scholarly journals Hidróxidos dobles estratificados: aplicación en la inhibición de coliformes

2021 ◽  
Vol 4 (3) ◽  
pp. 157-170
Author(s):  
Roberto Guerra-González ◽  
Martha Angélica Lemus-Solorio ◽  
José Luis Rivera-Rojas ◽  
Alfonso Lemus-Solorio ◽  
América Abisay Mondragón-Herrera ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhi ◽  
E Coli

En este trabajo se estudió la preparación de diferentes materiales híbridos orgánicos / inorgánicos y su evaluación como bactericidas frente a Escherichia coli (E. coli) y Salmonella typhi (S. typhi). El objetivo principal de la presente investigación fue sintetizar y caracterizar materiales híbridos biocompatibles que inmovilizan moléculas con actividad antibacteriana en matrices lamelares inorgánicas basadas en dobles hidróxidos lamelares inorgánicos y evaluar su actividad antibacteriana frente a Escherichia coli (E. coli) y Salmonella typhi (S. typhi). Los materiales híbridos consisten en la asociación de un doble hidróxido laminar inorgánico, o compuestos tipo hidrotalcita, con moléculas orgánicas con actividad antibacteriana, alojadas en sólidos. Los hidróxidos dobles lamelares (LDH) son estructuras sintéticas formadas por películas de hidróxido metálico con carga positiva que se estabilizan con aniones interlaminares. Se han estudiado diferentes materiales híbridos a partir de compuestos tipo hidrotalcita, como MgAl, ZnAl y MgFeAl, que contienen especies orgánicas de cefalexina sódica y ácidos nalidíxico y pipemídico. La intercalación de los diferentes aniones se realizó mediante uno de los diferentes métodos existentes: la coprecipitación de los compuestos tipo hidrotalcita en presencia de la molécula de interés y por el efecto memoria. La caracterización de los materiales se realizó mediante difracción de rayos X, espectroscopia de IR y resonancia magnética nuclear sólida, analizando específicamente los núcleos 27Al y 13C, y análisis termogravimétrico. La evaluación de la actividad antibacteriana de estos materiales se evaluó en cultivos de cepas de Escherichia coli (E. coli) y Salmonella typhi (S. typhi). La actividad antibacteriana de los sistemas híbridos probados no siempre es una función directa de la cantidad de antibiótico intercalado. Se obtuvo que el LDH ZnAl-NADmem presenta una liberación controlada, ya que cuando el material fue expuesto tres veces contra la bacteria Escherichia coli (E. coli), continuó eliminando bacterias, presentando un efecto bacteriostático en la tercera exposición, ya que no eliminar las bacterias.

scholarly journals Níveis de sensibilidade de enterobactérias, em particular Salmonella typhi, a rifamicina S.V.

1969 ◽  
Vol 3 (2) ◽  
pp. 87-93
Author(s):  
Ivone R. Suassuna ◽  
I. Suassuna ◽  
C. E. de V. Serpa
Keyword(s):  
Escherichia Coli ◽  
Proteus Mirabilis ◽  
Salmonella Typhi ◽  
E Coli

Em face à predominante eliminação biliar da rifamicina S.V. atingindo concentrações muitas vêzes superiores aos níveis séricos obtidos com as doses terapêuticas, e pelo possível interêsse dessa verificação para o tratamento dos portadores biliares crônicos de Salmonella typhi determinou-se a concentração mínima inibitória de 165 estirpes de enterobactérias, incluindo 77 amostras de S. typhi. Foi verificado que a maioria das cepas de Escherichia coli, Shigella e Proteus mirabilis correspondiam a uma concentração inibitória mínima entre 33 a 65 μg/ml. Entre 65 e 128 μg/ml foram determinadas as concetrações inibitórias mínimas da maioria das outras espécies de Proteus, de Providencia e de Klebsiella. Para Salmonella e Enterobacter o limite mínino de sensibilidade foi, em regra, igual ou superior a 128 μg/ml. Diferenças mais acentuadas de comportamento entre as enterobactérias foram observadas quanto à ação bactericida da rifamicidas S.V. De uma maneira geral, para E. coli e Shigella, as concentrações inibitórias mínimas já referidas. Para as espécies de Proteus e Providencia houve variação maior de comportamento, mas tendência a que o efeito bactericidas fôsse encontrado em concentrações que correspondiam a 4 vêzes as bacteriostáticas para as mesmas espécies. Finalmente, de modo pouco feliz para os propósitos visados, em Salmonella, com a inclusão de S. typhi, não foi atingido um efeito bactericida, com as mais altas concentrações usadas as quais corresponderam em média a 6 vêzes as concentrações bacteriostáticas para esse gênero.

