The Superior Outcome of Collagen Nanosilver in Increasing Macrophage and Collagen on Deep Dermal Burn Wound Healing

AIM: This study aims to prove the superior outcome of collagen nanosilver in increasing macrophage and collagen on deep dermal burn wound healing. METHODS: This study was a randomized control trial design. All animals used were male Sprague Dawley rats aged 12–14 weeks old and weighed about 200–250 g. Each rat gets four site injuries and is given treatments. The treatment group includes: I (negative control using NaCl 0.9%), II (positive control using silver sulfadiazine cream), III (treatment with nanosilver collagen cream 1%), and IV (treatment with collagen nanosilver cream 2%). Macrophages counts were assessed using IHC F40/80 and collagen density was assessed using Masson’s trichrome staining. RESULTS: Macrophage on day 6 showed a significant difference as seen in group III and group IV. A significant difference was also obtained between macrophage on day 3 and day 6 from group IV, likewise between collagen between day 3 and day 6 from group III and IV. Collagen nanosilver acts as an antimicrobial and anti-inflammatory agent by increasing the mechanism of activation and migration of macrophages to the site of injury. Moreover, this formula modulated the collagen deposition, which forms the tissue fibers which ultimately provide a conducive environment for wound healing. CONCLUSION: Collagen nanosilver has a superior outcome in the increasing macrophage cell count and collagen density of deep dermal burn wound healing.

Download Full-text

Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.9613 ◽

2019 ◽

Vol 5 (2) ◽

pp. 17

Author(s):

Yuli Hermansyah ◽

Ika Aulia Kurniasari ◽

Heni Fatmawati

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

Antioxidant Properties ◽

Negative Control ◽

Silver Sulfadiazine ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Significant Difference

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

Download Full-text

Evaluation of the Healing Effects of Hypericum perforatum and Curcumin on Burn Wounds in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6462956 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Nevra Seyhan

Keyword(s):

Wound Healing ◽

Hypericum Perforatum ◽

Control Group ◽

Sprague Dawley ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Group A ◽

Second Degree

Background. For centuries, medicinal plants have been extensively used in wound healing of burn injuries. The aim of this study is to analyze comparatively the effects of curcumin and Hypericum perforatum (HP) on second-degree burn wounds in rats. Materials and Methods. This experimental study was conducted on 24 male Sprague-Dawley rats with second-degree burns. The animals were randomly divided into three groups. The burns were treated with curcumin (Group B) and Hypericum perforatum (Group C) with daily application. Group A was considered as the control group and received no medication. Histological parameters, reepithelization, granulation tissue formation, inflammation, and angiogenesis were assessed after the scar biopsy at the end of research. Results. All histological parameters of the control group showed statistically significant difference than the other groups (p<0.05). There was no statistically significant difference between Groups B and C in terms of reepithelization and inflammation (p=0.351, p=0.067). In the curcumin group compared with the HP group, a significant difference was observed for two parameters of fibrosis and angiogenesis (p<0.05). Conclusion. Both curcumin and Hypericum perforatum oil are effective in burn wound healing. Our findings showed a better quality of healing in curcumin-treated rats.

Download Full-text

PENGARUH PEMBERIAN EKSTRAK PROPOLIS LEBAH TANPA SENGAT PADA PENYEMBUHAN LUKA BAKAR TIKUS PUTIH (Rattus norvegicus)

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v8i1.4585 ◽

2021 ◽

Vol 8 (1) ◽

pp. 68-76

Author(s):

