scholarly journals GENETIC CONNECTIVITY OF MULLETS (Mugil liza) BETWEEN RODRIGO DE FREITAS LAGOON AND A CONSERVATION UNIT IN STATE OF RIO DE JANEIRO, SOUTHEASTERN BRAZIL

2021 ◽  
Vol 25 (03) ◽  
pp. 757-762
Author(s):  
Anderson Vilasboa de Vasconcellos ◽  
◽  
Livia Bonetti Villela ◽  
Denise Borges dos Santos Dias ◽  
Karina Alessandra Morelli ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Rio De Janeiro ◽  
Genetic Connectivity ◽  
Southeastern Brazil ◽  
Conservation Unit ◽  
High Similarity ◽  
Allelic Frequencies ◽  
Natural Monument ◽  
Shared Alleles

We analyzed the genetic connectivity between mullets (Mugil liza) captured around the protected Natural Monument of Cagarras Islands (MoNa Cagarras) and inside Rodrigo de Freitas Lagoon, using microsatellite markers polymorphisms. Our data revealed the occurrence of 31 shared alleles (from 41 sampled), a high similarity in both allelic frequencies and genetic diversity and lack of differentiation between collection points (FST = 0.000, p > 0.05, STRUCTURE best estimative K = 1), results which, analyzed together, are strongly indicative of panmixia. We conclude that individuals collected inside the Rodrigo de Freitas Lagoon are genetically similar to those individuals collected around MoNa Cagarras. Given the importance of estuaries for the reproduction and development of individuals of M. liza, it is recommended that the Rodrigo de Freitas Lagoon to be managed in order to maintain genetic connectivity and diversity between the two ecosystems.

scholarly journals Yellow Fever Virus Reemergence and Spread in Southeast Brazil, 2016–2019

2019 ◽  
Vol 94 (1) ◽  
Author(s):  
Marta Giovanetti ◽  
Marcos Cesar Lima de Mendonça ◽  
Vagner Fonseca ◽  
Maria Angélica Mares-Guia ◽  
Allison Fabri ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Yellow Fever ◽  
Rio De Janeiro ◽  
Nonhuman Primates ◽  
Yellow Fever Virus ◽  
Genomic Data ◽  
Fever Virus ◽  
Southeastern Brazil ◽  
Espirito Santo ◽  
Southeastern Region

ABSTRACT The recent reemergence of yellow fever virus (YFV) in Brazil has raised serious concerns due to the rapid dissemination of the virus in the southeastern region. To better understand YFV genetic diversity and dynamics during the recent outbreak in southeastern Brazil, we generated 18 complete and nearly complete genomes from the peak of the epidemic curve from nonhuman primates (NHPs) and human infected cases across the Espírito Santo and Rio de Janeiro states. Genomic sequencing of 18 YFV genomes revealed the estimated timing, source, and likely routes of yellow fever virus transmission and dispersion during one of the largest outbreaks ever registered in Brazil. We showed that during the recent epidemic, YFV was reintroduced from Minas Gerais to the Espírito Santo and Rio de Janeiro states multiple times between 2016 and 2019. The analysis of data from portable sequencing could identify the corridor of spread of YFV. These findings reinforce the idea that continued genomic surveillance strategies can provide information on virus genetic diversity and transmission dynamics that might assist in understanding arbovirus epidemics. IMPORTANCE Arbovirus infections in Brazil, including yellow fever, dengue, zika, and chikungunya, result in considerable morbidity and mortality and are pressing public health concerns. However, our understanding of these outbreaks is hampered by the limited availability of genomic data. In this study, we investigated the genetic diversity and spatial distribution of YFV during the current outbreak by analyzing genomic data from areas in southeastern Brazil not covered by other previous studies. To gain insights into the routes of YFV introduction and dispersion, we tracked the virus by sequencing YFV genomes sampled from nonhuman primates and infected patients from the southeastern region. Our study provides an understanding of how YFV initiates transmission in new Brazilian regions and illustrates that genomics in the field can augment traditional approaches to infectious disease surveillance and control.

scholarly journals Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil

2002 ◽  
Vol 27 (4) ◽  
pp. 372-377 ◽  
Author(s):  
LUCIANA P. AMBROZEVICIUS ◽  
RENATA F. CALEGARIO ◽  
ELIZABETH P.B. FONTES ◽  
MURILO G. DE CARVALHO ◽  
F. MURILO ZERBINI
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Rio De Janeiro ◽  
Minas Gerais ◽  
Southeastern Brazil ◽  
Espírito Santo ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Espirito Santo ◽  
High Degree

