Comparative Researches on Two Kinds of Staining Method of Acetylcholinesterase Isozymes

2013 ◽  
Vol 641-642 ◽  
pp. 3-6 ◽  
Author(s):  
Gan Zhao ◽  
Kang Jie Chen ◽  
Shao Cong Wu ◽  
Qin Chuan Zhuang ◽  
Fang Qian
Keyword(s):  
Staining Method ◽  
The Other ◽  
Acetylthiocholine Iodide ◽  
Staining Methods

The staining effects of two staining methods of acetylcholinesterase isozymes were compared . One using acetylthiocholine iodide , K3[Fe(CN)6] and CuSO4 was named as AFCu method, and the other one using acetylthiocholine iodide , K3[Fe(CN)6] and FeCl3 was named as AFFe method. The staining effect of former was better.

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

scholarly journals Immunocytochemical localization of the posterior lobe hormones and of their carrier proteins.

1980 ◽  
Vol 28 (5) ◽  
pp. 469-471 ◽  
Author(s):  
F Vandesande ◽  
K Dierickx ◽  
N Goossens
Keyword(s):  
Staining Method ◽  
Posterior Lobe ◽  
Serial Sections ◽  
Double Staining ◽  
Carrier Proteins ◽  
Tissue Sections ◽  
Tissue Antigens ◽  
Staining Methods ◽  
Double Staining Method ◽  
The One

Serial sections of vertebrate hypothalami were stained with the immunocytochemical peroxidase-antiperoxidase method. In addition to the single staining method, our double staining method was used, which enabled us to visualize two tissue antigens in single tissue sections. In both staining methods, differentially adsorbed antineurohypophysial hormone sera, anti-somatostatin serum and anti-bovine neurophysin sera were used. The results confirm the one hormone, one neuron hypothesis.

Black mulberries (Morus nigra) as a natural dye for animal tissues staining

2011 ◽  
Vol 61 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Ehab Tousson ◽  
Bahija Al-Behbehani
Keyword(s):  
Staining Method ◽  
Physiological Saline ◽  
Natural Dyes ◽  
New Method ◽  
Beet Root ◽  
Morus Nigra ◽  
Whole Mount ◽  
Physiological Saline Solution ◽  
Staining Methods ◽  
Liver Flukes

AbstractNatural dyes produce an extraordinary diversity of rich and complex colours as well as unexpected results, making them exciting to use. Natural dyes have been used for staining wool, silk, carpet and cotton. Black mulberry (Morus nigra) has strong staining activity and a distinct flavor with juicy and acidic characteristics making them attractive for use in the processing industry in products such as fruit juice, ice cream, jelly, and jam. Aim of this study was to investigate a new staining method using black mulberry for whole mount and transverse sections staining of fasciola. Adult liver flukes (Fasciola sp.) were collected from the livers of naturally infected cows at slaughterhouse, washed with physiological saline solution. Some adult Fasciola were collected, immersed in 10% neutral buffered formalin for fixation, and embedded in paraffin for histological studies. The rest of whole mount of adult worms were collected, and then stained by the new method (dye extracted form beet root) and Carmine staining method for control. Sections, 7-10 micrometer from adult worms were collected, and then stained by the new method and hematoxyllin & eosin staining method for control. By using the dye extracted from beet root, zoologists and parasitologists can make identification and differentiation between different parasites. By using the dye extracted from black mulberry, zoologists and parasitologists can make identification and differentiation between different parasites. This dye method can be an alternative to cost and time consuming current chemical staining methods.

Nucleolar organizer activity at meiosis in wheat–rye hybrid plants

1986 ◽  
Vol 28 (2) ◽  
pp. 227-234 ◽  
Author(s):  
N. Cuñado ◽  
M. C. Cermeño ◽  
J. Orellana
Keyword(s):  
Silver Staining ◽  
Staining Method ◽  
Nucleolar Organizer ◽  
The Other ◽  
Nucleolar Organizer Regions ◽  
Binucleate Cells ◽  
Hybrid Plants ◽  
Nucleolar Organizer Activity ◽  
Silver Staining Method ◽  
Organizer Activity

Nucleoli and nucleolar organizer regions (NORs) have been studied by a silver staining method in all meiotic stages of wheat–rye hybrid plants. The maximum number of nucleoli per cell scored at meiotic prophase and tapetum binucleate cells indicates that only the NORs of 1B, 6B, and 5D wheat chromosomes are active, whereas that of chromosome IR (SAT) of rye is inactive. However, at diakinesis, metaphase and anaphase meiotic stages only chromosomes 1B and 6B show Ag-NOR as was reported previously in somatic metaphase. The absence of Ag-NOR on chromosome 5D has been imputed to its low nucleolar organizer activity, not detectable by silver staining, because of the small number of rDNA clusters it carries. On the other hand, the meiotic behaviour of chromosomes 1B and 6B has been studied at metaphase I and anaphase I, using the Ag-NORs as cytological markers.Key words: nucleolar organizer, Ag-NOR, meiosis, wheat–rye hybrids.