Nelky Suriawanto ◽

Evi Setyawati ◽

Narwan

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Stingless Bee ◽

Control Group ◽

Burn Wound ◽

Propolis Extract ◽

Burn Wound Healing

The Effect of Treatment Using Stingless Bee Propolis Extract on Burn Wound Healing in Rattus norvegicus Burn would occurs as a result of direct or indirect fire burns, exposure to sunlight and chemicals. This study aims to analyze the effect of stingless bee propolis extract (Tetragonula fuscobalteata) on the burn wound healing of the back skin of Rattus norvegicus. An experimental research was carried out using the control and experimental groups. Twenty five R. norvegicus were divided into 5 groups: the negative control group was given 70% ethanol, the positive control group was given Bioplacenton® and 3 groups were treated with propolis extract with a concentration of 50%, 70% and 100%. Measurement of burn diameter was carried out every 2 days for 21 days. Data were analyzed statistically using the one way Anova method. The results of phytochemical screening showed that the stingless bee propolis extract contained flavonoids, phenolics, tannins and saponins. The test of the effectiveness of propolis extract against burn wound obtained a sig value of 0.00 (< 0.05). stingless bee propolis extract could heal burn wound in R. norvegicus. Luka bakar dapat terjadi akibat terbakar api secara langsung atau tidak langsung, paparan sinar matahari dan bahan kimia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak propolis lebah tanpa sengat (Tetragonula fuscobalteata) terhadap penyembuhan luka bakar pada kulit punggung Rattus norvegicus. Metode yang digunakan merupakan penelitian true experimental menggunakan kelompok kontrol dan eksperimen. Dua puluh lima ekor R. norvegicus dibagi menjadi 5 kelompok: kelompok kontrol negatif diberi etanol 70%, kontrol positif diberi Bioplacenton®, dan 3 kelompok perlakuan ekstrak propolis dengan konsentrasi 50%, 70%, dan 100%. Pengukuran diameter luka bakar dilakukan setiap 2 hari selama 21 hari. Data dianalisa secara statistik menggunakan metode Anova satu arah. Hasil skrining fitokimia ekstrak propolis lebah tanpa sengat positif mengandung senyawa flavonoid, fenolik, tanin, dan saponin. Uji efektivitas ekstrak propolis terhadap luka bakar didapatkan nilai sig 0,00 (< 0,05). Ekstrak propolis lebah tanpa sengat dapat menyembuhkan luka bakar pada R. norvegicus.

Download Full-text

MANFAAT EKSTRAK ETANOL DAUN REMEK DAGING (Hemigraphis colorata W. Bull) TERHADAP LUKA BAKAR PADA TIKUS

Jurnal Tumbuhan Obat Indonesia ◽

10.22435/jtoi.v13i1.2823 ◽

2020 ◽

Vol 13 (1) ◽

pp. 32-41

Author(s):

Lusi Putri Dwita ◽

Vera Ladeska ◽

Aisyah Ramadhani ◽

Dwi Rahma Augusta ◽

Retno Tri Saufia

Keyword(s):

