scholarly journals Acute toxicity study of the ethanolic extract of Eleutherine bulbosa Urb in Wistar rats

2021 ◽  
pp. 143-147
Author(s):  
Helmina Wati ◽  
Rahmi Muthia ◽  
Kartini ◽  
Finna Setiawan
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Test Study ◽  
Limit Test ◽  
Low Toxicity ◽  
Medicinal Properties

Introduction: Eleutherine bulbosa Urb is a plant species with medicinal properties, including anti-inflammatory, widely relied upon in traditional practices. For this reason, the present research was intended to assess and, thus, ensure the safety of this plant for conventional medicinal purposes using a toxicity test study. Methods: The acute toxicity test of the ethanolic extract of E. bulbosa Urb (EEEB) used the method adopted from the Organization Economic Cooperation and Development (OECD) guidelines 425 for testing Wistar rats. Results: During 14 days of the acute toxicity study, there were no significant changes in rat weight, no mortality, and no signs of toxicity after the oral EEEB administration at 2000 mg/kg body weight (bw). The limit test showed that the LD50 of EEEB was higher than 2000 mg/kg bw. Conclusion: EEEB has low toxicity because its LD50 is higher than the limit test results.

Safety Evaluation of Eucalyptus globulus Essential Oils through Acute and Sub-acute Toxicity and Skin Irritation in Mice and Rats

2020 ◽  
Vol 14 (3) ◽  
pp. 187-195
Author(s):  
Berhan Mengiste ◽  
Tizazu Zenebe ◽  
Kassahun Dires ◽  
Ermias Lulekal ◽  
Awol Mekonnen ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Essential Oil ◽  
Eucalyptus Globulus ◽  
Biochemical Parameters ◽  
Skin Irritation ◽  
Toxicity Study ◽  
Subacute Toxicity ◽  
Acute Toxicity Study ◽  
Limit Test ◽  
Ointment Formulation

Background: The Eucalyptus globulus extractions have been used by the traditional healers to treat diseases in the study area. Our previous study revealed that the essential oil has antimicrobial and antifungal activity. This study determined phytochemical analysis, skin irritation, acute and subacute toxicity of Eucalyptus globulus essential oil in mice and rats. Methods: The phytochemicals were analyzed using GC-MS mass spectrometry. The acute toxicity study was determined at three dose levels of 1500 mg/kg, 1750mg/kg, and 2000 mg/kg. The essential oil limit test at a dose of 1000 mg/kg was administered to mice for 28 consecutive days for sub-acute toxicity study. The mice mortality, behavioral change, injury and other signs of illness were recorded once daily. Biochemical parameters were evaluated. Liver and kidney were analyzed for histopathological analyses. The 5% ointment formulation was applied to the rat skin to determine skin irritation effects. Results: The Eucalyptus globulus essential oil showed no effect on the mice at a dose of 1500mg/kg and below, but caused signs of toxicity and death at a dose of 1750mg/kg and above compared to the controls (p<0.05). The LD50 value was 1650 mg/kg. There was no significant difference (p > 0.05) in the body weights, gross abnormalities of the organs and biochemical parameters compared to the control at 1000 mg/kg subacute toxicity study. No histopathological changes were detected in the organs tested. The 5% ointment formulation did not show any abnormal skin reaction. Discussion: In the present study, the Eucalyptus globulus essential oil was comparable with other studies in terms of both chemical composition and its effects on sub-acute and topical application. Conclusion: This toxicity study demonstrated that Eucalyptus globulus essential oil is nontoxic at a relatively lower concentration.

Acute Toxicity Study of Herbal Ovulation Inducer Product ‘Estrofarm’ in Wistar Rats

2011 ◽  
Vol 4 (5) ◽  
pp. 528-529
Author(s):  
M. M. Gatne M. M. Gatne ◽  
◽  
M.J Saxena M.J Saxena ◽  
K. Ravikanth K. Ravikanth ◽  
A. Thakur A. Thakur
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study

scholarly journals ANTIHYPERLIPIDEMIC AND HEPATOPROTECTIVE STUDIES ON LEAVES OF MACARANGA TANARIUS

2016 ◽  
Vol 10 (1) ◽  
pp. 239
Author(s):  
Phebe Hendra ◽  
Fenty Fenty ◽  
Jeffry Julianus ◽  
Oktariani Aurelia Jamil ◽  
Dian Ayu Maharani ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Toxicity Study ◽  
Lipoprotein Cholesterol ◽  
Acute Toxicity Study ◽  
Macaranga Tanarius ◽  
Serum Glutamate ◽  
Ethanol Fraction

