scholarly journals A natural bacterial strain Bacillus pumilus 16: Identification and antibiotic resistance evaluation

2021 ◽  
Vol 7 ◽  
pp. 391-406
Author(s):  
Alena N. Irkitova ◽  
Angelina V Malkova ◽  
Dina E. Dudnik
Keyword(s):  
Antibiotic Sensitivity ◽  
World Market ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
E Coli ◽  
Natural Strain ◽  
Highly Sensitive ◽  
Type Strains

Microbial biopreparations are actively used to prevent, diagnose, and treat infectious, allergic, tumor, and autoimmune diseases in humans and animals; to stimulate the growth and development of plant crops. Natural bacterial strains with valuable technical properties are a vital biological resource for developing new biopreparations and rotating already known microbial preparations in the world market. This study describes a new natural strain B. pumilus 16, which was isolated from the rhizosphere of Cichorium. The strain was identified using morphological and physiological parameters, biochemical tests, and primers Pum-f. and Pum-r. Antibiotic sensitivity and antagonistic activity against Escherichia coli were determined by diffusion of discs and delayed antagonism methods, respectively. The new natural strain (like type strains) fermented arabinose, cellobiose, mannitol, mannose, salicin, sucrose, and trehalose, and gave a positive reaction to arginine dihydrolase, ONPG, Voges-Proskauer test. It also gave a negative reaction to inositol, raffinose, sorbitol, methyl-D-glucoside, inulin, and lecithinase. B. pumilus 16, unlike the test strains, was capable of fermenting citrate. Strain B. pumilus 16 was highly sensitive to cephalexin (37.9±0.7 mm) and enrofloxacin (25.7±8.9 mm); sensitive to ole-andomycin (17.1±1.9 mm), benzylpenicillin (18.5±1.2 mm), and monomycin (16.0±0.6 mm); resist-ant to oxacillin. By the agar blocks method (7.3±1.5 mm), a more pronounced antagonism of the new strain against E. coli was recorded than by the method of agar wells (5.3±0.6 mm). Due to the level of antagonistic activity, B. pumilus 16 was more effective than the type strains (two of which did not show an antagonistic effect). On the basis of this, the new strain can be recommended for inclusion in the bacterial preparation composition for the national economy.

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

scholarly journals Blood Culture Isolates and Antibiogram of Salmonella: The Gathering Storm

KYAMC Journal ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 188-190
Author(s):  
Ishrat Binte Reza ◽  
HAM Nazmul Ahasan ◽  
Moshtaque Ahmed ◽  
Homayra Tahseen ◽  
Tasmina Chowdhury
Keyword(s):  
Drug Resistance ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
College Hospital ◽  
Major Health ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
The World

Introduction: Typhoid fever is a common problem all over the world including Bangladesh. It is caused by salmonella typhae and paratyphae and it is an old infectious water born disease. It is encountered in Bangladesh throughout the year. For many years it is treated by Chloramphenicol and subsequently Cotrimoxazole and Amoxicillin. Despite the use of newly developed antibacterial drugs, enteric fevers caused by multidrug-resistant bacterial strains are one of major health problems in Bangladesh. Multidrug resistant Salmonella sp. has been emerged is a cause of concern. Materials and Methods: This is a retrospective study done at the Popular Medical College Hospital over a period of 6 months between January 2018 to june 2018. Results: During the study period, total 115 cases were enrolled. Regarding antibiotic sensitivity pattern, 69% strains were sensitive to Ampicillin, 100% to Cefixime and Ceftriaxone, 75% to Cotrimoxazole, 52% to Nalidixic Acid and 68% to Ciprofloxacin, 69% to Levofloxacin, 80% to Cholarphenicol. Conclusion: Ceftriaxone and Cefixime were found to be highly sensitive. So, we should be very careful for the judicious use of these valuable drug to prevent drug resistance. KYAMC Journal Vol. 10, No.-4, January 2020, Page 188-190

scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

scholarly journals Antibiogram and recent incidence of multi-drug resistant carbapenemase producing Escherichia coli isolated from paediatric patients

2019 ◽  
Vol 36 (2) ◽  
Author(s):  
Sumbal Nosheen ◽  
Nadeem Irfan Bukhari ◽  
Hasan Ejaz ◽  
Nasir Abbas
Keyword(s):  
Escherichia Coli ◽  
Carbapenem Resistance ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
E Coli ◽  
Creative Commons ◽  
Paediatric Patients ◽  
Diffusion Technique ◽  
Open Access Article

