scholarly journals Crossreactive allergen-specific IgE in children with birch pollen allergic rhinitis

2017 ◽  
Vol 14 (2) ◽  
pp. 66-70
Author(s):  
P V Samoylikov ◽  
S A Mazurina ◽  
P I Gushchin ◽  
V B Gervazieva
Keyword(s):  
Allergic Rhinitis ◽  
Birch Pollen ◽  
Humoral Response ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Oral Allergy Syndrome ◽  
Food Allergens ◽  
Homologous Proteins ◽  
Total Ige ◽  
Bet V 1

Objective. The aim of this study was to research a sIgE allergen profile of birch pollen and to evaluate a contribution of some homologous food allergens as well as latex allergen to the development of sensibility in allergic rhinitis (AR) / rhinoconjunctivitis patients, in focus of cross-reactivity and oral allergy syndrome.. Methods. Blood sera of 21 AR/rhinoconjunctivitis patients (at the age of 3 to 16) and 20 healthy persons without allergy symptoms were used. sIgE to birch pollen, soybean, latex, apple and celery as well as the total IgE levels were measured by the ImmunoCAP method (Phadia, Sweden) and the ELISA kits (Alkorbio, Russia). Results. We detected high total IgE levels, sIgE to allergens of birch pollen, apple, celery, as well as to recombinant allergens of birch Bet v 1, Bet v 2 and soybean - Gly m 4 in AR patients. Correlation analysis of IgE humoral response to homologous proteins showed the direct valid dependence between the sIgE levels to birch isoallergen Bet v 1 and soy isoallergen Gly m 4 (r=0,84; p

scholarly journals Cross-reactivity of pollen and food allergens: soybean Gly m 4 is a member of the Bet v 1 superfamily and closely resembles yellow lupine proteins

2009 ◽  
Vol 29 (3) ◽  
pp. 183-192 ◽  
Author(s):  
Hanna Berkner ◽  
Philipp Neudecker ◽  
Diana Mittag ◽  
Barbara K. Ballmer-Weber ◽  
Kristian Schweimer ◽  
...  
Keyword(s):  
Physiological Function ◽  
Three Dimensional ◽  
Birch Pollen ◽  
Cross Reactivity ◽  
Food Allergens ◽  
Homologous Proteins ◽  
Allergen Specific Immunotherapy ◽  
Ige Antibodies ◽  
Bet V 1 ◽  
Yellow Lupine

In many cases, patients allergic to birch pollen also show allergic reactions after ingestion of certain fruits or vegetables. This observation is explained at the molecular level by cross-reactivity of IgE antibodies induced by sensitization to the major birch pollen allergen Bet v 1 with homologous food allergens. As IgE antibodies recognize conformational epitopes, a precise structural characterization of the allergens involved is necessary to understand cross-reactivity and thus to develop new methods of allergen-specific immunotherapy for allergic patients. Here, we report the three-dimensional solution structure of the soybean allergen Gly m 4, a member of the superfamily of Bet v 1 homologous proteins and a cross-reactant with IgE antibodies originally raised against Bet v 1 as shown by immunoblot inhibition and histamine release assays. Although the overall fold of Gly m 4 is very similar to that of Bet v 1, the three-dimensional structures of these proteins differ in detail. The Gly m 4 local structures that display those differences are also found in proteins from yellow lupine with known physiological function. The three-dimensional structure of Gly m 4 may thus shed some light on the physiological function of this subgroup of PR10 proteins (class 10 of pathogenesis-related proteins) and, in combination with immunological data, allow us to propose surface patches that might represent cross-reactive epitopes.

