About the threshold concentration of nickel oxide nanoparticles in long-term inhalation exposure of rats

Introduction. Nickel oxide nanoparticles are of interest for toxicological science, not only as engineered nanoparticles, producing for industrial and scientific needs, but also as spontaneous pollutants of the atmosphere and the working area in industrial processes related to metallurgy and welding. Materials and methods. Rats were exposed to nickel-oxide aerosol at a concentration of 2.4 ± 0.4 µg/m3 in a “nose only” inhalation setup for 4 hours at a time, 5 times a week, during an overall period of 2 weeks to 6 months. Results. Of the several dozen examined parameters, only a few statistically significant manifestations associated with the reaction of the deep airways to inhaled nanoparticles were noted. However, in the biochemical and morphometric parameters of the lungs, even at the longest periods of exposure, the intergroup differences were insignificant. At the same time, even from the first weeks of the exposure period, genotoxic and allergic indices shifts are detected. Conclusion. For most of the evaluated effects, this level of exposure to nickel oxide nanoparticles may be considered as close to LOAEL, or even to NOAEL. However, according to some indicators, there are effects that suggest a non-threshold nature.

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Looking for the LOAEL or NOAEL Concentration of Nickel-Oxide Nanoparticles in a Long-Term Inhalation Exposure of Rats

International Journal of Molecular Sciences ◽

10.3390/ijms22010416 ◽

2021 ◽

Vol 22 (1) ◽

pp. 416

Author(s):

Boris A. Katsnelson ◽

Ivan N. Chernyshov ◽

Svetlana N. Solovyeva ◽

Ilzira A. Minigalieva ◽

Vladimir B. Gurvich ◽

...

Keyword(s):

Adverse Effect ◽

Nickel Oxide ◽

Inhalation Exposure ◽

Oxide Nanoparticles ◽

Nickel Oxide Nanoparticles ◽

Adverse Effect Level ◽

Effect Level

Rats were exposed to nickel oxide nano-aerosol at a concentration of 2.4 ± 0.4 µg/m3 in a “nose only” inhalation setup for 4 h at a time, 5 times a week, during an overall period of 2 weeks to 6 months. Based on the majority of the effects assessed, this kind of exposure may be considered as close to LOAEL (lowest observed adverse effect level), or even to NOAEL (no observed adverse effect level). At the same time, the experiment revealed genotoxic and allergic effects as early as in the first weeks of exposure, suggesting that these effects may have no threshold at all.

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Long-term effects of nickel oxide nanoparticles on performance, microbial enzymatic activity, and microbial community of a sequencing batch reactor

Chemosphere ◽

10.1016/j.chemosphere.2016.10.139 ◽

2017 ◽

Vol 169 ◽

pp. 387-395 ◽

Cited By ~ 10

Author(s):

Sen Wang ◽

Zhiwei Li ◽

Mengchun Gao ◽

Zonglian She ◽

Liang Guo ◽

...

Keyword(s):

Microbial Community ◽

Enzymatic Activity ◽

Nickel Oxide ◽

Sequencing Batch Reactor ◽

Batch Reactor ◽

Oxide Nanoparticles ◽

Long Term Effects ◽

Nickel Oxide Nanoparticles

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Comparative assessment of the effects of short-term inhalation exposure to Nickel oxide nanoparticles and microdispersed Nickel oxide

Nanotechnologies in Russia ◽

10.1134/s1995078016050219 ◽

2016 ◽

Vol 11 (9-10) ◽

pp. 671-677 ◽

Cited By ~ 3

Author(s):

N. V. Zaitseva ◽

M. A. Zemlyanova ◽

V. N. Zvezdin ◽

A. A. Dovbysh ◽

T. S. Ulanova ◽

...

Keyword(s):

Nickel Oxide ◽

Inhalation Exposure ◽

Comparative Assessment ◽

Oxide Nanoparticles ◽

Short Term ◽

Nickel Oxide Nanoparticles

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Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.

Frontiers in Plant Science ◽

10.3389/fpls.2021.586509 ◽

2021 ◽

Vol 12 ◽

Author(s):

Indrani Manna ◽

Saikat Sahoo ◽

Maumita Bandyopadhyay

Keyword(s):

