Relationship Between Hepatotoxicity That Induced by CCL4 and Regucalcin Protein Marker

2021 ◽  
Vol 2 (2) ◽  
pp. 35-44
Author(s):  
Murtadha M. Jawad
Keyword(s):  
Cell Death ◽  
Carbon Tetrachloride ◽  
Chemical Compounds ◽  
The Body ◽  
Protein Marker ◽  
Inhibiting Factors ◽  
Functional Nature ◽  
Harmful Substances ◽  
Dna Strand ◽  
Extent Of Damage

This review deals with the world's most important problem, which is hepatotoxicity. As a result of the distinctive location of the liver and its effect on all the substances entering the body and manufactured from it, the wastes resulting from metabolism, as well as its functional nature in the equation and removal of toxins. Therefore, the liver is one of the most influential and first organs in the human body because of the major functions it shows. Therefore, most of the research targets the liver through a number of chemicals, including carbon tetrachloride. Carbon tetrachloride is widely used, and its work depends on destroying hepatocytes through a group of chemical reactions with a group of chemical compounds for the liver such as fats and proteins, thus producing harmful substances such as free radicals, whose damage depends on the concentration and period of exposure to carbon tetrachloride. In order to determine the extent of the influence of the liver, there must be evidence. Our choice of Regucalcin was because it is closely related to several physiological functions, including: its role in maintaining the level of calcium on a regular basis in addition to its anti-programmed effects of cell death by inhibiting factors that break down the DNA strand by inhibiting the action of a group of enzymes. And other factors that destroy cells. Thus, the presence of Regucalcin inside and out of cells is evidence of the extent of damage and damage to hepatocytes and their dissolution. Therefore, Regucalcin can be considered a criterion for assessing the extent and degree of damage to the liver.

Amelioration of Carbon Tetrachloride-Induced Liver Injury by Citrus Leaf Extract

2019 ◽  
Vol 17 (4) ◽  
pp. 426-431
Author(s):  
Jin Xuezhu ◽  
Li Jitong ◽  
Nie Leigang ◽  
Xue Junlai
Keyword(s):  
Carbon Tetrachloride ◽  
Leaf Extract ◽  
Hepatic Injury ◽  
The Body ◽  
Dependent Manner ◽  
Weight Changes ◽  
Citrus Leaf ◽  
Dose Dependent ◽  
And Oxidative Stress

The main purpose of this study is to investigate the role of citrus leaf extract in carbon tetrachloride-induced hepatic injury and its potential molecular mechanism. Carbon tetrachloride was used to construct hepatic injury animal model. To this end, rats were randomly divided into 4 groups: control, carbon tetrachloride-treated, and two carbon tetrachloride + citrus leaf extract-treated groups. The results show that citrus leaf extract treatment significantly reversed the effects of carbon tetrachloride on the body weight changes and liver index. Besides, treatment with citrus leaf extract also reduced the levels of serum liver enzymes and oxidative stress in a dose-dependent manner. H&E staining and western blotting suggested that citrus leaf extract could repair liver histological damage by regulating AMPK and Nrf-2.

Synthesis of a silymarin-gold nanoparticle conjugate and analysis of its liver-protecting activity

2021 ◽  
Vol 22 ◽  
Author(s):  
Sergey Staroverov ◽  
Sergey Kozlov ◽  
Alexander Fomin ◽  
Konstantib Gabalov ◽  
Alexey Volkov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Liver Disease ◽  
Carbon Tetrachloride ◽  
Glutathione Depletion ◽  
Laboratory Animals ◽  
Experimental Hepatitis ◽  
Acute Liver Disease ◽  
The Mean ◽  
Extent Of Damage ◽  
First Time

Background: The liver disease problem prompts investigators to search for new methods of liver treatment. Introduction: Silymarin (Sil) protects the liver by reducing the concentration of free radicals and the extent of damage to the cell membranes. A particularly interesting method to increase the bioavailability of Sil is to use synthesized gold nanoparticles (AuNPs) as reagents. The study considered whether it was possible to use the silymarin-AuNP conjugate as a potential liver-protecting drug. Method: AuNPs were conjugated to Sil and examine the liver-protecting activity of the conjugate. Experimental hepatitis and hepatocyte cytolysis after carbon tetrachloride actionwere used as a model system, and the experiments were conducted on laboratory animals. Result: For the first time, silymarin was conjugated to colloidal gold nanoparticles (AuNPs). Electron microscopy showed that the resultant preparations were monodisperse and that the mean conjugate diameter was 18–30 nm ± 0.5 nm (mean diameter of the native nanoparticles, 15 ± 0.5 nm). In experimental hepatitis in mice, conjugate administration interfered with glutathione depletion in hepatocytes in response to carbon tetrachloride was conducive to an increase in energy metabolism, and stimulated the monocyte–macrophage function of the liver. The results were confirmed by the high respiratory activity of the hepatocytes in cell culture. Conclusion: We conclude that the silymarin-AuNP conjugate holds promise as a liver-protecting agent in acute liver disease caused by carbon tetrachloride poisoning.

