scholarly journals KHẢO SÁT CÁC YẾU TỐ ẢNH HƯỞNG ĐẾN QUÁ TRÌNH SẢN XUẤT LOVASTATIN TỪ NẤM Asperillus terreus EV8 BẰNG PHƯƠNG PHÁP LÊN MEN BÁN RẮN

2021 ◽  
Vol 5 (1) ◽  
pp. 2207-2216
Author(s):  
Nguyễn Phạm Tuấn ◽  
Bằng Hồng Lam ◽  
Nguyễn Phạm Tú
Keyword(s):  
Carbon Source ◽  
Abiotic Factors ◽  
Bone Diseases ◽  
Synthesis Process ◽  
Medium Ph ◽  
Fermentation Time ◽  
Medium Carbon ◽  
Statin Group ◽  
Fermentation Method ◽  
Potential Sources

Lovastatin một loại thuốc thuộc nhóm statin và được sử dụng để hạ cholesterol. Lovastatin cũng được sử dụng điều trị bệnh tim mạch vành, bệnh Alzheimer và các bệnh về xương,…. Nấm Asperillus terreus được xem là một trong những nguồn tổng hợp lovastatin, trong quá trình tổng hợp lovastatin chịu ảnh hưởng của nhiều yếu tố sinh học và phi sinh học. Nghiên cứu được thực hiện nhằm đánh giá ảnh hưởng của các yếu tố đến quá trình sản xuất lovastatin từ nấm bằng phương pháp lên men bán rắn. Các yếu tố ảnh hưởng đến quá trình lên men từ nấm Asperillus terreus EV8 như cơ chất, pH, nguồn carbon, nguồn nitrogen và thời gian lên men được đánh giá. Hàm lượng lovastatin được xác định bằng phương pháp đo quang ở bước sóng λ=238 nm. Kết quả nghiên cứu cho thấy, nấm Asperillus terreus EV8 sản xuất lovastatin tốt nhất dưới điều kiện như cơ chất (gạo trắng), pH môi trường (pH = 6), nguồn carbon (glucose 5 g/L), nguồn nitrogen (pepton 5 g/L) và thời gian lên men (8 ngày), hàm lượng lovastatin đạt 4,66 mg/g. ABSTRACT Lovastatin is a drug belonging to statin group and is used to reduce cholesterol. Lovastatin is also applied to treat coronary heart disease, Alzheimer's disease, and bone diseases. Asperillus terreus is considered as one of the potential sources of lovastatin, but the lovastatin synthesis process affected by various biological and abiotic factors. The study was conducted to evaluate the effect of factors on the production of lovastatin from this type of fungi by solid state fermentation method. The effect of factors on the production of lovastatin from Asperillus terreus EV8 as substrates, pH of medium, carbon source, nitrogen and fermentation time were investigated. Lovastatin assay was determined by spectrophotometer at 328 nm. The results showed that Asperillus terreus EV8 strains produced lovastatin under conditions as substrates (rice), pH of medium (pH = 6), carbon source (glucose 5 g/L), nitrogen source (peptone 5 g/L) and fermentation time (8 days) and the amount of lovastatin reaches 4.66 mg/g.  

scholarly journals STUDI PEMANFAATAN KULIT KOPI ARABIKA (Coffee arabica L.) SEBAGAI MIKRO ORGANISME LOKAL (MOL)

AGROINTEK ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
A Ita Juwita ◽  
Arnida Mustafa ◽  
Risna Tamrin
Keyword(s):  
Food Source ◽  
Material Decomposition ◽  
Brown Sugar ◽  
Fermentation Time ◽  
Fermentation Method ◽  
Local Material ◽  
Coffee Arabica ◽  
Phosphorus And Potassium ◽  
Macro Nutrients

