Optimization of preparation of rosehip tea from the area of Stara Planina Mt

Etnobotanika ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 57-69
Author(s):  
Danijela Kostić ◽  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Therapeutic Effects ◽  
Bioactive Substances ◽  
Rosa Canina ◽  
Maximum Extraction ◽  
High Antioxidant Activity ◽  
Medicinal Properties

Stara Planina Mt is rich in medicinal plants, that are natural sources of various bioactive substances and have many useful medicinal properties. Rosehip, the fruit of wild rose (Rosa canina L.) is very popular and widely used. There is extensive empirical evidence of its therapeutic effects. The high content of vitamins, organic acids, phenolic compounds, carotenoids and pleasant taste define it as a valuable dietary supplement. There are different procedures for making rosehip teas. In this paper, we have optimized the process of preparation of rosehip tea from the aspect of maximum extraction and preservation of vitamin C content in tea. The antioxidant activity of aqueous extracts of rosehips, depending on the extraction time and the temperature at which the extraction is performed, was also examined..The free radical scavenging activity of the plant extracts was analyzed by using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay. Optimal conditions are 30 minutes and a temperature of 40 °С. In this way, vitamin C is best extracted and its stability is preserved, which results in the high antioxidant activity of the tested teas.

scholarly journals Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

2021 ◽  
Vol 11 (8) ◽  
pp. 3634
Author(s):  
Teresa Leszczyńska ◽  
Barbara Piekło ◽  
Aneta Kopeć ◽  
Benno F. Zimmermann
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Total Polyphenols ◽  
Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

scholarly journals Polyphenolic Composition of Rosa canina, Rosa sempervivens and Pyrocantha coccinea Extracts and Assessment of Their Antioxidant Activity in Human Endothelial Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 92 ◽  
Author(s):  
Efthalia Kerasioti ◽  
Anna Apostolou ◽  
Ioannis Kafantaris ◽  
Konstantinos Chronis ◽  
Eleana Kokka ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Endothelial Cells ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Quinic Acid ◽  
Syringic Acid ◽  
Rosa Canina ◽  
Polyphenolic Composition ◽  
Total Polyphenolic Content ◽  
Gallic Acid Equivalent

The aim of the present study was the investigation of the antioxidant activity of plant extracts from Rosa canina, Rosa sempervivens and Pyrocantha coccinea. The results showed that the bioactive compounds found at higher concentrations were in the R. canina extract: hyperoside, astragalin, rutin, (+)-catechin and (-)-epicatechin; in the R. sempervirens extract: quinic acid, (+)-catechin, (−)-epicatechin, astragalin and hyperoside; and in the P. coccinea extract: hyperoside, rutin, (−)-epicatechin, (+)-catechin, astragalin, vanillin, syringic acid and chlorogenic acid. The total polyphenolic content was 290.00, 267.67 and 226.93 mg Gallic Acid Equivalent (GAE)/g dw, and the total flavonoid content 118.56, 65.78 and 99.16 mg Catechin Equivalent (CE)/g dw for R. caninna, R. sempervirens and P. coccinea extracts, respectively. The extracts exhibited radical scavenging activity in DPPH and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)•+ assays and protection from ROO•-induced DNA damage in the following potency order: R. canina > R. sempervirens > P. coccinea. Finally, treatment with R. canina and P. coccinea extract significantly increased the levels of the antioxidant molecule glutathione, while R. canina extract significantly decreased Reactive Oxygen Species (ROS) in endothelial cells. The results herein indicated that the R. canina extract in particular may be used for developing food supplements or biofunctional foods for the prevention of oxidative stress-induced pathological conditions of endothelium.

