scholarly journals Steeping Tin Leaves (Ficus carica) Improves Sperm Quality of Male Mice (Mus musculus) Exposed to Lead Acetate

2022 ◽  
Vol 16 (1) ◽  
Keyword(s):  
Mus Musculus ◽  
Lead Acetate ◽  
Sperm Quality ◽  
Ficus Carica ◽  
Male Mice

scholarly journals Effectiveness of Averrhoa bilimbi leaf extract on spermatogenic cells of mice (Mus Musculus L.) hyperglycemia

2021 ◽  
Vol 4 (2) ◽  
pp. 273-279
Author(s):  
Ida Ayu Manik Damayanti ◽  
Putu Indrayoni ◽  
Ni Wayan Sukma Antari ◽  
Anak Agung Istri Mas Padmiswari
Keyword(s):  
Leaf Extract ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Control Group ◽  
P Value ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Control Group Design ◽  
Post Test

The purpose of this study was to determine the effect of giving Averrhoa bilimbi leaf extract on sperm quality of diabetic mice. This research is a pure experimental (true experimental) with a post-test-only control group design approach. This research was conducted by giving Averrhoa bilimbi leaf extract as a treatment for 42 days in male mice. Sperm quality parameters observed included viability, abnormalities, motility in sperm. In all variables, the results of the data showing a normal distribution with a p-value > 0.05 were then carried out with a parametric test using one-way ANOVA. Averrhoa bilimbi leaf extract can increase the number of spermatogenic cells in male mice with hyperglycemia.

scholarly journals PENGARUH PAPARAN ASAP ROKOK KRETEK TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Immanuel Van Donn Batubara ◽  
Benny Wantouw ◽  
Lydia Tendean
Keyword(s):  
Cigarette Smoke ◽  
Mus Musculus ◽  
Sperm Quality ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Not Given ◽  
Significant Difference ◽  
Key Word ◽  
Randomized Experimental Design

Abstrak: Asap rokok mengandung tiga komponen toksik utama, yaitu karbonmonoksida, nikotin, dan tar yang dapat menyebabkan gangguan pada spermatogenesis. Tujuan penelitian ini adalah untuk melihat pengaruh paparan asap rokok kretek terhadap kualitas spermatozoa mencit. Penelitian ini adalah penelitian eksperimental dengan rancangan acak lengkap. Subyek penelitian sebanyak 25 ekor mencit jantan (Mus musculus) yang dibagi secara acak menjadi 5 kelompok: kelompok P0 tidak diberikan paparan asap rokok, kelompok P1 diberikan paparan asap rokok kretek 1 batang, kelompok P2 diberikan paparan asap rokok kretek 2 batang, kelompok P3 diberikan paparan asap rokok kretek 3 batang, kelompok P4 diberikan paparan asap rokok kretek 4 batang. Perlakuan diberikan selama 30 hari. Hasil penelitian menunjukkan adanya perbedaan yang signifikan pada konsentrasi dan motilitas spermatozoa kelompok yang mendapatkan paparan asap rokok kretek (p<0,05) dibandingkan dengan kelompok kontrol. Morfologi spermatozoa kelompok P2,P3,P4 didapati hasil yang berbeda signifikan dengan kelompok kontrol (p<0,05) tetapi kelompok P1 hasilnya berbeda tak signifikan dengan kelompok kontrol. Paparan asap rokok kretek memberikan pengaruh negatif terhadap kualitas spermatozoa yang disebabkan oleh adanya radikal bebas yang dihasilkan oleh asap rokok. Kata Kunci: asap rokok, kualitas spermatozoa.     Abstract: Cigarette smoke contains three main toxic components, namely carbon monoxide, nicotine, and tar that can cause disturbances in spermatogenesis. The objective of this study was to investigate and observe the effect of cigarette smoke exposure on the quality of spermatozoa of mice. The study was a completely randomized experimental design. The research subject as many as 25 male mice who were randomly divided into 5 groups: group P0 not given exposure, the P1 is given exposure to smoke from 1 bar of cigarette, the P2 is given exposure to smoke from 2 bars of cigarette, the P3 is given exposure to smoke from 3 bars of cigarette and finally the P4 is given exposure to smoke from 4 bars of cigarette. The treatment is given for 30 days. At the end of experiment, results showed a significant difference in the concentration and motility of spermatozoa between the groups exposed to cigarette smoke (p<0,05) compared with the control group. The morphology of spermatozoa group P2,P3,P4, was found substantially different with the control group (p<0,05) but group P1 did not have significant difference in results with the control group. The exposure to cigarette smoke negatively affects sperm quality caused by the free radicals generated by the smoke. Key Word: cigarette smoke, sperm quality.