Wound Healing ◽

Topical Application ◽

Ethanolic Extract ◽

Positive Control ◽

Negative Control ◽

Silver Sulfadiazine ◽

Burn Wound ◽

Burn Wounds ◽

Wound Induction ◽

Collagen Density

ABSTRACT Remek daging (Hemigraphis colorata W.Bull) have been studied and used traditionally for wound healing. This study aimed to determine the effect of topical application of remek daging leaves ethanolic extract 70% on the burn wound. The animals used for this study were 30 rats, divided into five groups, namely 20, 10, 5% remek daging extract ointment, negative control (vaseline flavum), and positive control (silver sulfadiazine 1%). Histology observations were held on days 3, 7, and 14 after burn wound induction. Histological observations showed an increase number of macrophages, fibroblasts, collagen density, and re-epithelialization in the extract ointment group significantly compare to the negative control (p <0.05). The application of ointment extract 20% to the rats showed comparable results to silver sulfadiazine 1% (p> 0.05). It can be concluded that remek daging ointment extract can accelerate the healing of burn wounds with the best results at a concentration of 20%. Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen. ABSTRAK Remek daging (Hemigraphis colorata W.Bull) telah diteliti dan digunakan untuk penyembuhan luka secara tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 70% daun remek daging secara topikal pada luka bakar tikus putih. Hewan yang digunakan adalah 30 ekor tikus yang dibagi menjadi 5 kelompok yaitu kelompok salep ekstrak daun remek daging 20, 10, 5% (%b/b), kontrol negatif (vaselin flavum) dan kontrol positif (silver sulfadiazine 1% (%b/b)). Pengamatan secara histologi dilakukan pada hari ke 3, 7 dan 14 setelah induksi luka bakar. Pengamatan histologi menunjukkan peningkatan jumlah makrofag, jumlah fibroblas, kepadatan kolagen dan ketebalan re-epitelisasi pada kelompok salep ekstrak daun remek daging secara signifikan dibandingkan kontrol negatif (p<0,05). Tikus yang diberikan perlakuan salep ekstrak 20% menunjukkan hasil sebanding dengan silver sulfadiazin 1% (p>0,05). Dapat disimpulkan bahwa salep ekstrak daun remek daging dapat mempercepat penyembuhan luka bakar dengan hasil terbaik pada konsentrasi 20%. Kata kunci: Hemigraphis colorata, luka bakar, makrofag, fibroblas, kolagen.

Download Full-text

OINTMENT OF Eupatorium odoratum L. EXTRACT PROMOTES BURN WOUND HEALING IN MALE ALBINO MICE

SCIENTIA : Jurnal Farmasi dan Kesehatan ◽

10.36434/scientia.v4i1.71 ◽

2016 ◽

Vol 4 (1) ◽

pp. 1

Author(s):

Eka Fitrianda ◽

Wida Ningsih

Keyword(s):

Wound Healing ◽

Statistical Analysis ◽

Reference Group ◽

Control Group ◽

Burn Wound ◽

Group Iii ◽

Burn Wound Healing ◽

Group I ◽

Significant Difference ◽

Ointment Base

Eupatorium odoratum L. is traditionally used to treat open wounds. Aim of this study was toinvestigate the healing activity of ointment containing extract of E. odoratum in burns induced in malealbino mice. Mice were divided into 5 groups, all were induced for burn wound using a heat stamp intemperature 80°C for 20 minutes. Group I was treated with ointment base (control), group II, III and IVwere treated with extract ointment in concentration of 5%, 10%, and 20% w/w respectively, and the lastgroup V was treated with ointment reference. Observations were made during 21 days exactly on the 7th,14th and 21st day, which included parameters: percentage of healed area, epithelialization time andcollagen scores. The result showed that on 14th day, mean of healed area in group III (75.89%±7.76%)and IV (76.29%±6.981%) were significantly higher than other groups (P<0.05). On the 14th and 21th day,collagen scores in groups III (2.33±0.577 and 2.67±0.577) and IV (2.67±0.577 and 2.33±0.577) are equalwith reference group, these score are significantly higher than in groups I and II (P<0.05). Meanwhile,though epithelialization time in group III and IV (both 14.33 days±0.577days) are more quickly thanothers, statistical analysis showed that there is no significant difference on time between all groups(P>0.05).

Download Full-text

The Effectiveness of Edamame Seed (Glycine max L. Merril) Ethanolic Extract to Fibroblast Count on Second Degree Burn Wound Healing

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i3.6831 ◽

2019 ◽

Vol 5 (3) ◽

pp. 154

Author(s):

Arifah Nur Hasanah ◽

Ika Rahmawati Sutejo ◽

Enny Suswati

Keyword(s):

Wound Healing ◽

Antioxidant Properties ◽

Ethanolic Extract ◽

Negative Control ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The study was conducted at University of Jember during November until December 2017. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. The number of fibroblast of each picture was processed using Image-J software. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

Download Full-text

Pengujian Efektivitas Penyembuhan Luka Mencit Diabetes Melitus Yang Diberikan Sediaan Krim Ekstrak Etanol Daun Bandotan

SCIENTIA : Jurnal Farmasi dan Kesehatan ◽

10.36434/scientia.v6i1.42 ◽

2016 ◽

Vol 6 (1) ◽

pp. 50

Author(s):

