scholarly journals The effect of biofloc and clear water at low and high salinity concentration on growth performance and antioxidant response of wild juveniles of Atlantic white shrimp Penaeus setiferus

2021 ◽  
Vol 49 (3) ◽  
pp. 451-464
Author(s):  
Manuel Valenzuela-Jiménez ◽  
Diana Aguilera-Rivera ◽  
Wilson Wasielesky Jr. ◽  
Idelette Hernández-López ◽  
Gabriela Rodríguez-Fuentes ◽  
...  
Keyword(s):  
Redox Balance ◽  
High Water ◽  
Antioxidant Response ◽  
Water Salinity ◽  
White Shrimp ◽  
Hepatosomatic Index ◽  
Clear Water ◽  
Glutathione S Transferase ◽  
Biomarker Response ◽  
Penaeus Setiferus

The effect of biofloc (BFT) and clear water (CW) at low (ls) and high-water salinity (hs) in wild juveniles of Penaeus setiferus were evaluated. Four treatments were implemented: hsBFT, lsBFT, hsCW, and lsCW. After 45 days, final weight (FW), weight gain (WG), hepatosomatic index (HSI), and survival rate (SR) were evaluated. Antioxidant activity in muscle and hepatopancreas were measured: superoxide dismutase (SOD), catalase (CAT), glutathione-S transferase (GST), lipid peroxidation (LPO), protein oxidation (PO). The FW and SR was low for lsCW (P < 0.05), whereas the WG and HSI was high in hsBFT and lsBFT (P < 0.05). The integrative biomarker response index (IBR) showed differences between muscle (M) and hepatopancreas (HP). Results showed an increase of SOD and CAT measured from HP for lsBFT treatment. For M, CAT and GST were high only in BFT (regardless of the water salinity). LPO determined from M and HP recorded the high values at hs and ls, respectively. The PO determined in HP showed substantial damage in BFT (regardless of the salinity), whereas the same determination from M was only important for lsCW treatment. When the analyses of variance (ANOVA) were performed, SOD, LPO, and PO were significant in response to salinity (P < 0.05). In contrast, for CAT, the higher activity was due to the rearing system and salinity interaction (P < 0.05). Results suggest that BFT could diminish the stress by stimulating the antioxidant system to maintain the redox balance through a higher enzyme activity and decrease damage in the proteins and lipids of the cells.

scholarly journals Health parameters and antioxidant response in Black Sea whiting Merlangius merlangus euxinus (Nordmann, 1840) parasitized by nematode Hysterothylacium aduncum (Rud., 1802)

2019 ◽  
Vol 9 (4) ◽  
pp. 700-709
Author(s):  
Ekaterina Skuratovskaya ◽  
Ekaterina Skuratovskaya ◽  
Andrey Zav'yalov ◽  
Irina Rudneva
Keyword(s):  
Black Sea ◽  
Defense Mechanisms ◽  
Parasitic Infection ◽  
Antioxidant Response ◽  
Infected Fish ◽  
Hepatosomatic Index ◽  
Antioxidant Enzyme Activities ◽  
Glutathione S Transferase ◽  
Merlangius Merlangus ◽  
Hysterothylacium Aduncum

Biometrical and biochemical parameters in healthy and parasitized by nematode Hysterothylacium aduncum Black Sea whiting Merlangius merlangus euxinus were studied. The increase of hepatosomatic index and decrease of spleen index in infected fish were found, while condition factor was similar in both groups. Antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), peroxidase (PER), glutathione reductase (GR) and glutathione-S-transferase (GST)) were higher in red blood cells in the parasitized fish as compared with the healthy animals. In the liver, the antioxidant response was not uniform: activities of SOD and GST decreased and activities of PER, GR and CAT increased in the infected individuals. The obtained results reflect host defense against oxidative stress caused parasitic infection. The tissue specific fluctuations of antioxidant response in parasitized and healthy fish are discussed. Data in this study can be used for understanding the defense mechanisms of fish against parasites.

scholarly journals Gonadal Antioxidant Responses to Seawater Acidification and Hypoxia in the Marine Mussel Mytilus Coruscus