scholarly journals UJI EFEKTIFITAS ANTIBAKTERI EKSTRAK KULIT BATANG JAMBU MONYET (Bellucia pentamera Naudin) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Salmonella typhi

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Farid Priandi ◽  
Fathul Yusro ◽  
Farah Diba ◽  
Yeni Mariani ◽  
. Nurhaida
Keyword(s):  
Escherichia Coli ◽  
Typhoid Fever ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Negative Effects ◽  
E Coli ◽  
Extract Concentration ◽  
The People ◽  
The Media

People still use plants as a medicine to overcome a disease, because it is easily to obtain and relatively does not provide significant negative effects. One of the plants utilized by the people is Bellucia pentamera Naudin (B. pentamera). Diarrhea and typhoid fever are diseases that often assault Indonesian people. The bacterias were used in this study were Escherichia coli (E. coli) and Salmonella typhi (S. typhi). This study aimed to analyze the antibacterial effectiveness of B. pentamera bark extracts against E. coli and S. typhi, by Kirby-Bauer diffusion method with PCA as the media. The highest extract concentration against E. coli is 400 mg/ml possess an inhibition of 20±1 mm, and towards S. typhi is 80 mg/ml which possess an inhibition of 24.67±0,58 mm.Keywords: Bellucia pentamera, Disc Diffussion, Efectiveness, Escherichia coli, Salmonella typhi.

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals Synthesis, Characterization and Antimicrobial Activities of Co (III) and Fe (III) Complexes of Imino-N-heterocyclic Carbenes Ligands

2021 ◽  
pp. 59-67
Author(s):  
Samaila Abubakar ◽  
Musa Muktari ◽  
Rejoice Atiko
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Heterocyclic Carbenes ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria

The synthesis and antimicrobial application of Co (III) and Fe (III) complexes of imine functionalized N-heterocyclic carbene (Imino-NHC) ligands is reported. The ligand precursors 1-(2-[(hydroxyl-benzylidene)-amino]-ethyl)-3-R-3H-imidazol-1-ium bromide where R = pyridyl (1a) and benzyl (1b) have been reported in our previous work. The in-situ generated ligands of 1a and 1b have been successfully coordinated to CoBr2 and [FeI(Cp)(CO)2] leading to the isolation of air-stable N^C^N^O four coordinate Co(III)  complex 2 and a six-coordinate Fe(III) complex 3. The synthesised complexes were both found to be NMR inactive hence were characterize using FTIR and LRMS. The complexes were screened for antimicrobial activities against four gram-negative bacteria Escherichia Coli (E-coli), Shigella, Klebsiella pneumoniae (K. Pneumoniae) and Salmonella typhi (S. typhi) and a gram positive bacteria Staphylocossus aureus (S. aureus). The antimicrobial test was conducted using disc diffusion methods and based on the concentrations of 100, 200, 300, 400 and 500 µg/ mL, significant activities were recorded for both cobalt and the iron complexes.

Effect of Selected Plant Essential Oils or Their Constituents and Modified Atmosphere Packaging on the Radiosensitivity of Escherichia coli O157:H7 and Salmonella Typhi in Ground Beef

2008 ◽  
Vol 71 (3) ◽  
pp. 516-521 ◽  
Author(s):  
M. TURGIS ◽  
J. BORSA ◽  
M. MILLETTE ◽  
S. SALMIERI ◽  
M. LACROIX
Keyword(s):  
Escherichia Coli ◽  
Essential Oils ◽  
Modified Atmosphere Packaging ◽  
Ground Beef ◽  
Radiation Sensitivity ◽  
Salmonella Typhi ◽  
Modified Atmosphere ◽  
Plant Essential Oils ◽  
E Coli

Twenty-six different essential oils were tested for their efficiency to increase the relative radiosensitivity of Escherichia coli and Salmonella Typhi in medium-fat ground beef (23% fat). Ground beef was inoculated with E. coli O157:H7 or Salmonella (106 CFU/g), and each essential oil or one of their main constituents was added separately at a concentration of 0.5% (wt/wt). Meat samples (10 g) were packed under air or under modified atmosphere and irradiated at doses from 0 to 1 kGy for the determination of the D10-value of E. coli O157:H7, and from 0 to 1.75 kGy for the determination of the D10-value of Salmonella Typhi. Depending on the compound tested, the relative radiation sensitivity increased from 1 to 3.57 for E. coli O157:H7 and from 1 to 3.26 for Salmonella Typhi. Addition of essential oils or their constituents before irradiation also reduced the irradiation dose needed to eliminate both pathogens. In the presence of Chinese cinnamon or Spanish oregano essential oils, the minimum doses required to eliminate the bacteria were reduced from 1.2 to 0.35 and from 1.4 to 0.5 for E. coli O157:H7 and Salmonella Typhi, respectively. Cinnamon, oregano, and mustard essential oils were the most effective radiosensitizers.

