Physicochemical, antibacterial properties, and compatibility of ZnO-NPs/chitosan/β-glycerophosphate composite hydrogels

Wound Dressing ◽

Mesoporous Silica Nanoparticles ◽

Healing Process ◽

Release Time ◽

Wound Healing Process ◽

Zno Nps ◽

And Control ◽

Scanning Electron

Hydrogels are biocompatible and swollen materials that have been used as a wound dressing for years. Among them, chitosan-based hydrogels have become popular in the wound healing process owing to their low toxic, biocompatible, biodegradable, antibacterial properties. Chitosan (CS) has been used either as a pure form or incorporated with polymers or nanoparticles to increase antimicrobial activity and stability. In this context, zinc oxide nanoparticles (ZnO NPs) have been used to enhance antibacterial activity and mesoporous silica nanoparticles (MSN) have been employed to develop mechanical strength and control of drug release time. In this study, we report the synthesis and fully characterizations of ZnO NPs, MSN and the hydrogel by using dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). We have also prepared and characterized chitosan-based hydrogels functionalized by MSNs and ZnO NPs.

Structure and Properties of Biodegradable PLLA/ZnO Composite Membrane Produced via Electrospinning

Materials ◽

10.3390/ma14010002 ◽

2020 ◽

Vol 14 (1) ◽

pp. 2

Author(s):

Daria A. Goncharova ◽

Evgeny N. Bolbasov ◽

Anna L. Nemoykina ◽

Ali A. Aljulaih ◽

Tamara S. Tverdokhlebova ◽

...

Keyword(s):

Analytical Techniques ◽

Fiber Material ◽

Composite Membranes ◽

Inorganic Nanoparticles ◽

Zno Nps ◽

E Coli ◽

Standard Procedures ◽

Modified Surface ◽

First Time

These days, composite materials based on polymers and inorganic nanoparticles (NPs) are widely used in optoelectronics and biomedicine. In this work, composite membranes of polylactic acid and ZnO NPs containing 5–40 wt.% of the latter NPs were produced by means of electrospinning. For the first time, polymer material loaded with up to 40 wt.% of ZnO NPs (produced via laser ablation in air and having non-modified surface) was used to prepare fiber-based composite membranes. The morphology, phase composition, mechanical, spectral and antibacterial properties of the membranes were tested by a set of analytical techniques including SEM, XRD, FTIR, UV-vis, and photoluminescence spectroscopy. Antibacterial activity of the materials was evaluated following standard procedures (ISO 20743:2013) and using S. aureus and E. coli bacteria. It is shown that incorporation of 5–10 wt.% of NPs led to improved mechanical properties of the composite membranes, while further increase of ZnO content up to 20 wt.% and above resulted in their noticeable deterioration. At the same time, the antibacterial properties of ZnO-rich membranes were more pronounced, which is explained by a larger number of surface-exposed ZnO NPs, in addition to those embedded into the bulk of fiber material.

Biocompatibility and Antibacterial Properties of ZnO-Incorporated Anodic Oxide Coatings on TiZrNb Alloy

Nanomaterials ◽

10.3390/nano10122401 ◽

2020 ◽

Vol 10 (12) ◽

pp. 2401

Author(s):

Oleksandr Oleshko ◽

Yevheniia Husak ◽

Viktoriia Korniienko ◽

Roman Pshenychnyi ◽

Yuliia Varava ◽

...

Keyword(s):

Contact Angle ◽

Zno Nanoparticles ◽

Cell Attachment ◽

Oxide Coating ◽

Anodic Oxide ◽

Ceramic Coatings ◽

Zno Nps ◽

Low Modulus ◽

Ca Ions

In a present paper, we demonstrate novel approach to form ceramic coatings with incorporated ZnO nanoparticles (NPs) on low modulus TiZrNb alloy with enhanced biocompatibility and antibacterial parameters. Plasma Electrolytic Oxidation (PEO) was used to integrate ZnO nanoparticles (average size 12–27 nm), mixed with Ca(H2PO2)2 aqueous solution into low modulus TiZrNb alloy surface. The TiZrNb alloys with integrated ZnO NPs successfully showed higher surface porosity and contact angle. XPS investigations showed presence of Ca ions and absence of phosphate ions in the PEO modified layer, what explains higher values of contact angle. Cell culture experiment (U2OS type) confirmed that the surface of as formed oxide-ZnO NPs demonstrated hydrophobic properties, what can affect primary cell attachment. Further investigations showed that Ca ions in the PEO coating stimulated proliferative activity of attached cells, resulting in competitive adhesion between cells and bacteria in clinical situation. Thus, high contact angle and integrated ZnO NPs prevent bacterial adhesion and considerably enhance the antibacterial property of TiZrNb alloys. A new anodic oxide coating with ZnO NPs could be successfully used for modification of low modulus alloys to decrease post-implantation complications.