scholarly journals Pengaruh Pemberian Ekstrak Kijing (Pilsbryoconcha exilis) Pada Pemulihan Kualitas Sperma Mencit (Mus musculus)

2019 ◽  
Vol 3 (1) ◽  
pp. 40-44
Author(s):  
Erna Yunita Sari ◽  
Bhakti Karyadi ◽  
Aceng Ruyani ◽  
Choirul Muslim
Keyword(s):  
Data Analysis ◽  
Mus Musculus ◽  
Sperm Quality ◽  
Statistical Test ◽  
Design Model ◽  
Real Difference ◽  
Randomized Design ◽  
Significant Difference ◽  
Qualitative Descriptive

ABSTRACT[Effect of Kijing Extract (Pilsbryoconcha exilis) on the Recovery of Sperm Quality in Mice (Mus musculus)]. This study aims to determine the effect of Pilsbryoconcha exilis extract on the quality of M. muscullus sperm. The approach used for the study was experiment with a complete randomized design model. In this study, 4 treatments and 5 replications were conducted, including treatment 1 which only administered aquades as control (P0), treatment 2 which only given paracetamol dose 250 mg / kg (P1), and treatment 3 given paracetamol dose 250 mg / kg and P. exilis extract dose 0,46 mg / g (P2) and treatment 4 given paracetamol 250 mg / kg and extract P. exilis 0,69 mg / g (P3). Technique of data analysis using qualitative descriptive and statistical test of One Way Annova parametry, and if obtained significant difference then continued statistic test of Smallest Real Difference. From the results of the study, it was found that P. exilis and paracetamol extract had no effect on sperm quality. Keywords: Sperm; M. muscullus; extract P. exilis; paracetamol.(Received August 14 , 2018; Accepted January 20, 2019; Published February 26, 2019) ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak Pilsbryoconcha exilis terhadap kualitas sperma M. muscullus yang diberi parasetamol. Pendekatan yang digunakan untuk penelitian adalah eksperimen dengan model rancangan acak lengkap (RAL). Pada penelitian ini menggunakan 4 perlakuan dan 5 ulangan meliputi perlakuan 1 yang hanya diberikan aquades merupakan kontrol (P0), perlakuan 2 yang hanya diberikan parasetamol dosis 250 mg/kg bb (P1), dan perlakuan 3 yang diberikan parasetamol dosis 250 mg/kg bb dan ekstrak P. exilis dosisi 0,46 mg/g bb (P2) dan perlakuan 4 yang diberikan parasetamol 250 mg/kg bb dan ekstrak P. exilis 0,69 mg/g bb (P3). Teknik analisa data menggunakan deskriptif kualitatif dan uji statistik parametri One Way Annova, dan jika didapatkan perbedaan yang bermakna maka dilanjutkan uji statistic Beda Nyata Terkecil (BNT). Dari hasil penelitian diketahui pemberian ekstrak P. exilis dan parasetamol berepengaruh tapi tidak signifikan terhadap kualitas sperma. Kata Kunci: Sperma; M. muscullus; ekstrak P. exilis; parasetamol.

scholarly journals PERBEDAAN KUALITAS SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS L) YANG DIBERIKAN VITAMIN C SETELAH PEMAPARAN ASAP ROKOK