2021 ◽  
Author(s):  
Guangen Xu ◽  
Hui Kong ◽  
Xueqing Chang ◽  
Hui Chen ◽  
Yuewen Deng ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Negative Impact ◽  
Gonad Development ◽  
Antioxidant Response ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Antioxidant Responses ◽  
Seawater Acidification ◽  
Mytilus Coruscus ◽  
Biomarker Response

Abstract This study investigated the combined effects of seawater acidification and hypoxia on the gonadal antioxidant response of the thick shell mussel Mytilus coruscus mainly distributed along the Shengsi Island, East China Sea, where hypoxia and pH fluctuations frequently occur in summer. Mussels were exposed to three pH levels (8.1, 7.7 and 7.3) and two dissolved oxygen (DO) levels (6 and 2 mg L− 1) for 21 days following a 10-day recovery. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione (GSH), glutathione S-transferase (GST) and malondialdehyde (MDA) in gonad and gonad surface area (GSA) were measured at day 21 and 31. Among all the parameters, there was no significant change in SOD activity. GSA and the activity of CAT and GST were decreased under acidification and hypoxia, but GPX, GSH and MDA were increased. PCA showed that the changes were influenced by pH more than DO. Interaction between acidification and hypoxia was found significant on GPX activity and GSA. Integrated biomarker response (IBR) analysis demonstrated that acidification and hypoxia impaired mussel’s antioxidant system and increased oxidative damage. Our results clearly showed that acidification and hypoxia synergistically exert negative impact on the antioxidant system and gonad development of mussels, and the effect of acidification was more significant.

scholarly journals Integrated Biomarker Response for Environmental Assessment Using the Gastropod Phorcus turbinatus along the Northern and the Northeastern Coasts of Tunisia

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 529
Author(s):  
Wafa Boulajfene ◽  
Montassar Lasram ◽  
Sabiha Zouari-Tlig
Keyword(s):  
Condition Factor ◽  
Monitoring Program ◽  
Biological Response ◽  
Temporal Variations ◽  
Chemical Pollution ◽  
Glutathione S Transferase ◽  
Biological Responses ◽  
Biomarker Response ◽  
Polycyclic Aromatic ◽  
Bio Monitoring

This work aims to assess the spatial and temporal variations of four biomarkers activities and to integrate their biological responses in a battery using the gastropod Phorcus turbinatus. The monitoring was carried out during the period between April 2014 and January 2015 at six stations along the northern and the northeastern coasts of Tunisia. The Fulton condition factor was estimated and the activities of catalase, acetylcholinesterase and glutathione-S-transferase were evaluated by spectrophotometry. A multi-biomarker battery approach was used to assess ecosystems’ condition and to identify environmental impacts on the organisms. The results suggest that the enzymatic activities of CAT and GST depend especially on seasons. As for AChE activity, it was similar between seasons and stations. The values of the integrated biological response were maximal at Jarzouna in summer and at Sidi Daoued in winter, indicating the presence of severe stressors suffered by the organisms. This perturbation may be due to the enrichment of the waters by xenobiotics, namely polycyclic aromatic hydrocarbons, insecticides, phosphate wastes, PCBs and pesticides. Thus, P. turbinatus seems to be a good bioindicator of chemical pollution, constituting an adequate tool for a bio-monitoring program.

scholarly journals Stress Responses in Down Syndrome Neurodegeneration: State of the Art and Therapeutic Molecules

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 266
Author(s):  
Chiara Lanzillotta ◽  
Fabio Di Domenico
Keyword(s):  
Down Syndrome ◽  
Stress Response ◽  
Stress Responses ◽  
Redox Balance ◽  
Antioxidant Response ◽  
Chromosome 21 ◽  
Therapeutic Approaches ◽  
Genomic Disorder ◽  
Therapeutic Molecules ◽  
Early Alzheimer’S Disease