Ria Afrianti ◽

Dedi Nofiandi ◽

Dira Dira ◽

Widya Ulfa

Keyword(s):

Wound Healing ◽

Ethanolic Extract ◽

Positive Control ◽

Negative Control ◽

Collagen Fibers ◽

Group Iv ◽

Ageratum Conyzoides ◽

Group V ◽

Group Iii ◽

Group I

Bandotan plants (Ageratum conyzoides (L.) L.) commonly used as agent in the treatment of inflammatory, wound, and also used used as analgesic, stimulant and tonic. In this study we carried out a test of the effectiveness of the cream (w/o) of ethanolic extract of bandotan leaves in wound healing in streptozocin-induced hyperglycemia mice. Animals were divided into five groups: Group I (without induced, given cream base w/o), group II (induced, given cream base w/o), group III (induced, given cream containing 3% extract), group IV (induced, given cream containing 5% extract) and group V (induced, given cream containing 7% extract). Animals also divided into three subgroups based on time of decapitation on 7th, 14th and 21th days. The parameters observed were the percentage of wound healing and collagen fibers density scores. Results were analyzed by Two-Way ANOVA. The results showed that the cream in concentration of 3%, 5% and 7% could affect wound healing in hyperglycemia mice. The percentage of wound healing of the cream in concentration of 3%, 5% and 7% were significantly different (p<0,05) with positive control, and density scores of collagen fibers was also significantly different (p<0,05) from the positive control and negative control. However, the best results was obtained from cream containing 5% extract which perrcentage of wound healing reached 98.5% on 21th day and got a score of 3 for density of collagen fibers that had been formed on the 14th day.

Download Full-text

PENGARUH INFUSA DAUN KEMBANG BULAN (Tithonia difersifolia) PADA KECEPATAN PENYEMBUHAN LUKA BAKAR

Jurnal IPTEKS Terapan ◽

10.22216/jit.2018.v12i3.583 ◽

2018 ◽

Vol 12 (3) ◽

pp. 230

Author(s):

Dwi Mulyani

Keyword(s):

Wound Healing ◽

Positive Control ◽

Negative Control ◽

Full Thickness ◽

Sprague Dawley ◽

Burn Wound ◽

Rat Models ◽

Sprague Dawley Rats ◽

Healing Wound ◽

Heated Metal

The aim of this investigation was a clinical evaluation of the accelerating effect of Tithonia difersifolia extract on the burn <a title="Find more articles at http://www.scialert.net/asci/result.php?searchin=Keywords&cat=&ascicat=ALL&Submit=Search&keyword=wound+healing (wound healing)" href="http://www.scialert.net/asci/result.php?searchin=Keywords&cat=&ascicat=ALL&Submit=Search&keyword=wound+healing" target="_blank">wound healing</a> in male Sprague-Dawley rats weighted 200-350. The sample then extracted using wet rendering (infusa) method using aqua-dest as extractor. The subjects were nine male Sprague-Dawley rats devided in to three group (negative control, positive control and experimental). Every group consist of three rats. Heated metal plat (d=2cm) used to get full thickness burn wound on dorsal rat part. Then, Tithonia difersifolia folium extract was given to wound healing(plannimetri method) for 14 days, negative control(aqua dest) 26 days and positive control (Bioplasenton) 20 days. The results with analysis Anova showed that Tithonia difersifolia folium extract have significant effect (p < 0,05) to wound healing of rat models.

Download Full-text

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

Download Full-text

Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

10.1101/436444 ◽

2018 ◽

Author(s):

Yasuhiko Kaita ◽

Takehiko Tarui ◽

Hideaki Yoshino ◽

Takeaki Matsuda ◽

Yoshihiro Yamaguchi ◽

...

Keyword(s):

Wound Healing ◽

Therapeutic Effect ◽

Healing Process ◽

Control Group ◽

Type I ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

Download Full-text