Quantum dots-based multiplexed immunohistochemistry of protein expression in human prostate cancer cells

Background: Prostate cancer is a heterogeneous disease and it is second deadliest malignancy in men and the most commonly diagnosed cancer among men. Current chemo-therapies are limited due to considerable side effects. Recently, many kinds of bioactive phytochemicals have contributed significantly to developing new therapies for chemo-resistant prostate cancer due to their structural diversity. Piperine, a natural alkaloid found in the fruit of black (Piper nigrum Linn) and long (Piper longum Linn), has shown antitumor activities toward various cancer cell lines. However, the antitumor effects of piperine on intrinsic and extrinsic signaling mechanisms in breast cancer has not been elucidated so far. Aim: The study aimed to assess the anticancer activity of piperine in human prostate cancer cells through intrinsic signaling pathways. Methodology: Prostate cancer (PC3) cells were treated with different concentrations of piperine (100 & 200µg/ml) to analyze Bcl-2, p53, case pase-3 and caspase-9 protein expression in PC-3 cells. Cell viability was done using MTT in order to find the optimal dose. Results: MTT assay exhibited that piperine showed cell death at the concentration of 100 and 200µg. It significantly decreased the mRNA and protein expression of anti-apoptotic proteins (Bcl-2 and p-Bcl-2) and increased the levels of p53, casepase-3 and 9 protein expression in both concentrations used. Conclusion: Our present findings show that piperine induces apoptosis in PC-3 cells by inhibition the expression of anti-apoptotic proteins with concomitant increase in the tumor suppressor proteins effectively. Hence, piperine can be considered as a potential phototherapeutic drug for the treatment of prostate cancer which may lead to clinical utility.

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Difference in Protein Expression Profile and Chemotherapy Drugs Response of Different Progression Stages of LNCaP Sublines and Other Human Prostate Cancer Cells

PLoS ONE ◽

10.1371/journal.pone.0082625 ◽

2013 ◽

Vol 8 (12) ◽

pp. e82625 ◽

Cited By ~ 6

Author(s):

Hui-Ping Lin ◽

Ching-Yu Lin ◽

Ping-Hsuan Hsiao ◽

Horng-Dar Wang ◽

Shih Sheng Jiang ◽

...

Keyword(s):

Prostate Cancer ◽

Protein Expression ◽

Cancer Cells ◽

Expression Profile ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Protein Expression Profile ◽

Chemotherapy Drugs

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Antitumor effect of reduction of 150-kDa oxygen-regulated protein expression on human prostate cancer cells

International Journal of Urology ◽

10.1046/j.1442-2042.2002.00519.x ◽

2002 ◽

Vol 9 (10) ◽

pp. 577-585 ◽

Cited By ~ 27

Author(s):

TOHRU MIYAGI ◽

OSAMU HORI ◽

KIYOSHI KOSHIDA ◽

MASAYUKI EGAWA ◽

HIROAKI KATO ◽

...

Keyword(s):

Prostate Cancer ◽

Protein Expression ◽

Cancer Cells ◽

Antitumor Effect ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells

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Proanthocyanidins from the American Cranberry (Vaccinium macrocarpon) Induce Cell Cycle Alterations in DU145 Human Prostate Cancer Cells in Vitro by Affecting the Expression of Cell Cycle-Associated Proteins

Functional Foods in Health and Disease ◽

10.31989/ffhd.v4i4.20 ◽

2014 ◽

Vol 4 (4) ◽

pp. 130

Author(s):

