scholarly journals Systematic identification of human housekeeping genes possibly useful as references in gene expression studies

2017 ◽  
Vol 16 (3) ◽  
pp. 2397-2410 ◽  
Author(s):  
Maria Caracausi ◽  
Allison Piovesan ◽  
Francesca Antonaros ◽  
Pierluigi Strippoli ◽  
Lorenza Vitale ◽  
...  
Keyword(s):  
Gene Expression ◽  
Housekeeping Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Systematic Identification

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

Validation of housekeeping genes as an internal control for gene expression studies in the brain of ovariectomized rats treated with tibolone

Gene ◽  
2021 ◽  
Vol 769 ◽  
pp. 145255
Author(s):  
Iris A. Feria-Romero ◽  
Iván Bribiesca-Cruz ◽  
Angélica Coyoy-Salgado ◽  
Julia J. Segura-Uribe ◽  
Guadalupe Bautista-Poblet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Internal Control ◽  
Housekeeping Genes ◽  
Ovariectomized Rats ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
The Brain

scholarly journals Gene expression studies in different genotypes of an ectomycorrhizal fungus require a high number of reliable reference genes

2016 ◽  
Author(s):  
Joske Ruytinx ◽  
Tony Remans ◽  
Jan V Colpaert
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Intraspecific Variability ◽  
Standard Technique ◽  
Housekeeping Genes ◽  
Ectomycorrhizal Fungus ◽  
Suillus Luteus ◽  
Qrt Pcr ◽  
Expression Studies ◽  
Gene Expression Studies

Quantitative reverse transcription PCR (qRT-PCR) has become the standard technique for the expression analysis of a set of chosen genes of interest. The accuracy and reliability of qRT-PCR measurements strongly depends on the normalization with appropriate endogenous reference genes. In this study a set of candidate reference genes for the use in gene expression studies of a basidiomycete fungus, Suillus luteus, exposed to toxic concentrations of zinc or cadmium was identified, evaluated and validated. Seven candidate genes were selected from cDNA-AFLP as stably expressed and the algorithms geNorm and Normfinder were used to evaluate these genes alongside the traditionally used housekeeping genes (actin, tubulin) in different S. luteus isolates. The use of several S. luteus isolates revealed that each isolate has its own most stably expressed set of reference genes, regardless of the metal treatments, in casu metal exposures. Metal treatments had only a minor impact on the expression of the candidate reference genes. The validated reference genes outperform the in fungal research commonly used single, arbitrary chosen (“housekeeping”) genes in terms of reliability, and have the potential to be suitable reference genes when studying the effect of other environmental factors. A relatively high number of reference genes is required to correct for intraspecific variability when studying natural populations.

Validation of Housekeeping Genes for Gene Expression Studies in Symbiodinium Exposed to Thermal and Light Stress

2010 ◽  
Vol 13 (3) ◽  
pp. 355-365 ◽  
Author(s):  
Nedeljka N. Rosic ◽  
Mathieu Pernice ◽  
Mauricio Rodriguez-Lanetty ◽  
Ove Hoegh-Guldberg
Keyword(s):  
Gene Expression ◽  
Light Stress ◽  
Housekeeping Genes ◽  
Expression Studies ◽  
Gene Expression Studies

Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation

Extremophiles ◽  
2012 ◽  
Vol 16 (3) ◽  
pp. 419-425 ◽  
Author(s):  
Chenlin Liu ◽  
Guangting Wu ◽  
Xiaohang Huang ◽  
Shenghao Liu ◽  
Bailin Cong
Keyword(s):  
Gene Expression ◽  
Housekeeping Genes ◽  
Expression Studies ◽  
Freezing Acclimation ◽  
Gene Expression Studies

Selection of Housekeeping Genes for Gene Expression Studies in a Rat Model of Irinotecan-Induced Mucositis

Chemotherapy ◽  
2011 ◽  
Vol 57 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Noor Al-Dasooqi ◽  
Joanne M. Bowen ◽  
Rachel J. Gibson ◽  
Richard M. Logan ◽  
Andrea M. Stringer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Model ◽  
Housekeeping Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Establishment of Optimal Housekeeping Genes for Urinary Extracellular Vesicle Biomarker Development: A Step Towards Non-Invasive Diagnostics

2021 ◽  
Author(s):  
Anula Divyash Singh ◽  
Rajeswari Koyyada ◽  
Rasmita Samal ◽  
Syed Baseeruddin Alvi ◽  
Sreekanth Patnam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quantitative Polymerase Chain Reaction ◽  
Housekeeping Genes ◽  
Extracellular Vesicle ◽  
Distribution Profile ◽  
Non Invasive ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
Invasive Diagnostics

Abstract Background. Urinary extracellular vesicles (UEVs) hold RNAs and can find their application in multiplex biomarker development. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the most commonly used method for gene expression studies. However, there are no reported optimal housekeeping genes available to normalize qPCR data for the UEVs RNA pool.Methods. UEVs were precipitated by Polyethylene glycol (P.E.G. Mn6000) from the urine of 40 human individuals and characterized for their molecular, biophysical, and biochemical purity and integrity. In addition, the expression of five commonly used housekeeping genes B2M, RPL13A, PPIA, HMBS, and GAPDH, were quantified by qPCR in the UEV RNA pool and analyzed through algorithms of NormFinder, geNorm, BestKeeper, and Delta Ct integrated into ReFinder.Results: 12% PEG precipitation yielded round and cup-shaped UEVs. The size and distribution profile of UEVs were around 30 – 100nm through electron microscopy, NTA, and DLS. Acetylcholine esterase and Dipeptidyl peptidase-IV activity were used to arrive at their functional purity. B2M and RPL13A genes were identified as stable genes with a mean stability score of 1.5(geNorm) and below 1 (NormFinder) through ReFinder, which yield comprehensive ranking analysisConclusions: B2M and RPL13A are optimal reference genes and can be used for UEVs based gene expression studies.

Sign in / Sign up

Export Citation Format

Share Document