Antihemolytic and antioxidant activities of Allium paradoxum

2010 ◽  
Vol 5 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Mohammad Ebrahimzadeh ◽  
Seyed Nabavi ◽  
Seyed Nabavi ◽  
Bahman Eslami
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dependent Manner ◽  
Vitro Assay ◽  
Aerial Parts

AbstractAntioxidant activity of the aerial part and bulbs of Allium paradoxum was investigated by eight in vitro assay systems. Extracts showed good antioxidant activity. IC50 for 1,1-diphenyl-2-picryl hydrazyl radical-scavenging activity was 890.9±43.2 and 984.9±33.5 µg/ml for the aerial part and bulbs, respectively. The aerial parts have better reducing power than bulb extracts but not comparable with Vitamin C (P>0.001). Extracts showed weak Fe2+ chelating ability, the IC50 being 959±47 and 530±24 µg/ml for bulbs and aerial parts, respectively. Both tested extracts exhibited good hydrogen peroxide scavenging in a concentration dependent manner. They exhibited good antioxidant activity against the hemoglobin-induced linoleic acid system that was comparable with vitamin C (P>0.01). They showed good activity against cumene hydro peroxide induced hemolysis in RBCs. In addition, they possessed antihemolytic activity. The extract from aerial parts had significantly higher total phenol and flavonoid content than did bulbs. Amounts of eight elements (Cu, Mn, Zn, Fe, Ni, Pb, Cd and Cr) were also determined in the bulb and aerial part using atomic absorption spectroscopy. They contained higher Fe and Mn contents than other elements.

Antioxidant Activities of Yinchenhao (Artemisiae Capillaris) Leaves

2020 ◽  
Vol 18 (4) ◽  
pp. 386-391
Author(s):  
Yonghun Kim ◽  
Ang Li ◽  
Junyu Wang ◽  
Wancong Yu ◽  
Fang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Therapeutic Effects ◽  
Scavenging Activity

Yinchenhao (Artemisia capillaris Thunb.) is a pharmaceutical agent that not only shows therapeutic effects against hepatobiliary diseases but also offers various physiological benefits. This study examined the extraction rates of antioxidant extracts from Yinchenhao leaves using different solvents. The extraction rates using different solvents were as follows: ethanol (12.1 ± 0.87%) > water (7.7 ± 0.45%) > n-butanol (1.3 ± 0.16%) ethyl acetate > (1.3 ± 0.14%) > n-hexane (1.1 ± 0.15%). The n-butanol and ethyl acetate extracts showed higher 1,1-Diphenyl-2 picrylhydrazyl radical free radical scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging activity and ferric ion reducing antioxidant activity, while n-hexane extracts showed weak antioxidant activity. In conclusion, Yinchenhao leaf has potential as a natural antioxidant, and n-butanol and ethyl acetate may be effective extraction solvents for studying its antioxidant activities.

HPTLC analysis and in vitro antioxidant activity of aqueous bark extract of Erythrina variegata L.

2016 ◽  
Vol 63 (3) ◽  
pp. 143-157 ◽  
Author(s):  
Kanakasabapathy Devaki
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Erythrina Variegata ◽  
High Antioxidant Activity ◽  
Layer Chromatography

Erythrina variegata L. is an important medicinal plant used in the preparations of Ayurvedic formulations used against several ailments. This study was carried out to investigate the presence of secondary metabolites using phytochemical screening, high-performance thin-layer chromatography (HPTLC) fingerprinting analysis and the antioxidant potential of the aqueous bark extract of E. variegata L. The secondary metabolites and the free radical scavenging activity were analyzed using standard protocols. The results obtained in the present study revealed that E. variegata has high antioxidant activity against free radicals based on phytoconstituents.

scholarly journals Antioxidant Activity of a Geopropolis from Northeast Brazil: Chemical Characterization and Likely Botanical Origin

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Joselena M. Ferreira ◽  
Caroline C. Fernandes-Silva ◽  
Antonio Salatino ◽  
Dejair Message ◽  
Giuseppina Negri
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Decisive Role ◽  
Northeast Brazil ◽  
Total Phenols ◽  
Botanical Origin ◽  
Plant Resin ◽  
High Antioxidant Activity