Bet v 1142-156 is the dominant T-cell epitope of the major birch pollen allergen and important for cross-reactivity with Bet v 1–related food allergens

2005 ◽  
Vol 116 (1) ◽  
pp. 213-219 ◽  
Author(s):  
Beatrice Jahn-Schmid ◽  
Astrid Radakovics ◽  
Dirk Lüttkopf ◽  
Stephan Scheurer ◽  
Stefan Vieths ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Epitope ◽  
Birch Pollen ◽  
Cross Reactivity ◽  
Pollen Allergen ◽  
T Cell Epitope ◽  
Food Allergens ◽  
Bet V 1 ◽  
Birch Pollen Allergen

Birch pollen associated soy allergy – possibilities in diagnostic and clinical relevance1)

2012 ◽  
Vol 36 (3) ◽  
Author(s):  
Susanne Beyer ◽  
Ulrich Sack ◽  
Regina Treudler
Keyword(s):  
Birch Pollen ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Oral Allergy Syndrome ◽  
Food Allergies ◽  
Food Allergens ◽  
Prick Test ◽  
The Past ◽  
Immunological Cross Reaction

AbstractBirch pollen allergic individuals frequently suffer from food allergies in the form of an oral allergy syndrome after eating pome and stone fruits. These complaints are based on an immunological cross-reaction between pollen and food allergens. In the past, it has been shown that many birch pollen allergic patients are additionally not able to tolerate high protein soy products. Some severe immediate type reactions to soy have been observed. The cause for these immediate type reactions to soy is a Bet v 1 cross-reactive soy allergen called Gly m 4.Using a collective of 73 birch pollen allergic patients with associated food allergy in Leipzig as an example, the results of a standardized questioning, prick-to-prick test with a soy drink, determination of specific IgE against rGly m 4, and basophil activation test with Gly m 4 are presented.We showed that commercially available prick test extracts and determination of specific IgE against soy bean mix/f14 are not appropriate to diagnose birch pollen associated soy allergy. Generally, soy sensitization could be proven when a prick-to-prick-test with a soy drink and determination of specific IgE against rGly m 4 were done. A positive prick-to-prick test with a soy drink was found in 79% (55/70) of the birch pollen allergic patients with 89% (65/73) showing specific IgE for rGly m 4 (CAP>1). Although not every sensitization was clinically relevant, every third patient with a proven soy sensitization was diagnosed with a clinically relevant allergy to soy.

scholarly journals Oral allergy syndrome in children with pollen sensitization in Tomsk region

2016 ◽  
Vol 13 (6) ◽  
pp. 58-62
Author(s):  
M M Fedotova ◽  
O S Fedorova ◽  
L M Ogorodova ◽  
T A Evdokimova
Keyword(s):  
Skin Prick Testing ◽  
Clinical Stage ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Cross Reactivity ◽  
Oral Allergy Syndrome ◽  
Pollen Allergens ◽  
Cross Sectional ◽  
Bet V 1 ◽  
Tomsk Region

Background. Oral allergy syndrome (OAS) is defined as immediate IgE-mediated allergic reaction localized in the oral mucosa and developing after consuming raw fruits, vegetables, nuts, legumes in pollen sensitized patients. Objective. To study the prevalence of OAS in children in the Tomsk region. Material. The cross-sectional study in random groups of primary schoolchildren aged 7-10 years (n=13 010) from the Tomsk region, Russia, was performed in frames of (EuroPrevall, № FP6-2006-TTC-TU-5 Proposal 045879). During the screening phase a survey with standardized questionnaire was carried out. Clinical stage (n=1288) included clinical interviewing with parents/guardians, the clinical examination of patients, skin prick testing with extracts of food and pollen allergens (ALK-Abello, Spain), measurement of specific IgE level in serum to food and pollen allergens, component resolved diagnostics (ImmunoCAP, Phadia, Sweden). Results. OAS registered in 13,71% of children with pollen sensitization. The main triggers were apples, carrots, peaches, peanuts. Main cause of OAS in the Tomsk region was cross-reactivity to Bet v 1 - homologues belonging to PR-10 family: to apple - Mal d 1 (r=0,92; p=0,01); to peach - Pru p1 (r=0,87; p=0,01); to peanut - Ara h 8 (r=0,74; p=0,01); to hazelnut - Cor a 1 (r=0,76; p=0,01); to carrot - Dau c 1 (r=0,54; p=0,01). Conclusion. OAS was observed in 13,71% of children with pollen sensitization and was developesed due to crossreactivity to the birch allergen Bet v 1.

