scholarly journals Bacterial Examination of Wild and Cultured Fish Present in the Same Aquatic Ecosystem, and the Antibiotic Resistance of the Isolated Bacteria

10.4194/ga385 ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Ahmet Bingöl ◽  
Akif Er ◽  
Zeynep Zehra İpek ◽  
Şevki Kayış
Keyword(s):  
Antibiotic Resistance ◽  
Clavulanic Acid ◽  
Bacterial Species ◽  
Universal Primers ◽  
Bacterial Isolates ◽  
Yersinia Ruckeri ◽  
Monthly Basis ◽  
Aeromonas Sobria ◽  
Cultured Fish ◽  
Amoxicillin Clavulanic Acid

This study investigated the bacteria present in natural and cultured fish species from the same aquatic system, and difference of their antibiotic resistance. A total of 129 fish, Georgian shemaya (Alburnus derjugini), black sea salmon (Salmo labrax) and rainbow trout (Oncorhynchus mykiss), were sampled on a monthly basis between the months of October 2016 and September 2017 in Kürtün Dam Lake. A total of 41 bacterial isolates were isolated from the fishes. The bacterial species were identified by molecular methods (PCR) using universal primers for bacteria. Acinetobacter lwoffii, Acinetobacter sp., Aeromonas sobria, and Pseudomonas sp. were isolated from both wild and cultured fish. Yersinia ruckeri was isolated from cultured fish, which showed severe mortality rate and typical symptoms. Various antibiotics including ampicillin (AMP10µg), gentamicin (CN10 µg), oxytetracycline (T30 µg), amoxicillin/clavulanic acid (AMC10µg), enrofloxacin (ENR5µg), trimethoprim/sulfamethoxazol (TMP-SMZ25µg), florfenicol (FFC30µg), sulfamethoxazol (SMZ25µg) and erythromycin (E15µg) were used for determination of the bacterial resistances. The highest resistances were determined against ampicillin (56%), sulfamethoxazol (46.3%) and oxytetracycline (34.1) in all bacterial isolates. While the bacteria isolated from wild fish did not show resistance to enrofloxacin and amoxicillin/clavulanic acid, those from cultured fish did not show resistance to gentamicin and erythromycin.

scholarly journals STUDY OF ANTIBIOTIC-RESISTANCE AND SENSITIVITY PATTERN IN A TERTIARY CARE TEACHING HOSPITAL

2021 ◽  
Vol 9 (12) ◽  
pp. 161-168
Author(s):  
Sailajapriyadarsini Parlapalli ◽  
◽  
V.V Padmavathy ◽  
Gunapati Rama Mohan Reddy ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Clavulanic Acid ◽  
Body Fluid ◽  
Tertiary Care ◽  
Antibiotic Use ◽  
Study Data ◽  
Urine Samples ◽  
Sensitivity Pattern ◽  
Amoxicillin Clavulanic Acid

Introduction: Antibiotics are helpful in bacterial infection control and management. Irrational antibiotic use leads to resistance, which puts modern medicines achievements at risk. This study aims to know antibiotic resistance and sensitivity patterns and to assess possible factors that favor the development of antibiotic resistance. Methods: It is a retrospective observational study. Data collected from June 2019 to May 2020 from the culture & sensitivity records of various clinical isolates at the Microbiology department of SMC, Vijayawada, and analyzed by descriptive statistics. Results: Total samples collected 2905. Only746 samples showed significant growth. Most commonly, isolated organisms were from Pus and Body fluids Staphylococcus aureus (31.16%), Klebsiella (23.02%), from blood samples Staphylococcus aureus (44%,) Klebsiella (32%), from urine samples Escherichia coli (27.31%),Staphylococcus aureus (16.66%). Bacteria isolated from, Blood and Pus & body fluid samples showed more resistance to Amoxicillin + Clavulanic acid, 60% &58.60%, and urine samples showed more resistance to Norfloxacin69.44%. Conclusion: In this study, bacteria isolated from, blood, pus, and body fluid samples showed more resistance to Amoxicillin + Clavulanic acid, from urine samples showed more resistance to Norfloxacin. Parenterally used drugs like aminoglycosides and vancomycin have retained their sensitivity but bacteria also showed resistance to Imipenem, Vancomycin, new combinations like Cefoperazone + Sulbactam. A culture & sensitivity test and following antibiotic stewardship guidelines before prescribing antibiotics will give good results to prevent antibiotic resistance.