Nitric Oxide ◽

Heavy Metals ◽

Reactive Oxygen Species ◽

Nickel Oxide ◽

Allium Cepa ◽

Reactive Oxygen ◽

Engineered Nanoparticles ◽

Oxide Nanoparticles ◽

Oxygen Species ◽

Nickel Oxide Nanoparticles

Scientists anxiously follow instances of heavy metals augmenting in the environment and undergoing bioaccumulation and trace their biomagnification across food webs, wary of their potent toxicity on biological entities. Engineered nanoparticles supplement natural pools of respective heavy metals and can mimic their effects, exerting toxicity at higher concentrations. Thus, a thorough understanding of the underlying mechanism of this precarious interaction is mandatory. Most urban and industrial environments contain considerable quantities of nickel oxide nanoparticles. These in excess can cause considerable damage to plant metabolism through a significant increase in cellular reactive oxygen species and perturbation of its cross-talk with the reactive nitrogen species. In the present work, the authors have demonstrated how the intrusion of nickel oxide nanoparticles (NiO-NP) affected the exposed roots of Allium cepa: starting with disruption of cell membranes, before being interiorized within cell organelles, effectively disrupting cellular homeostasis and survival. A major shift in the reactive oxygen species (ROS) and nitric oxide (NO) equanimity was also observed, unleashing major altercations in several crucial biochemical profiles. Altered antioxidant contents and upregulation of stress-responsive genes, namely, Catalase, Ascorbate peroxidase, Superoxide dismutase, and Rubisco activase, showing on average 50–250% rise across NiO-NP concentrations tested, also entailed increased cellular hydrogen peroxide contents, with tandem rise in cellular NO. Increased NO content was evinced from altered concentrations of nitric oxide synthase and nitrate reductase, along with NADPH oxidase, when compared with the negative control. Though initially showing a dose-dependent concomitant rise, a significant decrease of NO was observed at higher concentrations of NiO-NP, while cellular ROS continued to increase. Modified K/Na ratios, with increased proline concentrations and GABA contents, all hallmarks of cellular stress, correlated with ROS–NO perturbations. Detailed studies showed that NiO-NP concentration had a significant role in inducing toxicity, perturbing the fine balance of ROS–NO, which turned lethal for the cell at higher dosages of the ENP precipitating in the accumulation of stress markers and an inevitable shutdown of cellular mechanisms.

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Toxic Effects of Low-Level Long-Term Inhalation Exposures of Rats to Nickel Oxide Nanoparticles

International Journal of Molecular Sciences ◽

10.3390/ijms20071778 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1778 ◽

Cited By ~ 9

Author(s):

Sutunkova ◽

Solovyeva ◽

Minigalieva ◽

Gurvich ◽

Valamina ◽

...

Keyword(s):

Nickel Oxide ◽

Exposure Period ◽

Lavage Fluid ◽

Genotoxic Effect ◽

Oxide Nanoparticles ◽

Toxic Exposure ◽

Olfactory Pathway ◽

Nickel Oxide Nanoparticles ◽

Nio Nps

Rats were exposed to nickel oxide nanoparticles (NiO-NP) inhalation at 0.23 ± 0.01 mg/m3 for 4 h a day 5 times a week for up to 10 months. The rat organism responded to this impact with changes in cytological and some biochemical characteristics of the bronchoalveolar lavage fluid along with a paradoxically little pronounced pulmonary pathology associated with a rather low chronic retention of nanoparticles in the lungs. There were various manifestations of systemic toxicity, including damage to the liver and kidneys; a likely allergic syndrome as indicated by some cytological signs; transient stimulation of erythropoiesis; and penetration of nickel into the brain from the nasal mucous membrane along the olfactory pathway. Against a picture of mild to moderate chronic toxicity of nickel, its in vivo genotoxic effect assessed by the degree of DNA fragmentation in nucleated blood cells (the RAPD test) was pronounced, tending to increasing with the length of the exposure period. When rats were given orally, in parallel with the toxic exposure, a set of innocuous substances with differing mechanisms of expected bioprotective action, the genotoxic effect of NiO-NPs was found to be substantially attenuated.

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TOXIC EFFECTS ASSESSMENT OF NICKEL OXIDE NANOPARTICLES BY INHALATION

ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT ◽

10.35627/2219-5238/2018-309-12-24-30 ◽

2018 ◽

pp. 24-29 ◽

Cited By ~ 2

Author(s):

B.A. Katsnelson ◽

M.P. Sutunkova ◽

L.I. Privalova ◽

S.N. Solovjeva ◽

V.B. Gurvich ◽

...

Keyword(s):

Nickel Oxide ◽

Redox Balance ◽

Systemic Toxicity ◽

Laboratory Animals ◽

Oxide Nanoparticles ◽

Nickel Oxide Nanoparticles ◽

White Rats ◽

Liver And Kidney ◽

Stimulation Of Erythropoiesis ◽

Nio Nps

The article presents in an experiment obtained principal results based on repeated low-level inhalation exposures of laboratory animals (white rats, outbred) to nickel oxide nanoparticles with a diameter of (23 ± 5) nm, 4 hours a day, 5 times a week for up to 10 months in a «nose only» installation. It was shown that non-specific body reactions to the action of NiO NPs include: diverse manifestations of systemic toxicity with a particularly pronounced influence on liver and kidney function, redox balance, damage to some areas of brain tissue, associated with proven movement of the nanoparticles themselves from the nasal mucosa along the olfactory tract; some cytological signs of probable development for allergic syndrome; paradoxically low severity of pulmonary pathology by pneumoconiotic type explained by a small chronic delay of nanoparticles in the lungs; the genotoxic effect of the organismal level, even at those low levels of chronic exposure, at which systemic toxicity is rather poorly. Along with that, NiO NPs also induce phase-stimulation of erythropoiesis, which is relatively specific for the toxic nickel effects.

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