scholarly journals Atopic Status in Children with Asthma and Respiratory Allergies—Comparative Analysis of Total IgE, ImmunoCAP Phadiatop/fx5 and Euroimmun Pediatric Immunoblot

Sinusitis ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1-14
Author(s):  
Snezhina Lazova ◽  
Marta Baleva ◽  
Stamatios Priftis ◽  
Emilia Naseva ◽  
Tsvetelina Velikova
Keyword(s):  
Allergic Rhinitis ◽  
Comparative Analysis ◽  
Gold Standard ◽  
Specific Ige ◽  
Food Allergens ◽  
Laboratory Methods ◽  
Total Ige ◽  
Atopic Status ◽  
Respiratory Allergies ◽  
Children With Asthma

Introduction: An atopic status assessment (skin prick test or specific immunoglobulin (sIgE)) in asthmatic children is considered a milestone in identifying potential risk factors and triggers provoking loss of asthma control and asthma exacerbation. Objective: The study aims to perform a comparative analysis of different laboratory methods for a serological assessment of an atopic status in asthma and respiratory allergies in children. Material and methods: A total of 86 children were included, all of whom were diagnosed with bronchial asthma, aged from 5 to 17 years and screened for total IgE level using enzyme-linked immunosorbent assay (ELISA). In 48 randomly selected children, we performed a semi-quantitative serological in vitro assessment of the specific IgE antibodies against food and aeroallergen, using two different laboratory methods—Euroimmun Immunoblot and ImmunoCAP (Phadiatop/fx5). Results: In 70% of the children with a history of allergies, and 65.3% without clinically manifested allergies, multiscreen test ImmunoCAP Phadiatop/fx5 showed positivity and confirmed atopy. Our results showed a significant moderate to strong correlation between multiscreen ImmunoCAP Phadiatop/fx5, and Euroimmun specific IgE titers against aero-allergens—cats, mites, tree mix and food allergens—soy, wheat (р = 0.006), rice, р = 0.090), apple р = 0.007) and peanut. A sensitivity of 63% and specificity of 73.5% was observed for EUROIMMUN Pediatric (food allergens, IgE titer > 1) compared with the gold standard ImmunoCap/fx5. The mean value of total IgE is significantly higher in children with asthma and concomitant with allergic rhinitis compared to those without allergic rhinitis (mean 202.52 U/mL, IQR 102.50 (24.20–363.95) vs. 316.68, IQR 261.00 (109.20–552.50), p = 0.005). Conclusion: Establishing the spectrum of the most common respiratory and food allergens is an essential factor for maintaining asthma control, both through a strategy to avoid allergen exposure and by developing a recommendation plan. The immunoblotting technique is easily applicable in daily clinical and laboratory practice. It is also a cost-effective and reliable alternative to the “gold standard” ImmunoCAP Phadiatop/fx5 in diagnosing atopy in children.

scholarly journals Correlated dynamics of serum IGE and IGE+ clonotype count with allergen air level in seasonal allergic rhinitis

2019 ◽  
pp. 13-22
Author(s):  
AI Mikelov ◽  
DB Staroverov ◽  
EA Komech ◽  
YuB Lebedev ◽  
DM Chudakov ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Seasonal Allergic Rhinitis ◽  
Birch Pollen ◽  
Specific Ige ◽  
Chain Gene ◽  
Initial Increase ◽  
Total Ige ◽  
Serum Ige ◽  
Time Points ◽  
Pollination Season