scholarly journals In Vitro Activity of Moxifloxacin against Bacteria Isolated from Odontogenic Abscesses

2002 ◽  
Vol 46 (12) ◽  
pp. 4019-4021 ◽  
Author(s):  
Ingo Sobottka ◽  
Georg Cachovan ◽  
Enno Stürenburg ◽  
M. Oliver Ahlers ◽  
Rainer Laufs ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Antimicrobial Susceptibility ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Bacterial Isolates ◽  
Amoxicillin Clavulanic Acid ◽  
Viridans Group Streptococci

ABSTRACT We evaluated the antimicrobial susceptibility of 87 pathogens isolated from 37 patients with odontogenic abscesses. The most prevalent bacteria were viridans group streptococci and Prevotella species. Considering all bacterial isolates, 100% were susceptible to amoxicillin-clavulanic acid, 98% were susceptible to moxifloxacin and to levofloxacin, 76% were susceptible to doxycycline, 75% were susceptible to clindamycin, and 69% were susceptible to penicillin.

scholarly journals Antibiotic Resistance in Haemophilus influenzae Decreased, except for β-Lactamase-Negative Amoxicillin-Resistant Isolates, in Parallel with Community Antibiotic Consumption in Spain from 1997 to 2007

2008 ◽  
Vol 52 (8) ◽  
pp. 2760-2766 ◽  
Author(s):  
Silvia García-Cobos ◽  
José Campos ◽  
Emilia Cercenado ◽  
Federico Román ◽  
Edurne Lázaro ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Haemophilus Influenzae ◽  
Clavulanic Acid ◽  
Antimicrobial Agents ◽  
Geographic Area ◽  
Antibiotic Consumption ◽  
Control Group ◽  
Promoter Regions ◽  
Geometric Means ◽  
Amoxicillin Clavulanic Acid

ABSTRACT The susceptibility to 14 antimicrobial agents and the mechanisms of aminopenicillin resistance were studied in 197 clinical isolates of Haemophilus influenzae—109 isolated in 2007 (study group) and 88 isolated in 1997 (control group). Community antibiotic consumption trends were also examined. H. influenzae strains were consecutively isolated from the same geographic area, mostly from respiratory specimens from children and adults. Overall, amoxicillin resistance decreased by 8.4% (from 38.6 to 30.2%). β-Lactamase production decreased by 15.6% (from 33 to 17.4%, P = 0.01), but amoxicillin resistance without β-lactamase production increased by 7.1% (from 5.7 to 12.8%). All β-lactamase-positive isolates were TEM-1, but five different promoter regions were identified, with Pdel being the most prevalent in both years, and Prpt being associated with the highest amoxicillin resistance. A new promoter consisting of a double repeat of 54 bp was detected. Community consumption of most antibiotics decreased, as did the geometric means of their MICs, but amoxicillin-clavulanic acid and azithromycin consumption increased by ca. 60%. For amoxicillin-clavulanic acid, a 14.2% increase in the population with an MIC of 2 to 4 μg/ml (P = 0.02) was observed; for azithromycin, a 21.2% increase in the population with an MIC of 2 to 8 μg/ml (P = 0.0005) was observed. In both periods, the most common gBLNAR (i.e., H. influenzae isolates with mutations in the ftsI gene as previously defined) patterns were IIc and IIb. Community consumption of trimethoprim-sulfamethoxazole decreased by 54%, while resistance decreased from 50 to 34.9% (P = 0.04). Antibiotic resistance in H. influenzae decreased in Spain from 1997 to 2007, but surveillance should be maintained since new forms of resistances may be developing.

scholarly journals Susceptibility of Incisional Site Staphylococcus spp. to Three Common Perioperative Antimicrobials in Dogs Undergoing Clean Orthopaedic Procedures

VCOT Open ◽  
2020 ◽  
Vol 03 (02) ◽  
pp. e158-e163
Author(s):  
Po-Yen Chou ◽  
Duane Robinson ◽  
Amy S. Kapatkin ◽  
Steven E. Epstein ◽  
Michelle A. Giuffrida ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Prospective Observational Study ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Coagulase Negative Staphylococci ◽  
Bacillus Spp ◽  
Amoxicillin Clavulanic Acid ◽  
Healthy Dogs ◽  
Staphylococcus Spp ◽  
Coagulase Positive Staphylococci