<span class="fontstyle0">MOL (Mikro Organisme Lokal) is the result of local material decomposition with<br />fermentation method. MOL contains macro and micro nutrients and microbial decomposers.<br />The smaller coffee peel that will fermented, the faster MOL can be formed. To accelerate<br />decomposition in MOL solution, it can be added food source of bacteria such as coconut water<br />and brown sugar.<br />The purpose of this study was to study the process of making MOL from coffee peel, to<br />analyze the content of nitrogen, phosphor, and potassium of MOL of coffee peel and to<br />determine the effect of fermentation time on nitrogen, phosphor, and potassium contents of<br />MOL that resulted.<br />This study was conducted with 4 (four) treatments, week 1, 2, 3 and 4 and 1 factor and<br />using 2 times replicate. The parameter of testing used for making coffee peel as local<br />microorganism are MOL volume, nitrogen, phosphor and potassium contents.<br />The result of study showed that the highest mol volume obtained in week 4 of 8.5 ml and<br />the lowest in week 1 of 5 ml. They were obtained from 500 g coffee peel. Determination of<br />nitogen, phosphorus and potassium as a macro nutrients has been conducted. The result showed<br />that the highest nitrogen was found to be 0,0039% in week 1, 3 and 4 and the lowest in week 2<br />with percentage 0.034 %. Determine of phosphorus showed that the highest phosphor was<br />obtained in week 2 wtih percentage 0.033 % and the lowest in week 4 of 0.018%, and the result<br />of potassium analyze, the highest obtained in week 2 of 0.035 % and the lowest in week 4 of<br />0.014%</span>

Study on Ecological Carbon Sink Regionalization of Land Use in Shenzhen City

2013 ◽  
Vol 726-731 ◽  
pp. 4730-4737
Author(s):  
Kei Mei Hu ◽  
Wei Ling Liu ◽  
Jing Hai Zhu ◽  
Lin Wang ◽  
Lin Bo Zhang ◽  
...  
Keyword(s):  
Land Use ◽  
Carbon Source ◽  
Greenhouse Gas Emission ◽  
Carbon Sink ◽  
Carbon Sources ◽  
Urban Land ◽  
Urban Land Use ◽  
Medium Carbon ◽  
Source Regions ◽  
Shenzhen City

This paper is to propose a method of ecological carbon sink regionalization in the view of GHG (greenhouse gas) emission reduction, which could provide scientific theory basis for development and utilization of urban land resources, ecological environment construction, biodiversity conservation, the industry layout and formulation of the regional sustainable development strategy. Based on the existing researches foundation, this study takes Shenzhen as a typical case to put forward a method for carbon sink regionalization of urban land use with further quantitative evaluation of ecosystem carbon storage (density), carbon sources/sinks function and carbon sink potential of Shenzhen city. The results show that: Shenzhen ecological carbon sink is regionalized into six categories 55 regions, including 15 intensive carbon sink regions, 17 medium carbon sink regions, 10 weak carbon sink regions, 4 carbon neutral regions, 5 medium carbon source regions and 4 intensive carbon source regions.

scholarly journals Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology

2016 ◽  
Vol 20 (1) ◽  
pp. 53-64 ◽  
Author(s):  
He Chen ◽  
Jianbo Kou ◽  
Man Hu ◽  
Guowei Shu
Keyword(s):  
Response Surface Methodology ◽  
Carbon Source ◽  
Central Composite Design ◽  
Liquid Medium ◽  
Nitrogen Source ◽  
Bifidobacterium Bifidum ◽  
Optimal Conditions ◽  
Medium Carbon ◽  
Central Composite ◽  
Mrs Medium

Abstract In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01.