Does the Addition of Zinc Oxide Nanoparticles Improve the Antibacterial Properties of Direct Dental Composite Resins? A Systematic Review

Materials ◽

10.3390/ma14010040 ◽

2020 ◽

Vol 14 (1) ◽

pp. 40

Author(s):

Divya Arun ◽

Dulanja Adikari Mudiyanselage ◽

Rumana Gulam Mohamed ◽

Michael Liddell ◽

Nur Mohammad Monsur Hassan ◽

...

Keyword(s):

Systematic Review ◽

Zinc Oxide ◽

Composite Resins ◽

Dental Composite ◽

Cochrane Library ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Dental Composite Resins

A promising approach to improve the poor antibacterial properties of dental composite resins has been the addition of metal oxide nanoparticles into the resin matrix. This systematic review aimed to determine whether the addition of zinc oxide nanoparticles (ZnO-NPs) improves the antibacterial properties of direct dental composite resins. This review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and registered with the PROSPERO database: CRD42019131383. A systematic literature search was conducted using the following databases: Medline (Ovid), the Cochrane Library, SCOPUS, CINAHL, Web of Science, Trove, Google Scholar, World Cat, and OpenGrey. The initial search retrieved 3178 results, which were then screened against inclusion and exclusion criteria, resulting in a total of four studies that were eligible for qualitative synthesis within this review. All the included studies were in vitro non-randomized post-test design experimental studies. A lack of congruity in the results obtained from these studies that used different tests to evaluate antibacterial activity was evident. Although some studies demonstrated a significant improvement of antibacterial properties in composites containing at least 1% ZnO-NPs (wt %), they are unlikely to present any clear clinical advantage due to the short lifetime of observed antibacterial properties.

Mechanistic Study of Antibacterial Properties of Chemically Synthesize Zinc Oxide Nanoparticles

Advanced Nano Research ◽

10.21467/anr.2.1.42-52 ◽

2019 ◽

Vol 2 (1) ◽

pp. 42-52

Author(s):

Abdur Rehman ◽

Saira Ahmad ◽

Abdul Mateen ◽

Huma Qamar ◽

Mudaber Ahmad Mubashar ◽

...

Keyword(s):

Zinc Oxide ◽

Zno Nanoparticles ◽

Bacterial Strain ◽

Sol Gel ◽

Mechanistic Study ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Wide Range

Nanotechnology is the science, engineering and technology conducted at the scale that ranges between 1-100 nanometers. For the bio-application, evolution of nanotechnology is creating the concern of scientists towards the synthesis of nanoparticles. The nanoparticles have unique characteristics as compare to bulk materials. Zinc oxide (ZnO) is a matchless semiconductor and it has been under investigation due to its wide range of applications in various areas like biomedical, electronics, material science and optics. In the present work synthesis of ZnO nanoparticles was carried out by using simple chemical approach, Sol-gel method for being effective and inexpensive, by employing zinc acetate dehydrate Zn (CH3CO2)2.2H2O as a precursor and sodium hydroxide (NaOH) starch as a constant agent. The structural properties of resultant zinc oxide nanoparticles were investigated by X-ray diffraction (XRD) technique. The XRD data confirmed the hexagonal wurtzite structure of ZnO powder confirmed by JCPDS 36-1451 data. Particles size was calculated by Scherrer formula and calculated size was 30.14 nm. These nanoparticles were investigated for inhibition zone of bacterial strain Escherichia coli, a gram-negative microbe, at various concentrations of ZnO nanoparticles. Zinc oxide nanoparticles were very proficient for inhibition of growth of bacterial strain E. coli. The mechanism of ZnO NPs for antibacterial activity is release of reactive oxygen species which not only hydrolyze cell wall but cell membrane and cellular components as well providing a potential bactericidal effect.