Mechanisms of maintenance of immunological memory in the chronic course of seasonal allergic rhinitis remain poorly understood. The detailed understanding of these mechanisms is required for design of new approaches for allergy treatment. It is known that the level of allergen-specific IgE antibodies (sIgE), which play a key role in the development of the disease, is increased in patients with seasonal allergic rhinitis during pollination season. This study aimed to investigate the dynamics of serum IgE levels and characteristics of the clonal repertoire of IgE-secreting lymphocytes depending on the intensity of the patient's contact with the allergen. For three patients, allergic to birch pollen (22, 22, and 28 y.o.), we measured total IgE and birch pollen specific IgE levels at 6 time points with 2 week interval during the birch pollination season. Immunoglobulin heavy chain gene (IGH) clonal repertoire data for several B-cell subpopulations at different time points were obtained for one patient. We observe growth of the sIgE level (91%, 37%, and 64% compared to the baseline) at the peak of pollination season in all three donors. Initial increase in sIgE and IgE levels coincides with the birch pollination initiation; sIgE and total IgE levels correlate with the birch pollen air level (sIgE: R2 = 0.98 at p < 0.05; total IgE: R2 = 0.95 at p < 0.05). We detected IgE clonotypes only in samples obtained during the birch pollination, which indicates an increase of IGE-expressing cells concentration during this period. The frequency of IgE clonotypes was extremely low compared to that of the clonotypes of other isotypes (IgE — 0.01%, IgM — 48.4%, IgD — 14%, IgG — 17.4%, IgA — 19.8%). Hypermutation and phylogenetic analysis of the sequences from the 13 detected IgE-containing clonal groups showed that these IgE clonotypes could originate from IgG as a result of sequential isotype-switching.

scholarly journals NMR resonance assignments of the PR-10 allergens Act c 8 and Act d 8 from golden and green kiwifruit

2021 ◽  
Author(s):  
Ricarda Zeindl ◽  
Martin Tollinger
Keyword(s):  
Clinical Symptoms ◽  
Chemical Shifts ◽  
Birch Pollen ◽  
Resonance Assignments ◽  
Cross Reactivity ◽  
Food Sources ◽  
Structural Basis ◽  
Homologous Proteins ◽  
Dimensional Solution ◽  
Bet V 1

AbstractKiwifruits have become one of the most common food sources triggering allergic reactions. In patients suffering from birch pollen related food allergy, reactions result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1, followed by immunological cross-reactivity to structurally homologous proteins in kiwifruit. Clinical symptoms range from scratching and itching of the oral cavity to more severe immunological reactions such as rhino conjunctivitis. In this work we assigned backbone and side chain 1H, 13C and 15N chemical shifts of the 17 kDa PR-10 allergens Act c 8.0101 and Act d 8.0101 from golden (Actinidia chinesis) and green (Actinidia deliciosa) kiwifruit by solution NMR spectroscopy. The chemical shift data confirm the characteristic Bet v 1 fold for both proteins, consisting of a seven-stranded antiparallel β-sheet interrupted by two short α-helices, along with a long C-terminal α-helix. Our data provide the basis for determining the three-dimensional solution structures of these proteins and characterizing their immunological cross-reactivity on a structural basis.

Quality and potency profile of eight recombinant isoallergens, largely mimicking total Bet v 1‐specific IgE binding of birch pollen

2019 ◽  
Vol 49 (5) ◽  
pp. 712-723 ◽  
Author(s):  
Christian Seutter von Loetzen ◽  
Andreas Reuter ◽  
Jelena Spiric ◽  
Thomas Schulenborg ◽  
Iris Bellinghausen ◽  
...  
Keyword(s):  
Birch Pollen ◽  
Specific Ige ◽  
Bet V 1 ◽  
Ige Binding

scholarly journals Sublingual Reactivity to rBET V1 and rPHL P1 in Patients with Oral Allergy Syndrome

2006 ◽  
Vol 19 (1) ◽  
pp. 205873920601900 ◽  
Author(s):  
F. Marcucci ◽  
L. Sensi ◽  
G. DI Cara ◽  
G. Gidaro ◽  
C. Incorvaia ◽  
...  
Keyword(s):  
Specific Ige ◽  
Cross Reactivity ◽  
Oral Allergy Syndrome ◽  
Control Group ◽  
Recombinant Allergens ◽  
Natural Extracts ◽  
Target Organs ◽  
High Concentrations

