Biocompatibility of Alginate -Graphene Oxide Film for Tissue Engineering Applications

2021 ◽  
Vol 900 ◽  
pp. 26-33
Author(s):  
Ishraq Abd Ulrazzaq Kadhim
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Sodium Alginate ◽  
Cell Lines ◽  
In Vitro Test ◽  
Solvent Casting ◽  
Ftir Analysis ◽  
Casting Method ◽  
Vitro Test

The present paper indicates promising potential of Sodium Alginate) Alg)/Graphene oxide (Go) films in fields bone tissue engineering (TE). The Sodium Alginate (Alg)/Graphene oxide (Go) films, were fabricated via (solvent casting method). The interaction of Sodium Alginate (Alg) with Graphene oxide (Go) via hydrogen bonding was confirmed by FTIR analysis. The swelling degree of Sodium Alginate (Alg)/Graphene oxid (Go) films was also studied. Furthermore, the biocompatibility of Sodium Alginate (Alg)/Graphene oxide (Go) films disclosed its non-cytotoxic effect on the cell lines (MG-63) in-vitro test, the viability of cell lines on the films, and hence its appropriateness as potent biomaterial for tissue engineering.

scholarly journals Studies of Melody Efficacy Vaccine Against SARS-CoV-2 are Closing COVID-19 Pandemic Doors? Single Dose is Recommended as Therapeutic Drug and Two Doses Application as Preventive Vaccine

2021 ◽  
Keyword(s):  
Single Dose ◽  
Cell Lines ◽  
Cell Cultures ◽  
Statistical Difference ◽  
In Vitro Studies ◽  
Therapeutic Drug ◽  
In Vitro Test ◽  
Preventive Vaccine ◽  
Vitro Test

In vitro studies provide data that is important for proof-of-concept determination, function validation, and peer review manuscript preparation, FDA applications and clinical trials. Our in vitro studies of Melody efficacy as a vaccine against SARS-CoV-2 were made in five cell cultures (BEAS-2B, HUVEC, HMEC-1, HEK293 and WM266), where the dead cell number monitored by using flow cytometry. Melody’s efficacy was tested by incubating melody RpA (RNA-peptide A) and melody RpAB (RNA-peptide AB). Both incubations were pending 16 hours with melody Lethal Concentration 50 % (LC50 µg/µL), which was determinated in a toxicological assay in the five cell cultures as previously described. Our conclusion was focused to understand the statistical difference in LC50 in order to estimate the melody preventive efficacity as vaccine in “In vitro” test. In the present study, the preventive efficacy values obtained in the five cell lines differ statistically between the value of two doses when compared with a single or three doses, p=5.321. These results suggested that the application of two doses is more effective. We used the Student’s t-test with n=6 to calculate p-value according to mRNA avian coronavirus action after one, two and three doses incubations of mRNA-peptide-A (melody therapeutic) of 16 hours. Our conclusion was focused to understand the statistical difference in LC50 in order to estimate the melody therapeutic efficacity as vaccine in “In vitro” test in cell lines (BEAS-2B, HUVEC, HMEC-1, HEK293 and WM-266). Results: Melody Therapeutic Efficacity was found to be 93.82% with using a single dose. Meanwhile, Melody Preventive Efficacity was found as 92.53% with using two doses. The therapeutic efficacy values obtained in the five cell lines in this study did not vary statistically significantly between the three doses used, p**=0.004. As a result, the Melody application of a single dose is recommended as therapeutic drug and two doses application as preventive vaccine.

Properties of Calcium Phosfate Cement with Addition of Dispersant and Hydrogel

2012 ◽  
Vol 727-728 ◽  
pp. 1170-1174 ◽  
Author(s):  
J.M. Fernandes ◽  
W.T. Coelho ◽  
Mônica Beatriz Thürmer ◽  
Rafaela Silveira Vieira ◽  
Luis Alberto Santos
Keyword(s):  
Mechanical Properties ◽  
Calcium Phosphate ◽  
Mechanical Strength ◽  
Sodium Alginate ◽  
In Vitro Test ◽  
Polymeric Additives ◽  
Calcium Phosphate Bone Cement ◽  
Vitro Test ◽  
Good Biocompatibility

The calcium phosphate cements (CPCs) have attracted great interest for use in orthopedics and dentistry as replacements for damaged parts of the skeletal system,showing good biocompatibility and osseointegration. These characteristics allow its use as a bone graft.Several studies in literature have shown that the addition of polymeric additives has a strong influence on the mechanical properties of cement. The low mechanical strength is the main impediment to a broader use of calcium phosphate bone cement (CPCs) as implant material. The aim of this work was evaluate the strength of a CPC based on α-tricalcium phosphate, with polymeric additions. CPC was synthesized and sodium alginate were added (1%, 2% and 3% by weight) and ammonium polyacrylate (3%; dispersant) in aqueous solution. Specimens were molded and evaluated for density, pH, porosity, in vitro test (Simulated Body Fluid),crystalline phases and mechanical strength. The results show the increase of the mechanical properties of cement when added with sodium alginate and dispersant.