Geopropolis is a product containing wax, plant resin, and soil particles. It is elaborated by stingless bees of tribe Meliponini. Methanol extracts of sample of geopropolis produced by Scaptotrigona postica (“mandaguari”) in the state of Rio Grande do Norte (RN, northeast Brazil) were analyzed for the determination of standard parameters (total phenols, total flavonoids, and radical scavenging activity) and chemical characterization by HPLC-DAD-MS/MS analysis. The sample analyzed has high contents of total phenols and flavonoids, as well as high antioxidant activity. The constituents characterized were mainly flavonols, such as quercetin methyl ethers, and methoxychalcones. Such chemical profile is similar to the composition of a green propolis from the same area of RN, which is produced by Africanized Apis mellifera, using shoot apices of Mimosa tenuiflora, popularly known as “jurema-preta.” This finding provides evidence that “mandaguari” geopropolis and honeybee propolis have the same botanical origin in RN. The sharing of a plant resin source by phylogenetically distant bees (Apinae and Meliponinae) suggests that bee genetic factors play little role in the choice of plants for resin collection and that the availability of potential botanical sources plays a decisive role.

scholarly journals Variations in the L-DOPA Content, Phytochemical Constituents and Antioxidant Activity of Different Germlines of Mucuna pruriens (L.) DC.

2021 ◽  
Vol 33 (8) ◽  
pp. 1881-1890
Author(s):  
B. Rakesh ◽  
K. Hima Bindu ◽  
N. Praveen
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Mucuna Pruriens ◽  
Metal Chelating ◽  
Biochemical Assays ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Commercially Important ◽  
Potential Activity

In this study a ‘wonder plant’ Mucuna pruriens (L.) DC., which is commercially important medicinal plant of the Fabaceae family known for its treatment in Central Nervous System disorders like Dementia, Parkinson’s, Alzheimer’s, etc. have been selected. Different germplasms have been collected to analyze the phytochemical variations between them and quantify the L-DOPA in root, stem, leaves and seeds of all the five germlines using HPLC. Along with the biochemical assays, antioxidant activity by DPPH, phosphomolybdneum method, the metal chelating and reductive potential activity of all the germplasms were studied. All parts of the plant have shown the presence of L-DOPA but, seeds have the highest quantity followed by the roots, stem and leaves. Arka Shubra seeds showed high L-DOPA content (51.9 mg/g) while the other germplasms showed L-DOPA ranging from 43-45 mg/g. Highest content of carbohydrates (258.8 mg/g) and phenolics (157.0 mg/g) was seen in the seeds of Arka Aswini. While the seeds and leaves of Arka Charaka showed high protein (332.2 mg/g) and flavonoid (10.2 mg/g) content, respectively. High proline (1.74 mg/g) was observed in the seeds of Arka Shubra. Antioxidant studies revealed that Arka Charaka and Arka Daksha to be having high reductive power and free radical scavenging activity by phosphomolybdate method while high metal chelating activity was observed in Arka Aswini (88.7%) and high antioxidant activity by DPPH method was seen in Arka Shubra (86.5%).

scholarly journals A Comparative Assessment on Antioxidant and Phytochemical of Trichilia monadelpha (Thonn) J.J. De Wilde (Meliaceae) Plant Extracts

2021 ◽  
pp. 24-33
Author(s):  
P. D. Clark ◽  
E. Omo-Udoyo
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Hydroxy Radical ◽  
Reducing Ability

