Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India

2021 ◽  
pp. 1-10
Author(s):  
H. Thukral ◽  
P. Dhaka ◽  
J. Singh Bedi ◽  
R. Singh Aulakh
Keyword(s):  
Risk Factors ◽  
European Union ◽  
Bovine Milk ◽  
Dairy Farms ◽  
Buffalo Milk ◽  
Cow Milk ◽  
Milk Samples ◽  
Significant Difference ◽  
Associated Risk Factors ◽  
Mean Concentration

Aflatoxin M1 (AFM1) contamination in milk and milk products may pose a major public health concern. The present cross-sectional study was aimed to estimate the prevalence of AFM1 in bovine milk across all districts of Punjab, India and to identify the associated animal and farm level risk factors. A total of 402 milk samples (266 cow milk and 136 buffalo milk) were analysed using commercial ELISA and representative samples were confirmed using HPLC-FLD. The results revealed that 56.2 and 13.4% of the milk samples exceeded the maximum levels of the European Union, i.e. 0.05 μg/l and Food Safety and Standards Authority of India (FSSAI), i.e. 0.5 μg/l for AFM1 in milk, respectively. On analysis of species variation, buffalo milk (prevalence: 56.6%; mean concentration: 0.42±0.9 μg/l) was found to have higher AFM1 levels than cow milk (prevalence: 56.0%; mean concentration: 0.19±0.3 μg/l), with statistically significant difference between mean concentrations (P<0.01) and non-significant difference between AFM1 prevalence (P=0.91). Furthermore, milk from commercial dairy farms (prevalence: 64.7%; mean concentration: 0.34±0.65 μg/l) was found to be more contaminated than from household dairy establishments (prevalence: 47.8%; mean concentration: 0.19±0.65 μg/l). The risk factors ‘above average milk yield/day’ (odds ratio (OR): 2.4) and ‘poor animal hygiene’ (OR: 1.9) were identified at animal level, and ‘intensive dairy farming’ (OR: 3.1) and ‘animal feed without aflatoxin binder’ (OR: 4.7) as farm level risk factors for AFM1 excretion above maximum levels of European Union in milk. Among cow breeds, the milk from ‘non-descript’ breed (OR: 11.5) was found to be most contaminated with AFM1 and the least from Jersey breed (OR: 1.0). The present study highlighted the presence of AFM1 in milk samples; therefore, regular monitoring of AFM1 in milk is required so that high risk regions and associated risk factors can be addressed appropriately.

scholarly journals Aflatoxin M1 Contamination in Raw Cow Milk of Dairy Farms in Behbahan area, Khuzestan province, Iran

2019 ◽  
Author(s):  
Seyedeh Ommolbanin Ghasemian
Keyword(s):  
Dairy Farms ◽  
Raw Milk ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Natural Occurrence ◽  
Environmental Chemical ◽  
Khuzestan Province ◽  
Milk Samples ◽  
Significant Difference ◽  
Southwest Of Iran

Introduction: Mycotoxins are among the environmental chemical agents with toxic effects on animals and humans. Aflatoxins (AF) are a major group of mycotoxins produced following the growth of some species of Aspergillus flavus and Aspergillus parasiticus, particularly in agricultural products. Aflatoxin M1 (AFM1) is secreted to milk and remains there since the process of pasteurization and sterilization has little effect on this metabolite. The aim of this study was to investigate the natural occurrence of AFM1 in raw milk in Behbahan area, which is an area of high-density livestock production in southwest of Iran. The other aim was to compare these obtained levels with the accepted level recommended by the European community (50 ng/L). Methods: In the present study, 90 samples of raw milk were collected from five dairy farms in Behbahan region, Khuzestan province, Iran. The AFM1 was analyzed using the Enzyme Linked Immune Sorbent Assay (ELISA) method by ELISA kits from Euro Clone, Italy.  Data were analyzed by ANOVA and the results were reported as mean ± SE. The significant level was set at p ≤ 0.05.  Results: The concentration of aflatoxin M1 in (15)16.54% of raw milk was higher than maximum tolerance limit accepted by European Union /Codex Alimentarius Commission (50 ng/l). The results of this study showed that all 90 investigated raw milk samples were contaminated with AFM1 (100%). Conclusion: A significant difference was observed between concentration means of AFM1 in various studied farms at P < 0.05. Consequently, high occurrence of AFM1 in raw milk samples was considered as a possible hazard for human health. However, further longitudinal studies are required on milk samples and other dairy products in Behbahan as well as other regions of Iran to estimate the representative intake of AFM1 in Iran.

scholarly journals Prevalence of dysmenorrhoea, associated risk factors and its relationship with academic performance among graduating female university students in Ethiopia: a cross-sectional study

