scholarly journals The effect of intranasal administration of an IL-1b antagonist (RAIL) on the state of the nitroxydergic system of the brain during modeling of acute cerebrovascular accident

Pharmacia ◽  
2021 ◽  
Vol 68 (3) ◽  
pp. 665-670
Author(s):  
Bogdan S. Burlaka ◽  
Igor F. Belenichev ◽  
Olga I. Ryzhenko ◽  
Victor P. Ryzhenko ◽  
Olena G. Aliyeva ◽  
...  
Keyword(s):  
Cerebrovascular Accident ◽  
Nitrosative Stress ◽  
The State ◽  
Inos Mrna ◽  
Rt Pcr ◽  
Ischemic Brain ◽  
Acute Cerebrovascular Accident ◽  
Full Effect ◽  
Polymerase Chain ◽  
The Brain

Introduction: This study was designed to evaluate the effect activity of RAIL-gel in comparison with Citicoline on nitroxydergic system during acute cerebrovascular accident. Methods: In this study, 80 white nonlinear rats were randomly assigned to 4 groups (20 rats in each): 1) intact; 2) control - untreated with acute cerebrovascular accident (ACVA), examined on the 4th day; 3) animals with ACVA, receiving RAIL, examined on the 18th day; 4) animals with ACVA, treated with Citicoline, examined on the 4th day. The expression of inducible NOS was determined by Western blotting. The nitrosative stress marker, nitrotyrosine, was determined using the ELISE kit NITROTYROSINE (kit no. HK501-02, series 12825k1212-k). To assess the state of iNOS mRNA expression, we used the method of polymerase chain reaction with reverse transcription in real time (RT-PCR). Results: Our research demonstrated that course administration of the RAIL and Citicoline to animals with ACVA for 4 days leads to the stabilization of the parameters of the brain nitroxydergic system. However, Citicoline does not provide a full effect on the shifts of the NO system in the brain. It does not have the proper effect on such an important link in the pathogenesis of ischemic brain damage as nitrosative stress. RAIL leads to a significant decrease in NOS activity due to its inducible form (decrease in the expression of iNOS and iNOS mRNA) and a decrease in NO metabolits, and inhibition of nitrosative stress (decrease in nitrotyrosine). Conclusion: IL-1b antagonist RAIL (Intranal Gel) significantly exceeds Citicoline in terms of the severity of the effect on the nitroxydergic system indicators.

Assessment of the Willis circle pathology in patients with acute cerebrovascular accident

2020 ◽  
Vol 18 (5) ◽  
pp. 99-104
Author(s):  
S. K. PERMINOVA ◽  
◽  
A. A. YAKUPOVA ◽  
A. Ya. NAZIPOVA ◽  
R. T. GAYFUTDINOV ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Neurological Deficit ◽  
Cerebrovascular Accident ◽  
National Institutes Of Health ◽  
Stroke Severity ◽  
Functional Disorders ◽  
Good Recovery ◽  
Acute Cerebrovascular Accident ◽  
The Brain

The purpose — to evaluate the Willis circle pathology in patients with acute cerebral circulatory disorders using the National Institutes of Health Stroke Scale, to assess the degree of functional disorders and the long-term perspective. Material and methods. Data from magnetic resonance imaging of the brain and magnetic resonance angiography of the brain were analyzed in 47 patients with acute cerebrovascular accident. The study included 21 (44,6%) men and 26 (55,4%) women, with an average age of 67,08 ± 16,03. All patients underwent neurological examination using the National Institutes of Health Stroke Scale, assessment of functional disorders using the Rivermead scale, and assessment of long-term perspective using the Rankin scale before and after treatment. Results. Patients with the absence of one posterior connective artery showed a significant stroke severity according to the National Institutes of Health Stroke Scale. The largest subgroup with the absence of both posterior connective arteries was identified, having the best indicators on the Rivermead scale before treatment and a good recovery for neurological deficit after treatment. Significant positive results by the Rivermead scale after treatment were shown in the subgroup with the absence of all connecting arteries. Conclusion. The variant of the Willis circle structure in the form of the absence of both posterior connective arteries in patients with acute cerebrovascular accident is most often found with a good recovery of neurological deficit.

