scholarly journals Evaluation of antidiarrheal effect of combination of Salam Leaves (Syzygiumpolyanthum) and Jackfruit Leaves (Artocarpus heterophyllus Lam.) infusum in rats induced by castor oil

2021 ◽  
pp. 148-151
Author(s):  
Husnul Khuluq ◽  
Evi Marlina
Keyword(s):  
Experimental Study ◽  
Traditional Medicine ◽  
Castor Oil ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Intestinal Lumen ◽  
Control Group ◽  
Artocarpus Heterophyllus

Backround: Diarrhoea is a condition characterised by watery, loose stools that occurs more than three times daily. In Indonesian traditional medicine, salam leaves and jackfruit leaves have been used as herbal treatments for many conditions, including as antidiarrheal medicines. Aims: The aim of this experimental study was to evaluate the antidiarrheal effect of a combination of Salam leaves and jackfruit leaves infusum induced by castor oil in rats. Method: The rats were divided into nine groups, where the negative control group was given CMC 1 %, the positive control group was given tannins, and five test groups were given the infusum with five comparisons of each dose administered orally. Castor oil was used as a stimulant of diarrhoea. Results: The results show that with all combinations of salam leaves: jackfruit leaves have antidiarrheal effects with decreased frequency of defecation, faeces consistency, and faeces weight at ratios of 1:1, 1:2, 1:3, 2:1, and 3:1 compared to the negative control (p < 0.05). Phytochemical test of the infusum indicated positive tannins. The antidiarrheal effect of both infusums might be due to the presence of tannins, which have anti-secretory effect in the intestinal lumen. Conclusions: The treatment with combination of salam leaves and jackfruit leaves infusum in rats induced by Castor oil has an antidiarrheal effect. The best result is a mixture of salam infusum: jackfruit infusum with 3:1 ratio.

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

scholarly journals Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255533
Author(s):  
Victor Angelo Martins Montalli ◽  
Patrícia Rejane de Freitas ◽  
Milenna de Figueiredo Torres ◽  
Oscar de Figueiredo Torres Junior ◽  
Dienne Hellen Moutinho De Vilhena ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
High Rate ◽  
Positive Control Group ◽  
Control Group ◽  
Healthy Environment ◽  
The Mean ◽  
Petri Dishes ◽  
Uv C

Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x108 CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm2 of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

The Effect of Chemically Modified Tetracycline-3 on the Progression of Dental Caries in Rats

2018 ◽  
Vol 52 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Jun Xu ◽  
Congcong Miao ◽  
Zhenchuan Tian ◽  
Jinlu Li ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Dental Caries ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Caries Prevalence ◽  
Chemically Modified ◽  
Dentin Caries ◽  
Chemically Modified Tetracycline

Objective: Matrix metalloproteinases (MMPs) exist in human saliva and dentin and play an important role in the degradation of organic matrix in teeth. Chemically modified tetracycline-3 (CMT-3) is an inhibitor of MMPs. CMT-3 has been used experimentally to treat caries since 1999, but no distinction between dental caries prevalence and dentin caries prevalence has been described. Methods: A total of 65 Sprague-Dawley rats were randomly divided into three groups. The positive control group (25 rats) was inoculated with Streptococcus mutans (ATCC700610) and fed the cariogenic feed of improved Keyes Diet 2000. The CMT-3 group (25 rats) was also inoculated with S. mutans and fed the cariogenic feed of improved Keyes Diet 2000; the surfaces of rats’ molars were daily treated with 0.02% CMT-3. The negative control group (15 rats) was only fed the standard rodent chow. At the end of the 10th week, the dental caries prevalence and dentin caries prevalence of each group were calculated, and the regions of caries were assessed. Results: No caries was found in the negative control group. The dental caries prevalence of the CMT-3 and the positive control group was 75.0 and 83.3%, respectively (p > 0.05, Table 2). The dentin caries prevalence of the CMT-3 and the positive control group was 33.3 and 70.8%, respectively (p < 0.05, Table 2). The Keyes scoring of dentin caries in the CMT-3 group was significantly lower than that in the positive control group (p < 0.05, Table 3). Conclusions: CMT-3 had no effect on the prevalence of dental caries, but could lower the prevalence and slow down the progression of dentin caries.

Protective Effect of Curcumin on Carbapenem-Resistant Escherichia coli–Induced Lung Injury in Rats

2016 ◽  
Vol 101 (7-8) ◽  
pp. 304-312 ◽  
Author(s):  
Cagla Bali ◽  
Nejat Altintas ◽  
Ozlem Ozmete ◽  
Ibrahim Gelincik ◽  
Hakan Yabanoglu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lung Injury ◽  
Intraperitoneal Injection ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
E Coli ◽  
Carbapenem Resistant