Abstract Objectives The aim of this study was to evaluate the susceptibility pattern of the incisional site Staphylococcus spp. to three commonly used perioperative antimicrobials in dogs. Materials and Methods A cohort of client-owned dogs was included in this prospective observational study. After induction of general anaesthesia and hair clipping, culture samples were collected from the incisional site of healthy dogs before orthopaedic surgery. The isolated colonies were identified. The susceptibility pattern of staphylococcal isolates was determined using Clinical and Laboratory Standard Institute standards. The staphylococcal susceptibilities to amoxicillin/clavulanic acid and cefpodoxime were compared with cefazolin using two-sided equality of proportions test. Results A total of 391 bacterial isolates were identified in 115 dogs. Bacillus spp. (n = 200) and Staphylococcus spp. (n = 95) were the most common bacteria isolated. Coagulase- positive and -negative staphylococci were isolated in 44 and 38% of dogs respectively. Cefazolin, amoxicillin/clavulanic acid and cefpodoxime staphylococcal susceptibilities were 68, 77 and 76% respectively. There were no significant differences in Staphylococcus spp. susceptibility between amoxicillin/clavulanic acid or cefpodoxime and cefazolin (p = 0.19, p = 0.25). Thirty-six per cent of coagulase-negative staphylococci and 6% of coagulase-positive staphylococci isolated were methicillin resistant. Conclusion Cefazolin, amoxicillin/clavulanic acid and cefpodoxime had similar susceptibilities for staphylococcal isolates. Routine use of amoxicillin/clavulanic acid or cefpodoxime in replace of cefazolin should be practiced with caution.

scholarly journals Phenotypic Detection of Extended Spectrum Beta-lactamase Resistance of Escherichia coli from Patients Attending Selected Healthcare Facilities in Nasarawa State, Nigeria

2019 ◽  
pp. 1-10
Author(s):  
R. H. Abimiku ◽  
Y. B, Ngwai ◽  
I. H. Nkene ◽  
B. E. Bassey ◽  
P. A. Tsaku ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Clavulanic Acid ◽  
Age Groups ◽  
Beta Lactamase ◽  
Healthcare Facilities ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Microbiological Methods ◽  
Amoxicillin Clavulanic Acid

Aims: This study investigated the phenotypic detection of extended spectrum beta-lactamase resistance of diarrheagenic E. coli isolated from diarrheic patients attending some major health facilities in Nasarawa State, Nigeria. Place and Duration of Study: Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria; between December, 2017 to March, 2019. Methodology: A total of 207 confirmed E. coli isolates from loose stool samples of patients with suspected cases of diarrhea (69 from Federal Medical Centre Keffi [MCK] 69 from General Hospital Akwanga [GHA] and 69 from Dalhatu Araf Specialist Hospital Lafia [DASHL]) were included in this study. Results: E. coli was isolated and identified using standard microbiological methods. The antibiotic susceptibility testing for the isolates was carried out and interpreted in accordance with Clinical and Laboratory Standards Institute protocol. Phenotypic detection of ESBL production in isolates resistant to ciprofloxacin, cefotaxime and ceftazidime) was carried out using double disc synergy test. The occurrence of E. coli was 100% in all the hospitals. Age groups 0-5 and 6-10 years have the highest occurrence than age group 35 – >45 years. Isolates from DASHL were more resistant to amoxicillin/clavulanic acid (86.9%), Streptomycin (75.0%) and sulphamethoxazole/trimethoprim (68.1%), isolates from FMCK were more resistant to amoxicillin/clavulanic acid (84.1%), sulphamethoxazole/trimethoprim (69.6%), isolates from GHA were more resistant to amoxicillin/clavulanic acid (85.5%) and sulphamethoxazole/trimethoprim (73.0%). Multiple antibiotic resistance (MAR) was observed with the order of occurrence: FMCK (98.6%) > DASHL (92.8%) > GHA (89.9%). The most common MAR index of 0.2 in DASHL was 0.4 (20.3%); FMCK was 0.4 (15.9%) and GHA was 0.3 (17.4%). The order of occurrence of classes of antibiotic resistance in E. coli isolates in DASHL was MDR (84.0%) ˃ XDR(7.2%) > PDR and NMDR (4.3%); in FMCK was MDR (91.3%) ˃ XDR(4.3%) ˃ NMDR (2.9%) and PDR(1.4%); and in GHA was MDR (88.8%) ˃ NMDR(5.8%) > XDR and PDR(2.9%). Detection rate of ESBL was 53.6% (30/207), distributed in relation to the location as DASHL (60.0%), FMCK (50.0%) and GHA (52.6%). Conclusion: Most of the isolates from the study locations were antibiotic resistance. Further studies on molecular detection of ESBL, diversity and characterization of the E. coli into pathotypes are ongoing.