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Vergina Claudia ◽  
Edwin De Queljoe ◽  
Lydia Tendean
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Cigarette Smoke ◽  
Mus Musculus ◽  
Sperm Concentration ◽  
Control Group ◽  
Male Mice ◽  
Normal Morphology ◽  
The Mean

Abstract: Vitamin C is an antioxidant that suppresses the oxidative stress caused by cigarette smoke purpose of this study demonstrate whether vitamin C can provide a different quality of sperm male mice Mus musculus L are given exposure to cigarette smoke. Methodology: The study subjects are 32 male mice Mus musculus L, which were randomly divided into 2 groups. The first group was the control group (K) who received treatment exposure from cigarette smoke without giving vitamin C and the second group is the treatment group (P) are given exposure from cigarette smoke and vitamin C dose of 0.40 mg / gBB /day. The treatment during 30 days, on day 31 the mice were terminated, and then examined the quality of spermatozoa motility of spermatozoa, sperm concentration and morphology of spermatozoa. Results: Test oneway ANOVA on the mean motility of spermatozoa showed Ma control group (47,38%), Mb (11,88%), Mc (18,13%), Md (22,63%) and the Ma treatment group (11.68%), Mb (17,04%), Mc (35,77%), Md (16,5%). The mean concentration of spermatozoa obtained 63,34x105/ml control group and the treatment group and the mean morphology of spermatozoa 81,75x105/ml control group showed normal morphology 50% and abnormal morphology 56,189% while the treatment group showed normal morphology 60% and 40% abnormal. Result from Oneway ANOVA test there are differences significant in the morphology of spermatozoa, concentration of spermatozoa, sperm concentration, and sperm morphology between groups (p <0,05). Conclusion: Vitamin C can improve the quality of spermatozoa after exposure to cigarette smoke. Keywords: ciggaratte, vitamin C, quality of spermatozoa.   Abstrak: Vitamin C merupakan  antioksidan yang menekan proses stres oksidatif akibat asap rokok Tujuan penelitian membuktikan apakah vitamin C dapat memberikan perbedaan kualitas spermatozoa mencit jantan Mus Musculus L yang diberi paparan asap rokok. Metodologi: Subyek penelitian 32 ekor mencit jantan Mus Musculus L, yang dibagi secara acak menjadi 2 kelompok. Kelompok pertama adalah kelompok kontrol (K) yang mendapat perlakuan paparan asap rokok tanpa pemberian vitamin C dan kelompok kedua yaitu kelompok perlakuan (P) yang diberi paparan asap rokok dan vitamin C dosis 0,40mg/gBB/hari. Perlakuan berlangsung selama 30 hari, pada hari ke-31 mencit tersebut diterminasi, kemudian dilakukan pemeriksaan kualitas spermatozoa yaitu motilitas spermatozoa, konsentrasi spermatozoa dan morfologi spermatozoa. Hasil: Uji oneway ANOVA pada rerata motilitas spermatozoa kelompok kontrol menunjukan Ma(47.38%), Mb(11.88%), Mc(18.13%), Md(22.63%) dan kelompok perlakuan Ma(11.68%), Mb(17.04%), Mc(35.77%), Md(16.5%). Rerata konsentrasi spermatozoa kelompok kontrol didapatkan 63.34x105/ml, dan kelompok perlakuan 81.75x105/ml dan rerata morfologi spermatozoa kelompok kontrol menunjukkan morfologi normal 50% dan morfologi abnormal 56,189% sedangkan kelompok perlakuan menunjukkan morfologi normal 60% dan abnormal 40%. Hasil Uji oneway ANOVA terdapat perbedaan yang signifikan pada konsentrasi spermatozoa yaitu morfologi spermatozoa, konsentrasi spermatozoa, dan morfologi spermatozoa antar kelompok (p<0.05). Kesimpulan: Pemberian vitamin C dapat memperbaiki kualitas spermatozoa setelah pemaparan asap rokok. Kata kunci: Rokok, vitamin C, kualitas spermatozoa.