scholarly journals Congenital Cytomegalovirus Infection - Lessons from a Clinical Case

Folia Medica ◽  
2020 ◽  
Vol 62 (2) ◽  
pp. 403-407
Author(s):  
Zhivka Stoykova ◽  
Liliya Ivanova ◽  
Snejinka Cvetkova ◽  
Diana Yordanova
Keyword(s):  
Latent Infection ◽  
Clinical Manifestations ◽  
Specific Treatment ◽  
Congenital Infection ◽  
Rt Pcr ◽  
Cmv Infection ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Polymerase Chain ◽  
The Brain

Background: Cytomegalovirus (CMV) is a worldwide spread herpes virus that establishes a latent infection after the primary infection. It becomes a major problem in immunocompromised patients and in cases of primary or reactivated infection during pregnancy. CMV is the most common congenital infection and is the leading infectious cause of sensoneural deafness and cerebral mental retardation. Aim: Тo raise the attention to the discordance in our knowledge of cCMV infection and the implementation of the knowledge in prac­tice. Materials and methods: CMV-DNA was extracted from saliva and plasma samples according to the protocol of DNA-Sorb-A, Sacace, Biotechnologies, Italy. Polymerase chain reaction (PCR) was performed using a commercial kit based on the Taq-man principle: Quantitative RT-PCR for CMV-DNA detection (Sacace, Biotechnologies, Italy). The linear range of the CMV Real-TM Quant PCR test is 500-10000000 copies/ml, and the reported sensitivity is 400 copies/ml. Results: The infant was born with clinical manifestations involving development of hepatosplenomegaly, chorioretinitis, anemia, mi­crocephaly and simultaneous dilatation of the brain ventricles. CMV infection was confirmed using modern PCR studies. Conclusions: This case highlights the need for specific virological/PCR tests to be performed for all children with the least suspected congenital infection, especially when there is an option of a specific treatment.

scholarly journals Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region

2012 ◽  
Vol 45 (3) ◽  
pp. 297-300 ◽  
Author(s):  
Ana Caroline Dalla Bona ◽  
Adriana Lacerda Twerdochlib ◽  
Mário Antônio Navarro-Silva
Keyword(s):  
Dengue Virus ◽  
Genetic Variants ◽  
The State ◽  
Neighbor Joining ◽  
Rt Pcr ◽  
Junction Region ◽  
Serotype 1 ◽  
Dengue Virus Serotypes ◽  
Polymerase Chain ◽  
Cell Culture Line

INTRODUCTION: The precise identification of the genetic variants of the dengue virus is important to understand its dispersion and virulence patterns and to identify the strains responsible for epidemic outbreaks. This study investigated the genetic variants of the capsid-premembrane junction region fragment in the dengue virus serotypes 1 and 2 (DENV1-2). METHODS: Samples from 11 municipalities in the State of Paraná, Brazil, were provided by the Central Laboratory of Paraná. They were isolated from the cell culture line C6/36 (Aedes albopictus) and were positive for indirect immunofluorescence. Ribonucleic acid (RNA) extracted from these samples was submitted to the reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. RESULTS: RT-PCR revealed that 4 of the samples were co-infected with both serotypes. The isolated DENV-1 sequences were 95-100% similar to the sequences of other serotype 1 strains deposited in GenBank. Similarly, the isolated DENV-2 sequences were 98-100% similar to other serotype 2 sequences in GenBank. According to our neighbor-joining tree, all strains obtained in this study belonged to genotype V of DENV-1. The DENV-2 strains, by contrast, belonged to the American/Asian genotypes. CONCLUSIONS: The monitoring of circulating strains is an important tool to detect the migration of virus subtypes involved in dengue epidemics.

scholarly journals Molecular epidemiology of coronavirus in faeces of Brazilian calves and Peruvian camelid herds