Oral Allergy Syndrome (OAS) in patients with pollen-induced rhinoconjunctivitis is caused by specific IgE recognizing cross-reacting epitopes of fruits and plants, which were clearly shown in vitro, but failed to be demonstrated in vivo by cross-challenges in the target organs. Considering the hypothesis of degradation of such epitopes in natural extracts, challenges with recombinant pollen allergens were done to evaluate the reactivity of the oral mucosa in OAS patients. Seventeen patients with OAS and rhinitis from birch (10) and grass pollen (7) and 10 non-atopic controls were studied by skin prick tests (SPT), allergen specific nasal challenges (ASNC) and allergen specific sublingual challenges (ASSC) with birch and timothy extracts and with rBet v1 and rPhl p1 at increasing concentrations from 1 to 1000 mcg/ml. None of the healthy subjects in the control group had any positive test for birch and timothy extracts or for recombinant allergens. In the OAS group the following results were observed: SPTs with recombinant allergens were positive in all patients, mostly at 10 mcg/ml concentration; ASNC with rBet v1 were positive in all patients, mostly at 100 mcg/ml; ASSC with natural pollen extracts were positive in only 2 of 17 patients, but in 15 of 17 with rBet v1 and rPhl p1, mostly at 500 mcg/ml and 1000 mcg/ml. ASSC with rBet v1 and rPhl p1 were positive with a mean concentration of 677 and 533 mcg/ml, respectively. The results of sublingual challenges with rBet v1 and rPhl p1 showed the in vivo cross-reactivity between pollens and foods in patients with OAS, but high concentrations of the recombinant allergens were needed to reproduce oral symptoms, thus explaining the failure of challenges performed with natural extracts, which have concentrations of major allergens lower than 50 mcg/ml. This indicates that sublingual mucosa is much less reactive to allergens than other surfaces, such as skin and nasal mucosa, probably because of its anatomic and immunologic peculiarity.

scholarly journals Agaricus blazei-Based Mushroom Extract Supplementation to Birch Allergic Blood Donors: A Randomized Clinical Trial

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2339 ◽  
Author(s):  
Faiza Mahmood ◽  
Geir Hetland ◽  
Ivo Nentwich ◽  
Mohammad Reza Mirlashari ◽  
Reza Ghiasvand ◽  
...  
Keyword(s):  
Placebo Group ◽  
Pollen Season ◽  
Blood Donors ◽  
Birch Pollen ◽  
Specific Ige ◽  
Basophil Activation ◽  
Pollen Extract ◽  
Agaricus Blazei ◽  
Oral Supplementation ◽  
Bet V 1

Since Agaricus blazei Murill (AbM) extract reduced specific IgE and ameliorated a skewed Th1/Th2 balance in a mouse allergy model, it was tested in blood donors with self-reported, IgE-positive, birch pollen allergy and/or asthma. Sixty recruited donors were randomized in a placebo-controlled, double-blinded study with pre-seasonal, 7-week, oral supplementation with the AbM-based extract AndosanTM. Before and after the pollen season, questionnaires were answered for allergic rhino-conjunctivitis, asthma, and medication; serum IgE was measured, and Bet v 1-induced basophil activation was determined by CD63 expression. The reported general allergy and asthma symptoms and medication were significantly reduced in the AbM compared to the placebo group during pollen season. During the season, there was significant reduction in specific IgE anti-Bet v 1 and anti-t3 (birch pollen extract) levels in the AbM compared with the placebo group. While the maximal allergen concentrations needed for eliciting basophil activation before the season, changed significantly in the placebo group to lower concentrations (i.e., enhanced sensitization) after the season, these concentrations remained similar in the AndosanTM AbM extract group. Hence, the prophylactic effect of oral supplementation before the season with the AbM-based AndosanTM extract on aeroallergen-induced allergy was associated with reduced specific IgE levels during the season and basophils becoming less sensitive to allergen activation.

Sign in / Sign up

Export Citation Format

Share Document