PENURUNAN LAJU PENUAAN REPRODUKSI MENCIT JANTAN (Mus muculus.Linn) DENGAN PEMBERIAN EKSTRAK JAHE (Zingiber officinale) DALAM PAKAN

2018 ◽  
Vol 5 (1) ◽  
pp. 1-10
Author(s):  
Sutyarso Sutyarso ◽  
Sumayyah Annida ◽  
M. Kanedi ◽  
Hendri Busman ◽  
Nuning Nurcahyani
Keyword(s):  
Mus Musculus ◽  
Reproductive Function ◽  
Zingiber Officinale ◽  
Male Mice ◽  
Ginger Extract ◽  
Normal Morphology ◽  
Aging Period ◽  
Ginger Rhizome ◽  
Rate Of Decline

The ginger rhizome contains antioxidants and is chemoprotective, therefore we suspect it can decrease the aging rate in the reproductive system. The purpose of this study, to determine the effect of ginger extract (Zingiber officinale) against reproductive aging of male mice (Mus musculus). This study, using 36 male mice (Mus musculus) aged 12-14 months, divided into three groups each 12 tails. Group 1 as controls, groups 2 and 3 were given 50 mg and 100 mg ginger extract / kg of pellets. The pellet is given for 70 days ad libitum. Next, observed the number of spermatogenic cells, as well as the number and quality of spermatozoa. The results showed that ginger extract had an effect on spermatogenic and spermatozoa (P <0,01) cells of mice. The results of preleptotene spermatocyte, pakhiten and spermatid spermatocyte counts were higher, as were the number of spermatozoa, the percentage of viability and motility, and the normal morphology of spermatozoa were more in the group given ginger extract than control (P <0.01). It was concluded that ginger rhizome extract given to mice entering the aging period could inhibit the rate of decline in reproductive function.

scholarly journals Pengaruh Pemberian Ekstrak Kijing (Pilsbryoconcha exilis) Pada Pemulihan Kualitas Sperma Mencit (Mus musculus)

2019 ◽  
Vol 3 (1) ◽  
pp. 40-44
Author(s):  
Erna Yunita Sari ◽  
Bhakti Karyadi ◽  
Aceng Ruyani ◽  
Choirul Muslim
Keyword(s):  
Data Analysis ◽  
Mus Musculus ◽  
Sperm Quality ◽  
Statistical Test ◽  
Design Model ◽  
Real Difference ◽  
Randomized Design ◽  
Significant Difference ◽  
Qualitative Descriptive

ABSTRACT[Effect of Kijing Extract (Pilsbryoconcha exilis) on the Recovery of Sperm Quality in Mice (Mus musculus)]. This study aims to determine the effect of Pilsbryoconcha exilis extract on the quality of M. muscullus sperm. The approach used for the study was experiment with a complete randomized design model. In this study, 4 treatments and 5 replications were conducted, including treatment 1 which only administered aquades as control (P0), treatment 2 which only given paracetamol dose 250 mg / kg (P1), and treatment 3 given paracetamol dose 250 mg / kg and P. exilis extract dose 0,46 mg / g (P2) and treatment 4 given paracetamol 250 mg / kg and extract P. exilis 0,69 mg / g (P3). Technique of data analysis using qualitative descriptive and statistical test of One Way Annova parametry, and if obtained significant difference then continued statistic test of Smallest Real Difference. From the results of the study, it was found that P. exilis and paracetamol extract had no effect on sperm quality. Keywords: Sperm; M. muscullus; extract P. exilis; paracetamol.(Received August 14 , 2018; Accepted January 20, 2019; Published February 26, 2019) ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak Pilsbryoconcha exilis terhadap kualitas sperma M. muscullus yang diberi parasetamol. Pendekatan yang digunakan untuk penelitian adalah eksperimen dengan model rancangan acak lengkap (RAL). Pada penelitian ini menggunakan 4 perlakuan dan 5 ulangan meliputi perlakuan 1 yang hanya diberikan aquades merupakan kontrol (P0), perlakuan 2 yang hanya diberikan parasetamol dosis 250 mg/kg bb (P1), dan perlakuan 3 yang diberikan parasetamol dosis 250 mg/kg bb dan ekstrak P. exilis dosisi 0,46 mg/g bb (P2) dan perlakuan 4 yang diberikan parasetamol 250 mg/kg bb dan ekstrak P. exilis 0,69 mg/g bb (P3). Teknik analisa data menggunakan deskriptif kualitatif dan uji statistik parametri One Way Annova, dan jika didapatkan perbedaan yang bermakna maka dilanjutkan uji statistic Beda Nyata Terkecil (BNT). Dari hasil penelitian diketahui pemberian ekstrak P. exilis dan parasetamol berepengaruh tapi tidak signifikan terhadap kualitas sperma. Kata Kunci: Sperma; M. muscullus; ekstrak P. exilis; parasetamol.