BMJ Open ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. e043814
Author(s):  
Mesfin Tadese ◽  
Andargachew Kassa ◽  
Abebaw Abeje Muluneh ◽  
Girma Altaye
Keyword(s):  
Risk Factors ◽  
Academic Performance ◽  
University Students ◽  
Cross Sectional Study ◽  
Secondary Outcome ◽  
Sectional Study ◽  
Cross Sectional ◽  
Grade Point ◽  
Significant Difference ◽  
Associated Risk Factors

ObjectivesThe study aimed to provide an association between dysmenorrhoea and academic performance among university students in Ethiopia. Further, the study attempts to determine the prevalence and associated risk factors of dysmenorrhoea.Design and methodInstitution-based cross-sectional study was conducted from 1 April to 28 April 2019. A semistructured and pretested self-administered questionnaire was used to collect data. Binary logistic regression analysis and one-way analysis of variance were performed to model dysmenorrhoea and academic performance, respectively.Setting and participantsEthiopia (2019: n=647 female university students).OutcomesThe primary outcome is dysmenorrhoea, which has been defined as painful menses that prevents normal activity and requires medication. The self-reported cumulative grade point average of students was used as a proxy measure of academic performance, which is the secondary outcome.ResultsThe prevalence of dysmenorrhoea was 317 (51.5%). The educational status of father (adjusted OR (AOR) (95% CI) 2.64 (1.04 to 6.66)), chocolate consumption (AOR (95% CI) 3.39 (95% 1.28 to 8.93)), daily breakfast intake (<5 days/week) (AOR (95% CI) 0.63 (0.42 to 0.95)), irregular menstrual cycle AOR (95% CI) 2.34 (1.55 to 3.54)) and positive family history of dysmenorrhoea AOR (95% CI) 3.29 (2.25 to 4.81)) had statistically significant association with dysmenorrhoea. There was no statistically significant difference in academic performance among students with and without dysmenorrhoea (F (3611)=1.276, p=0.28)).ConclusionsDysmenorrhoea was a common health problem among graduating University students. However, it has no statistically significant impact on academic performance. Reproductive health officers should educate and undermine the negative academic consequences of dysmenorrhoea to reduce the physical and psychological stress that happens to females and their families.

scholarly journals PREVALENCE OF BOVINE FACIOLOSIS IN HALABA MUNICIPAL ABATTOR SOUTHERN ETHIOPIA

2016 ◽  
Vol 4 (11) ◽  
pp. 82-91
Author(s):  
Muna Abdella ◽  
WubitTafese Mhatebu
Keyword(s):  
Risk Factors ◽  
Body Condition ◽  
Cross Sectional Study ◽  
Control Measures ◽  
Southern Ethiopia ◽  
Multivariable Logistic Regression Analysis ◽  
Cross Sectional ◽  
Significant Difference ◽  
Prevention And Control Measures ◽  
Associated Risk Factors

A cross-sectional study was carried out from November 2015 to march 2016 on bovine fasciolosis, to assess the abattoir based prevalence, predominant species of bovine fasciolosis and associated risk factors of the disease in cattle slaughtered in Halaba municipal abattoir. A total of 384 cattle were examined using post mortem examination. Infection rates were (3.64%), 2.34%, 2.08%, and 1.82 % F. gigantic, F. hepatica, mixed and immature respectively with the overall prevalence of 9.88 % (38). F. gigantic was found to be the most prevalent species in cattle of the study area. Multivariable logistic regression analysis showed that the risk of fasciola infection was significantly higher in poor body condition animals than in medium and good body condition animals (p < 0.05). However, in terms of age, sex and breed, no statistically significant difference was found between infected animals (p > 0.05).The present study showed that bovine fasciolosis is one of the important pathogens in cattle in the study area and warrants appropriate disease prevention and control measures and further epidemiological investigations to determine the different agro ecological risk factors on the occurrence of the disease.

scholarly journals Sensory acceptance and physico-chemical composition of mixed minas frescal cheese

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Bruna Samara dos Santos Rekowsky ◽  
Géssica Cordeiro de Araújo ◽  
Nelson de Carvalho Delfino ◽  
Marion Pereira da Costa ◽  
Thadeu Mariniello Silva
Keyword(s):  
Dry Matter ◽  
Bovine Milk ◽  
Buffalo Milk ◽  
Raw Material ◽  
Cow Milk ◽  
Consumer Market ◽  
Total Solids ◽  
Physico Chemical ◽  
Hedonic Scale ◽  
Milk Mixture