ABSTRACTObjective: This study investigated the antihyperlipidemic and hepatoprotective effects of the hexane-ethanol fraction of methanol extract ofMacaranga tanarius (HEM) in rats.Methods: The hexane-ethanol fraction was screened for toxicity by oral acute toxicity study. The antihyperlipidemic effect of the hexane-ethanolfraction and the unsolved of the hexane-ethanol fraction is measured against Wistar rats induced by glucose-fructose diets for 42 days throughmeasuring serum cholesterol, triglyceride (TG), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and fastingblood glucose. The hepatoprotective effect of the hexane-ethanol fraction is determined against Wistar rats with liver damage induced by carbontetrachloride through measuring serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkalinephosphatase (ALP), albumin, lactate dehydrogenase (LDH), and total bilirubin.Results: There is no toxic effect that was observed on acute toxicity study. The TG, LDL-c, and fasting blood glucose levels were significantly (p<0.05)reduced after both of treatment the hexane-ethanol fraction and the unsolved HEM. Administration of the hexane-ethanol fraction 68.6 mg/kgBWsignificantly (p<0.05) prevented elevation of SGPT, SGOT, LDH, ALP, and decreasing of albumin level.Conclusion: The study showed antihyperlipidemic and hepatoprotective activities of the HEM in animal models.Keywords: Macaranga tanarius, Antihyperlipidemic, Hepatoprotective.

Acute Toxicity Study and Faecal Dropping Capability of Ethanolic Extract of Tecoma stans in Albino Rats

Pharmacologia ◽  
2013 ◽  
Vol 4 (7) ◽  
pp. 464-468 ◽  
Author(s):  
S. Kameshwara ◽  
C. Jothimaniv ◽  
R. Senthilkum ◽  
S. Thenmozhi ◽  
R. Sundaragan ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Toxicity Study ◽  
Albino Rats ◽  
Acute Toxicity Study

scholarly journals Study the Acute & Sub Acute Toxicity of Ricinus cummunis Lnn. Ethanol Extract of Seed in Albino Mice

2018 ◽  
Vol 6 (02) ◽  
Author(s):  
Manal H. AL-Jborrey ◽  
Muastafa A. K. Altaie ◽  
Ayyad W. Al-Shahwany
Keyword(s):  
Acute Toxicity ◽  
Morphological Changes ◽  
Ricinus Communis ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Chronic Administration ◽  
Industrial Applications ◽  
Toxicity Study ◽  
Herbal Extract ◽  
Acute Toxicity Study

Background: Toxicity still a global problem for the environment, agriculture and ultimately human health. Objective: In this study attempt to investigate the toxicological profile of the ethanol, extract of Ricinus cummunis after acute and sub-chronic administration to mice. Methods: In the acute toxicity study, a single administration of the extract at doses of 1000,2000,3000,4000 and 5000 mg/kg, respectively, was gave orally. Mice were observed for general behavioral changes, adverse effects and mortality up to 10 days post-treatment. In sub-acute toxicity studies, herbal extract was gave orally to mice at doses of 50 mg/kg, 100 mg/kg and 150 mg/kg for 10 days. Results: In the acute toxicity study, the mortality appeared in 2000 mg/kg and LD50 were calculated at 1100 mg/kg. In the sub-chronic toxicity the study show significant differences in body weight between the control and treated groups (p < 0.05). Histopathology of vital organ (liver & kidney) show morphological changes. Conclusions: These results demonstrate the real toxic effect of the ethanolic extract after single dose. The LD50 value is 1100 mg/kg and research indicates that successive use of the seed at the dose above (2 g/kg in human) daily for long period may cause toxic signs. Highlights: The Ricinus communis oil's has wide variety of industrial applications: as a drying oil for paints, varnishes, plastics and resins is an ingredient in numerous cosmetics. But it need to toxicity study as acute and sub-acute with observation of hematological and histopathological to be more safety  for used.

scholarly journals Phytochemical and acute toxicity study of aqueous extract of Bambusa vulgaris leaves on Wistar rats

2020 ◽  
Vol 02 (04) ◽  
pp. 107
Author(s):  
Appy Simone Abe ◽  
Kouao Augustin Amonkan ◽  
Kacou Marius Jules Djetouan ◽  
Kesse Philippe N’Da
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Bambusa Vulgaris
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