This study was designed to evaluate the phytochemical screening and antioxidant activities of Trichilia monadelpha plant extracts. The leaves were extracted using two different solvents namely-(n-hexane and methanol).  The dry or wet yield of n-hexane extract was 1.3%, while methanolic extract exhibited a percentage yield of 3.7 %.  Phytochemical research revealed the presence of secondary plant metabolites such as; alkaloids, flavonoids, cardiac glycosides, terpenoids and saponins. In-vitro antioxidant activity was determined using three assays (DPPH free radical scavenging assay, reducing ability and hydroxy radical scavenging activity) with four concentrations (0.25, 5.0, 1.0 and 2.0 mg/L), vitamin C was also used as a standard antioxidant. The percentage of inhibition was measured, and the results from all assay models showed a concentration-dependent percentage of inhibition by the methanol extract. However, the percentage values for the 2,2, -diphenyl-1-picrylhydrazyl (DPPH), reducing ability and hydroxy radical scavenging activity assays of T. monadelpha leaf was lower than that of standard vitamin C. The Pearson’s correlation coefficient was evaluated and the results showed that of the two extracts, the methanolic extract had the most antioxidant activity with the methanol extract exhibiting a better significant correlation that had a similar trend to that of the antioxidant compound (vitamin C). The results of this study have shown that the plant Trichilia monodelpha contains bioactive compounds which may have contributed to its antioxidant properties.

scholarly journals Seasonal Variations of Phytochemical Content and Antioxidant Activity of Senna italica Leaves

2020 ◽  
Vol 32 (9) ◽  
pp. 2371-2374
Author(s):  
SIBUSISO C. MNCUBE ◽  
SECHENE S. GOLOLO ◽  
MOTETELO A. MOGALE
Keyword(s):  
Antioxidant Activity ◽  
Seasonal Variations ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Phytochemical Content ◽  
Four Seasons ◽  
Maximum Benefit ◽  
High Antioxidant Activity

Senna italica is a widely used medicinal plant well known for their laxative properties and high antioxidant activity, as well as possession of a variety of bioactive compounds. For its optimum usage, the current study was aimed at the determination of seasonal variations in the phytochemical content and antioxidant activity of its leaves. The leaves of Senna italica were collected during each of the four seasons (autumn, winter, spring, summer) in the year 2018. Total phenolic, total tannin, total flavonoid and total saponin contents of the leaves were determined using UV spectrophotometric method. Antioxidant activity was evaluated using the DPPH free radical scavenging activity and H2O2 reducing activity assays. Total phenolic content was recorded in higher amounts during autumn; total tannin content was in higher amounts during autumn and summer; total flavonoids were higher during autumn and summer and total saponins were higher in summer. High antioxidant activity strength demonstrated through relatively lower IC50 was shown in summer and autumn which, was consistent with the relative phytochemical contents. The current results suggest that the optimum period of harvest for maximum benefit regarding the antioxidant activity of Senna italica leaves is between summer and autumn and that its antioxidant activity strength is aligned to the accumulation of the antioxidant compounds.

scholarly journals Abrus precatorius Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vanitha Reddy Palvai ◽  
Sowmya Mahalingu ◽  
Asna Urooj
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Effect Of Temperature ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Plant Materials ◽  
Oil Emulsion ◽  
Abrus Precatorius

Natural antioxidants present in foods and other biological materials have attracted considerable interest because of their presumed safety and potential nutritional and therapeutic effects. Antioxidant constituents of plant materials act as radical scavengers and convert the radicals to less reactive species. Abrus precatorius (AP) was analyzed for its proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent-induced oxidation in oil emulsion and microsomes. In addition, the effect of temperature (100∘C, 15, and 30 min) and pH (4.5, 7, and 9) C on the antioxidant activity of ME was investigated. The leaves were rich in total polyphenols, flavonoids, β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose-dependent manner. The AP exhibited more inhibition of oxidation in microsomes (73%) than compared to oil emulsion (21%). Heat treatment resulted in an increase of radical scavenging activity of extract (28% to 43%). At pH 4.5 the extract exhibited more antioxidant activity and stability compared to pH 7 and 9. Data indicates that potential exists for the utilization of Abrus precatorius as a natural antioxidant.

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