The genetic diversity of begomovirus isolates from tomato (Lycopersicon esculentum) fields in the Southeastern region of Brazil was analyzed by direct sequencing of PCR fragments amplified by using universal oligonucleotides for the begomovirus DNA-A, and subsequent computer-aided phylogenetic analysis. Samples of tomato plants and associated weeds showing typical symptoms of virus infection were collected at seven locations in the states of Minas Gerais, Espírito Santo and Rio de Janeiro. A total of 137 out of 369 samples were infected with a begomovirus based on PCR analysis. Phylogenetic analysis indicated a high degree of genetic diversity among begomoviruses infecting tomatoes in the sampled area. One species (Tomato chlorotic mottle virus, TCMV) occurs predominantly in Minas Gerais, whereas in Rio de Janeiro and Espírito Santo a distinct species, not yet fully characterized, predominates. Phylogenetic analysis further indicates the presence of an additional four possible new species. This high degree of genetic diversity suggests a recent transfer of indigenous begomovirus from wild hosts into tomatoes. The close phylogenetic relationship verified between begomovirus infecting tomato and associated weeds favors this hypothesis.

scholarly journals Isolation and characterization of ten new microsatellite markers in Machaerium villosum Vogel (Fabaceae)

Hoehnea ◽  
2014 ◽  
Vol 41 (1) ◽  
pp. 77-80
Author(s):  
João Giudice-Neto ◽  
Rafael Flora Ramos ◽  
Evandro Marsola de Moraes ◽  
Márcio José da Silva ◽  
Vera Nisaka Solferini
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Southeastern Brazil ◽  
Atlantic Rain Forest ◽  
High Genetic Diversity ◽  
Isolation And Characterization ◽  
Minas Gerais State ◽  
The Mean ◽  
Significant Linkage

Machaerium villosum is an important tree species from Southeastern Brazil. We report hereby 10 new microsatellite markers to investigate the structure and genetic diversity of this species. Ninety-seven alleles were detected in 60 specimens from a natural population in Minas Gerais State. High genetic diversity has been found. The mean observed and expected heterozygosities were 0.771 and 0.802, respectively. One locus showed significant Hardy-Weinberg departure and five loci combinations showed significant linkage disequilibrium. These 10 new microsatellite loci will be used to evaluate the genetic diversity of this species in order to understand the fragmentation effects of the Brazilian Atlantic Rain Forest.

scholarly journals Morphological and genetic diversity in Callithrix hybrids in an anthropogenic area in southeastern Brazil (Primates: Cebidae: Callitrichinae)

2017 ◽  
Vol 34 ◽  
pp. 1-9 ◽  
Author(s):  
Adrielle M. Cezar ◽  
Leila M. Pessoa ◽  
Cibele R. Bonvicino
Keyword(s):  
Network Analysis ◽  
Rio De Janeiro ◽  
Phylogenetic Analyses ◽  
Morphological Diversity ◽  
Metropolitan Region ◽  
Southeastern Brazil ◽  
High Similarity ◽  
Saguinus Mystax ◽  
Geographic Structure ◽  
Anthropogenic Area

Two species of Callithrix, C. jacchus (Linnaeus, 1758) and C. penicillata (É. Geoffroy, 1812), are considered invasive in Rio de Janeiro. This study determined the genetic and morphological diversity and verified the species involved in the hybridization of 10 individuals from the municipalities of Silva Jardim (N = 9) and Rio das Ostras (N = 1). We compared the external morphology and skull of C. jacchus (N = 15) and C. penicillata (N = 14) specimens deposited in the collection of the National Museum of Rio de Janeiro (MN- UFRJ). Phylogenetic (maximum likelihood and Bayesian inference) and phylogeographical analyses (network analysis) were performed based on cytochrome b sequences. These analyses included hybrids from the metropolitan region of Rio de Janeiro (N = 3), C. penicillata (N = 2), C. jacchus (N = 2), C. geoffroyi (N = 2), C. kuhlii (N = 2), C. aurita (N = 1), and as outgroups, Mico emiliae (N = 1) and Saguinus mystax (N = 1). The pelage and skull characters of most hybrids were more closely related to C. jacchus. Skull morphometric analysis revealed an intermediate state for the hybrids. Phylogenetic analyses revealed a high similarity between the hybrids and C. penicillata. Six haplotypes of hybrids were identified. Network analysis including them and C. penicillata recovered the topology generated by phylogenetic analysis. The results corroborate that C. jacchus and C. penicillata participate in the hybridization process. There was no geographic structure between hybrids from the coastal lowlands and from the metropolitan region of Rio de Janeiro.

