Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

10.3791/59850 ◽  
2019 ◽  
Author(s):  
Wilson Pak-Kin Lou ◽  
Liqun Wang ◽  
Cheng Long ◽  
Lei Liu ◽  
Ji-Feng Fei
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Knock Out ◽  
Cas9 Protein ◽  
Direct Gene

Effect of stiffness change of transplanted induced neural stem cells after spinal cord injury using silk hydrogel

2017 ◽  
Vol 381 ◽  
pp. 286
Author(s):  
G. Davaa ◽  
J.Y. Hong ◽  
J. Olmos Buitrago ◽  
H.W. Kim ◽  
J.K. Hyun
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Cord Injury ◽  
Induced Neural Stem Cells ◽  
Silk Hydrogel

scholarly journals Immunosuppressants Affect Human Neural Stem Cells In Vitro but Not in an In Vivo Model of Spinal Cord Injury

2013 ◽  
Vol 2 (10) ◽  
pp. 731-744 ◽  
Author(s):  
Christopher J. Sontag ◽  
Hal X. Nguyen ◽  
Noriko Kamei ◽  
Nobuko Uchida ◽  
Aileen J. Anderson ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
In Vivo Model ◽  
Human Neural Stem Cells ◽  
Cord Injury

Targeted Treatment of Experimental Spinal Cord Glioma With Dual Gene-Engineered Human Neural Stem Cells

Neurosurgery ◽  
2015 ◽  
Vol 79 (3) ◽  
pp. 481-491 ◽  
Author(s):  
Alexander E. Ropper ◽  
Xiang Zeng ◽  
Hariprakash Haragopal ◽  
Jamie E. Anderson ◽  
Zaid Aljuboori ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Growth Rate ◽  
Neural Stem Cells ◽  
Tumor Growth ◽  
Rat Model ◽  
Weight Bearing ◽  
Tumor Growth Rate ◽  
Intramedullary Spinal Cord ◽  
Human Neural Stem Cells

Abstract BACKGROUND There are currently no satisfactory treatments or experimental models showing autonomic dysfunction for intramedullary spinal cord gliomas (ISCG). OBJECTIVE To develop a rat model of ISCG and investigate whether genetically engineered human neural stem cells (F3.hNSCs) could be developed into effective therapies for ISCG. METHODS Immunodeficient/Rowett Nude rats received C6 implantation of G55 human glioblastoma cells (10K/each). F3.hNSCs engineered to express either cytosine deaminase gene only (i.e., F3.CD) or dual genes of CD and thymidine kinase (i.e., F3.CD-TK) converted benign 5-fluorocytosine and ganciclovir into oncolytic 5-fluorouracil and ganciclovir-triphosphate, respectively. ISCG rats received injection of F3.CD-TK, F3.CD, or F3.CD-TK debris near the tumor epicenter 7 days after G55 seeding, followed with 5-FC (500 mg/kg/5 mL) and ganciclovir administrations (25 mg/kg/1 mL/day × 5/each repeat, intraperitoneal injection). Per humane standards for animals, loss of weight-bearing stepping in the hindlimb was used to determine post-tumor survival. Also evaluated were autonomic functions and tumor growth rate in vivo. RESULTS ISCG rats with F3.CD-TK treatment survived significantly longer (37.5 ± 4.78 days) than those receiving F3.CD (21.5 ± 1.75 days) or F3.CD-TK debris (19.3 ± 0.85 days; n = 4/group; P <.05, median rank test), with significantly improved autonomic function and reduced tumor growth rate. F3.DC-TK cells migrated diffusively into ISCG clusters to mediate oncolytic effect. CONCLUSION Dual gene-engineered human neural stem cell regimen markedly prolonged survival in a rat model that emulates somatomotor and autonomic dysfunctions of human cervical ISCG. F3.CD-TK may provide a novel approach to treating clinical ISCG.

Engraftment, migration and differentiation of neural stem cells in the rat spinal cord following contusion injury

Cytotherapy ◽  
2010 ◽  
Vol 12 (3) ◽  
pp. 313-325 ◽  
Author(s):  
Siobhan S. McMahon ◽  
Silke Albermann ◽  
Gemma E. Rooney ◽  
Georgina Shaw ◽  
Yolanda Garcia ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Contusion Injury

scholarly journals Synergistic Effect of Neural Stem Cells and Olfactory Ensheathing Cells on Repair of Adult Rat Spinal Cord Injury

2010 ◽  
Vol 19 (10) ◽  
pp. 1325-1337 ◽  
Author(s):  
Gan Wang ◽  
Qiang Ao ◽  
Kai Gong ◽  
Huancong Zuo ◽  
Yandao Gong ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Synergistic Effect ◽  
Neural Stem Cells ◽  
Olfactory Ensheathing Cells ◽  
Rat Spinal Cord ◽  
Adult Rat ◽  
Cord Injury ◽  
Adult Rat Spinal Cord

(285) Spinal cord-derived neural stem cells restore hyperalgesic responses previously diminished by chemical ablation of NK-1 expressing neurons in the rat spinal cord

2015 ◽  
Vol 16 (4) ◽  
pp. S47
Author(s):  
G. Strichartz ◽  
J. Wang ◽  
S. Khasabov ◽  
K. Kojima ◽  
D. Simone ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Chemical Ablation

A collagen microchannel scaffold carrying paclitaxel-liposomes induces neuronal differentiation of neural stem cells through Wnt/β-catenin signaling for spinal cord injury repair

Biomaterials ◽  
2018 ◽  
Vol 183 ◽  
pp. 114-127 ◽  
Author(s):  
Xiaoran Li ◽  
Caixia Fan ◽  
Zhifeng Xiao ◽  
Yannan Zhao ◽  
Haimin Zhang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Neuronal Differentiation ◽  
Injury Repair ◽  
Cord Injury

Transplantation of collagen sponge-based three-dimensional neural stem cells cultured in the RCCS system facilitate locomotor functional recovery in spinal cord injury animals

2022 ◽  
Author(s):  
Jianwu Dai ◽  
Yunlong Zou ◽  
Yanyun Yin ◽  
Zhifeng Xiao ◽  
Yannan Zhao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Three Dimensional ◽  
Collagen Sponge ◽  
Cellular Functions ◽  
Cord Injury ◽  
Cells Cultured

Numerous studies have indicated that microgravity induces various changes in the cellular functions of neural stem cells (NSCs), and the use of microgravity to culture tissue engineering seed cells for...

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