Fine Structure of the Elastic Fibers and the Elastin Crystalloids by Means of Tannic Acid Fixation Method

1976 ◽  
Vol 34 ◽  
pp. 318-319
Author(s):  
V. Mizuhira ◽  
Y. Futaesaku ◽  
H. Nakamura
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Tannic Acid ◽  
Staining Method ◽  
Fixation Method ◽  
Elastic Fibers ◽  
Osmium Tetraoxide ◽  
Ear Cartilage ◽  
Staining Methods

It is well known that the elastic fibers do not stain well with conventional electron fixation and staining methods. However some investigators have tried to demonstrate elastic fibers as a stained structure under the electron microscope. Greenlee et al.(1966) and Ouintarelli et al.(1973) reported a phosphotaungstic acid staining method for elastic fibers; Albert (1970 & 1971) reported his results using a metalic tetraphenylprophine staining method.In the contrast to these staining methods for elastic fibers, Mizuhira et al (1971,'72, ‘75) have succeeded in demonstrating elastic fibers very clearly using an improved fixation method for proteins with glutaraldehyde containing tannic acid, followed by the osmium tetraoxide method.This new fixation method is very simple. Aorta, ear cartilage, intestine or skin are fixed with 2.5 % glutaraldehyde, containing 0.5 % to 4 % tannic acid buffered with veronal acetate or phosphate at pH 6.8 to 7.2 for 1.5 hrs to overnight.

scholarly journals A novel RGB-trichrome staining method for routine histological analysis of musculoskeletal tissues

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Francisco Gaytan ◽  
Concepción Morales ◽  
Carlos Reymundo ◽  
Manuel Tena-Sempere
Keyword(s):  
Musculoskeletal System ◽  
Bone Repair ◽  
Staining Method ◽  
Three Dimensional ◽  
Bone Diseases ◽  
Metabolic Bone Diseases ◽  
Skeletal Tissue ◽  
Musculoskeletal Tissues ◽  
Clear Identification ◽  
Staining Methods

Abstract Morphometry and histology are essential approaches for investigation and diagnosis of musculo-skeletal disorders. Despite the advent of revolutionary methods of image analysis and high resolution three-dimensional imaging technology, basic conventional light microscopy still provides an incisive overview of the structure and tissue dynamics of the musculoskeletal system. This is crucial to both preclinical and clinical research, since several clinically relevant processes, such as bone repair, osteoarthritis, and metabolic bone diseases, display distinct, if not pathognomonic, histological features. Due to the particular characteristics of the skeletal tissues (i.e., the existence of mineralized extracellular matrices), a large number of staining methods applicable to either decalcified or undecalcified tissues are available. However, it is usually the case that several staining methods need to be sequentially applied in order to achieve the different endpoints required to fully assess skeletal tissue structure and dynamics, and to allow morphometric quantification. We describe herein a novel staining method, the RGB trichrome, amenable for application to decalcified, paraffin embedded human musculoskeletal tissues. The acronym RGB corresponds to the three primary dyes used: picrosirius Red, fast Green, and alcian Blue. Although these individual pigments are commonly used either isolated, in binary combinations, or as part of more complex polychrome staining methods, when merged in the RGB trichrome staining produce high-quality/high-contrast images, permitting not only clear identification of different tissues (i.e., the different types of cartilage, bone and fibrous connective tissue), but also discrimination between calcified and uncalcified bone and cartilage, as well as an unexpected diversity of shades of color, while displaying singular properties among polychrome staining methods, such as the unveiling of the bone osteocyte dendritic/canalicular network. Hence, we propose the RGB trichrome as simple but highly-reliable tool for the preclinical and clinical study of the musculoskeletal system.

The detection of imidazoles including histidine and some of its derivatives in biological fluids

1969 ◽  
Vol 47 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Arthur E. Pasieka ◽  
M. E. Thomas
Keyword(s):  
Amino Acids ◽  
High Voltage ◽  
Mercuric Chloride ◽  
Staining Method ◽  
Tap Water ◽  
Biological Fluids ◽  
The Other ◽  
Synthetic Mixture ◽  
Bromophenol Blue ◽  
Staining Procedure

A staining method for the detection of imidazoles including histidine and histidine-containing compounds is described. It is based on the treatment of developed high-voltage paper electrograms or paper chromatograms with bromophenol blue. The electrogram or chromatogram after drying is fixed in n-butanol, complexed with mercuric chloride, and subsequently stained with 0.2% bromophenol blue in 95% ethanol. The electrogram or chromatogram is washed while still damp with tap water until the background is clear. None of the other 20 known amino acids including tyrosine, such as are found in the synthetic mixture M150, reacted in the above staining procedure. Purines also react with the described reagent due to their imidazole configuration.

scholarly journals The histochemical distribution of protein bound sulfhydryl groups in human epidermis by the new staining method.