scholarly journals Effects of Chitosan on Sperm Quality of Lead Acetate-Induced Rats

2020 ◽  
Vol 1567 ◽  
pp. 032061
Author(s):  
A Marianti ◽  
W Isnaeni ◽  
N Setiati ◽  
S Sumadi
Keyword(s):  
Lead Acetate ◽  
Sperm Quality

scholarly journals Efek Protektif Zink Terhadap Stres Oksidatif Testis dan Kualitas Sperma Pada Mencit Jantan (Mus Musculus) Setelah Diinduksi Cyclophosphamide

2020 ◽  
Vol 22 (2) ◽  
pp. 63
Author(s):  
Retno Yulianti
Keyword(s):  
Mus Musculus ◽  
Sperm Quality ◽  
Alkylating Agents ◽  
The Body ◽  
Not Given ◽  
Male Mice ◽  
Anti Cancer ◽  
The Mean ◽  
Negative Controls ◽  
Positive Controls

AbstractCyclophosphamide is a class of alkylating agents used for cancer treatment. The side effect of CP is through the depletion mechanism of the GSH cellular pathway which generates free radicals by activation by copper/iron in the body to damage the spermatogenesis process. Zinc has antioxidant enzymes and can bind metallothionein to protect the body from the effects of anti-cancer drugs. The purpose of this study was to determine the protective effect of Zn against testicular oxidative stress and sperm quality in male mice (Mus musculus) after cyclophosphamide induction. This study used 30 male mice divided into six groups, namely negative controls that were not given CP and Zn (K1), positive controls that were given only Zn (K2) and only CP (K3) and groups that were given CP injections (200 mg / Kg) with 3 doses of Zn, namely 25 mg (K4), 50 mg (K5) and 100 mg (K6). The analysis showed that the mean sperm quality in all groups was significantly different (p=0.011). The mean levels of MDA testis in all groups did not differ significantly. There was no relationship between testicular MDA levels with  sperms quality (p=0.800) and the  degree of correlation in a negative direction (R= -0.048). This study concluded that testicular MDA levels are not associated with sperms quality, especially the number of sperms cells. Keywords : cyclophosphamide, malondialdehid, sperms, zinc AbstrakCyclophosphamide  (CP) merupakan  golongan  alkylating  agent  yang  digunakan  untuk pengobatan kanker. Efek samping CP melalui mekanisme deplesi jalur seluler GSH yang menghasilkan radikal bebas dengan aktivasi oleh tembaga atau besi dalam tubuh sehingga merusak proses spermatogenesis. Zink (Zn) memiliki enzim–enzim antioksidan dan mampu mengikat metallothionein untuk melindungi tubuh dari efek obat anti kanker. Tujuan penelitian ini untuk mengetahui efek proteksi zink terhadap stress oksidatif testis dan kualitas sperma pada mencit jantan (Mus musculus) setelah diinduksi cyclophospamid . Penelitian ini menggunakan 30 mencit jantan yang dibagi enam kelompok yaitu kontrol negatif yang tidak diberi CP dan Zn (K1), kontrol positif yang hanya diberi Zn (K2) dan hanya CP (K3) serta kelompok yang diberikan penyuntikan CP (200 mg/Kg) dengan 3 dosis Zn yaitu 25 mg (K4), 50 mg (K5) dan 100 mg (K6). Hasil analisis menunjukkan rerata kualitas sperma (jumlah sel sperma) pada semua kelompok berbeda siginifikan (p=0.011). Rerata kadar MDA testis pada semua kelompok tidak berbeda signifikan. Tidak terdapat hubungan antara kadar MDA testis dengan kualitas sperma (jumlah sel sperma) (p =0.800, p>0.05) dan derajat korelasi ke arah negatif (nilai R= -0.048) Kesimpulan penelitian ini bahwa kadar MDA testis tidak berhubungan dengan kualitas sperma terutama jumlah sel sperma. Kata Kunci: cyclophosphamide, malondialdehid, sperma, zink 

