scholarly journals Studies on the Thermal Stability of Peroxidase from Leaf of Oil Palm (Elaeis guineensis)

2021 ◽  
Vol 25 (7) ◽  
pp. 1163-1166
Author(s):  
J.N. Ozioko ◽  
B.O. Ezema ◽  
K.O. Omeje ◽  
S.O.O. Eze

Peroxidase was extracted from leaves of oil palm tree with 0.01M phosphate buffer pH 7.0. It was partially purified using 70% ammonium sulphate ((NH4)2SO4) precipitation. This resulted in peroxidase with activity of (26U/ml) and specific activity of 35.8U/mg. Effect of heat on the activity of peroxidase was studied at temperature of 323-363°K. After gel filtration on sephadex G100, the peroxidase activity increased to 27U/ml, with specific activity of 55U/mg .The overall purification fold was 4 with 51.9% enzyme recovery. The peroxidase partially purified from leaves of oil palm tree showed pH and temperature optima of 5.0 and 50°C respectively. High pH and temperature stabilities of pH 5.0 to pH 9.0 and 50°C to 70°C were obtained respectively. Also, the activation energy (Ea) of the reaction was - 21.616kj/mol. The free energy changes (ΔG) were 96008.64, 96315.59, 97901.63, 94132.33 and 97146.75kj/mol at 323,333,343,353 and 363°K respectively. It was observed that the D-values were decreasing with increasing temperature with a Z-value of 0.044. The enthalpy results suggest that the reaction was exothermic, non-spontaneous and reversible.

2002 ◽  
Vol 269 (10) ◽  
pp. 2584-2590 ◽  
Author(s):  
Anabel Rodríguez ◽  
David G. Pina ◽  
Belén Yélamos ◽  
John J. Castillo León ◽  
Galina G. Zhadan ◽  
...  

2021 ◽  
Vol 9 (1) ◽  
pp. 32-35
Author(s):  
Lal awmpuia ◽  
◽  
H. Lalruatsanga ◽  

A survey of plant species inhabiting oil palm trees was conducted in Zawlpui area of Serchhip district, Mizoram. The study area is a tropical potent agriculture zone, wherein small-scale business of Elaeis guineensis plantation is carried out by several farmers mainly within the gentle sloppy terrain. Oil palm with a rough bark harbors immense inhabitation by a variety plants, that rooted mostly on the debris at leaf base. Species diversity on the plant stem supposedly encourage insects and termites to establish herewith, thus causing harming to the tree. The sample stands within 400 m–800 m altitude were picked randomly. A total of 50 palm tree were accounted and all associated plants on the stem above 30 cm from the ground are all recorded. Species that cannot be identified on the site were pressed and observed at Botany Research lab, Pachhunga University College. The survey documented 38 vascular plant species which include 4 epiphytes and 1 non-vascular species of lichen, 1 bryophyte and 4 fungal species at that time. Invasive Peperomia pellucida and epiphytic pteridophytes Nephrolepis biserrata was found in most of the stand sample; however, Peperomia population decreases with the increasing elevation. Dynamics of inhabitant species diversity also correlate to location of tree. The study also established that diversity of inhabiting species was comparatively high on parts of the stem facing sunlight.


2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Simone Almeida Pena ◽  
Ana Cristina Mendes-Oliveira

Abstract: In this study we described the diet of Hylaeamys megacephalus (G. Fisher, 1814) and investigated the degree of individual variation in the diet of this species among the Amazon Forest and the oil palm plantation. We analyzed the stomach contents of 36 individuals, of whom 11 were collected in the forest and 25 captured in the palm oil palm plantation. The H. megacephalus diet consisted of 18 food items, of which 12 were animal composition and eight were vegetable composition. The niche amplitude of the species was narrower in the forest area (Baforest = 0.013) compared to the palm tree plantation area (Bapalm = 0.478). This shows that individuals have greater niche overlap in forest areas, while in the plantation areas the animals expand their food niche. In addition, the values of the mean of the individual diet in relation to the diet of the entire population were lower in the palm oil palm plantation environment (ISpalm = 0.164) than in the Forest environment (ISforest = 0.357), indicating a high specialization in the palm oil plantation. These results indicate a population mechanism to reduce intraspecific competition in response to scarce resources.