1979 ◽  
Vol 27 (5) ◽  
pp. 942-946 ◽  
Author(s):  
H Ogawa ◽  
A Taneda ◽  
Y Kanaoka ◽  
T Sekine
Keyword(s):  
Cell Membrane ◽  
Stratum Corneum ◽  
Staining Method ◽  
Sulfhydryl Groups ◽  
The Other ◽  
Human Epidermis ◽  
Horny Layer ◽  
Intercellular Spaces ◽  
Sh Groups ◽  
Thiol Reagent

Recently, we synthesized a new fluorescent thiol reagent, N-(7-dimethylamino-4-methylcoumarinyl)-maleimide (DACM) which is nonfluorescent by itself but will react readily with -SH groups to form highly fluorescent addition products. By the use of this reagent, we studied the localization and concentration of -SH groups and S--S linkages in the human epidermis. The distribution of -SH groups in living layers was abundant in cytoplasm but not in nuclei. The fluorescence was concentrated on the cell membrane or intercellular spaces (MIC parts) and was increased at the spino-granular junction. In the horny layer, the fluorescence of the MIC parts appeared brilliantly in the lower layers and decreased gradually. On the other hand, the fluorescence of cytoplasm in keratinized cells in the stratum corneum was faint. The localization of S--S linkages was not a characteristic of the living layers, but appeared abruptly at the junction of living and horny layers. The fluorescence was localized to the MIC parts and disappeared gradually. The distribution of S--S linkages appeared to be very low in the cytoplasm of keratinized cells. No substantial fluorescence was localized on keratohyalin granules even after reduction.

scholarly journals Radioimmunocytochemistry-a novel immunocytochemical principle.

1977 ◽  
Vol 25 (10) ◽  
pp. 1140-1146 ◽  
Author(s):  
L I Larsson ◽  
T W Schwartz
Keyword(s):  
The Other ◽  
Tissue Antigen ◽  
Quantitative Immunocytochemistry ◽  
Immunocytochemical Techniques ◽  
Staining Methods

Currently available immunocytochemical techniques rely on the detection of the antibody by means of direct labeling or indirect immunologic procedures. To increase immunocyto-chemical specificity a new radioimmunocytochemical method has been developed. In the radioimmunocytochemical method radiolabeled antigen is incubated with surplus amount of antibody. In this way only one combining site of the antibody will bind the labeled antigen, leaving the other site free to react with tissue-bound antigen. The site of reaction with tissue antigen is revealed by autoradiography. The radioimmunocytochemical method is specific and sensitive and can be combined with conventional staining methods or with immunoperoxidase techniques. It may also be useful for ultrastructural and quantitative immunocytochemistry.

scholarly journals Assessment of stallion semen morphology using two different staining methods, microscopic techniques, and sample sizes

2016 ◽  
Vol 60 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Katarzyna Łącka ◽  
Stanisław Kondracki ◽  
Maria Iwanina ◽  
Anna Wysokińska
Keyword(s):  
Sperm Morphology ◽  
Staining Method ◽  
Microscopic Analysis ◽  
Sample Sizes ◽  
Morphology Analysis ◽  
Morphological Defects ◽  
Staining Methods ◽  
Microscopic Techniques ◽  
Semen Morphology

Abstract Introduction: The aim of this study was to propose the optimal methodology for stallion semen morphology analysis while taking into consideration the staining method, the microscopic techniques, and the workload generated by a number of samples. Material and Methods: Ejaculates from eight pure-bred Arabian horses were tested microscopically for the incidence of morphological defects in the spermatozoa. Two different staining methods (eosin-nigrosin and eosin-gentian dye), two different techniques of microscopic analysis (1000× and 400× magnifications), and two sample sizes (200 and 500 spermatozoa) were used. Results: Well-formed spermatozoa and those with major and minor defects according to Blom’s classification were identified. The applied staining methods gave similar results and could be used in stallion sperm morphology analysis. However, the eosin-nigrosin method was more recommendable, because it allowed to limit the number of visible artefacts without hindering the identification of protoplasm drops and enables the differentiation of living and dead spermatozoa. Conclusion: The applied microscopic techniques proved to be equally efficacious. Therefore, it is practically possible to opt for the simpler and faster 400x technique of analysing sperm morphology to examine stallion semen. We also found that the number of spermatozoa clearly affects the results of sperm morphology evaluation. Reducing the number of spermatozoa from 500 to 200 causes a decrease in the percentage of spermatozoa identified as normal and an increase in the percentage of spermatozoa determined as morphologically defective.

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