clot lysability correlated positively with the plasminogen concentration (r = 0.78, p = 0.003), but not with plasmin activity. Suspension of radiolabeled clots in normal plasma pre-exposed to 250 U/ml two-chain urokinase for varying time to induce an in vitro lytic state was also associated with decreasing clot lysability in direct proportion with the duration of prior plasma exposure to urokinase. The decreased lysability correlated with the time-dependent reduction in plasminogen concentration (r = 0.88, p <0.0005). Thus, clot lysability decreases in conjunction with the development of the lytic state and the associated plasminogen depletion. The lytic state may therefore limit reperfusion during thrombolytic treatment.

Monitoring Anticoagulant Therapy by Activated Partial Thromboplastin Time: Hirudin Assessment

1994 ◽  
Vol 72 (05) ◽  
pp. 685-692 ◽  
Author(s):  
Michael T Nurmohamed ◽  
René J Berckmans ◽  
Willy M Morriën-Salomons ◽  
Fenny Berends ◽  
Daan W Hommes ◽  
...  
Keyword(s):  
Whole Blood ◽  
Partial Thromboplastin Time ◽  
Ex Vivo ◽  
Fold Increase ◽  
Heparin Therapy ◽  
Activated Partial Thromboplastin Time ◽  
Recombinant Hirudin ◽  
Interindividual Differences ◽  
The Relationship

SummaryBackground. Recombinant hirudin (RH) is a new anticoagulant for prophylaxis and treatment of venous and arterial thrombosis. To which extent the activated partial thromboplastin time (APTT) is suitable for monitoring of RH has not been properly evaluated. Recently, a capillary whole blood device was developed for bed-side monitoring of the APTT and it was demonstrated that this device was suitable to monitor heparin therapy. However, monitoring of RH was not evaluated.Study Objectives. To evaluate in vitro and ex vivo the responsiveness and reproducibility for hirudin monitoring of the whole blood monitor and of plasma APTT assays, which were performed with several reagents and two conventional coagulometers.Results. Large interindividual differences in hirudin responsiveness were noted in both the in vitro and the ex vivo experiments. The relationship between the APTT, expressed as clotting time or ratio of initial and prolonged APTT, and the hirudin concentration was nonlinear. A 1.5-fold increase of the clotting times was obtained at 150-200 ng/ml plasma. However, only a 2-fold increase was obtained at hirudin levels varying from 300 ng to more than 750 ng RH/ml plasma regardless of the assays. The relationship linearized upon logarithmic conversion of the ratio and the hirudin concentration. Disregarding the interindividual differences, and presuming full linearity of the relationship, all combinations were equally responsive to hirudin.Conclusions. All assays were equally responsive to hirudin. Levels up to 300 ng/ml plasma can be reliably estimated with each assay. The manual device may be preferable in situations where rapid availability of test results is necessary.

FORMALIZED DESCRIPTION OF THE PROCEDURE OF INFORMATION SYSTEM RISK MANAGEMENT

2018 ◽  
pp. 61-70
Author(s):  
Svetlana Sergeevna Kozunova ◽  
Alla Grigorievna Kravets
Keyword(s):  
Information System ◽  
Risk Management ◽  
Negative Consequences ◽  
Management Procedure ◽  
System Risk ◽  
Weak Points ◽  
Formalized Description ◽  
Relationship Of ◽  
The Relationship ◽  
Further Development

The article highlights the aspects of risk management in the information system. According to the analysis of the work of Russian and foreign scientists and world practices in the field of risk management, it is stated that there is a need to improve the effectiveness of risk management of information system and to develop a method for managing the risks of the information system. As a solution to the problem of effective risk management of the information system, there has been proposed a formalized procedure for managing the risks of the information system. The scientific novelty of this solution is the use of decision space and optimization space to reduce risks. This procedure allows to assess the damage, risk and effectiveness of risk management of the information system. The risks of the information system are determined and analyzed; a pyramidal risk diagram is developed. This diagram allows you to describe the relationship of risks with the components of the information system. The negative consequences to which these risks can lead are given. The analysis of methods and approaches to risk management has been carried out. Based on the results of the analysis, the methods GRAMM, CORAS, GOST R ISO / IEC scored to the maximum. The weak points of these methods and the difficulty of applying these methods in practice are described. The developed formalized risk management procedure to control the risks of information system can be used as management system’s element of the information security quality that complies with the recommendations of GOST R ISO / IEC 27003-2012. The prospect of further development of the research results is the development of management systems of risk of information system.