2020 ◽  
Vol 14 (1) ◽  
pp. 94-108 ◽  
Author(s):  
Emmanuel Owoichoechi Momoh ◽  
Adelaja Israel Osofero

AbstractFibers obtained from different parts of the oil palm tree (Elaeis guineensis) have been under investigation for possible use in construction. Studies have been carried out investigating the engineering properties and possible applications of these fibers. However, the experimental methods employed and the values of mechanical and physical properties recorded by various authors are inconsistent. It has therefore become necessary to organize information which would be useful in the design of oil palm fiber cement composites and help researchers and engineers make informed decisions in further research and application. This review provides information about fibers from different parts of the oil palm, their properties, enhancement techniques, current and potential application in cement composites.


1983 ◽  
Vol 50 (2) ◽  
pp. 171-184 ◽  
Author(s):  
Leszek Stepaniak ◽  
Patrick F. Fox

SummaryA metalloproteinase, isolated from a shaken milk culture ofPseudomonas fluorescensAFT 36 by chromatography in DEAE and CM-cellulose and Sephadex G-150, was very unstable in 0·1 m-phosphate buffer, pH6·6, being completely denatured above 70 °C in 1 min. It was also unstable in a Ca-containing buffer (synthetic milk salts, SMS) between 50 and 60 °C (minimum at 55 °C), but stability was very high above 80 °C in this buffer. D-values were determined at 10 °C intervals in the range 70–150 °C in SMS from which a Z value of 31·9 °C and anEaof 8·82 × 104J mol-1were calculated; the half-life at 150 °C was 9 s.Instability at 55 °C was due to autolysis as evidenced by gel electrophoresis, gel filtration and increase in 2,4,6-trinitrobenzenesulphonic acid-reactive amino groups. The extent of inactivation experienced at 80 °C was inversely related to the rate of heating to 80 °C,i.e.length of time spent in the neighbourhood of 55 °C. Addition of increasing concentrations of caseinate substrate reduced inactivation of the enzyme at 55 °C, presumably due to substrate binding. Attempts to stabilize the enzyme at 55 °C by addition of EDTA or by adjusting the reaction pH to 4·2, at which the enzyme has little proteolytic activity, were unsuccessful, although autolysis was prevented.Unlike the proteinase fromPs. fluorescensMC 60, AFT 36 proteinase did not inactivate itself on cooling to 55 °C from 80, 100 or 150 °C, but did regain autolytic activity on cooling to below 50 °C to an extent dependent on the duration of holding at the lower temperature. It is suggested that on heating to ∼ 55 °C, a conformational change occurs which renders the enzyme susceptible to proteolysis by still active enzyme; at higher temperatures the enzyme, although susceptible to autolysis, is inactive; an active conformation is restored on cooling to below 50 °C.


1986 ◽  
Vol 64 (1) ◽  
pp. 214-216 ◽  
Author(s):  
O. O. Otusanya ◽  
A. C. Adebona

During a 1-year cycle the acidic, ethyl acetate soluble gibberellinlike activity in the phloem sap of the emergent male inflorescence of the oil palm tree (Elaesis guineensis Jacq.) was assayed with barley half-seeds after separation on paper chromatography. Gibberellinlike substances which cochromatograph with gibberellic acid 3 were translocated to the male emerging inflorescence stalk. Their quantity was maximal (45 μg L−1 of sap) in late November, and minimal (1.5 μg L−1 of sap) during March to August.


2021 ◽  
Vol 14 (10) ◽  
pp. 961
Author(s):  
Wai-Kit Tow ◽  
Asly Poh-Tze Goh ◽  
Usha Sundralingam ◽  
Uma Devi Palanisamy ◽  
Yasodha Sivasothy

The oil palm tree (Elaeis guineensis Jacq.) originates from West and Central Africa, and it is cultivated in Malaysia for its oil-producing fruits. Malaysia is the world’s second largest palm oil producer and the world’s largest exporter to date. Consequently, the Malaysian oil palm industry constantly generates a huge amount of biomass with the major contributor being the leaves. A large percentage of these leaves remain underutilized, making them a promising source of raw materials that can be converted into value-added products. The present review summarizes and discusses the flavonoid composition, total phenolic and flavonoid content, and the in vitro and in vivo pharmacological properties exhibited by the extracts of the leaves of E. guineensis. The purpose of this systematic review is to highlight the potential of valorizing the leaf extracts of the oil palm tree as pharmaceutical and cosmeceutical agents.


2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Robert P. Weinberg ◽  
Vera V. Koledova ◽  
Hyeari Shin ◽  
Jennifer H. Park ◽  
Yew Ai Tan ◽  
...  

