Development of Oral Cleansing Products Containing Green Tea and Centella asiatica Extracts

2021 ◽  
Vol 901 ◽  
pp. 48-54
Author(s):  
Nattaporn Amornopparattanakul ◽  
Neti Waranuch ◽  
Supaporn Lamlertthon ◽  
Wudtichai Wisuitiprot ◽  
Kornkanok Ingkaninan

“Mild” oral care products are needed by some groups of people, such as radiotherapy patients. This study developed oral cleansing products containing green tea (Camelia sinensis (L.) Kuntze) extracts with antibacterial activities and Centella asiatica (L.) Urb. extracts with a wound healing activity. We found that 3.13 mg/ml of green tea extract inhibited both Streptococcus mutans and Candida albicans, which can cause dental caries and oral candidiasis, respectively. Also, a 16 μg/ml C. asiatica extract promoted fibroblast migration in a wound healing study. The products were developed in 2 dosage forms, i.e. a solution and an impregnated gauze, which were isotonic, alcohol and volatile oil free formulations to prevent irritation and burning sensations. The products showed acceptable physical stability after testing under stressed conditions with post-test retention of antimicrobial activity.

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Hadeel A. Al-Rawaf ◽  
Sami A. Gabr ◽  
Ahmad H. Alghadir

Green tea (Camellia sinensis) has many biological activities and may promote diabetic wound healing by regulation of circulating hypoxia responsive microRNAs (HRMs) which triggers the wound repairing process in diabetic and nondiabetic wounds. Thus, in this study, the potential effects of green tea extract (GTE) on the expression of miRNAs; miR-424, miR-199a, miR-210, miR-21, and fibrogenitic markers; hydroxyproline (HPX), fibronectin (FN), and nitric oxide (NO) were evaluated in wounds of diabetic and nondiabetic rats. The animals were topically treated with vaseline, 0.6% GTE, and 5%w/w povidone iodine (standard control). HPX, FN, and NO levels and microRNAs, miR-424, miR-210, miR-199a, and miR-21, were estimated in wound tissues using colorimetric, immunoassay, and molecular PCR analysis. In vitro analysis was performed to estimate active constituents and their antioxidant activities in methanolic green teat extract (GTE). Wounds treated with green tea, a dose of 0.6, healed significantly earlier than those treated with standard vehicle and vaseline treated diabetic wounds. Higher expressions of HRMs, miR-199a, and miR-21, and lower expression of HRMs, miR-424 and miR-210, were significantly reported in tissues following treatment with green tea extract compared to standard control vehicle. The tissues also contained more collagen expressed as measures of HPX, FN, and NO and more angiogenesis, compared to wounds treated with standard control vehicle. Diabetic and nondiabetic wounds treated with green tea (0.6%) for three weeks had lesser scar width and greater re-epithelialization in shorter periods when compared to standard control vehicle. Expression of HRMs, miR-199a, miR-21, and HRMs and miR-424 and miR-210 correlated positively with HPX, fibronectin, NO, better scar formation, and tensile strength and negatively with diabetes. In addition to antidiabetic and antioxidant activities of green tea components, GTE showed angiogenesis promoting activity in diabetic wound healing. In conclusion, Camellia sinensis extracts in a dose of 0.6% significantly promote more collagen and fibronectin deposition with higher expression of NO, promoting angiogenesis process via molecular controlling of circulating hypoxia responsive microRNAs: miR-424, miR-210, miR-199a, and miR-21 in diabetic and nondiabetic wounds. Our results support a functional role of circulating hypoxia responsive microRNAs: miR-424, miR-210, miR-199a, and miR-21 as potential therapeutic targets in angiogenesis and vascular remodeling in diabetic wound healing.


2021 ◽  
Vol 16 (Supp. 1) ◽  
pp. 25-32
Author(s):  
Indra Bramanti ◽  
Annisa Hidaratri Uningojati ◽  
Dilla Asriyani ◽  
Urfa Tabtila ◽  
Fathul Muin ◽  
...  

Green tea (Camellia sinensis) has high level of flavonoids which are proven to have anti-inflammatory activity. Effect of flavonoids can be enhanced by nano-chitosan capsulation as drug carrier. Chitosan is polysaccharide derived from crustacean shells that mostly used as matrix of various drugs and plant extracts. The aim of this study was to determine the effectivity of flavonoids in green tea extract in nanochitosan capsulation towards the number of fibroblasts on proliferative phase of gingival wound healing process. Green tea was extracted, encapsulated with nano-chitosan and then made into gel. Gingiva labial of 24 male white 3-month-old Wistar rats were wounded by punch biopsy (2 mm diameter), then were treated two times a day, and were divided randomly into four groups of topical gel applications: green tea extract gel encapsulated nano-chitosan, green tea extract gel, base gel as negative control, and NSAIDs gel as positive control, starting at 0 day until 7th day. At 5th and 7th day, three rats from each group were decapitated and the mandibular gingiva was taken in order to make histology slides with hematoxylin eosin staining. Under microscope, the number of fibroblasts were examined. The data were analysed using ANOVA test with 95% confidence level. The results showed that the number of fibroblasts on proliferative phase was significantly higher than control negative (p < 0.05) and has no significant differences (p > 0.05) with control positive. In conclusion, topical application of green tea extract gel encapsulated nano-chitosan was effective to accelerate rats gingival wound healing process by increasing the fibroblasts.


2019 ◽  
Vol 3 (23) ◽  
pp. 8-13
Author(s):  
A. K. Iordanishvili

The evaluation of the effectiveness of the prevention of hyperesthesia of teeth in middle-aged people using the complex of domestic oral care products manufactured by JSC WERTEKS (St. Petersburg, Russia) has been carried out. It was shown that the best clinical result was achieved when using special toothpaste ASEPTA Plus Remineralization, mouthwash ASEPTA Parodontal Fresh and vitamin-mineral complex ASEPTA Parodontal containing coral calcium, coenzyme Q10, green tea extract and vitamins D3, C, A, B3, B6, B9 during individual oral hygiene.


Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
A Ali ◽  
X Yang ◽  
Q Shi ◽  
J Greenhaw ◽  
WF Salminen

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