scholarly journals Sensitivity to Antibiotics of Staphylococcus Aureus Strains, Able to Biofilm Formation

2021 ◽  
Vol 6 (3) ◽  
pp. 226-231
Author(s):  
A. O. Vashchenko ◽  
◽  
Yu. S. Voronkova ◽  
A. I. Vinnikov ◽  
T. N. Shevchenko ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Clavulanic Acid ◽  
Rapid Determination ◽  
Diffusion Method ◽  
Upper Respiratory Tract ◽  
Isolation And Identification ◽  
Methicillin Resistant ◽  
Opportunistic Bacteria ◽  
Amoxicillin Clavulanic Acid

The problem of respiratory diseases associated with staphylococcal carriers is considered to be one of the most acute in modern society due to its high prevalence and problems with treatment due to the high resistance of these bacteria to antibiotics. The purpose of the study was to determine the sensitivity of Staphylococcus aureus strains, able to form biofilm, isolated from the upper respiratory tract of human. Materials and methods. Bacteriological methods of isolation and identification of staphylococci, the method of rapid determination of the ability to form biofilm on a tablet and the disk-diffusion method of determining the susceptibility to antibiotics were used to perform the research. As a result of the conducted researches it was established that in the structure of pathogens of exacerbations of seasonal rhinitis S. aureus prevailed. It was isolated from 34 samples of biological material from the nose, which accounted for 69.4% of all examined cases of rhinitis. Staphylococcus spp. (6.1%) and Streptococcus spp. (24.5%) among the isolated strains of S. aureus, 27 (79.4%) had the ability to form a biofilm. A feature associated with antibiotic resistance is the ability of strains of microorganisms to form a biofilm, in which they acquire enhanced ability to survive under adverse environmental factors, including the use of drugs. Results and discussion. In our research we showed that more than 80% of film-forming strains were found to be susceptible to ciprofloxacin, amoxicillin / clavulanic acid and azithromycin. 4 (14.8%) are methicillin-resistant. Resistance to methicillin was detected simultaneously with resistance to ciprofloxacin (3 cases) and to azithromycin (2 cases), 1 strain was resistant to gentamicin and 2 – to tetracycline. 1 of the methicillin-resistant strains of S. aureus was multidrug-resistant (showed resistance to all studied antibiotics). Among non-biofilm strains, more than 80% of the strains were also sensitive to ciprofloxacin, amoxicillin / clavulanic acid and azithromycin. Sensitivity to tetracycline was low in both groups (42-63% sensitive). Conclusion. The prevalence of antibiotic resistance among clinical strains of opportunistic bacteria, unfortunately, shows an increasing trend. This requires constant monitoring of susceptibility to antimicrobial drugs in order to develop schemes of rational antibiotic therapy, taking into account the individualized approach to patients. In this sense, it is promising when studying the biological properties of clinical isolates to determine not only the resistance to antibiotics, but also their ability to form a biofilm

scholarly journals Drug resistance and susceptibility testing of Gram negative bacterial isolates from healthy cattle with different β – Lactam resistance Phenotypes from Shandong province China

2023 ◽  
Vol 83 ◽  
Author(s):  
S. Arbab ◽  
H. Ullah ◽  
X. Wei ◽  
W. Wang ◽  
S. U. Ahmad ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Shandong Province ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Healthy Cattle ◽  
Shigella Spp ◽  
Amoxicillin Clavulanic Acid ◽  
Klebsiella Spp