scholarly journals Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

2021 ◽  
Vol 21 (1) ◽  
pp. 281
Author(s):  
Muhammad Zaidan Tsani Ariandi ◽  
Meiskha Bahar ◽  
Hany Yusmaini ◽  
Fajriati Zulfa ◽  
Cut Fauziah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
P Value ◽  
E Coli ◽  
Botanical Gardens

Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.  

scholarly journals SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BATANG (Eusideroxylon zwageri) TERHADAP Escherichia coli DAN Salmonella typhi

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hana Wila ◽  
Fathul Yusro ◽  
Yeni Mariani
Keyword(s):  
Escherichia Coli ◽  
Moisture Content ◽  
Typhoid Fever ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Phytochemical Analysis ◽  
Strong Inhibition ◽  
E Coli ◽  
Growth Of Bacteria ◽  
Eusideroxylon Zwageri

Diarrhea and typhoid fever are diseases commonly suffered by people in Indonesia, and bacteria such as Escherichia coli (diarrhea) and Salmonella typhi (typhoid fever) are one of those diseases causative agents. Various plants are potentially used as anti-bacterial including ulin (Eusideroxylon zwageri). The aims of this study were to analysis the yield and potential secondary metabolite content in ethanol extract of E. zwageri, and determine the optimal concentration in inhibits the growth of bacteria E. coli and S. typhi. The methods used in this study were measure the moisture content of E. zwageri wood bark powder, extraction with ethanol solvent, measure the yield percentage, qualitative of phytochemical analysis, and antibacterial assays. The results showed that the yield of ethanol extract of E. zwageri is 21,63% with 7,6% of moisture content. The extract positively contained alkaloids, flavonoids, tannins, saponins, phenolics and terpenoids. The optimum concentration in inhibits the growth of bacteria E. coli is 200 mg/ml and categorized as very strong inhibition response, and 15 mg/ml is concentration used in inhibits the S. typhi and categorized as strong inhibition response.Keywords: Antibacterial activity, Escherichia coli, Eusideroxylon zwageri, phytochemical screening, Salmonella typhi.

scholarly journals In-Vitro Evaluation of Antimicrobial Activities of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans Nosodes

Homeopathy ◽  
2021 ◽  
Author(s):  
Renuka Munshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
E Coli ◽  
Inhibition Of Growth

Abstract Background This study presents the results of the minimum inhibitory concentration (MIC) assay of a series of nosodes: namely Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans. Each was tested against its corresponding infection as well as cross infections. Methods In-vitro efficacy of polyvalent nosodes was tested using the MIC assay technique. The nosodes, namely C. albicans polyvalent nosode (35c, 100c), N. gonorrhoeae (35c), K. pneumoniae (35c, 100c), E. coli polyvalent nosode (35c, 100c) and Salmonella typhi polyvalent nosode (30c, 100c), were tested along with positive and negative controls. Nosodes were studied in different potencies and at 1:1 dilution. Results C. albicans polyvalent nosode 35c, 100c, N. gonorrhoeae 35c, and positive control amphotericin B showed inhibition of the growth of C. albicans species. K. pneumoniae 35c, E. coli polyvalent nosode 100c, and meropenem (positive control) showed inhibition of the growth of K. pneumoniae; this effect was not seen with ceftriaxone, ofloxacin and amoxicillin antibiotics. E. coli polyvalent nosode 30c in 10% alcohol (direct and dilution 1:1) and the positive controls ciprofloxacin, ofloxacin, and amoxicillin showed inhibition of the growth of E. coli. The S. typhi polyvalent nosode 30c in 10% alcohol showed inhibition of growth of S. typhi. Conclusion This study reveals that the tested nosodes exhibited antibacterial potential against the corresponding micro-organisms and against other selected organisms studied using this assay.

scholarly journals Evaluación antibacteriana y perfil cromatográfico (CG-EM) de fracciones de hojas y tallo de Calotropis procera (Linn).