New Organosilicon Composite Based on Borosiloxane and Zinc Oxide Nanoparticles Inhibits Bacterial Growth, but Does Not Have a Toxic Effect on the Development of Animal Eukaryotic Cells

Materials ◽

10.3390/ma14216281 ◽

2021 ◽

Vol 14 (21) ◽

pp. 6281

Author(s):

Denis N. Chausov ◽

Dmitriy E. Burmistrov ◽

Alexander D. Kurilov ◽

Nikolay F. Bunkin ◽

Maxim E. Astashev ◽

...

Keyword(s):

Zinc Oxide ◽

Composite Material ◽

Comprehensive Analysis ◽

Oxide Nanoparticles ◽

Oxygen Species ◽

Zno Nps ◽

E Coli ◽

Positive Effect

The present study a comprehensive analysis of the antibacterial properties of a composite material based on borosiloxane and zinc oxide nanoparticles (ZnO NPs). The effect of the polymer matrix and ZnO NPs on the generation of reactive oxygen species, hydroxyl radicals, and long-lived oxidized forms of biomolecules has been studied. All variants of the composites significantly inhibited the division of E. coli bacteria and caused them to detach from the substrate. It was revealed that the surfaces of a composite material based on borosiloxane and ZnO NPs do not inhibit the growth and division of mammalians cells. It is shown in the work that the positive effect of the incorporation of ZnO NPs into borosiloxane can reach 100% or more, provided that the viscoelastic properties of borosiloxane with nanoparticles are retained.

Green Fabrication of Zinc Oxide Nanoparticles Using Phlomis Leaf Extract: Characterization and In Vitro Evaluation of Cytotoxicity and Antibacterial Properties

Molecules ◽

10.3390/molecules26206140 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6140

Author(s):

Amal A. Alyamani ◽

Salim Albukhaty ◽

Salman Aloufi ◽

Faizah A. AlMalki ◽

Hassan Al-Karagoly ◽

...

Keyword(s):

Zinc Oxide ◽

Leaf Extract ◽

Normal Fibroblast ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Disk Diffusion Method

Green nanoparticle synthesis is an environmentally friendly approach that uses natural solvents. It is preferred over chemical and physical techniques due to the time and energy savings. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) through a green method that used Phlomis leaf extract as an effective reducing agent. The synthesis and characterization of ZnO NPs were confirmed by UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Dynamic light scattering (DLS), Zeta potential, and Field Emission Scanning Electron Microscope (FESEM) techniques. In vitro cytotoxicity was determined in L929 normal fibroblast cells using MTT assay. The antibacterial activity of ZnO nanoparticles was investigated using a disk-diffusion method against S. aureus and E. coli, as well as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) content concentrations. XRD results confirmed the nanoparticles’ crystalline structure. Nanoparticle sizes were found to be around 79 nm by FESEM, whereas the hydrodynamic radius of nanoparticles was estimated to be around 165 3 nm by DLS. FTIR spectra revealed the formation of ZnO bonding and surfactant molecule adsorption on the surface of ZnO NPs. It is interesting to observe that aqueous extracts of phlomis leave plant are efficient reducing agents for green synthesis of ZnO NPs in vitro, with no cytotoxic effect on L929 normal cells and a significant impact on the bacteria tested.

A review of the green synthesis of ZnO nanoparticles using plant extracts and their prospects for application in antibacterial textiles

Journal of Engineered Fibers and Fabrics ◽

10.1177/15589250211046242 ◽

2021 ◽

Vol 16 ◽

pp. 155892502110462

Author(s):

Jun Xu ◽

Yubo Huang ◽

Shihui Zhu ◽

Nedra Abbes ◽

Xiaoning Jing ◽

...

Keyword(s):