2018 ◽  
Vol 12 (01) ◽  
pp. 037-042
Author(s):  
Camila B Rocha ◽  
Luz Alba A.M.G. Fornells ◽  
Miguel Rojas ◽  
Maíra Libetal ◽  
Alberto Manchego ◽  
...  
Keyword(s):  
The State ◽  
Molecular Characteristics ◽  
Rt Pcr ◽  
High Identity ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Cattle Herds ◽  
Enteric Disorders ◽  
Family Based

Introduction: The enteric disorders represent a serious hazard for bovine and camelid breeding. The aim of this study was to examine the frequency of detection and molecular characteristics of enteric coronavirus (CoV) infections in cattle, alpaca, and llama herds bred in family-based farms in Brazil and Peru. Methodology: Stool samples were collected from calves from Brazil and camelids from Peru for detection and characterization of CoV by reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis. Results: 46.5% (47/101) samples from calves and 26.8% (70/261) from alpaca tested positive for CoV. All strains belong to lineage A1 of the Betacoronavirus genus. Phylogenetic analysis showed high identity between CoV strains detected in calves and alpacas. Conclusions: This study characterised CoV strains from dairy cattle herds in the state of Rio de Janeiro, Brazil, and indicated that this virus is spread among the state herds. The results also indicate widespread circulation of CoV among the alpacas of Cuzco, Peru.

Tactics of a general practitioner in case of suspected acute cerebrovascular accident and measures to prevent it

2021 ◽  
pp. 54-60
Author(s):  
Dmitriy Sergeevich Kovalev
Keyword(s):  
General Practitioner ◽  
Cerebrovascular Accident ◽  
Clinical Symptoms ◽  
Pathological Process ◽  
Elderly Age ◽  
Acute Cerebrovascular Accident ◽  
Medical Issues ◽  
Brain Substance ◽  
Severe Pathology ◽  
The Brain

Acute cerebrovascular accident (stroke) is a fairly common severe pathology, which is one of the main causes of death in most countries. Every year in the world, a stroke occurs in 15 million people, of whom about 5 million die, and the same number become disabled. The disease is based on a sudden sharp failure of the normal blood supply to the brain substance due to a rupture or blockage of a blood vessel. Mortality in the first weeks after a stroke can reach 30-35%. Depending on the pathogenesis of the lesion, stroke can be ischemic and hemorrhagic (ischemic occurs about 6 times more often); clinical symptoms will depend on which area of the brain is involved in the pathological process. Predisposing factors include heredity, gender (more often observed in men), age (in elderly age, the risk of stroke increases every year), arterial hypertension, diabetes mellitus, obesity, hypodynamia. Since in 80% of cases after a stroke people become disabled, often unable to take care of themselves, the problem of acute cerebrovascular accident has long passed from the category of purely medical issues to the category of socio-economic ones. The tasks of the general practitioner include the timely identification of factors predisposing to the development of a stroke and minimization of the possible risks of this formidable condition, and in the case of a stroke, active participation in the patient's rehabilitation process.

scholarly journals A trial of the use of perfusion computed tomography of the brain in combination with transcranial Doppler ultrasonography of blood vessels in patients with acute cerebrovascular accident

2020 ◽  
Vol 101 (1) ◽  
pp. 124-131
Author(s):  
K G Valeeva ◽  
S K Perminova ◽  
A Ya Nazipova ◽  
S V Kurochkin ◽  
A A Yakupova
Keyword(s):  
Computed Tomography ◽  
Blood Flow ◽  
Transcranial Doppler ◽  
Cerebrovascular Accident ◽  
Doppler Ultrasonography ◽  
Transcranial Doppler Ultrasonography ◽  
Perfusion Computed Tomography ◽  
Acute Cerebrovascular Accident ◽  
Vascular Beds ◽  
The Brain