Buffalo milk presents higher proteins and lipids concentration than cow milk, which provides a better yield in cheese-making production and products with considerable sensory approval. However, buffalo milk is not regularly available throughout the year due to different handling conditions. Thus, to guarantee the supply of buffalo milk dairy products during the year, the addition of bovine milk is an alternative. Therefore, this study aimed to test the effect of a buffalo and bovine milk mixture for the Minas Frescal cheese elaboration by physico-chemical, sensory analysis and obtaining yield. The raw material was analysed for the parameters of acidity, density, fat, total solids and solid-not-fat. There were 3 Minas Frescal cheeses elaborated from 3 formulations: 100% buffalo milk, 100% bovine milk and the mixture of 50% of each milk. The cheeses were submitted to the Gravimetric and Gerber methods to obtain values of moisture and fat, as well as to obtain, indirectly, fat in dry matter. For bovine and buffalo milk, the parameters evaluated (acidity, density, fat, total solids and solids-not-fat) complied with Brazilian legislation and parameters described in the literature. For the bovine, mixed and buffalo fresh cheeses, values were obtained, respectively, for moisture (74.04, 60.93 and 63.61), fat in dry matter (44.35, 42.23 and 46.03) and cheese yield (27, 20.8 and 24.2), indicating a higher yield for the bovine Minas Frescal cheese and higher fat content for the buffalo cheese. The overall acceptance of the mixed Minas Frescal cheese was significantly superior to the bovine and buffalo cheese. The parameters of colour, appearance, texture, flavour and overall acceptance were above 8 points in the hedonic scale (‘moderately liked’ to ‘extremely liked’), and the aroma attribute scored was above 7 (‘I enjoyed regularly’ to ‘moderately liked’). Therefore, the elaboration of Minas Frescal cheese from the mixture of 2 matrices (buffalo and bovine milk) demonstrated technological viability with the potential to meet the demands of the consumer market.

scholarly journals Psychological distress and its associated risk factors among university students

2020 ◽  
Vol 66 (4) ◽  
pp. 414-418
Author(s):  
Tingting Li ◽  
Xu Zhang ◽  
Mingming Chen ◽  
Rui Wang ◽  
Lianping He ◽  
...  
Keyword(s):  
Risk Factors ◽  
Psychological Distress ◽  
University Students ◽  
High Intensity ◽  
Education Level ◽  
High Intensity Exercise ◽  
Low Intensity ◽  
Significant Difference ◽  
Associated Risk Factors ◽  
Low Intensity Exercise

SUMMARY OBJECTIVE Psychological distress is an important mental health problem among university students. The goal of this study was to determine psychological distress and its associated risk factors among students in the Anhui province. METHODS A cross-sectional study was conducted in a sample of 1304 students. In this study, a self-administered questionnaire consisting of the general demography and General Health Questionnaire (GHQ-12) was completed. Psychological distress was assessed using the GHQ-12-item questionnaire. A dichotomous category split was imposed on the GHQ-12 for the purpose of analysis. A GHQ-12 score of 4 or higher indicated psychological distress. The data were analyzed by SPSS 20.0 system. RESULTS A total of 1304 samples were analyzed in this study. The results indicated that the education level of the father and mother was associated with the students’ psychological distress (P<0.001). A significant association was found between high-intensity exercise and low-intensity exercise and psychological distress. However, no significant difference was identified between gender and psychological distress (P=0.173). CONCLUSION The education level of parents, high-intensity exercise, and low-intensity exercise were associated with psychological distress. Our results suggest that it is indispensable to raise awareness of psychological disorders and its associated risk factors among university students. Further studies are required to develop appropriate interventions for high-risk groups.

Malnutrition Concerning Geriatric Depression and Associated Risk Factors: A Community-based Case-control Study in Rural Elderly of Bangladesh

2020 ◽  
Author(s):  
Md Ziaul Islam ◽  
Tasnim Disu ◽  
Shatmin Farjana ◽  
Mohammad Rahman
Keyword(s):  
Risk Factors ◽  
Case Control Study ◽  
The Elderly ◽  
Case Control ◽  
Rural Elderly ◽  
Geriatric Depression ◽  
Elderly Individuals ◽  
Significant Difference ◽  
Associated Risk Factors ◽  
Control Study