Genetic diversity based on microsatellite markers in five Nile tilapia strains

2009 ◽  
Vol 17 (1) ◽  
pp. 82 ◽  
Author(s):  
Liang Hongwei ◽  
Li Zhong ◽  
Luo Xiangzhong ◽  
Wang Changzhong ◽  
Hu Guangfu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Nile Tilapia

scholarly journals Population structure and genetic diversity of non-native aoudad populations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sunčica Stipoljev ◽  
Toni Safner ◽  
Pavao Gančević ◽  
Ana Galov ◽  
Tina Stuhne ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Control Region ◽  
Effective Population ◽  
The Czech Republic ◽  
Mitochondrial Dna Control Region ◽  
Mountain Ranges ◽  
Spanish Populations ◽  
Microsatellite Alleles ◽  
Shared Alleles ◽  
Number Of Individuals

AbstractThe aoudad (Ammotragus lervia Pallas 1777) is an ungulate species, native to the mountain ranges of North Africa. In the second half of the twentieth century, it was successfully introduced in some European countries, mainly for hunting purposes, i.e. in Croatia, the Czech Republic, Italy, and Spain. We used neutral genetic markers, the mitochondrial DNA control region sequence and microsatellite loci, to characterize and compare genetic diversity and spatial pattern of genetic structure on different timeframes among all European aoudad populations. Four distinct control region haplotypes found in European aoudad populations indicate that the aoudad has been introduced in Europe from multiple genetic sources, with the population in the Sierra Espuña as the only population in which more than one haplotype was detected. The number of detected microsatellite alleles within all populations (< 3.61) and mean proportion of shared alleles within all analysed populations (< 0.55) indicates relatively low genetic variability, as expected for new populations funded by a small number of individuals. In STRUCTURE results with K = 2–4, Croatian and Czech populations cluster in the same genetic cluster, indicating joined origin. Among three populations from Spain, Almeria population shows as genetically distinct from others in results, while other Spanish populations diverge at K = 4. Maintenance of genetic diversity should be included in the management of populations to sustain their viability, specially for small Czech population with high proportion of shared alleles (0.85) and Croatian population that had the smallest estimated effective population size (Ne = 5.4).

scholarly journals Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers

Heredity ◽  
2001 ◽  
Vol 86 (4) ◽  
pp. 469-479 ◽  
Author(s):  
Stéphanie Mariette ◽  
David Chagné ◽  
Céline Lézier ◽  
Patrick Pastuszka ◽  
Annie Raffin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Pinus Pinaster

scholarly journals Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Workia Ahmed ◽  
Tileye Feyissa ◽  
Kassahun Tesfaye ◽  
Sumaira Farrakh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Information Content ◽  
Date Palm ◽  
Polymorphic Information Content ◽  
Phoenix Dactylifera ◽  
Mean Value ◽  
Date Palms ◽  
Expected Heterozygosity

Abstract Background Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustainability and huge socio-economic value in many countries of the world including Ethiopia. Evaluation of genetic diversity across date palms at DNA level is very important for breeding and conservation. The result of this study could help to design for genetic improvement and develop germplasm introduction programmes of date palms mainly in Ethiopia. Results In this study, 124 date palm genotypes were collected, and 10 polymorphic microsatellite markers were used. Among 10 microsatellites, MPdCIR085 and MPdCIR093 loci showed the highest value of observed and expected heterozygosity, maximum number of alleles, and highest polymorphic information content values. A total of 112 number of alleles were found, and the mean number of major allele frequency was 0.26, with numbers ranging from 0.155 (MPdCIR085) to 0.374 (MPdCIR016); effective number of alleles with a mean value of 6.61, private alleles ranged from 0.0 to 0.65; observed heterozygosity ranged from 0.355 to 0.726; expected heterozygosity varied from 0.669 to 0.906, polymorphic information content with a mean value of 0.809; fixation index individuals relative to subpopulations ranged from 0.028 for locus MPdCIR032 to 0.548 for locus MPdCIR025, while subpopulations relative to total population value ranged from − 0.007 (MPdCIR070) to 0.891 (MPdCIR015). All nine accesstions, neighbour-joining clustering analysis, based on dissimilarity coefficient values were grouped into five major categories; in population STRUCTURE analysis at highest K value, three groups were formed, whereas DAPC separated date palm genotypes into eight clusters using the first two linear discriminants. Principal coordinate analysis was explained, with a 17.33% total of variation in all populations. Generally, the result of this study revealed the presence of allele variations and high heterozygosity (> 0.7) in date palm genotypes. Conclusions Microsatellites (SSR) are one of the most preferable molecular markers for the study of genetic diversity and population structure of plants. In this study, we found the presence of genetic variations of date palm genotypes in Ethiopia; therefore, these genetic variations of date palms is important for crop improvement and conservation programmes; also, it will be used as sources of information to national and international genbanks.

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