Green Synthesis ◽

Plant Extracts ◽

Environmental Sustainability ◽

Future Trend ◽

Precursor Concentration ◽

Morphological Properties ◽

Zno Nps ◽

Research Attention ◽

Antibacterial Textiles

In an era of environmentally friendly development, methods of the green synthesis of zinc oxide nanoparticles (ZnO NPs) from plant extracts have become a focus of research attention because of the benefits of environmental sustainability, simplicity, and low price. The present review introduces a green mechanism for the synthesis of ZnO NPs using the extracts of plants, exploring factors that influence the morphology of ZnO NPs and their antibacterial properties, and the mechanisms of antibacterial action. The results indicate that the factors that influence morphology include the intrinsic crystallographic morphological properties and conditions of the preparation of ZnO NPs. In terms of preparation conditions, the influence of plant extract concentration, precursor concentration, reaction time, and calcination temperature on NP morphology is related to the species of plants used, with precursor concentration the most significant factor affecting the morphology of ZnO NPs. A pH of 12 appears be the most appropriate alkalinity for the synthesis of ZnO NPs from plant extracts. In addition, the synthesized ZnO NPs display excellent antibacterial properties, the mechanism of which involves photocatalysis, reactive oxygen species, and interactions between ZnO NPs and bacterial surfaces. Factors influencing the antibacterial properties are the type of bacteria and the concentration and morphology of ZnO NPs. Finally, the methods of preparation of antibacterial textiles using synthetic ZnO NPs are discussed in relation to the preparation of antibacterial fibers, fabric, and composite textiles. Here, the future trend of such antibacterial textiles is considered, providing the direction for further research of antibacterial textiles.

Facile Green Synthesis of LaCe Co-Doped ZnO Nanoparticles and Their Structural, Optical and Antibacterial Properties

Journal of Nanoscience and Technology ◽

10.30799/jnst.184.18040524 ◽

2018 ◽

Vol 4 (5) ◽

pp. 552-554

Author(s):

M. Karthikeyan ◽

A. Jafar Ahamed ◽

P. Vijaya Kumar

Keyword(s):

Green Synthesis ◽

Zno Nanoparticles ◽

Synthesis Method ◽

Inert Gases ◽

Diffusion Method ◽

Zno Nps ◽

Vis Spectroscopy ◽

Doped Zno ◽

Co Doped

This present work describes the synthesis of LaCe co-doped zinc oxide (ZnO) nanoparticles (NPs) prepared by green method using Gymnema sylvestre (G. sylvestre) leaves as reducing as well as capping agent. Green synthesis method avoids inert gases, high pressure, laser radiation, high temperature, toxic chemicals etc. as compared to conventional method like sol-gel technique method, laser ablation method, solvothermal method, inert gas condensation method and chemical reduction method. The synthesized LaCe co-doped ZnO NPs was characterized by X-Ray diffraction (XRD), field emission scanning electron microscopy (FESEM), elemental analysis (EADX), Fourier transform infrared spectroscopy (FTIR), UV-vis spectroscopy and photoluminescence (PL). The LaCe co-doped ZnO NPs was tested against clinical pathogens such as gram positive G+ (Staphylococcus aureus and Streptococcus pneumoniae) and gram negative G- (Klebsiella pneumoniae, Shigella sydenteriae, Escherichia coli, Pseudomonas aeruginosa and Protus vulgaris) bacterial strains using agar well diffusion method.

Investigation of Functionalized Surface Charges of Thermoplastic Starch/Zinc Oxide Nanocomposite Films Using Polyaniline: The Potential of Improved Antibacterial Properties

Polymers ◽

10.3390/polym13030425 ◽

2021 ◽

Vol 13 (3) ◽

pp. 425

Author(s):

Korakot Charoensri ◽

Chatchai Rodwihok ◽

Duangmanee Wongratanaphisan ◽

Jung A. Ko ◽

Jin Suk Chung ◽

...

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Thermoplastic Starch ◽

Nanocomposite Films ◽

Resistant Bacteria ◽

Surface Charges ◽

Zno Nps ◽

E Coli ◽

Bionanocomposite Films

Improving the antibacterial activity of biodegradable materials is crucial for combatting widespread drug-resistant bacteria and plastic pollutants. In this work, we studied polyaniline (PANI)-functionalized zinc oxide nanoparticles (ZnO NPs) to improve surface charges. A PANI-functionalized ZnO NP surface was prepared using a simple impregnation technique. The PANI functionalization of ZnO successfully increased the positive surface charge of the ZnO NPs. In addition, PANI-functionalized ZnO improved mechanical properties and thermal stability. Besides those properties, the water permeability of the bionanocomposite films was decreased due to their increased hydrophobicity. PANI-functionalized ZnO NPs were applied to thermoplastic starch (TPS) films for physical properties and antibacterial studies using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The PANI-functionalized ZnO bionanocomposite films exhibited excellent antibacterial activity for both E. coli (76%) and S. aureus (72%). This result suggests that PANI-functionalized ZnO NPs can improve the antibacterial activity of TPS-based bionanocomposite films.