Influence of Free Surface Environment on the Shear Zone in Metal Cutting

1966 ◽  
Vol 181 (1) ◽  
pp. 687-705 ◽  
Author(s):  
P. L. Barlow
Keyword(s):  
Free Surface ◽  
Carbon Tetrachloride ◽  
Strain Hardening ◽  
Shear Zone ◽  
Chemical Reaction ◽  
Cutting Forces ◽  
Metal Cutting ◽  
The Body ◽  
Surface Barrier ◽  
Rehbinder Effect

It has previously been suggested that the reduction in cutting forces obtained by the presence of fluids such as CCl4 on the backface or free surface of the forming chip was due to diffusion of the fluid into the body of the chip in the region of the shear zone. In the present work, experiments with carbon tetrachloride tagged with carbon-14 and with carbon tetrachloride tagged with chlorine-36 were performed with the object of assessing the extent of diffusion of lubricants into the chip when present on the free surface only. The results obtained disprove former hypotheses and suggest that the reduced cutting force is due solely to chemical reaction at the surface of the chip. Confirmation of the sensitivity of the surface of the deforming shear zone to change in surface condition was obtained by removing metal from this region by an electropolishing technique during slow speed cutting. By varying the electropolishing conditions increased or decreased cutting forces could be obtained. It is proposed that the result both of chemical reaction at the surface and of surface removal is to reduce the strain-hardening rate of the metal undergoing shear by reducing the surface barrier to the flow of dislocations out of the metal. The association of the surface reaction of carbon tetrachloride with a change in the strain-hardening characteristics of the metal in the shear zone leads to a classification of the backface phenomenon as a Rehbinder effect and enables this effect to be more closely defined than was hitherto possible. Evidence is also presented which indicates that the backface effect does not contribute to the reduction in cutting forces during rakeface lubrication and is therefore unimportant in practice where flood lubrication of the cutting region invariably occurs.

Tumor Microenvironment-Triggered In-Situ Cancer Vaccines with Dual Immunogenic Cell Death Inductions for Elevated Antitumor and Antimetastatic Therapy

Nanoscale ◽  
2021 ◽  
Author(s):  
Jun Lin ◽  
Binbin Ding ◽  
Pan Zheng ◽  
Dong Li ◽  
Meifang Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Microenvironment ◽  
Immune Responses ◽  
Cancer Immunotherapy ◽  
Cancer Vaccines ◽  
High Specificity ◽  
The Body ◽  
Immunogenic Cell Death ◽  
Specific Antigens

Cancer vaccine is to make tumor-specific antigens into vaccines, which then are injected back into the body to activate immune responses for cancer immunotherapy. Despite the high specificity and therapeutic...

Impact of nanomaterials on intestinal mucosal barrier and its application in treating intestinal diseases

2021 ◽  
Author(s):  
Wenshuai Hao ◽  
Ruitao Cha ◽  
Mingzheng Wang ◽  
Pai Zhang ◽  
Xingyu Jiang
Keyword(s):  
The Body ◽  
Mucosal Barrier ◽  
Intestinal Homeostasis ◽  
Intestinal Mucosal Barrier ◽  
Intestinal Diseases ◽  
Harmful Substances

The intestinal mucosal barrier (IMB) is one of the important barriers to prevent harmful substances and pathogens from entering the body environment and maintain intestinal homeostasis. The dysfunction of IMB...

scholarly journals Identification of Free Radical in Main Stream Cigarette Smoke in Cigarette with Clove mix (Kretek) and Cigarette without Clove mix (White)

2020 ◽  
Vol 21 (2) ◽  
pp. 73
Author(s):  
Ni Kadek Nova Anggarani ◽  
D.J. Djoko H.S. ◽  
Unggul P. Juswono
Keyword(s):  
Cigarette Smoke ◽  
Combustion Process ◽  
Chemical Compounds ◽  
Current Data ◽  
Main Stream ◽  
A Value ◽  
Harmful Substances ◽  
Free Gases ◽  
Smoke Sample ◽  
Landé Factors

Cigarette smoke is a source of free radicals. Cigarette smoke is a component of Aerosols and free gases that contain large amounts of chemical compounds. Cigarette smoke can be divided into two: main smoke and side smoke. The main smoke of cigarettes inhaled by smokers is known to cause a buildup of harmful substances and causes of oxidative stress so that research is carried out using ESR Leybold Heraeus. The sample used is a smoke sample from two types of cigarettes that are popular in Indonesia. The measurement is done by pulling smoke directly into the measurement tube. Then the resonance pattern is observed and the external magnetic field is calculated and the value of the lande factor is based on frequency and current data. Measurements result showed resonance in both types of cigarettes, at a frequency of 32.4-70.8 MHz and obtained values of lande factors that vary and have a value between 1.9-2.1 with two acquisition values that are outside the range that is 1.8368; and 2.2060. There are differences in the number of resonances that occur for each sample. This difference can be due to differences in the constituent of content and the texture of cigarettes which can affect the results of the combustion process.