Abstract Background: Malnutrition and depression are highly prevalent in the elderly and can lead to disparaging outcomes. Analytical studies on malnutrition concerning geriatric depression (GD) are very scarce in Bangladesh, although the size of the elderly population is increasing fast in the country. The current study aimed to assess the association between malnutrition and depression and associated risk factors in the rural elderly.Methods: A case-control study was conducted in 600 elderly residents (aged ≥60 years) of three rural communities of Bangladesh from January to October 2019. Three hundred depressed elderly people were enrolled as cases and 300 non-depressed elderly individuals were included as community controls by matching the age and living area of the cases. We used a semi-structured questionnaire based on the Geriatric Depression Scale-15 and the Bangla version of Mini-Nutritional Assessment-Short Form to collect data through face-to-face interviews. Measures included baseline and personal characteristics, malnutrition, GD, and associated risk factors. A binary logistic regression model was fitted to identify variables associated with the risk of GD.Results: The study found no significant difference in gender (male Vs. female) between cases (44.0% Vs. 56.0%) and controls (46.0% Vs. 54.0%). The study revealed that malnutrition was significantly (p<0.01) higher in cases (56.0%) than in controls (18.0%). The malnourished elderly had around three times AOR=3.155; 95% CI: 1.53-6.49, p=0.002) more (risk of having depression than the controls. The unemployed elderly (AOR=4.964; 95% CI: 2.361-10.440; p=0.0001) and the elderly of the lower and middle class (AOR=3.654; 95% CI: 2.266-7.767; p=0.001) were more likely to experience depression. The elderly having a poor diet were more likely to experience depression (AOR=3.384; 95% CI: 1.764-6.703; p=0.0001). The single elderly (AOR=2.368; 95% CI: 1.762-6.524; p=0.001) and the elderly tobacco users (AOR=2.332; 95% CI: 1.663-5.623; p=0.003) were more likely to experience depression.Conclusions: A significant association between malnutrition and depression is evident in the rural elderly individuals of Bangladesh. It will be a prolific initiative if policymakers merge malnutrition and the risk factors associated with geriatric depression in the provision of universal health care for better health and well-being of the rural elderly populations.

scholarly journals Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk

2018 ◽  
Vol 38 (8) ◽  
pp. 1577-1583 ◽  
Author(s):  
José C. Ribeiro Júnior ◽  
Pedro I. Teider Junior ◽  
André L.M. Oliveira ◽  
Edson A. Rios ◽  
Ronaldo Tamanini ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Goat Milk ◽  
Raw Milk ◽  
Species Level ◽  
Cow Milk ◽  
Production Potential ◽  
Gram Negative ◽  
Pseudomonas Spp ◽  
Milk Samples ◽  
The Mean

ABSTRACT: Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat’s and cow’s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.

Seroprevalence of brucellosis and its associated risk factors in cattle from smallholder dairy farms in Zimbabwe

2011 ◽  
Vol 43 (5) ◽  
pp. 975-982 ◽  
Author(s):  
Gift Matope ◽  
Evison Bhebhe ◽  
John Bwalya Muma ◽  
James Oloya ◽  
Rachel L. Madekurozwa ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dairy Farms ◽  
Smallholder Dairy ◽  
Associated Risk Factors ◽  
Smallholder Dairy Farms

Changes in Voice- and Swallowing-Related Symptoms After Thyroidectomy: One-Year Follow-Up Study

2018 ◽  
Vol 127 (3) ◽  
pp. 171-177 ◽  
Author(s):  
Young Min Park ◽  
Kyung Ho Oh ◽  
Jae-Gu Cho ◽  
Seung-Kuk Baek ◽  
Soon-Young Kwon ◽  
...  
Keyword(s):  
Risk Factors ◽  
Symptom Relief ◽  
Temporal Changes ◽  
Rehabilitation Programs ◽  
Significant Difference ◽  
One Year ◽  
Associated Risk Factors ◽  
Changes Over Time ◽  
Over Time

Objective: We analyzed the changes in voice- and swallowing-related symptoms that occurred over time in patients who underwent thyroidectomy and identified any associated risk factors. Methods: One hundred and three patients who underwent thyroidectomy were enrolled. Results: The mean thyroidectomy voice-related questionnaire (TVQ) score before surgery was 12.41 ± 12.19; it significantly increased to 28.24 ± 18.01 ( P < .001) 1 month postoperatively, decreased to 24.02 ± 17.30 ( P = .014) and 20.66 ± 15.29 ( P = .023) 3 and 6 months postoperatively, respectively. It was continuously decreased to 18.83 ± 14.63 twelve months postoperatively. The temporal changes in TVQ scores between patients who underwent total thyroidectomy or lobectomy were significantly different. There was a statistically significant difference in the temporal changes in TVQ according to whether neck dissection was performed. The temporal changes in TVQ in patients with and without extrathyroidal extension were significantly different. Conclusions: Voice- and swallowing-related discomfort in patients who received thyroidectomy showed dynamic changes over time. There was a significant difference in the degree of change according to clinicopathological factors. Patients with these risk factors may benefit from appropriate patient education and various rehabilitation programs for symptom relief.

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