scholarly journals Pengaruh Ekstrak Etanol dan Spinasterol Daun Senggugu (Clerodendron Serratum L.) terhadap Kualitas Sperma Mencit (Mus Musculus L.)

2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Desak Made Malini
Keyword(s):  
Body Weight ◽  
Mus Musculus ◽  
Sperm Quality ◽  
Ethanol Extract ◽  
Dmso Solution ◽  
Epididymal Sperm ◽  
The Public ◽  
Sperm Suspension ◽  
The Family

AbstrakTumbuhan senggugu (C. serratum L.) termasuk famili verbenaceae, merupakan salah satu tumbuhan yang cukup dikenal dimasyarakat sebagai tanaman obat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun senggugu (C. serratum L.) terhadap kualitas sperma mencit (Mus musculus). Penelitian ini merupakan penelitian eksperimental yang menggunakan rancangan percobaan dengan pola Rancangan Acak Lengkap (RAL). Penelitian ini terdiri atas dua kelompok kontrol dan lima kelompok perlakuan dengan lama perlakuan 9 dan 18 hari. Setiap kelompok menggunakan 6 ekor mencit, sehingga jumlah mencit keseluruhan adalah 42 ekor. Parameter yang diamati adalah jumlah, motilitas, abnormalitas dan viabilitas sperma mencit. Data yang diperoleh dianalisis dengan Anava dan dilanjutkan dengan uji Duncan. Hasil penelitian ini menunjukkan bahwa dilihat dari hasil daun senggugu (C. serratum L.) dapat menurunkan kualitas mencit.Kata kunci : senggugu, mencit dan kulaitas sperma.AbstractPlant senggugu (C.serratum L.) belongs to the family Verbenaceae, which has been recogniced by the public as a medicinal plant. One of them is that it can be used as a drug antifertility in men. The purpose of this study is to find out the effect of ethanol extract and spinasterol of Senggugu ©.Serratum L.) leaves to the sperm quality of mouse (M. musculus) The study is conducted using complete random with 7X2 factor repeated 6 times. The treatment given to the ethanol extract is 250 mg/kg body weight, 500 mg/kg body weight and 1000 mg/kg body weight, as with spinasterol the amount is 26 mg/kg body weight and 52 mg/kg body weight, and as its controlling agent distilled water and DMSO solution is used. The treatment is given orally once a day within 9and 18 days as much as 0,5 ml. Cauda epididymal sperm suspension was used to measure the quality of the sperm of mice. Data were analyzed by ANOVA followed by Duncan test. Based on observation and statistical analysis it has been found that theethanol and spinasterol extract treatment of senggugu leaves using a diffrent dosage has shown a significant effects to the parameters being studied namely increased abnormality of sperm and decreased in number of sperm, percentage of motolityand viability sperm.Keyword : Clerodendron serattum, senggugu, Mus musculus, Spermatozoa, Ethanol extract, Spinasterol

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