Alzheimer’s disease is a severe neurodegenerative disease characterized by the aggregation of amyloid-βpeptide (Aβ) into toxic oligomers which activate microglia and astrocytes causing acute neuroinflammation. Multiple studies show that the soluble oligomers of Aβ42 are neurotoxic and proinflammatory, whereas the monomers and insoluble fibrils are relatively nontoxic. We show that Aβ42 aggregation is inhibitedin vitroby oil palm phenolics (OPP), an aqueous extract from the oil palm tree(Elaeis guineensis). The data shows that OPP inhibits stacking ofβ-pleated sheets, which is essential for oligomerization. We demonstrate the inhibition of Aβ42 aggregation by (1) mass spectrometry; (2) Congo Red dye binding; (3) 2D-IR spectroscopy; (4) dynamic light scattering; (5) transmission electron microscopy; and (6) transgenic yeast rescue assay. In the yeast rescue assay, OPP significantly reduces the cytotoxicity of aggregating neuropeptides in yeast genetically engineered to overexpress these peptides. The data shows that OPP inhibits (1) the aggregation of Aβinto oligomers; (2) stacking ofβ-pleated sheets; and (3) fibrillar growth and coalescence. These inhibitory effects prevent the formation of neurotoxic oligomers and hold potential as a means to reduce neuroinflammation and neuronal death and thereby may play some role in the prevention or treatment of Alzheimer’s disease.


2017 ◽  
Vol 13 (12) ◽  
pp. 214
Author(s):  
Boubacar Camara ◽  
Boubacar Sagna ◽  
Daouda Ngom ◽  
Mamadou Niokane ◽  
Zoe Daba Gomis

Lower Casamance harbors enormous economic potentialities due to the importance of its forest resources. Among the forest species of the area, Elaeis guineensis is one of the most exploited for its many services and products. Despite its importance, the oil palm tree is experiencing real threats linked to a combination of natural and anthropogenic factors. The overall objective of this study is to contribute and evaluate the goods and services provided by the agroforestry parks in Lower Casamance which will lead to an alert to the whole community about the threats to these secular systems. For that purpose, an individual questionnaire was administered to a sample of 146 invidious (33 in Carounate, 57 in Kabiline and 56 in Kaguite) according to their business sector in the palm plantation in each of the sites. Corresponding to 5% poll rate. It should be noted that most of the surveyed population acquires their product through harvesting (72% in Carounate, 70.97% in Kabiline and 70.21 in Kaguite). All parts of the oil palm are exploited by the local population for various purposes. Thus the most important frequencies are the use in construction (100%), the production of palm oil on average (94.87% and the making of brooms on average (92.97%). The sale of the palm products is a source of unshakeable revenue that can provide up to 865000F cfa for palm oil, 675000Fcfa for palm wine and 280000F cfa for brooms. This is why, according to 90.91% of the respondents in Carounate, 37.41% in Kabiline and 45.71%, the exploitation of the oil palm occupies the second place, after agriculture, in their activities. It is therefore all the interest to permanently preserve these secular systems.


2018 ◽  
Vol 46 (1) ◽  
pp. 111 ◽  
Author(s):  
Edi Santosa ◽  
Indra Mario Stefano ◽  
Abdul Gani Gani Tarigan ◽  
Ade Wachjar ◽  
Sofyan Zaman ◽  
...  

<p align="center"><strong>ABSTRACT</strong></p>In a long life cycle of oil palm plantation, sustainable water management is mandatory because irrigation is rarely applied. In order to develop water management for sustainable palm oil production, tree-based water footprint of well-established oil palm plantation was assessed. Field data were collected from February to June 2016 in Dolok Ilir managed by PTPN IV, North Sumatera, Indonesia. Additional data were obtained from interviews on the site and the surrounding estates, reports and references. Results showed that water footprint (WF) for production of fresh fruit bunch (FFB) was 510.69 m3 tonne-1 and crude palm oil (CPO) was 517.79 m3 tonne-1. Green, blue and grey water contributed 94.78%, 0.71% and 4.50% in FFB, and 93.48%, 1.66% and 4.85% in CPO productions, respectively. All green WF was calculated basen on actual value of tree evapotranspiration, therefore, the value was mostly lower than other researchs. Low amount of blue water indicates that the oil palm tree in North Sumatera extracts low amount of ground water. On the other hand, grey water for pollution dilution of fertilizers, pesticides and herbicides were high, i.e., 15.15 m3, 4.77 m3, 3.07 m3 tonne-1 FFB, respectively. It implies that reduction of grey water should be implemented in the near future through precission farming.<br /><br />Keywords: CPO, Elaeis guineensis, precission farming, sustainable production, water footprint<br /><br /><br />


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