scholarly journals HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH

2018 ◽  
Vol 63 (1) ◽  
pp. 3803-3810 ◽  
Author(s):  
Masoom R. Siddiqui ◽  
Saikh M. Wabaidur ◽  
Zeid A. Alothman ◽  
M.Z.A. Rafiquee ◽  
Moonis Ali Khan ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Degradation Products ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Mechanistic Approach ◽  
Stress Degradation

scholarly journals Rapid, Sensitive and Validated Ultra-Performance Liquid Chromatography/Mass Spectrometric Method for the Determination of Fenofibric Acid and its Application to Human Pharmacokinetic Study

2010 ◽  
Vol 7 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Sunil K. Dubey ◽  
Manoj S. Tomar ◽  
Anil Kumar Patni ◽  
Arshad Khuroo ◽  
Simrit Reyar ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Internal Standard ◽  
Negative Ions ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Good Precision ◽  
Mass Spectrometric Method ◽  
Fenofibric Acid

The first, rapid and sensitive ultra performance liquid chromatography mass spectrometric method for the determination of fenofibric acid, the active metabolite of fenofibrate, a lipid regulating agent, in human EDTA plasma has been developed and validated using fenofibric d6 acid as internal standard and Waters LC-MS/MS. Negative ions of fenofibric acid and fenofibric d6 acid were detected in multiple reaction-monitoring (MRM) mode. The method was validated over a concentration range of 0.176 μg/mL to 19.837 μg/mL (r ≥ 0.99). It took only 1.5 minute to analyse a sample. Intra- and inter-run precision of fenofibric acid assay at four concentrations ranged from 0.5% to 4.3% with accuracy varied from 93.1 to 108.1% indicating good precision and accuracy. Analytical recoveries of fenofibric acid and internal standard in plasma were less than 90%. This method was successfully applied for evaluation of pharmacokinetics of fenofibric acid after a single oral dose of 145 mg fenofibrate to 10 Indian healthy volunteers

Sign in / Sign up

Export Citation Format

Share Document