scholarly journals Detection of Mulatexin in Local Mulberry Plants (Morus spp.) from Bogor, West Java, Indonesia

2021 ◽  
Vol 28 (3) ◽  
pp. 193
Author(s):  
Yasinta Ratna Esti Wulandari ◽  
Yogiara Yogiara ◽  
Isabel Livena Young
Keyword(s):  
Gene Family ◽  
Bombyx Mori ◽  
Morus Alba ◽  
Insect Herbivores ◽  
Genomic Rna ◽  
West Java ◽  
Plant Organs ◽  
Morus Spp

The mlx56 gene family is found exclusively in the mulberry (Morus spp.) plant, and its role is to defend against insect herbivores, yet it had never been discovered from Indonesia, which has been cultivating mulberry silkworm's (Bombyx mori) feed in order to produce silks. This research focused on studying the expression of mlx56 gene family in local mulberry plants from Bogor, West Java, using different plant organs which were leaf, stem, and root. Each sample was collected from four species of mulberry; Morus alba var. kanva-2, M. alba var. multicaulis, M. bombycis var. lembang, and M. cathayana. Genomic RNA samples were collected and synthesized into cDNA, amplification was done using eight primers of the mlx56 gene family and the products were sequenced. Sequences were then analyzed by BLAST. Results showed diverse mlx56 gene family expression among species of mulberry and plant organs. The mlx56-2, mlx56-4, and mlx56-7, were mostly expressed in all species and organs. The gene mlx56-3, which was not discovered in the previous study, was expressed in M. cathayana leaf and root also in the roots of other species. It was identified as chitinase-like protein LA along with mlx56-5, while other genes were identified as mlx56 based on BLAST.

scholarly journals Efeito do ácido beta indolacético e de cloreto de cálcio no enraizamento de estacas da amoreira (Morus alba, L., var. Catânia 1) em estufim, plantadas na posição invertida

1976 ◽  
Vol 33 (0) ◽  
pp. 309-313
Author(s):  
Antonio Castilho Rúbia ◽  
José Renato Sarruge ◽  
Eduardo Abramides
Keyword(s):  
Bombyx Mori ◽  
Morus Alba ◽  
Bombyx Mori L

Os autores apresentam neste trabalho os resultados de experimento de enraizamento de estacas de amoreira (Morus alba L., var. Catânia 1) com o emprego de hormônio vegetal sintético, ácido Beta indolacético (100 ppm) e soluções de cloreto de cálcio (2,5 5,00 10,00 iônios Ca++/1.000 ml) . Aquela variedade, uma das mais produtiva em folhas que por sua vez se apresentam mais ricas em elementos nutritivos à alimentação do bicho-da-seda (Bombyx mori L.), dificilmente se propaga pela estaquia natural, o que impede seu cultivo no sistema de "cepo". Depois das estacas da amoreira (Morus alba L., var. Catânia 1) terem sido preparadas e tratadas durante 24 horas em vasilhames de polietileno, foram no dia 24 de outubro de 1973, plantadas na posição invertida em substrato ,areia grossa lavada) contido em estufim. A retirada das estacas e consequentemente a conclusão do experimento, verificou-se 110 dias após seu plantio.

Codon-pair usage pattern and cluster analysis of the ABC gene family in silkworm, Bombyx mori

Gene Reports ◽  
2018 ◽  
Vol 13 ◽  
pp. 173-179
Author(s):  
Gang Li ◽  
Kaiyue Zhou ◽  
Heying Qian ◽  
Guodong Zhao ◽  
Anying Xu
Keyword(s):  
Cluster Analysis ◽  
Gene Family ◽  
Bombyx Mori ◽  
Usage Pattern ◽  
Codon Pair ◽  
And Cluster Analysis ◽  
Silkworm Bombyx Mori

scholarly journals Caracterização bromatológica de cultivares de amoreira, Morus spp., e determinação dos índices nutricionais de Bombyx mori L. (Lepidoptera: Bombycidae)