Objective: To gauge the recent breadth of MDR E. coli along with antibiogram of carbapenemase producing (CP) E. coli among children from an institute which receives patients from all over Punjab. Methods: The bacterial strains of E. coli isolated from various specimens of patients were collected from April 2017 to August 2018 and processed using standard biochemical tests and API 20E system (bioMerieux). Phenotypic screening for CP E. coli was done by the modified Hodge test, whereas antibiotic susceptibility testing was done with Kirby-Bauer disc diffusion technique. Results: Total of 6,468 bacterial strains were isolated, out of which 1,552 (24%) were E. coli. Carbapenem resistance was observed in 245 (16%) strains, amongst which 113 (46%) were confirmed to be CP. E. coli isolated from males were higher as compared to females (p<0.05). Majority of the organisms were isolated from blood (37.2%) samples. The hospital discharged about 65% of patients, while 23% left against medical advice. Overall MDR amongst E. coli was 93.26%. Colistin sulphate (15.9%) and nitrofurantoin (16.8%) showed the most efficacy followed by amikacin (15%) and fosfomycin (10.6%). Conclusion: The isolation of high number of MDR E. coli amongst the paediatric patients is worrisome, which could serve as a potential source of horizontal genes transfer to other genera. doi: https://doi.org/10.12669/pjms.36.2.928 How to cite this:Nosheen S, Bukhari NI, Ejaz H, Abbas N. Antibiogram and recent incidence of multi-drug resistant carbapenemase producing Escherichia coli isolated from paediatric patients. Pak J Med Sci. 2020;36(2):---------. doi: https://doi.org/10.12669/pjms.36.2.928 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

scholarly journals Evaluation of Phenotypic Variations in the Antibiotics Sensitivity of Escherichia Coli by Repeated Exposure

2021 ◽  
pp. 6-11
Author(s):  
Andry Maharo Andrianarivelo ◽  
Christian Emmanuel Mahavy ◽  
Marson Raherimandimby ◽  
Tsiry Rasamiravaka
Keyword(s):  
Escherichia Coli ◽  
Bacterial Resistance ◽  
Antibiotic Sensitivity ◽  
Repeated Exposure ◽  
University Hospital ◽  
Bacterial Strains ◽  
E Coli ◽  
Sensitivity Tests ◽  
Excessive Intake ◽  
Antibiotics Sensitivity

Enterobacteriaceae, in particular Escherichia coli, are habitual residents of the gastrointestinal tract, capable of causing a large number of infections. The MIC varies according to the bacterial strains and the antibiotics used, hence the need to carry out antibiotic sensitivity tests. The objective of this study is to evaluate the behavior of Escherichia coli after repeated exposure to the same antibiotic to demonstrate a possible correlation between excessive intake of antibiotics and bacterial resistance. A prospective and descriptive study was carried out in the Laboratory of Microbiology of Fundamental and Applied Biochemistry (Faculty of Sciences Antananarivo) during the month of November 2019. The strains studied were the reference strain Escherichia coli ATCC 25922 provided by the Laboratory and two clinical strains from the Microbiology Laboratory of the Joseph Ravoahangy Andrianavalona University Hospital Center (CHU JRA) Antananarivo. Repeated exposure to Tobramycin and Ofloxacin of these strains were performed. The results of our study showed that most E. coli is exposed to the antibiotic, the more it develops resistance. The evolution of E. coli's sensitivity is different in the presence of Tobramycin with MICs up to 4 times the starting value while in the presence of Ofloxacin, the MIC increases to 125 times the initial value. This difference may be due to the different target of the antibiotic which causes the bacteria to develop variable mechanisms to escape it. Key words: E. coli - MIC - antibiotics - repeated exposure

scholarly journals Arthrobacter livingstonensis sp. nov. and Arthrobacter cryotolerans sp. nov., salt-tolerant and psychrotolerant species from Antarctic soil

2011 ◽  
Vol 61 (4) ◽  
pp. 979-984 ◽  
Author(s):  
Lars Ganzert ◽  
Felizitas Bajerski ◽  
Kai Mangelsdorf ◽  
André Lipski ◽  
Dirk Wagner
Keyword(s):  
Type Strain ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Cold Tolerant ◽  
Respective Type ◽  
Ph Range ◽  
Type Strains ◽  
Physiological And Biochemical

Two novel cold-tolerant, Gram-stain-positive, motile, facultatively anaerobic bacterial strains, LI2T and LI3T, were isolated from moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St Kliment Ohridski. A rod–coccus cycle was observed for both strains. 16S rRNA gene sequence analysis revealed an affiliation to the genus Arthrobacter, with the highest similarity to Arthrobacter stackebrandtii and Arthrobacter psychrochitiniphilus for strain LI2T (97.8 and 97.7 % similarity to the respective type strains) and to Arthrobacter kerguelensis and Arthrobacter psychrophenolicus for strain LI3T (97.4 and 97.3 % similarity to the respective type strains). The growth temperature range was −6 to 28 °C for LI2T and −6 to 24 °C for LI3T, with an optimum at 16 °C for both strains. Growth occurred at 0–10 % (w/v) NaCl, with optimum growth at 0–1 % (w/v) for LI2T and 0.5–3 % (w/v) for LI3T. The pH range for growth was pH 4–9.5 with an optimum of pH 8 for LI2T and pH 6.5 for LI3T. The predominant fatty acids were anteiso-C15 : 0, C18 : 0 and anteiso-C17 : 0 for LI2T and anteiso-C15 : 0 and C18 : 0 for LI3T. Physiological and biochemical tests clearly differentiated strain LI2T from A. stackebrandtii and A. psychrochitiniphilus and strain LI3T from A. kerguelensis and A. psychrophenolicus. Therefore, two novel species within the genus Arthrobacter are proposed: Arthrobacter livingstonensis sp. nov. (type strain LI2T  = DSM 22825T  = NCCB 100314T) and Arthrobacter cryotolerans sp. nov. (type strain LI3T  = DSM 22826T  = NCCB 100315T).

