Vitamin E reduces placental oxidative stress and improves pregnancy outcome in pregnant rats at high altitude

2019 ◽  
Vol 4 (2) ◽  
pp. 32
Author(s):  
MohammedE M. Khalid ◽  
HumedaS Ahmed ◽  
OsamaM Osman ◽  
FahaidH AL Hashem
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
High Altitude ◽  
Pregnancy Outcome ◽  
Pregnant Rats ◽  
Placental Oxidative Stress

scholarly journals Gebe Ratlara uygulanan Akrilamid ve E Vitaminin Plasenta Dokusu Üzerine Olası Etkilerinin Araştırılması

2017 ◽  
Vol 5 (4) ◽  
pp. 349
Author(s):  
Mehmet Erman Erdemli ◽  
Eyüp Altınöz ◽  
Zeynep Aksungur ◽  
Zümrüt Doğan ◽  
Harika Gözükara Bağ ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Total Antioxidant Capacity ◽  
Corn Oil ◽  
Reduced Glutathione ◽  
Control Group ◽  
Vaginal Smear ◽  
Protective Agent ◽  
Pregnant Rats ◽  
Total Antioxidant

Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.

scholarly journals The Effect of Administration of Omega-3 and Vitamin E on the Levels of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) in Preeclampsia White Rats (Rattus Norvegicus)

2021 ◽  
Vol 8 (12) ◽  
pp. 7-15
Author(s):  
Dina Taufia ◽  
Syamel Muhammad ◽  
Arni Amir
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Vitamin E ◽  
Control Group ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Pregnant Rats ◽  
White Rats ◽  
Significant Difference ◽  
One Way Anova

Background: Preeclampsia is a disease with a variety of theories that describe the uncertainty of the pathophysiology. According to the oxidative stress theory, preeclampsia originates from the failure of trophoblast invasion during the implantation process, causing ischemia and placental hypoxia, which in turn causes cell damage, including placental endothelial cell dysfunction. Omega-3 fatty acids and vitamin E have an important role in preventing preeclampsia. Omega-3 fatty acids play an important role in maintaining cell membranes and anti-inflammatory processes. At the same time, vitamin E acts as a fat-soluble antioxidant that can prevent oxidative stress, inhibit proinflammatory cytokines, and protect fatty acids from oxidation. Aim: The purpose of this study was to determine the effect of omega-3 and vitamin E on the level of ROS and NO in pregnant rats with preeclampsia models. Method & Material: This type of research is experimental with Post-Test Only Control Group Design. The sample consisted of 35 pregnant rats, which were divided into five groups. On the 19th day, blood serum was taken to check the levels of ROS and NO. The measuring instrument used is a spectrophotometer with the ELISA method. Data were analyzed using the Shapiro Wilks normality test. After the parametric test was completed, the hypothesis was tested using one-way ANOVA. Results: The average levels of ROS in each group were K- : 121,684 ng/L, K+ : 143,885 ng/L, P1 : 136,250 ng/L, P2 : 132.433 ng/L, and P3 : 122,993 ng/L. The average levels of NO obtained were K-: 29,502 ng/L, K+: 26,053 ng/L, P1: 27,250 ng/L, P2: 27,555 ng/L, and P3: 32,278 ng/L. The results of one-way ANOVA analysis showed that the administration of omega-3 and vitamin E had a significant difference between the control and treatment groups, both at levels of ROS (p=0.001) and levels of NO (p=0.001). Conclusion: The administration of omega-3, vitamin E, and omega-3 plus vitamin E can reduce ROS levels in pregnant rats with preeclampsia models. There is an increase of NO levels only in the administration of omega-3 plus vitamin E. Keywords: [Omega-3, Vitamin E, Oxidative stress, ROS, NO, Preeclampsia].

scholarly journals Omega-3 and Vitamin E Supplementation Effect to Rattus Norvegicus Placental Apoptosis Marker: Pre-Eclampsia Model

2021 ◽  
Vol 6 (4) ◽  
pp. 261-268
Author(s):  
Yesi Gusti ◽  
Arni Amir ◽  
Gusti Revilla
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Placental Tissue ◽  
Control Group ◽  
Omega 3 ◽  
Control Group Design ◽  
Pregnant Rats ◽  
Post Test ◽  
The Mean ◽  
Animal House