Preparation, characterization and in vitro test of composites poly-lactic acid/hydroxyapatite scaffolds for bone tissue engineering

2018 ◽  
Vol 119 ◽  
pp. 945-953 ◽  
Author(s):  
Francesco Carfì Pavia ◽  
Gioacchino Conoscenti ◽  
Silvia Greco ◽  
Vincenzo La Carrubba ◽  
Giulio Ghersi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Poly Lactic Acid ◽  
In Vitro Test ◽  
Vitro Test

scholarly journals FORMULATION, CHARACTERIZATION AND IN-VITRO EVALUATION OF FAST DISSOLVING ORAL FILMS OF CETIRIZINE HCL

2019 ◽  
Vol 9 (4-A) ◽  
pp. 122-125
Author(s):  
Yadagiri Phalguna ◽  
Haritha Pasupulati ◽  
Sandhya Rudra
Keyword(s):  
Sodium Alginate ◽  
Solvent Casting ◽  
Casting Method ◽  
Percentage Elongation ◽  
Disintegration Time ◽  
Casting Technique ◽  
Sodium Starch Glycolate ◽  
Folding Endurance ◽  
Oral Films

The predominant goal of this work is to formulate and evaluate Cetirizine HCl ODF’s the usage of Sodium starch glycolate (SSG) as superdisintegrant, Sodium alginate as polymer and Glycerol as plasticizer. Films were prepared by way of Solvent casting method and evaluated for thickness, folding endurance, percentage elongation, floor pH and disintegration time. The consequences indicate that method prepared with 17.5% combo of polymer and plasticizer was determined to be optimized. The three special formulations F1, F2 and F3 of CTZ motion pictures were organized via solvent casting technique the usage of sodium alginate as polymer, SSG as disintegrant and glycerol as plasticizer. Menthol was once used as cooling agent along with aspartame as sweetener and citric acid as a style overlaying agent. The formulation (F3) with presence of superdisintegrant and combo of polymer, plasticizer confirmed first-rate results. Keywords: Cetirizine HCl, Oral thin film, superdisintegrant, polymer, plasticizer

Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation - An Analysis of Red Blood Cell Participation

1980 ◽  
Vol 44 (01) ◽  
pp. 006-008 ◽  
Author(s):  
D Bergqvist ◽  
K-E Arfors
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
In Vitro Test ◽  
Pharmacological Agents ◽  
Vitro Test ◽  
Releasing Effect ◽  
Plug Formation

SummaryIn a model using an isolated rabbit mesenteric preparation microvessels were transected and the time until haemostatic plugs formed was registered. Perfusion of platelet rich plasma gave no haemostasis whereas whole blood did. Addition of chlorpromazine or adenosine to the whole blood significantly prolonged the time for haemostasis, and addition of ADP to the platelet rich plasma significantly shortened it. It is concluded that red cells are necessary for a normal haemostasis in this model, probably by a combination of a haemodynamic and ADP releasing effect.The fundamental role of platelets in haemostatic plug formation is unquestionable but there are still problems concerning the stimulus for this process to start. Three platelet aggregating substances have been discussed – thrombin, adenosine diphosphate (ADP) and collagen. Evidence speaking in favour of thrombin is, however, very minimal, and the discussion has to be focused on collagen and ADP. In an in vitro system using polyethylene tubings we have shown that "haemostasis" can be obtained without the presence of collagen but against these results can be argued that it is only another in vitro test for platelet aggregation (1).To be able to induce haemostasis in this model, however, the presence of red blood cells is necessary. To further study this problem we have developed a model where haemostatic plug formation can be studied in the isolated rabbit mesentery and we have briefly reported on this (2).Thus, it is possible to perfuse the vessels with whole blood as well as with platelet rich plasma (PRP) and different pharmacological agents of importance.

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