Efficient recovery of carbon, nitrogen, and phosphorus from waste activated sludge

2013 ◽  
Vol 68 (4) ◽  
pp. 916-922 ◽  
Author(s):  
Yinguang Chen ◽  
Xiong Zheng ◽  
Leiyu Feng ◽  
Hong Yang
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Precipitation Method ◽  
Waste Activated Sludge ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Fermentation Time ◽  
Fermentation Liquid ◽  
Efficient Recovery ◽  
Biological Nitrogen

Carbon, nitrogen, and phosphorus need to be recovered to reduce the environmental impact of waste activated sludge (WAS). In this study the improved short-chain fatty acid (SCFA) production from WAS by the addition of kitchen waste to adjust the ratio of carbon to nitrogen (C/N), and the efficient recovery of nitrogen and phosphorus from the fermentation liquid were reported. Firstly, the optimum conditions for SCFA production were found to be pH 8, temperature 35 °C, C/N ratio 21 mg-C/1 mg-N, and fermentation time 6 d, using the response surface methodology. After alkaline fermentation, the struvite precipitation method was applied to efficiently and simultaneously recover the released ammonia and phosphorus from the fermentation liquid. Finally, the fermentation liquid was used as the additional carbon source for biological nitrogen and phosphorus removal. It was observed that, compared with acetic acid, the use of fermentation liquid as carbon source showed greater removal efficiencies of total nitrogen and total phosphorus.

Isolation and Identification of a Mold Strain Producing Esterifying Synthetase and Study on its Metabolies

2011 ◽  
Vol 236-238 ◽  
pp. 2460-2463
Author(s):  
Dan Huang ◽  
Yu Long Chu ◽  
Zhi Chao Shang ◽  
Yu Long Li ◽  
Zhong Ming Lu
Keyword(s):  
Carbon Source ◽  
Isoamyl Alcohol ◽  
Carbon Chain ◽  
Butyl Alcohol ◽  
Culture Conditions ◽  
Identification System ◽  
Culture Temperature ◽  
Automated Identification ◽  
Isolation And Identification ◽  
Fermentation Time

The metabolies characterization of mold producing esterifying synthetase of different culture conditions were studied. A mold strain producing esterifying enzyme was isolated from Luzhou-flavor DaQu and it was identified by Biolog Microbes automated identification System. Culture conditions such as culture temperature, fermentation time and carbon source were changed. The fermentatiom broth was extracted by ethanol and analyzed by GC-MS. The results showed that the strain was Aspergillus flavus. When the culture temperature increased, the molecular weight of its metabolites increased, the synthesis of high alcohol increased too, and their carbon chain lengthened gradually. When incubation time was prolonged, the varying law of the synthesis of its metabolites was alcohols-acid-ester, the metabolites with longer carbon chain produced earlier than the metabolites with shorter carbon chain. When the carbon source were starch, sucrose and bran, n-butyl alcohol and isoamyl alcohol were the common metabolites. When the carbon source was sucrose, most of the metabolites were alcohol and acid with shorter carbon chain. When the carbon source was starch, diethyl succinate was produced. When the carbon source was bran, the metabolites were less.

Technology Optimization on Preparation of Peanut Antioxidant Peptides by Bacillus Subtilis Solid State Fermentation Method

2016 ◽  
Vol 835 ◽  
pp. 103-108 ◽  
Author(s):  
Li Na Yu ◽  
Ting Ting Xu ◽  
Chu Shu Zhang ◽  
Jie Sun ◽  
Jie Bi
Keyword(s):  
Bacillus Subtilis ◽  
Free Radicals ◽  
Solid State ◽  
Solid State Fermentation ◽  
Water Bath ◽  
Soluble Nitrogen ◽  
Antioxidant Peptides ◽  
Fermentation Time ◽  
Fermentation Method ◽  
Nutrient Salt

The purpose of this paper is to investigate the preparation of peanut antioxidant peptides by Bacillus subtilis solid state fermentation method and to provide a theoretical basis for further investigating deep processing products of fermentation peanut meal. The preparation technics for peanut antioxidant peptides optimized with the soluble nitrogen concentration of peptides, 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and hydroxyl free radicals scavenging rates of fermentation broth as the indexes of investigation including the cell age was 20h, bacterial suspension volume was 3.0mL, fermentation temperature was 40°C, nutrient salt solution was 15mL, the fermentation time was 42h, water bath temperature was 45°C, and water bath time was 3h. The research shows that IC50 values for peptides scavenging of DPPH, hydroxyl and superoxide anion free radicals, iron and copper ion chelating rates, lipid peroxidation inhibiting rate, iron and molybdenum reducing activity were 3.48mg/mL, 6.24mg/mL, 2.06mg/mL, 0.54mg/mL, 1.19mg/mL, 4.56mg/mL, 9.58mg/mL and 2.17mg/mL, respectively.

