scholarly journals Addition of an N-Terminal Poly-Glutamate Fusion Tag Improves Solubility and Production of Recombinant TAT-Cre Recombinase in Escherichia coli

2020 ◽  
Vol 30 (1) ◽  
pp. 109-117
Author(s):  
A-Hyeon Kim ◽  
Soohyun Lee ◽  
Suwon Jeon ◽  
Goon-Tae Kim ◽  
Eun Jig Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cre Recombinase ◽  
Fusion Tag ◽  
Terminal Poly

Hirudin as a novel fusion tag for efficient production of lunasin in Escherichia coli

2017 ◽  
Vol 47 (6) ◽  
pp. 619-626 ◽  
Author(s):  
Qinghua Tian# ◽  
Ping Zhang# ◽  
Zhan Gao ◽  
Hengli Li ◽  
Zhengli Bai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Efficient Production ◽  
Fusion Tag

scholarly journals Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56363 ◽  
Author(s):  
Min-Feng Hsu ◽  
Tsung-Fu Yu ◽  
Chia-Cheng Chou ◽  
Hsu-Yuan Fu ◽  
Chii-Shen Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Haloarcula Marismortui ◽  
Fusion Tag

scholarly journals Ubiquitin-like prokaryotic MoaD as a fusion tag for expression of heterologous proteins in Escherichia coli

2014 ◽  
Vol 14 (1) ◽  
pp. 5 ◽  
Author(s):  
Sujuan Yuan ◽  
Xin Wang ◽  
Jian Xu ◽  
Zheng Yan ◽  
Nan Wang
Keyword(s):  
Escherichia Coli ◽  
Heterologous Proteins ◽  
Fusion Tag

scholarly journals Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase

2020 ◽  
Author(s):  
Daniel P. Nickerson ◽  
Monique A. Quinn ◽  
Joshua M. Milnes
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Synthetic Biology ◽  
Cre Recombinase ◽  
Shuttle Vectors ◽  
Genetics Research ◽  
Complete Set ◽  
Rapid Conversion

ABSTRACTPlasmid shuttle vectors capable of replication in both Saccharomyces cerevisiae and Escherichia coli and optimized for controlled modification in vitro and in vivo are a key resource supporting yeast as a premier system for genetics research and synthetic biology. We have engineered a series of yeast shuttle vectors optimized for efficient insertion, removal and substitution of plasmid yeast replication loci, allowing generation of a complete set of integrating, low copy and high copy plasmids via predictable operations as an alternative to traditional subcloning. We demonstrate the utility of this system through modification of replication loci via Cre recombinase, both in vitro and in vivo, and restriction endonuclease treatments.

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