Evaluation of Bacterial Profile and Drug Resistance Patterns of Blood Culture Isolates in Amir Al-Momenin Hospital of Gerash, Iran

Mycobacterium tuberculosis is a highly studied pathogen due to public health importance. Despite this, problems like early drug resistance, diagnostics and treatment success prediction are still not fully resolved. Here, we analyze the incidence of point mutations widely used for drug resistance detection in laboratory practice and conduct comparative analysis of whole-genome sequence (WGS) for clinical M. tuberculosis strains collected from patients with pulmonary tuberculosis (PTB) and extra-pulmonary tuberculosis (XPTB) localization. A total of 72 pulmonary and 73 extrapulmonary microbiologically characterized M. tuberculosis isolates were collected from patients from 2007 to 2014 in Russia. Genomic DNA was used for WGS and obtained data allowed identifying major mutations known to be associated with drug resistance to first-line and second-line antituberculous drugs. In some cases previously described mutations were not identified. Using genome-based phylogenetic analysis we identified M. tuberculosis substrains associated with distinctions in the occurrence in PTB vs. XPTB cases. Phylogenetic analyses did reveal M. tuberculosis genetic substrains associated with TB localization. XPTB was associated with Beijing sublineages Central Asia (Beijing CAO), Central Asia Clade A (Beijing A) and 4.8 groups, while PTB localization was associated with group LAM (4.3). Further, the XPTB strain in some cases showed elevated drug resistance patterns relative to PTB isolates. HIV was significantly associated with the development of XPTB in the Beijing B0/W148 group and among unclustered Beijing isolates.

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Drug resistance patterns in new and previously treated patients with tuberculosis presenting to a tertiary care center in southern India

BMC Infectious Diseases ◽

10.1186/1471-2334-14-s3-o3 ◽

2014 ◽

Vol 14 (Suppl 3) ◽

pp. O3 ◽

Cited By ~ 1

Author(s):

Anto J Udaykumar ◽

Jayashree R ◽

Baijayanti Mishra ◽

Rosario Vivek ◽

John Kenneth

Keyword(s):

Drug Resistance ◽

Care Center ◽

Tertiary Care ◽

Southern India ◽

Tertiary Care Center ◽

Resistance Patterns ◽

Previously Treated Patients ◽

Previously Treated

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Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan

BMC Infectious Diseases ◽

10.1186/1471-2334-8-171 ◽

2008 ◽

Vol 8 (1) ◽

Cited By ~ 46

Author(s):

Mahnaz Tanveer ◽

Zahra Hasan ◽

Amna R Siddiqui ◽

Asho Ali ◽

Akbar Kanji ◽

...

Keyword(s):

Drug Resistance ◽

Resistance Patterns

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Changing patterns and widening of antibiotic resistance inShigellaspp. over a decade (2000–2011), Andaman Islands, India

Epidemiology and Infection ◽

10.1017/s0950268814000958 ◽

2014 ◽

Vol 143 (3) ◽

pp. 470-477 ◽

Cited By ~ 12

Author(s):

D. BHATTACHARYA ◽

H. BHATTACHARYA ◽

D. S. SAYI ◽

A. P. BHARADWAJ ◽

M. SINGHANIA ◽

...

Keyword(s):

Drug Resistance ◽

Antibiotic Resistance ◽

Wide Spectrum ◽

Resistance Pattern ◽

Third Generation ◽

Isolation And Identification ◽

Antimicrobial Drugs ◽

Drug Resistance Pattern ◽

Resistance Patterns ◽

Third Generation Cephalosporins

SUMMARYThis study is a part of the surveillance study on childhood diarrhoea in the Andaman and Nicobar Islands; here we report the drug resistance pattern of recent isolates ofShigellaspp. (2006–2011) obtained as part of that study and compare it with that ofShigellaisolates obtained earlier during 2000–2005. During 2006–2011, stool samples from paediatric diarrhoea patients were collected and processed for isolation and identification ofShigellaspp. Susceptibility to 22 antimicrobial drugs was tested and minimum inhibitory concentrations were determined for third-generation cephalosporins, quinolones, amoxicillin-clavulanic acid combinations and gentamicin. A wide spectrum of antibiotic resistance was observed in theShigellastrains obtained during 2006–2011. The proportions of resistant strains showed an increase from 2000–2005 to 2006–2011 in 20/22 antibiotics tested. The number of drug resistance patterns increased from 13 in 2000–2005 to 43 in 2006–2011. Resistance to newer generation fluoroquinolones, third-generation cephalosporins and augmentin, which was not observed during 2000–2005, appeared during 2006–2011. The frequency of resistance inShigellaisolates has increased substantially between 2000–2006 and 2006–2011, with a wide spectrum of resistance. At present, the option for antimicrobial therapy in shigellosis in Andaman is limited to a small number of drugs.

