The effects of superparamagnetic iron oxide nanoparticle exposure on gene expression patterns in the neural stem cells under magnetic field

Background: Due to the excellent reliable traceability and superparamagnetic properties, superparamagnetic iron oxide nanoparticles (SPIOs) are widely used for the applications in the field of biomedicine, including tissue engineering and regenerative medicine. However, the regulation of SPIOs on the gene expressions in the stem cells is not clear. Methods: In this study, by RNA-Seq analysis, we analyzed the gene expression pattern in the neural stem cells (NSCs) treated with SPIOs in the presence or absence of static magnetic field (SMF). Results: It was found that SPIOs with SMF regulated more gene expression in NSCs, while most of these genes have been previously reported to play a crucial role in NSCs fate decision. Conclusions: Our findings reveal the ability of SPIOs and SMF in the regulation of gene expression in NSCs, which may provide an experimental basis for its applications.

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Targeted Delivery of Iron Oxide Nanoparticle-Loaded Human Embryonic Stem Cell-Derived Spherical Neural Masses for Treating Intracerebral Hemorrhage

International Journal of Molecular Sciences ◽

10.3390/ijms21103658 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3658

Author(s):

Min Kyoung Kang ◽

Tae Jung Kim ◽

Young-Ju Kim ◽

Lamie Kang ◽

Jonghoon Kim ◽

...

Keyword(s):

Magnetic Field ◽

Stem Cells ◽

Stem Cell ◽

Iron Oxide ◽

External Magnetic Field ◽

Targeted Delivery ◽

Human Embryonic Stem Cell ◽

Embryonic Stem ◽

Iron Oxide Nanoparticle ◽

Oxide Nanoparticle

This study evaluated the potential of iron oxide nanoparticle-loaded human embryonic stem cell (ESC)-derived spherical neural masses (SNMs) to improve the transportation of stem cells to the brain, ameliorate brain damage from intracerebral hemorrhage (ICH), and recover the functional status after ICH under an external magnetic field of a magnet attached to a helmet. At 24 h after induction of ICH, rats were randomly separated into three experimental groups: ICH with injection of phosphate-buffered saline (PBS group), ICH with intravenous injection of magnetosome-like ferrimagnetic iron oxide nanocubes (FION)-labeled SNMs (SNMs* group), and ICH with intravenous injection of FION-labeled SNMs followed by three days of external magnetic field exposure for targeted delivery by a magnet-embedded helmet (SNMs*+Helmet group). On day 3 after ICH induction, an increased Prussian blue-stained area and decreased swelling volume were observed in the SNMs*+Helmet group compared with that of the other groups. A significantly decreased recruitment of macrophages and neutrophils and a downregulation of pro-inflammatory cytokines followed by improved neurological function three days after ICH were observed in the SNMs*+Helmet group. Hemispheric atrophy at six weeks after ICH was significantly decreased in the SNMs*+Helmet group compared with that of the PBS group. In conclusion, we have developed a targeted delivery system using FION tagged to stem cells and a magnet-embedded helmet. The targeted delivery of SNMs might have the potential for developing novel therapeutic strategies for ICH.

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