2010 ◽  
Vol 39 (4) ◽  
pp. 506-512 ◽  
Author(s):  
Ana M Meneguim ◽  
Cristina Lustri ◽  
Dássia D de Oliveira ◽  
Inês F U Yada ◽  
Amarildo Pasini
Keyword(s):  
Bombyx Mori ◽  
Morus Spp ◽  
Bombyx Mori L

The Transcription Express Characteristics of Several Genes in the Process of Bombyx mori Ovarian Carcinoma

2013 ◽  
Vol 796 ◽  
pp. 39-42
Author(s):  
Xie Yi ◽  
Li Bing
Keyword(s):  
Ovarian Carcinoma ◽  
Gene Family ◽  
Bombyx Mori ◽  
Cell Lines ◽  
P53 Gene ◽  
Ovary Cell ◽  
Ovary Cells ◽  
Bmn Cell ◽  
Cyclin Gene ◽  
Materials Used

Bombyx mori cell line (BmN) comes fromBombyx moriovary cell subculture. In order to study the change of several genes transcription in the process ofBombyx moriovary cells primary culture and subculture, we usedBombyx moriovary organizations and BmN cell lines as research materials, used Real Time fluorescent quantitative RT-PCR to detect cyclin gene family (CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1), p53 and Telomerase genes transcription level in the ovary and BmN cell lines, and took Actin3 gene as reference to dispose the results. The results showed that in theBombyx moriBmN cell lines the expression of CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1 and Telomerase genes were higher than those in the ovary. The expression of CyclinB in the BmN was more then 3.8 which was 76 times higher than that in the ovary; The expression of p53 gene in the BmN cell was lower than that in the ovary; The expression of Telomerase gene in the BmN cell was higher than that in the ovary. The results accumulated a reliable data for further study on the the role of cyclin gene family, p53 gene, and Telomerase gene in the process ofBombyx moriovarian carcinoma.

Genome-wide analysis of the ATP-binding cassette (ABC) transporter gene family in the silkworm, Bombyx mori

2012 ◽  
Vol 39 (7) ◽  
pp. 7281-7291 ◽  
Author(s):  
Xiaodong Xie ◽  
Tingcai Cheng ◽  
Genhong Wang ◽  
Jun Duan ◽  
Weihuan Niu ◽  
...  
Keyword(s):  
Gene Family ◽  
Bombyx Mori ◽  
Abc Transporter ◽  
Atp Binding ◽  
Transporter Gene ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Silkworm Bombyx Mori ◽  
Atp Binding Cassette

The ionotropic γ-aminobutyric acid receptor gene family of the silkworm, Bombyx mori

Genome ◽  
2010 ◽  
Vol 53 (9) ◽  
pp. 688-697 ◽  
Author(s):  
Lin-Lin Yu ◽  
Ying-Jun Cui ◽  
Guo-Jun Lang ◽  
Ming-Yan Zhang ◽  
Chuan-Xi Zhang
Keyword(s):  
Gene Family ◽  
Bombyx Mori ◽  
Gaba Receptors ◽  
Receptor Gene ◽  
Aminobutyric Acid ◽  
Inhibitory Neurotransmitter ◽  
Duplication Events ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Silkworm Bombyx Mori

γ-Aminobutyric acid (GABA) is a very important inhibitory neurotransmitter in both vertebrate and invertebrate nervous systems. GABA receptors (GABARs) are known to be the molecular targets of a class of insecticides. Members of the GABAR gene family of the silkworm, Bombyx mori , a model insect of Lepidoptera, have been identified and characterized in this study. All putative silkworm GABAR cDNAs were cloned using the reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Bombyx mori appears to have the largest insect GABAR gene family known to date, including three RDL, one LCCH3, and one GRD subunit. The silkworm RDL1 gene has RNA-editing sites, and the RDL1 and RDL3 genes possess alternative splicing. These mRNA modifications enhance the diversity of the silkworm’s GABAR gene family. In addition, truncated transcripts were found for the RDL1 and LCCH3 genes. In particular, the three RDL subunits may have arisen from two duplication events.

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