scholarly journals Yeast Concentration, pH, and Fermentation Time on the Production and Concentration of Bioethanol Made from Sargassum crassifolium as a Renewable Energy Source

2020 ◽  
Vol 9 (1) ◽  
pp. 50
Author(s):  
Hasan Tuaputty
Keyword(s):  
Economic Value ◽  
Raw Material ◽  
Cellulose Content ◽  
Marine Waters ◽  
Production Potential ◽  
Medium Ph ◽  
Fermentation Time ◽  
Pharmaceutical Industries ◽  
High Production ◽  
Yeast Concentration

Sargassum crassifolium is one type of brown algae which has many benefits and has a high economic value. It has a relatively short harvest age. It is widespread in Indonesia's marine waters with high production potential, but the production is still largely from the harvest of natural supplies. Sargassum contains a lot of alginate polysaccharides that are used for food-beverage, cosmetics, and pharmaceutical industries. In addition, it also contains other types of polysaccharides namely cellulose (part of the cell wall), manithol (as stored carbohydrates) and fucoidan. The cellulose content in Sargassum ranges from 23.97 to 35.22%. Thus, it can be processed into a raw material in the production of bioethanol. The results of the research showed that the yeast concentration 3%, the medium pH 7, and fermentation time 72 hours gave the best results in increasing the production and content of bioethanol made from Sargassum crasifolium.

scholarly journals Bio-lipid production from crude glycerol and lignocellulosic hydrolysate with oleaginous yeasts

2020 ◽  
Author(s):  
Mikolaj Chmielarz ◽  
Johanna Blomqvist ◽  
Sabine Sampels ◽  
Mats Sandgren ◽  
Volkmar Passoth
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Paper Industry ◽  
Lipid Production ◽  
Strain Differences ◽  
Hemicellulose Hydrolysate ◽  
Lipid Concentration ◽  
Oleaginous Yeasts ◽  
Glycerol Conversion ◽  
Fermentation Time

Abstract Background: Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low- value side-products of biodiesel transesterification and pulp- and paper industry, respectively, which can be converted to microbial lipids by oleaginous yeasts. This study aimed to test the ability of oleaginous yeasts to utilise CG and HH and mixtures of them as carbon source. Results: Eleven out of 27 tested strains of oleaginous yeast species were able to grow in plate tests on CG as sole carbon source. Among them, only one ascomycetous strain, belonging to Lipomyces starkeyi , was identified, the other 10 strains were Rhodotorula spec. When yeasts were cultivated in mixed CG/ HH medium, we observed an activation of glycerol conversion in the Rhodotorula strains, but not in L. starkeyi . Two strains - Rhodotorula toruloides CBS 14 and Rhodotorula glutinis CBS 3044 were further tested in controlled fermentations in bioreactors in different mixtures of CG and HH. The highest measured average biomass and lipid concentration were achieved with R. toruloides in 40% HH medium mixed with 60 g/L CG - 19.4 g/L and 10.6 g/L, respectively, with a lipid yield of 0.22 g lipids per consumed g of carbon source. Fatty acid composition was similar to other R. toruloides strains and comparable to that of vegetable oils. Conclusions: There were big strain differences in the ability to convert CG to lipids, as only few of the tested strains were able to grow. Lipid production rates and yields showed that mixing GC and HH have a stimulating effect on lipid accumulation resulting in shortened fermentation time to reach maximum lipid concentration, which provides a new perspective on converting these low- value compounds to biolipids.

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