Thickness of endometrium: predictor of the effectiveness of IVF/ICSI programs (literature review)

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 113-116
Author(s):  
L A Bagdasaryan ◽  
I E Korneyeva
Keyword(s):  
Assisted Reproductive Technologies ◽  
Endometrial Thickness ◽  
Molecular Genetic ◽  
Uterine Cavity ◽  
Reproductive Technologies ◽  
Genetic Characteristics ◽  
Vitro Fertilization ◽  
Need To Evaluate ◽  
The Relationship

The aim of the study is to systematically analyze the data available in the modern literature on the relationship between endometrial thickness and the frequency of pregnancy in the program of assisted reproductive technologies (ART). Materials and methods. The review includes data from foreign and domestic articles found in PubMed on this topic. Results. The article presents data on the relationship between the thickness of the endometrium and the frequency of pregnancy in ART programs. The greatest number of studies is devoted to the evaluation of the relationship between the thickness of the endometrium and the frequency of pregnancy on the day of the ovulation trigger. Data are presented on the existence of a correlation between the thickness of the endometrium measured on the day of the ovulation trigger and the frequency of clinical pregnancy, as well as data on the need to evaluate the structure of the endometrium and the state of subendometric blood flow. The importance of multilayered (three-layered) endometrium as a prognostic marker of success in in vitro fertilization/intracytoplasmic sperm injection programs in the ovum is emphasized. The conclusion. The thickness of the endometrium can not be used as an argument for canceling the cycle or abolishing embryo transfer to the uterine cavity. Further studies in this direction are needed with a study of the morphological and molecular genetic characteristics of the endometrium, which in the future will allow us to evaluate the relationship between the thickness of the endometrium and the probability of pregnancy.

Structural Mediation Model of Information System and Sustainable Performance of UAE Manufacturing Sector

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Mohamed Ahmed Darwish Abdulla Larii ◽  
◽  
Fatma Ahmed Lari ◽  
Mohamed Ahmed Darwish Abdulla Lari ◽  
◽  
...  
Keyword(s):  
Information System ◽  
Manufacturing Sector ◽  
Organisational Culture ◽  
The Body ◽  
Mediating Effect ◽  
Operational Performance ◽  
Manufacturing Companies ◽  
Mediation Model ◽  
Sustainable Performance ◽  
The Relationship

This study intends to find out the mediating effect of organisational culture on the relationship between information system and sustainable performance of manufacturing sector in UAE. This study used AMOS-SEM software to develop mediation model that linking the mediating relationships between Information System, Organisational Culture and Sustainable operation Performance. Data was collected through questionnaire survey among the operation staff of Abu Dhabi manufacturing companies. A total 250 questionnaires were distributed however 205 were returned and only 200 are valid which indicates a response rate of 80%. The analysis found that TPS has positive but not significant effect to SP; OIS has positive but not significant effect to SP; FMW has a positive and significant effect on SP; SDS has a negative and not significant effect to SP and SP has positive but not significant effect OC. For the path relationship between the four exogenous variables (TPS, OIS, SDS, and FMW) and the mediator variable (OC), the results are TPS has positive and significant effect to OC; OIS has positive but not significant effect to OC; FMW has positive and significant effect to OC and SDS has positive and not significant effect to OC. Collectively, the five exogenous constructs (TPS, OIS, SDS, FMW and OC) explained 89% variation in operational performance and 86% of the variation in organisational culture. However, for a mediator, it was found that OC has no significant mediating effect on the relationship between TPS and SP; OC has no significant mediating effect on the relationship between OIS and SP; OC has no significant mediating effect on the relationship between SDS and SP and OC has no significant mediating effect on the relationship between FMW and SP. it can be concluded that there is a positive relationship between information system dimensions and operational performance. However organizational culture has no contributing any mediating effect to the relationship. These findings have contributed to the body of knowledge and could be shared among the UAE manufacturing practitioners.

Sign in / Sign up

Export Citation Format

Share Document