Curcumin has remarkable anti-inflammatory and antioxidant properties. The aim of this study was to investigate the protective effects of curcumin on a rat model of carbapenem-resistant Escherichia coli–induced acute lung injury (ALI). Thirty-two rats were randomly allocated to 4 groups to induce an ALI: negative control group (rats not infected with E coli with no antibiotic treatment), positive control group (rats infected with E coli with no antibiotic treatment), imipenem group (rats infected with E coli that received intraperitoneal injection of imipenem), and the imipenem+curcumin group (rats infected with E coli that received intraperitoneal injection of imipenem and were fed on curcumin).The rats were killed, and lung tissues samples were harvested for biochemical analyses and histopathologic examination. Total antioxidant status (TAS), total oxidant status (TOS), tumor necrosis factor α (TNFα), and interleukin-6 (IL6) were measured. TOS increased in the positive control group (P &lt; 0.001) and decreased in the imipenem and imipenem+curcumin groups (P &lt; 0.001 and P &lt; 0.001, respectively). TAS decreased in the positive control group (P = 0.005). Imipenem treatment did not increase TAS, but the imipenem+curcumin group increased TAS (P = 0.014). TNFα and IL6 increased in the positive control group compared with the negative control group (P &lt; 0.001 and P = 0.010, respectively). Imipenem decreased TNFα (P &lt; 0.001), but did not decrease IL6 (P = 0.418). Imipenem+curcumin decreased TNFα (P &lt; 0.001); this decrease was more pronounced compared with the imipenem group (P = 0.008). IL6 decreased in the curcumin group compared with the positive control group (P = 0.011). Curcumin combined with imipenem can be an alternative therapeutic agent to overcome the resistance of E coli strains.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals The Effect of Tempeh Gembus Variations to Serum Levels of High Sensitivity C-Reactive Protein (hsCRP) and Serum Levels of Fibrinogen of Sprague Dawley Rats with Aterogenic Diet

2018 ◽  
Vol 25 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Partika Kharunia Dewi ◽  
Diana Nur Afifah ◽  
Ninik Rustanti ◽  
Mohammad Sulchan ◽  
Gemala Anjani
Keyword(s):  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Atherogenic Diet ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Significant Difference

Abstract Background and aims: Cardiovascular diseases are widespread and causes many deaths in the world. The concentration of acute phase protein: C-reactive protein (CRP) and fibrinogen will rise dramatically when inflammation happens, which that can be used as an early marker of cardiovascular disease risk. Tempeh gembus contains fiber, unsaturated fatty acids and isoflavones are believed to reduce the inflammatory reaction. The aim of the study was to determinate the effect of tempeh gembus variations to levels of hcCRP and levels of fibrinogen of Sprague Dawley rats with atherogenic diet. Material and methods: This study was quasi-experimental with posttest only randomized control group design using 35 Sprague Dawley mice. The rats were randomized into 5 groups: negative control group given the standard diet, the positive control group given standard diet and atherogenic diet, and three treatment groups were given the standard diet, atherogenic diet and variation of tempeh gembus (tempeh gembus, heated tempeh gembus and tempeh gembus with bromelain enzyme) for 28 days. Serum levels of hsCRP and fibrinogen examined using ELISA (Enzyme-linked Immunosorbent Assay). Results and conclusions: The administration of tempeh gembus with bromelain enzyme is the most effective treatment for hsCRP serum level indicated a significant difference (p=0.028) between the negative control group, positive control group and first group with the third group. Fibrinogen serum levels showed significant differences in all treatment groups (p =0.042), administration of tempeh gembus with bromelain enzyme is the most effective treatment is shown by a significant difference between the negative control group and the positive control group with third group. The administration of tempeh gembus with bromelain enzyme for 28 days can reduce the serum levels of hsCRP and fibrinogen on rats significantly.

scholarly journals Effectiveness of Boiled Comfrey Leaves (Symphytum Officinale L) on SGOT SGPT Serum in Male Wistar Strain Rats With Acute Hepatitis Models

2019 ◽  
Vol 7 (1) ◽  
pp. 756-767
Author(s):  
Rois Sahidin ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Symphytum Officinale ◽  
Wistar Strain

Acute hepatitis is an acute disease caused by viruses, alcohol and drugs, which is characterized by an increase in SGOT SGPT serum. The purpose of this study was to determine the effectiveness of boiled comfrey leaves (Symphytum Officinale L) levels of SGOT and SGPT serum in male wistar strain rats with acute hepatitis models. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. The objects were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 2.8 grams of comfrey (symphytum officinale L) leaves was given as much as 2.7 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare serum levels of SGOT & SGPT. The results showed there were significant differences in serum levels of SGOT between the treatment group, positive control and negative control (p <0.05) and there were significant differences in SGPT results between the treatment group and positive control group (p <0.05) but there were no significant differences between the SGPT levels of the treatment group and the negative control group (p = 0.173). As Conclusion, boiled Comfrey leaf has an effect in decreasing serum SGOT & SGPT in male wistar strain rats with acute hepatitis model.

scholarly journals Ameliorative Effect of Ethanolic Extract of Petroselinum Crispum Against Gentamicin-Induced Nephrotoxicity in Rabbits Male

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Sarah Hussain ◽  
Mariam Kadhem
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Positive Control Group ◽  
Control Group ◽  
Petroselinum Crispum

The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, haematological and biochemical parameters, gross lesion and histopathological changes. Twenty four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg orally for 15 days.The results of the gentamicin treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight a significantly declined (p≤ 0.05) compared other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to negative control group. On the other hand, the group treated with ethanolic extractof Petroselinum crispum at dose 125 mg/kg induced improved of parameters as recorded significant increased(P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significant decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250mg / kg) acts as potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

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