scholarly journals Isolation of Ganoderma lucidum (Curtis) P. Karst. From the Wild in Lagos through Tissue Culture Techniques and Cultivation on Sawdust of Six Nigerian Hardwoods

2021 ◽  
Vol 25 (7) ◽  
pp. 1329-1335
Author(s):  
E.M. Adongbede ◽  
A.F. Atoyebi
Keyword(s):  
Tissue Culture ◽  
Wheat Bran ◽  
Ganoderma Lucidum ◽  
Cold Treatment ◽  
Biological Efficiency ◽  
Malt Extract ◽  
Potential Growth ◽  
Culture Techniques ◽  
Commercial Cultivation ◽  
Nauclea Diderrichii

Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used for cultivation in Asia, America and Europe are not available in Nigeria. The present study investigated indigenous hardwoods and their supplementation with rice and wheat bran as substrates for its cultivation. Six hardwoods (Mansonia altissima (A Chev.) A Chev., Avecennia germinans (L.) L, Lophira alata Banks ex Gaertn., Triplochiton scleoxylon K. Schum, Uapaca guineensis Mull. Arg, Nauclea diderrichii (De Wild. & Th. Dur.) Merrill) were investigated as potential growth substrates and potentially improve biological efficiency. The mushroom was collected from the wild and identification confirmed by amplifying the ribosomal DNA-ITS fragment with ITS1 and ITS4 primers. Tissue culture of the mushroom collected from the wild was initiated successfully with modified malt extract agar and grain spawn developed from it. The substrates were incubated after inoculation with grain spawn at room temperature for 30-60 days. Substrates were given cold treatment for 7days to induce fructification which was achieved with 9-10h/day regime and daily watering. There was full mycelial ramification of all substrates by the mushroom. Fruit bodies were harvested from all substrates but Lophira alata. The highest yield (308.76±5.81g/kg) was recorded with Mansonia altissima (Biological Efficiency-31.42±4.55%) and the least yield (31.45±5.44g/kg) was recorded in Nauclea diderrichii (B.E- 5.25±0.58%). The substrates with wheat bran performed significantly better than rice bran supplemented substrates. Commercial cultivation of native G. lucidum in Nigeria is possible with local agricultural wastes.

scholarly journals Isolation of Ganoderma lucidum (Curtis) P. Karst. From the Wild in Lagos through Tissue Culture Techniques and Cultivation on Sawdust of Six Nigerian Hardwoods

2021 ◽  
Vol 25 (8) ◽  
pp. 1497-1503
Author(s):  
E.M. Adongbede ◽  
A.F. Atoyebi
Keyword(s):  
Tissue Culture ◽  
Wheat Bran ◽  
Ganoderma Lucidum ◽  
Cold Treatment ◽  
Biological Efficiency ◽  
Malt Extract ◽  
Potential Growth ◽  
Culture Techniques ◽  
Commercial Cultivation ◽  
Nauclea Diderrichii

Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used for cultivation in Asia, America and Europe are not available in Nigeria. The present study investigated indigenous hardwoods and their supplementation with rice and wheat bran as substrates for its cultivation. Six hardwoods (Mansonia altissima (A Chev.) A Chev., Avecennia germinans (L.) L, Lophira alata Banks ex Gaertn., Triplochiton scleoxylon K. Schum, Uapaca guineensis Mull. Arg, Nauclea diderrichii (De Wild. & Th. Dur.) Merrill) were investigated as potential growth substrates and potentially improve biological efficiency. The mushroom was collected from the wild and identification confirmed by amplifying the ribosomal DNA-ITS fragment with ITS1 and ITS4 primers. Tissue culture of the mushroom collected from the wild was initiated successfully with modified malt extract agar and grain spawn developed from it. The substrates were incubated after inoculation with grain spawn at room temperature for 30-60 days. Substrates were given cold treatment for 7days to induce fructification which was achieved with 9-10h/day regime and daily watering. There was full mycelial ramification of all substrates by the mushroom. Fruit bodies were harvested from all substrates but Lophira alata. The highest yield (308.76±5.81g/kg) was recorded with Mansonia altissima (Biological Efficiency-31.42±4.55%) and the least yield (31.45±5.44g/kg) was recorded in Nauclea diderrichii (B.E- 5.25±0.58%). The substrates with wheat bran performed significantly better than rice bran supplemented substrates. Commercial cultivation of native G. lucidum in Nigeria is possible with local agricultural wastes.

scholarly journals Artificial cultivation of a novel wild strain of Lepista sordida (Sowerby) Pat from south west China

2021 ◽  
pp. 813-818
Author(s):  
De Xu ◽  
Hui Zhao ◽  
Biao Li ◽  
Chuanqi Sun ◽  
Zhide Wang ◽  
...  
Keyword(s):  
Field Experiments ◽  
Wild Strain ◽  
Fruit Body ◽  
Biological Efficiency ◽  
Morphological Description ◽  
Malt Extract ◽  
Soybean Field ◽  
Commercial Cultivation ◽  
Western Area ◽  
Body Production

A novel wild strain of Lepista sordida mushroom was collected from a soybean field in Liujiaba at Dazhou district of south western area of China in 2015.The morphological description and molecular identification were conducted to confirm the species. In the present study, optimization was carried out from mycelial growth and fruit body production. For DAAS-E70, the best growth rate was obtained using yeast malt extract, pH 6.0 and temperature 25°C. The wheat grain mushroom spawn was used for upscaling of the mycelium to be used for fruiting body production. In the field experiments, the average biological efficiency of DAAS-E70 was higher than that of Jilin-1, in which, DAAS-E70 accounting for 41.22% of the average biological efficiency, while the Jilin-1 was 35.02%. The stable biological efficiency of DAAS-E70 in different environments and seasons showed that the DAAS-E70 could be used for its commercial cultivation and could provide a new reference for its artificial domestication. Bangladesh J. Bot. 50(3): 813-818, 2021 (September) Special

Cytogenetic Analysis of 39 Prostate Carcinomas and Evaluation of Short Term Tissue Culture Techniques

1998 ◽  
Vol 101 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Josée J König ◽  
Wilma Teubel ◽  
Eric Kamst ◽  
Johannes C Romijn ◽  
Fritz H Schröder ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cytogenetic Analysis ◽  
Short Term ◽  
Culture Techniques ◽  
Prostate Carcinomas ◽  
Tissue Culture Techniques

DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

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