Background: Pre-eclampsia has been associated with the increased of placental apoptosis caused by oxidative stress. Omega-3 fatty acids and vitamin E had beneficial function to maintain cell membranes, prevent oxidative stress and inhibit the production of proinflammatory cytokines. The purpose of the study was to determine the effect of omega-3 and vitamin E supplement to BCL-2 and BAX on PE rats model. Methods: This research has been worked out at animal house and Biomedical Laboratory of the Medical Faculty Andalas University. The design of this research was experimental study with post test only control group design. There were 35 pregnant rats were divided into 5 groups. Group K- without treatment, group K+ was given L-NAME, group P1 was given L-NAME + omega-3, group P2 was given L-NAME + vitamin E and group P3 was given L-NAME + omega-3 + vitamin E. The L-NAME dose was 50mg/kg/day, At the 19th day of gestation, the pregnancy was terminated and the placental tissue was collected for examining BCL-2 and BAX levels by using ELISA kit. The data were analyzed by one way ANOVA and post hoc test LSD. Results: The mean levels of BCL-2 in each group were K- =62.49 ng/mg, K+ =50.64 ng/mg, P1 =58.79 ng/mg, P2 =53.70 ng/mg and P3 =70.10 ng/mg. The mean levels of BAX were K- =72.06 ng/mg, K+ =78.61 ng/mg, P1 =73.71 ng/mg, P2 =74.31 ng/mg and P3 =71.63 ng/mg. The analysis results concluded that there were significant differences between omega-3 and vitamin E supplements on BCL-2 levels (p=0.000) and BAX levels (p=0.001). Conclusion: Omega-3 (DHA 120 mg, EPA 180 mg) and vitamin E (α-tocopherol 300 iu) suplement increased BCL-2 level and decreased BAX on pre-eclampsia rats model. Keywords: Omega-3, Vitamin E, BCL-2, BAX, Pre-eclampsia.

scholarly journals Potential Therapeutic Effects of Vitamin E and C on Placental Oxidative Stress Induced by Nicotine: An In Vitro Evidence

2010 ◽  
Vol 4 ◽  
pp. 77-82 ◽  
Author(s):  
Chiara Gallo ◽  
Paolo Renzi ◽  
Stefano Loizzo ◽  
Alberto Loizzo ◽  
Sonia Piacente ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Therapeutic Effects ◽  
Vitamin E And C ◽  
Placental Oxidative Stress

Hypoxia-induced oxidative stress in high altitude population: impact of coenzyme Q10 supplementation

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ayman R. Biuomy ◽  
Fatma S. H. Oraby ◽  
Eman A. Khalifa ◽  
Hanaa A. El-Sherif ◽  
Jihan Hussein ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Placebo Group ◽  
Vitamin E ◽  
High Altitude ◽  
Sea Level ◽  
Coenzyme Q10 ◽  
High Performance ◽  
Base Line ◽  
Antioxidant Parameters ◽  
Coq10 Supplementation

Abstract Objective The aim of this study was to investigate the effect of coenzyme Q10 (CoQ10) supplementation on oxidative stress engendered from hypoxia in population live at high altitude. Methods This is an intervention study in which 50 females of volunteers population-36 of them who live at high altitude compared with the placebo group (14 from the total population that live at sea level). Blood samples were collected in -anticoagulant tubes from control and high altitude before and after CoQ10 supplementation (150 mg/day for 2, 4 and 8 weeks). Plasma was separated and used for the determination of malondialdehyde (MDA), nitric oxide (NOx), total antioxidant capacity (TAC), paraoxonase (PON1) by spectrophotometer, CoQ10 and vitamin E by high performance liquid chromatography (HPLC). Results Our results appeared that TAC, PON1, vitamin E and CoQ10 concentrations were significantly decreased in population at high altitude at base line compared to placebo group population at sea level. Whereas, administration of CoQ10 attenuated all measured parameters especially after eight weeks of administration. Conclusion We concluded that coenzyme Q10 supplement at a dose of 150 mg/day has a powerful effect in oxidative stress parameters and increased antioxidant parameters included vitamin E in population with hypoxia after 4 and 8 weeks. So that supplementation positively affects oxidative stress and is recommended CoQ10 supplementation in population who live at high altitude.

Changes in lipid peroxidation during pregnancy and after delivery in rats: effect of pinealectomy

Reproduction ◽  
2000 ◽  
pp. 143-149 ◽  
Author(s):  
RM Sainz ◽  
RJ Reiter ◽  
JC Mayo ◽  
J Cabrera ◽  
DX Tan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Energy Demand ◽  
Physiological State ◽  
High Energy ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Oxygen Requirement ◽  
Pregnant Rats

Pregnancy is a physiological state accompanied by a high energy demand of many bodily functions and an increased oxygen requirement. Because of the increased intake and utilization of oxygen, increased levels of oxidative stress would be expected. In the present study, the degree of lipid peroxidation was examined in different tissues from non-pregnant and pregnant rats after the delivery of their young. Melatonin and other indole metabolites are known to be direct free radical scavengers and indirect antioxidants. Thus the effect of pinealectomy at 1 month before pregnancy on the accumulation of lipid damage was investigated in non-pregnant and pregnant rats after the delivery of their young. Malonaldehyde and 4-hydroxyalkenal concentrations were measured in the lung, uterus, liver, brain, kidney, thymus and spleen from intact and pinealectomized pregnant rats soon after birth of their young and at 14 and 21 days after delivery. The same parameters were also evaluated in intact and pinealectomized non-pregnant rats. Shortly after delivery, lipid oxidative damage was increased in lung, uterus, brain, kidney and thymus of the mothers. No differences were detected in liver and spleen. Pinealectomy enhanced this effect in the uterus and lung. It is concluded that during pregnancy high levels of oxidative stress induce an increase in oxidative damage to lipids, which in some cases is inhibited by the antioxidative actions of pineal indoles.

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