Rich dynamics of a simple delay host-pathogen model of cell-to-cell infection for plant virus

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

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Search mode for rapid detection of virus particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085733 ◽

1991 ◽

Vol 49 ◽

pp. 286-287

Author(s):

O. E. Bradfute

Keyword(s):

Electron Microscopy ◽

Rapid Detection ◽

Plant Virus ◽

Low Dose ◽

Search Mode ◽

Virus Diseases ◽

Virus Particles ◽

Focus Image ◽

Time Required ◽

Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

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COVID 19 – Eye Issues

Medical & Clinical Research ◽

10.33140/mcr.05.005 ◽

2020 ◽

Vol 5 (Special) ◽

Keyword(s):

Host Cell ◽

Target Cell ◽

Cell Receptor ◽

Human Tissues ◽

Type Ii ◽

Cell Infection ◽

Host Cell Receptor ◽

Infected Cells ◽

Cellular Entry

The coronavirus illness (COVID-19) is caused by a new recombinant SARS-CoV (SARS-CoV) virus (SARS-CoV-2). Target cell infection by SARS-CoV is mediated by the prickly protein of the coronavirus and host cell receptor, enzyme 2 converting angiotensin (ACE2) [3]. Similarly, a recent study suggests that cellular entry by SARS-CoV-2 is dependent on both ACE2 as well as type II transmembrane axial protease (TMPRSS2) [4]. This means that detection of ACE2 and PRSS2 expression in human tissues can predict potential infected cells and their respective effects in COVID-19 patients [1].

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