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Characterization and Drug Resistance Patterns of Ewing's Sarcoma Family Tumor Cell Lines

PLoS ONE ◽

10.1371/journal.pone.0080060 ◽

2013 ◽

Vol 8 (12) ◽

pp. e80060 ◽

Cited By ~ 26

Author(s):

William A. May ◽

Rita S. Grigoryan ◽

Nino Keshelava ◽

Daniel J. Cabral ◽

Laura L. Christensen ◽

...

Keyword(s):

Drug Resistance ◽

Tumor Cell ◽

Cell Lines ◽

Ewing’S Sarcoma ◽

Ewing's Sarcoma ◽

Tumor Cell Lines ◽

Resistance Patterns

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The frequency of bacterial contamination and diversity of drug resistance patterns in devices and staff of endoscopy and colonoscopy units

Medical Journal of the Islamic Republic of Iran ◽

10.14196/mjiri.31.104 ◽

2017 ◽

Vol 31 (1) ◽

pp. 705-708 ◽

Cited By ~ 1

Author(s):

Parisa Torabi ◽

Masoumeh Azimirad ◽

Zahra Hasani ◽

Leili Afrisham ◽

Masoud Alebouyeh ◽

...

Keyword(s):

Drug Resistance ◽

Bacterial Contamination ◽

Resistance Patterns

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Drug resistance patterns of Gram-negative bacteria isolated from food and non-sterile medicines in hospital environments

Journal of Hospital Infection ◽

10.1016/0195-6701(81)90068-2 ◽

1981 ◽

Vol 2 ◽

pp. 355-361

Author(s):

M.D. Simon-Pujol ◽

M. Matabosch ◽

M.J. Espuny-Tomas ◽

A.M. Marques ◽

F. Congregado

Keyword(s):

Drug Resistance ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Hospital Environments ◽

Resistance Patterns

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Drug resistance and plasmid profile of shigellae in Taiwan

Epidemiology and Infection ◽

10.1017/s0950268800049530 ◽

1992 ◽

Vol 108 (1) ◽

pp. 87-97 ◽

Cited By ~ 7

Author(s):

S.-R. Lin ◽

S.-F. Chang

Keyword(s):

Drug Resistance ◽

Antimicrobial Agents ◽

Plasmid Profile ◽

Resistance Pattern ◽

Multiple Resistance ◽

College Hospital ◽

Medical College ◽

Transmissible Plasmid ◽

Resistance Patterns ◽

Species Specific

SUMMARYOne hundred and twenty-eight shigella strains isolated from newborn and infant human faecal specimens at Kaohsiung Medical College Hospital in Taiwan were serogrouped, serotyped and examined for drug-resistance patterns and for the presence of plasmids. Forty-seven pre cent of the isolates were found to belong to theShigella sonneiserogroup, 41%to theS.flexnerigroup,9%to theS.boydiigroup and 3%to theS.dysenteriaegroup.The serotype with the greatest number of strains wasS.sonneiI. (29 %) followed byS. flexneri1 (27%). Each strain was tested for resistance to 11 antimicrobial agents. Eighty-eight per cent of the strains were resistant to tetracycline, 87% to chloramphenicol, 84% to streptomycin, 52% to ampicillin, 25% to nalidixic acid, 29% to kanamycin, 11 % to cephalothin, 11% to neomycin, 10% to cotrimoxazole, 1% to amikacin and none to gentamicin. The most prevalent resistance pattern was ApCmSmTc (28%). Clinical isolates demonstrating multiple resistance were found to harbour a large transmissible plasmid of 45–75 MDa while isolates without multiple resistance did not. Two large virulence plasmids of 123 and 110 MDa were found in 12 strains ofS. flexneriand 4 strains ofS. sonneiphase I. Small plasmids of 4·5, 4·2, 3·5, 2·8, 2·5. 2·0 and 1·5 MDa were also present in all strains. These small plasmids were species specific and can be used as marker plasmids to identify species.

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