scholarly journals MicroRNA-16 inhibits the migration and invasion of glioma cell by targeting Bcl-2 gene

2021 ◽  
Vol 19 (12) ◽  
pp. 2499-2504
Author(s):  
Baochang Luo ◽  
Jing Zhang

Purpose: To investigate the effect of microRNA-16 (miR-16) on glioma cell migration and invasiveness, and the mechanism involved.Methods: MicroRNA-16 mimic or inhibitor was transfected into human glioma (SHG44) cells. Cell migration, invasiveness and morphology were determined using scratch test, Transwell invasion assay, and immunohistochemical staining, respectively. Expressions of bcl-2, MMP-9 and MMP-2, and NF-κB1 proteins were measured using Western blotting.Results: Overexpression of MicroRNA-16 significantly down-regulated MMP-9 protein in SHG44 cells (p < 0.05), but MMP-2 protein expressions in the 2 groups were comparable (p > 0.05). Protein expressions of MMP-9 and NF-κB1 were significantly down-regulated in human glioma positive cells, relative to negative control.Conclusion: MiR-16 overexpression suppresses the migration and invasiveness of SHG44 cells via the regulation of NF-κB1/MMP-9 signaling pathway, and it directly targets bcl-2 gene by inhibiting its protein expression. This finding affords a new target for developing new anti-glioma drugs. Keywords: Bcl-2, Expression, Glioma, MicroRNA-16, NF-κB1signaling pathway

2016 ◽  
Vol 18 (suppl_6) ◽  
pp. vi60-vi60
Author(s):  
Rosa Schneiderman ◽  
Anna Shteingauz ◽  
Moshe Giladi ◽  
Tali Voloshin ◽  
Yaara Porat ◽  
...  

2012 ◽  
Vol 23 (14) ◽  
pp. 2635-2644 ◽  
Author(s):  
Tao Tao ◽  
Chun Cheng ◽  
Yuhong Ji ◽  
Guangfei Xu ◽  
Jianguo Zhang ◽  
...  

The Notch signaling regulator Numblike (Numbl) is expressed in the brain, but little is known regarding its role in the pathophysiology of glial cells. In this paper, we report that Numbl expression was down-regulated in high-grade human glioma tissue samples and glioblastoma cell lines. To investigate the role of Numbl in glioma migration and invasion, we generated human glioma cell lines in which Numbl was either overexpressed or depleted. Overexpression of Numbl suppressed, while elimination of Numbl promoted, the migration and invasion of glioma cells. Numbl inhibited glioma migration and invasion by dampening NF-κB activity. Furthermore, Numbl interacted directly with tumor necrosis factor receptor–associated factor 5 (TRAF5), which signals upstream and is required for the activation of NF-κB, and committed it to proteasomal degradation by promoting K48-linked polyubiquitination of TRAF5. In conclusion, our data suggest that Numbl negative regulates glioma cell migration and invasion by abrogating TRAF5-induced activation of NF-κB.


2013 ◽  
Vol 31 (4) ◽  
pp. 234-241 ◽  
Author(s):  
Zheng-Xiang Han ◽  
Xiao-Xia Wang ◽  
Shang-Nuan Zhang ◽  
Jin-Xia Wu ◽  
He-ya Qian ◽  
...  

Oncogene ◽  
2018 ◽  
Vol 38 (10) ◽  
pp. 1734-1750 ◽  
Author(s):  
J. P. Zepecki ◽  
K. M. Snyder ◽  
M. M. Moreno ◽  
E. Fajardo ◽  
A. Fiser ◽  
...  

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Mitsutoshi Nakada ◽  
Eric M. Anderson ◽  
Tim Demuth ◽  
Satoko Nakada ◽  
Linsey B. Reavie ◽  
...  

2005 ◽  
Vol 144 (8) ◽  
pp. 1032-1036 ◽  
Author(s):  
Angelo Vaccani ◽  
Paola Massi ◽  
Arianna Colombo ◽  
Tiziana Rubino ◽  
Daniela Parolaro

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