Abstract The objective of this study was to evaluate the effectiveness of common antibiotics against different microorganisms in apparently healthy cattle in Shandong province and its suburb. A total of 220 nasal swab samples were collected and cultured for bacteriological evaluation. All the bacteria isolates after preliminary identification were subjected to antibiogram studies following disc diffusion method. It was found in the study that E. coli is the most commonly associated isolate (21%), followed by Klebsiella spp. (18%), Pseudomonas aeruginosa (13%), Salmonella spp. (15%), Shigella spp (12%), and Proteus spp (11%). While the antibiogram studies reveled that highest number of bacterial isolates showed resistance to Ampicillin (95%), followed by Augmentin (91%), Cefuroxime (85%) and Tetracycline (95%) of (Escherichia coli and Klebsiella spp). In the case of pseudomonas spp. and Salmonella the highest resistance was showed by Ampicillin (90%) followed by Amoxicillin + Clavulanic Acid (80%), Cefixime (90%), and Erythromycin (80%). In Shigella spp and Salmonella spp highest resistance was showed by Amoxicillin, Ceftazidime, Augmentin (60%), and Amoxicillin + Clavulanic Acid (50%). It is concluded that in vitro antibiogram studies of bacterial isolates revealed higher resistance for Ampicillin, Augmentin, Cefuroxime, Cefixime, Tetracycline, Erythromycin, and Amoxicillin + Clavulanic Acid. The high multiple Antibiotics resistance indexes (MARI) observed in all the isolates in this study ranging from 0.6 to 0.9. MARI value of >0.2 is suggests multiple antibiotic resistant bacteria and indicate presence of highly resistant bacteria.

scholarly journals Comparison of beta-lactamase genes in clinical and food bacterial isolates in India

2009 ◽  
Vol 3 (08) ◽  
pp. 593-598 ◽  
Author(s):  
Mohd. Shahid ◽  
Abida Malik ◽  
Mohd. Adil ◽  
Noor Jahan ◽  
Ritu Malik
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Species ◽  
Enteric Bacteria ◽  
Generation Cephalosporin ◽  
Clinical Isolates ◽  
Third Generation ◽  
Bacterial Isolates ◽  
Food Borne ◽  
Resistance Rates ◽  
Third Generation Cephalosporins

BACKGROUND: The present study aimed to determine the occurrence of human disease-causing enteric bacteria on raw vegetables, fruits, meats, and milk products sold in Indian markets. The study further aimed to analyze antibiotic resistance rates and the presence of blaCTX-M, blaTEM, blaSHV, and blaAmpC. METHODOLOGY: Twenty-three food-borne and 23 clinical isolates were compared for antibiotic resistance rates and the presence of blaCTX-M, blaTEM, blaSHV, and blaAmpC. Swabs were taken from unwashed and washed food items, as well as from some chopped food specimens, and inoculated on appropriate culture medium. Bacterial isolates were identified, antibiotic susceptibility was performed, and bla genes were detected by PCR. RESULTS: Thirty-eight bacterial isolates were obtained from the food specimens, of which 36 (94.7%) were Gram-negative and two (5.3%) were Gram-positive bacterial species. Klebsiella pneumoniae was the most prevalent (52.6%; 20/38) bacterial species isolated, followed by Citrobacter koseri (18.4%; 7/38). In food isolates, the majority of the isolates were resistant to gentamicin (33.3%) followed by amikacin (11.1%). Resistance to a third-generation cephalosporin was noticed in only 5.6% isolates. However, in clinical isolates, maximal resistance was noticed against third-generation cephalosporins followed by ofloxacin in 91.3% and 86.9% isolates, respectively, and resistance to gentamicin and amikacin was noticed in 78.3% and 52.2% isolates, respectively. The presence of blaCTX-M, blaTEM, blaSHV, and blaAmpC in clinical isolates was noticed in 52.2%, 60.9%, 21.7%, and 43.5%, respectively. None of the isolates from food showed the presence of any of the above-cited genes. CONCLUSIONS: Probably bla genes have not yet disseminated to raw-food vegetation in India.