Aim. Assessment of cerebral blood flow in various vascular beds in patients with an acute cerebrovascular accident in the acute period by perfusion computed tomography in combination with transcranial Doppler ultrasonography of cerebral vessels. Methods. Data was analyzed from perfusion computed tomography of the brain and transcranial Doppler ultrasonography in 35 patients with an acute cerebrovascular accident, based at the vascular centre of the City Clinical Hospital No. 7 of Kazan. The study included 18 (51.4%) women and 17 (48.6%) men who had arrived in the first hours after a vascular accident. When analyzing the stroke subtype, atherothrombotic subtype was determined in 27 (77.1%) patients, cardioembolic subtype in 5 (14.3%) patients, and 3 (8.6%) patients had had a transient ischemic attack. Results. Perfusion computed tomography is a method that allowed evaluation of the structure of the brain, and blood supply to the anterior cerebral (in 2.9% of the studied patients), middle cerebral (in 62.9% of the studied patients), posterior cerebral (in 11.4% of the studied patients) and vertebral (in 14.2% of the studied patients) arteries of patients with a stroke. The method revealed a zone of critical perfusion (ischemic penumbra) by quantitatively processing perfusion indicators in the anterior cerebral blood flow system (decrease in rate and increase in average volume of cerebral blood flow and average transit time) and in the posterior cerebral circulation system (decrease in blood flow and prolongation of transit time) in the bed of the right vertebral artery). The method also aided the construction of perfusion maps. Transcranial Doppler ultrasonography of cerebral vessels revealed breaches in the cerebral circulation: a decrease in the linear velocity of blood flow in the right middle cerebral arterial bed and in the posterior circulatory system of blood flow in the brain, and an increase in the pulsatility index in all the studied vascular beds. Conclusion. Perfusion computed tomography of the brain in combination with transcranial Doppler ultrasonography is applicable to patients with stroke in various vascular beds, followed by determination of indications for thrombolytic therapy and thrombectomy.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Cloning of the cDNA Encoding Human Platelet CD36: Comparison to PCR Amplified Fragments of Monocyte, Endothelial and HEL Cells

1993 ◽  
Vol 70 (03) ◽  
pp. 500-505 ◽  
Author(s):  
B Wyler ◽  
L Daviet ◽  
H Bortkiewicz ◽  
J-C Bordet ◽  
J L McGregor
Keyword(s):  
Apparent Molecular Weight ◽  
Platelet Glycoprotein ◽  
Rt Pcr ◽  
Post Translational Modifications ◽  
Hel Cells ◽  
Flanking Region ◽  
Polymerase Chain ◽  
A Minor ◽  
Flanking Regions ◽  
Cdna Fragments

SummaryGlycoprotein CD36, also known as GPIIIb or GPIV, is a major platelet glycoprotein that bears the newly identified Naka alloantigen. The aim of this study was to clone platelet CD36 and investigate other forms of CD36-cDNA present in monocytes, endothelial and HEL cells. RNA from above mentioned cells were reverse transcribed (RT), using specific primers for CD36, and amplified by the polymerase chain reaction (PCR) technique. Sequencing the different amplified platelet derived cDNA fragments, spanning the whole coding and flanking regions, showed the near identity between platelet and CD36-placenta cDNA. Platelet CD36-cDNA cross-hybridized, in Southern blots, with RT-PCR amplified cDNA originating from monocytes, endothelial and HEL cells. However, monocytes showed a RT-PCR amplified cDNA fragment (561 bp) that was present in platelets and placenta but not on endothelial on HEL-cells. Northern blot analysis of platelet RNA hybridized with placenta CD36 indicated the presence of a major (1.95 kb) and a minor (0.95 kb) transcript. The 1.95 kb transcript was the only one observed on Northern blots of monocytes, endothelial and HEL cells. These results indicate that the structure of CD36 expressed in platelets is similar, with the exception of the 3’ flanking region, to that of placenta. Differences in apparent molecular weight between CD36 and CD36-like glycoproteins may be due to post-translational modifications.

Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

1994 ◽  
Vol 72 (05) ◽  
pp. 762-769 ◽  
Author(s):  
Toshiro Takafuta ◽  
Kingo Fujirmura ◽  
Hironori Kawano ◽  
Masaaki Noda ◽  
Tetsuro Fujimoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Immunohistochemical Analysis ◽  
Specific Protein ◽  
Platelet Membrane ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Flow Cytometric ◽  
Polymerase Chain ◽  
Megakaryocytic Cell

SummaryGlycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

Sign in / Sign up

Export Citation Format

Share Document