2018 ◽  
Vol 2 (2) ◽  
pp. 19-25
Author(s):  
Asoso Oluwakemi-Sola ◽  
Oladunmoye Muftau-Kolawole ◽  
Ogundare Ayodele Oluyemisi
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Calotropis Procera ◽  
E Coli

La medicina tradicional, también conocida como medicina ancestral o popular, comprende sistemas de conocimientos que se desarrollaron a lo largo de generaciones dentro de varias sociedades antes de la era de la medicina moderna. La cromatografía en columna fue utilizada para recolectar fracciones  crudas; se utilizaron disolventes como éter de petróleo, cloroformo y metanol. Las actividades antibacterianas de las fracciones crudas de Calotropis procera (hojas y tallo) se evaluaron en este estudio, utilizando algunos microorganismos seleccionados como Escherichia coli, Shigella dysenteriae ATCC 24162, Salmonella typhi y Klebsiella pneumoniae ATCC 34089; para tal fin, la planta fue extraída usando  acetona y metanol acuoso. El método de difusión en disco de papel fue usado después de que la zona de inhibición alrededor de los discos fue estimada. Los resultados mostraron que en las hojas de la planta, E. coli con la fracción acuoso-metanol y K. pneumoniae ATCC 34089 con la fracción del éter de petróleo-acetona, tuvieron el rendimiento más alto de 30mm respectivamente;  mientras que en el  tallo, K. pneumoniae ATCC 34089 con la fracción del metanol-metanol tuvo el rendimiento más alto de 25mm. La elucidación estructural de los compuestos bioactivos en los extractos fue evaluada usando CG-EM, identificándose compuestos químicos como fenol, palmitato metílico, ácido ftálico, ácido 9-octadecenoico y otros compuestos conocidos como antimicrobianos valiosos, con actividades biológicas y propiedades antioxidantes. Las hojas y el tallo han presentado buenos compuestos químicos, que pueden ser responsables del efecto antimicrobiano observado.

Radiosensitization of Escherichia coli and Salmonella Typhi in Ground Beef

2004 ◽  
Vol 67 (6) ◽  
pp. 1157-1162 ◽  
Author(s):  
F. CHIASSON ◽  
J. BORSA ◽  
B. OUATTARA ◽  
M. LACROIX
Keyword(s):  
Escherichia Coli ◽  
Ascorbic Acid ◽  
Pathogenic Bacteria ◽  
Ground Beef ◽  
Relative Sensitivity ◽  
Salmonella Typhi ◽  
Modified Atmosphere ◽  
Tetrasodium Pyrophosphate ◽  
E Coli

The radiosensitization of two pathogenic bacteria, Escherichia coli and Salmonella Typhi, was evaluated in the presence of thyme and its principal essential oil constituents (carvacrol and thymol) in ground beef. Ground beef was inoculated with E. coli or Salmonella Typhi (105 CFU/g), and each compound was added separately at various concentrations (0 to 3.5%, wt/wt). The antimicrobial potential of carvacrol, thymol, and thyme was evaluated in unirradiated meat by determining the MIC in percentage (wt/wt) after 24 h of storage at 4 ± 1°C. Results showed a MIC of 0.88 ± 0.12%, 1.14 ± 0.05%, and 2.33 ± 0.32% for E. coli in the presence of carvacrol, thymol, and thyme, respectively. MICs of 1.15 ± 0.02%, 1.60 ± 0.01%, and 2.75 ± 0.17% were observed for Salmonella Typhi in the presence of the same compounds, respectively. The best antimicrobial compound (i.e., carvacrol) was selected and added to the sterilized ground beef along with ascorbic acid (0.5%, wt/wt) and tetrasodium pyrophosphate (0.1%, wt/wt). Meat samples (10 g) were packed in air and then irradiated in a 60Co irradiator at doses of 0 to 0.7 kGy for the determination of E. coli radiation D10 and 0 to 2.25 kGy for the determination of Salmonella Typhi radiation D10. Addition of carvacrol increased the relative sensitivity of both bacteria 2.2 times. The radiation D10 was reduced from 0.126 ± 0.0039 to 0.057 ± 0.0015 kGy for E. coli and from 0.519 ± 0.0308 to 0.235 ± 0.0158 kGy for Salmonella Typhi. The addition of tetrasodium pyrophosphate did not affect significantly (P > 0.05) the radiosensitization of either bacterium. However, the presence of ascorbic acid in the media reduced significantly (P ≤ 0.05) the radiosensitivity of both bacteria. An additive effect of carvacrol addition and packaging under modified atmosphere conditions (60% O2–30% CO2–10% N2) was also observed on bacterial radiosensitization at 4°C. Compared with the control packed under air, modified atmosphere packaging conditions in the presence of carvacrol and tetrasodium pyrophosphate improved the relative sensitivity of E. coli by 2.7 times and Salmonella Typhi by 9.9 times.

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