Joseph Kim ◽

Brendan McKeown ◽

Kunal Patel ◽

Adriana Catalli ◽

Marianna Kulka ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Cycle ◽

Protein Expression ◽

Cancer Cells ◽

Vaccinium Macrocarpon ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

American Cranberry

Background: Prostate cancer is one of the most common cancers in the world. There are genetic and environmental factors that can potentially impact the development and progression of many types of cancer, including prostate cancer. As a consequence of environmental factors, such as diet having a potential effect on the development of prostate cancer, considerable interest in the possible health benefits associated with the inclusion and consumption of certain foods in the diet exists.Context and purpose of this study: This study describes the effects of a proanthocyanidin-enriched fraction (PACs) isolated from the American cranberry (Vaccinium macrocarpon) on the behaviour of androgen-refractory (insensitive) DU145 human prostate cancer cells in vitro.Results: Following treatment of DU145 human prostate cancer cells with 25 µg/mL of PACs for six hours, PACs significantly decreased the cellular viability of DU145 cells. PACs treatment (25 µg/mL for 6 hours) of DU145 cells increased the proportion of cells in the G2-M phase of the cell cycle and decreased the proportion of cells in the G1 phase of the cell cycle. These alterations were associated with changes in cell cycle regulatory proteins and other cell cycle associated proteins. PACs increased the expression of cyclin E, cyclin D1, CDK2 and CDK4, and decreased the expression of cyclin A and cyclin B1. The protein expression level of p27 increased, and the protein expression levels of p16INK4a, p21, and pRBp107 decreased in response to PACs treatment. The protein expression level of pRBp130 was unchanged in response to PACs treatment.Conclusions: These findings demonstrate that proanthocyanidins from the American cranberry can affect the behaviour of human prostate cancer cells in vitro and further support the potential health benefits associated with cranberries.Keywords: Prostate cancer, proanthocyanidin-enriched fraction (PACs), American cranberry (Vaccinium macrocarpon)

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Magnolol Affects Cellular Proliferation, Polyamine Biosynthesis and Catabolism-Linked Protein Expression and Associated Cellular Signaling Pathways in Human Prostate Cancer Cells in vitro

Functional Foods in Health and Disease ◽

10.31989/ffhd.v5i1.163 ◽

2015 ◽

Vol 5 (1) ◽

pp. 17 ◽

Cited By ~ 4

Author(s):

Brendan T. McKeown ◽

Robert A. R. Hurta

Keyword(s):

Prostate Cancer ◽

Protein Expression ◽

Cancer Cells ◽

Cellular Proliferation ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Polyamine Biosynthesis ◽

Protein Expressions

Background: Prostate cancer is the most commonly diagnosed form of cancer in men in Canada and the United States. Both genetic and environmental factors contribute to the development and progression of many cancers, including prostate cancer.Context and purpose of this study: This study investigated the effects of magnolol, a compound found in the roots and bark of the magnolia tree Magnolia officinalis, on cellular proliferation and proliferation-linked activities of PC3 human prostate cancer cells in vitro.Results: PC3 cells exposed to magnolol at a concentration of 80 μM for 6 hours exhibited decreased protein expression of ornithine decarboxylase, a key regulator in polyamine biosynthesis, as well as affecting the expression of other proteins involved in polyamine biosynthesis and catabolism. Furthermore, protein expression of the R2 subunit of ribonucleotide reductase, a key regulatory protein associated with DNA synthesis, was significantly decreased. Finally, the MAPK (mitogen-activated protein kinase), PI3K (phosphatidylinositol 3-kinase), NFκB (nuclear factor of kappa-light-chain-enhancer of activated B cells) and AP-1 (activator protein 1) cellular signaling pathways were assayed to determine which, if any, of these pathways magnolol exposure would alter. Protein expressions of p-JNK-1 and c-jun were significantly increased while p-p38, JNK-1/2, PI3Kp85, p-PI3Kp85, p-Akt, NFκBp65, p-IκBα and IκBα protein expressions were significantly decreased.Conclusions: These alterations further support the anti-proliferative effects of magnolol on PC3 human prostate cancer cells in vitro and suggest that magnolol may have potential as a novel anti-prostate cancer agent.Key Words: prostate cancer cells, magnolol, polyamines, MAPK, PI3K, NFκB

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