Down syndrome (DS) is the most common genomic disorder characterized by the increased incidence of developing early Alzheimer’s disease (AD). In DS, the triplication of genes on chromosome 21 is intimately associated with the increase of AD pathological hallmarks and with the development of brain redox imbalance and aberrant proteostasis. Increasing evidence has recently shown that oxidative stress (OS), associated with mitochondrial dysfunction and with the failure of antioxidant responses (e.g., SOD1 and Nrf2), is an early signature of DS, promoting protein oxidation and the formation of toxic protein aggregates. In turn, systems involved in the surveillance of protein synthesis/folding/degradation mechanisms, such as the integrated stress response (ISR), the unfolded stress response (UPR), and autophagy, are impaired in DS, thus exacerbating brain damage. A number of pre-clinical and clinical studies have been applied to the context of DS with the aim of rescuing redox balance and proteostasis by boosting the antioxidant response and/or inducing the mechanisms of protein re-folding and clearance, and at final of reducing cognitive decline. So far, such therapeutic approaches demonstrated their efficacy in reverting several aspects of DS phenotype in murine models, however, additional studies aimed to translate these approaches in clinical practice are still needed.

scholarly journals Effects of Ginger Phenylpropanoids and Quercetin on Nrf2-ARE Pathway in Human BJ Fibroblasts and HaCaT Keratinocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Ermin Schadich ◽  
Jan Hlaváč ◽  
Tereza Volná ◽  
Lakshman Varanasi ◽  
Marián Hajdúch ◽  
...  
Keyword(s):  
Constitutive Expression ◽  
Antioxidant Response ◽  
Luciferase Reporter ◽  
Related Factor ◽  
Hacat Cells ◽  
Hacat Keratinocytes ◽  
Glutathione S Transferase ◽  
Detoxification Enzyme ◽  
Downstream Target ◽  
Downstream Effector

Quercetin and phenylpropanoids are well known chemoprotective compounds identified in many plants. This study was aimed at determining their effects on activation of Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant response element (Nrf2-ARE) signalling pathway and expression of its important downstream effector phase II detoxification enzyme glutathione-S-transferase P1 (GSTP1) in BJ foreskin fibroblasts and skin HaCaT keratinocytes. Cell lines and their corresponding Nrf2-ARE luciferase reporter cells were treated by ginger phenylpropanoids and quercetin for 10 h and the level of Nrf2 activity was subsequently determined. Both, ginger phenylpropanoids and quercetin, significantly increased the level of Nrf2 activity. Subsequent western blot analyses of proteins showed the increased expression level of glutathione-S-transferase P1 (GSTP1) in BJ cells but not in HaCaT cells. Such phenomenon of unresponsive downstream target expression in HaCaT cells was consistent with previous studies showing a constitutive expression of their GSTP1. Thus, while both ginger phenylpropanoids and quercetin have the property of increasing the level of Nrf2 both in HaCaT and in BJ cells, their effects on its downstream signalling were mediated only in BJ cells.

Organoleptic, Bacterial, and Chemical Characteristics of Penaeid Shrimp Subjected to Short-Term High-Temperature Holding

1977 ◽  
Vol 40 (4) ◽  
pp. 256-260 ◽  
Author(s):  
BRYANT F. COBB ◽  
CHIAPING S. YEH ◽  
FRANK CHRISTOPHER ◽  
CARL VANDERZANT
Keyword(s):  
Storage Temperature ◽  
Penaeid Shrimp ◽  
Enzymic Activity ◽  
White Shrimp ◽  
Short Term ◽  
Color Development ◽  
Red Color ◽  
Penaeus Setiferus ◽  
Urea Production ◽  
Orange Color

White shrimp (Penaeus setiferus) were held at 0, 10, 20, 30, 37, and 44 C for 3, 6, and 24 h. Serious quality deterioration, as evidenced by off-color development (red and orange pigmentation) and off-odor development, was beginning to occur in shrimp held for 3 h at 30, 37, and 44 C, for 6 h at 20 C and for 24 h at 10 C. Red color development was evident in shrimp held at 30 and 37 C, orange color in those held at 44 C. Putrid odors appeared more rapidly in shrimp held at 37 than at 44 C where shrimp developed cooked-shrimp odors. Large increases in bacterial counts at 30–44 C (after 6 and 24 h) were usually accompanied by putrid odors. Tissue pH changes were erratic and small. Total volatile nitrogen (TVN), free amino acid nitrogen (AA-N), and urea production increased with storage temperature during the 3- and 6-h storage experiments. Musty and cooked-shrimp off-odors developed in the shrimp as a result of chemical and/or enzymic activity while putrid and sour odors were produced by bacteria growing in the shrimp.

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