Antibiotic Molecules Efficacy in Pediatric Bacterial Infections

2018 ◽  
Vol 69 (4) ◽  
pp. 915-920
Author(s):  
Petronela Cristina Chiriac ◽  
Vladimir Poroch ◽  
Alina Mihaela Pascu ◽  
Mircea Daniel Hogea ◽  
Ileana Antohe ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Klebsiella Pneumoniae ◽  
Clavulanic Acid ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Penicillin G ◽  
Acute Therapy ◽  
Amoxicillin Clavulanic Acid

The antibiotic resistance of microorganisms involved in pediatric infections represents a significant cause of healthcare-associated infections (HAIs), and is also a matter of management, requiring specific intervention. The aim of the study was to evaluate the efficacy of some antibiotic molecules on pathogens isolated from patients admitted in a pediatric hospital. We carried out a descriptive study on a group of 411 patients admitted to the Sf. MariaClinical Emergency Hospital for Children Iasi, between January 1st and March 31st, 2016. Bacterial infections were most prevalent in the age group of 0-1 year (54.98% of the total isolates). Most affected by multidrug-resistant bacterial infections services were: general pediatrics (24.08% of the total isolates), then the intensive care unit (19.95%), surgical wards (14.84%), and acute therapy (11.43%). The germs were isolated from pathological samples: most often pus (23.85%), hypo-pharyngeal aspiration (21.65%), conjunctival secretion (12.42%), and ear secretion (9.48%). Penicillin G and oxacillin were inefficient in 30.26% of the Staphylococcus aureus strains, while erythromycin in 18.42%. Antibiotic resistance of Streptococcus pneumoniae was observed for penicillin G in 7.14% of the strains, while for erythromycin in 13.09%. Klebsiella pneumoniae strains were resistant to amoxicillin + clavulanic acid in 35.85% of the cases, and to cefuroxime, ceftazidime, ceftriaxone, cefepime in 33.96%. Our study highlighted that Staphylococcus aureus was resistant to penicillin G and oxacillin in more than one-third of the isolates, Streptococcus pneumoniae was resistant to penicillin G and erythromycin, and Klebsiella pneumoniae to amoxicillin + clavulanic acid and 1st, 2nd and 3rd generation cephalosporins. Continuing antibioresistance monitoring is crucial in order to promote appropriate guidelines in antibiotic prescription, which could result in decreasing HAIs� rates.

scholarly journals Monitoring of antibiotic resistance in isolates of Actinobacillus pleuropneumoniaein the Czech Republic between 2001 and 2003

2012 ◽  
Vol 50 (No. 5) ◽  
pp. 181-185 ◽  
Author(s):  
K. Nedbalcova ◽  
P. Satran ◽  
Z. Jaglic ◽  
R. Ondriasova ◽  
Z. Kucerova
Keyword(s):  
Antibiotic Resistance ◽  
Czech Republic ◽  
Clavulanic Acid ◽  
Actinobacillus Pleuropneumoniae ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
High Increase ◽  
The Czech Republic ◽  
Amoxicillin Clavulanic Acid

During the period of 2001 and 2003 a total of 238 isolates of Actinobacillus pleuropneumoniae obtained from 26 pig herds in the Czech Republic (2001 – 73 isolates, 2002 – 110 isolates, 2003 – 55 isolates) were examined for antibiotic resistance by disc diffusion method. Resistance to erythromycin (95.5% to 98.6%), streptomycin (76.4% to 82.7%) and tiamulin (72.7%) was detected most frequently over the whole monitored period. High increase in percentage of resistant isolates to tetracycline (2001 – 11.0%, 2002 – 36.4%, 2003 – 81.8%), doxycycline (2001 – 9.6%, 2002 – 34.6%, 2003 – 61.8%), nalidixic acid (2001 – 2.7%, 2002 – 14.5%, 2003 – 45.5%), and norfloxacin (2001 – 0%, 2002 – 7.3%, 2003 – 34.6%) was found, while differences in resistance to cephalotine (2001 – 1.4%, 2002 – 7.3%, 2003 – 9.1%), cotrimoxazol (2001 – 4.1%, 2002 – 10.0%, 2003 – 7.3%), amoxicillin (2001 – 12.3%, 2002 – 12.7%, 2003 – 10.9%) and amoxicillin-clavulanic acid (2001 – 9.6%, 2002 – 9.1%, 2003 – 10.9%) were small. Prevalence of isolates resistant to sulfasomidine was detected decreasing (2001 – 43.8%, 2002 – 47.3% and 2003 – 29.1%).

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