scholarly journals PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments

2015 ◽  
Vol 3 (6) ◽  
pp. 361 ◽  
Author(s):  
Homaira Anzum Himi ◽  
Md. Shafiullah Parvej ◽  
M. Bahanur Rahman ◽  
K M Nasiruddin ◽  
Wahedul Karim Ansari ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Foodborne Pathogen ◽  
Antibiotic Sensitivity ◽  
Cloacal Swab ◽  
Poultry Feed ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
E Coli ◽  
Source Of Infection ◽  
Stx1 Gene

Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. coli in poultry, detection of its source of infection in poultry and transmission pattern to human. For this purpose a total of 150 samples (cloacal swab-60, feed -15, water-15 and egg -60) were collected and analyzed in bacteriology laboratory by cultured in different bacteriological media followed by gram’s staining, biochemical tests and Polymerase Chain reaction (PCR). The PCR was performed by targeting 16s rRNA gene and shiga toxin producing gene in E. coli. Out of 150 collected samples, E. coli was found in 81 (54%) samples. Presence of E. coli was 100% in both feed (n=15) and egg (n=60), whereas 10% in cloacal swab (n=6). Water samples were totally free of E. coli. The stx2 gene was detected in all samples whether all samples were negative for stx1 gene. The study revealed that, poultry feed acts as a source of E. coli infection in poultry, which may be transmitted to environment and human via meat or eggs. Antibiotic sensitivity test revealed that isolated bacteria were highly sensitive to Ciprofloxacin.

scholarly journals Haemato-biochemical Changes and Antibiotic Sensitivity to Eschericia coli Associated with Concurrent Enteric and Septicaemic Infection in Calves

1970 ◽  
Vol 1 (1) ◽  
pp. 39-43 ◽  
Author(s):  
MA Samad ◽  
MA Islam ◽  
KA Hossain ◽  
MT Islam ◽  
S Saha
Keyword(s):  
Escherichia Coli ◽  
Serum Magnesium ◽  
Antibiotic Sensitivity ◽  
Blood Samples ◽  
E Coli ◽  
Drug Of Choice ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Escherichia Coli Infection ◽  
First Time

Concurrent enteric and septicaemic Escherichia coli infection was recorded in six local calves aged between 8 to 12 months old for the first time in Bangladesh during the period from May to June 2002. Bacteriologic examination of both the faecal and blood samples of six febrile calves showed positive for E. coli of all the six faecal (100%) and only two blood (33.33%) samples. Clinical examination revealed significantly (p < 0.01) high rectal temperature at peak febrile stage which varied from 103.5 to 106°F with a mean of 104.7 ± 0.85°F. The fever associated signs and clinical anaemia were recorded in all these calves. Haematological examination showed significantly (p < 0.01) decreased haemoglobin (5.58 ± 0.45 g%), packed cell volume (19.83 ± 1.95 %), total erythrocytic count (3.52 ± 0.58  106/ mm3) and total leukocytic count (6.68 ± 0.44  103 / mm3) which indicates anaemia and leukopenia at the peak febrile stage of the disease. Biochemical studies showed significantly (p < 0.05) increased that serum magnesium and iron values were at fever stage, whereas serum phosphorus significantly (p < 0.01) decreased at the 4th day (convalescent stage) but no significant (p > 0.05) change was observed with the serum calcium, sodium, potassium and chloride values between prefever and fever stage. Antibiotic sensitivity pattern of E. coli isolated from faecal and blood samples revealed that these isolates were found to be resistant to penicillin, ampicillin and amoxycillin, moderately sensitive to gentamicin and streptomycin but highly sensitive to tetracycline. This indicates that tetracycline could be the drug of choice for both the septicaemic and enteric E. coli infection in calves. Key words: Haematobiochemical; Escherichia coli; enteric; septicemic; infection; calves DOI = 10.3329/bjvm.v1i1.1916 Bangl. J. Vet. Med.  (2003). 1(1) : 39 - 43

Sign in / Sign up

Export Citation Format

Share Document