scholarly journals Review of Chemical toxicity in Cosmetic products w.s.r. Dushi Visha

2019 ◽  
Vol 7 (07) ◽  
Author(s):  
Shubhangi Karanje
Keyword(s):  
Coal Tar ◽  
Chemical Compounds ◽  
The Body ◽  
The Self ◽  
Synthetic Method ◽  
Cosmetic Products ◽  
Chemical Toxicity ◽  
External Application ◽  
Self Confidence ◽  
Concept Of Beauty

 The concept of beauty and cosmetic is as old as mankind and civilization. Cosmetic product for females like sun scream, lipsticks, facial cream, nail paints, deodorants and sindoor are very popular in the market. They are generally the combination of various chemical compounds and some of them are derived from natural sources and others from synthetic method. In females regular external application of cosmetic products to improve beauty and appearance of a person which increases the self confidence. Most of the cosmetic products contains hazardous chemicals like talcum, parabens, coal, tar dye, phthalates, fragrance, tri ethanolamine and use of some heavy metals e.g. lead, mercury etc. Peoples are not aware of adverse effect of regular use of cosmetic products it accumulates in the body can cause contact dermatitis, skin disorders, allergies, hair loss and effect on nails etc. In Ayurveda cosmetic toxicity is compare to cumulative type of toxicity, so the cumulative type of toxicity is similar to Dushi Visha describes in Ayurveda.

scholarly journals IDENTIFIKASI SENYAWA GARLISIN DAN KUERSETIN SEBAGAI SENYAWA ANTIMIKROBA PADA SEDIAAN BASAH DAN KERING BAWANG PUTIH (Allium sativum L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 176
Author(s):  
Fanny Thresia Yunus ◽  
Ari Suwondo ◽  
Martini Martini
Keyword(s):  
Traditional Medicine ◽  
Allium Sativum ◽  
Chemical Compounds ◽  
The Body ◽  
Garlic Extract ◽  
Antimicrobial Compounds ◽  
Vacuum Oven ◽  
Garlic Powder ◽  
Three Samples

Indonesian people have long used garlic as traditional medicine because it contains various chemical compounds that are beneficial to the body. In this study identification of garlicin and kuersetin compounds in garlic as an antibacterial. This study requires 500 grams of garlic each in wet preparations consist of fine garlic and garlic extract, and dry preparation consists of garlic powder. In addition, this research requires 2,1 liters of 96% ethanol. Garlic is dried at 400C with TMI Vacuum Oven and extracted at 250C. Garlic powder is made using Krisbow dry cabinet with a temperature of 1000F and a pressure of 10 Pa. Garlisin content was identified by GCMS Shimadzu TQ8030 and FTIR Perkin Elmer Spectrum Two UATR, while identification of quercetin using Shimadzu HPLC type UFLC. The result shows that the compound garlicin (C6H10S2) was found in the three samples with identical similarity levels. In addition, the three samples showed differences in kuersetin content. The highest kuersetin content was 458.729 ppm garlic extract while the lowest kuersetin content was 81.181 ppm fresh garlic. Both of these compounds have potential as antimicrobial compounds.

scholarly journals Myocardial Damage by SARS-CoV-2: Emerging Mechanisms and Therapies

Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1880
Author(s):  
Huyen Tran Ho ◽  
Stefan Peischard ◽  
Nathalie Strutz-Seebohm ◽  
Karin Klingel ◽  
Guiscard Seebohm
Keyword(s):  
Immune Response ◽  
Cell Death ◽  
Energy Metabolism ◽  
Programmed Cell Death ◽  
Viral Replication ◽  
Ion Homeostasis ◽  
Membrane Vesicles ◽  
Cardiac Cells ◽  
The Body ◽  
Double Membrane

Evidence is emerging that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect various organs of the body, including cardiomyocytes and cardiac endothelial cells in the heart. This review focuses on the effects of SARS-CoV-2 in the heart after direct infection that can lead to myocarditis and an outline of potential treatment options. The main points are: (1) Viral entry: SARS-CoV-2 uses specific receptors and proteases for docking and priming in cardiac cells. Thus, different receptors or protease inhibitors might be effective in SARS-CoV-2-infected cardiac cells. (2) Viral replication: SARS-CoV-2 uses RNA-dependent RNA polymerase for replication. Drugs acting against ssRNA(+) viral replication for cardiac cells can be effective. (3) Autophagy and double-membrane vesicles: SARS-CoV-2 manipulates autophagy to inhibit viral clearance and promote SARS-CoV-2 replication by creating double-membrane vesicles as replication sites. (4) Immune response: Host immune response is manipulated to evade host cell attacks against SARS-CoV-2 and increased inflammation by dysregulating immune cells. Efficiency of immunosuppressive therapy must be elucidated. (5) Programmed cell death: SARS-CoV-2 inhibits programmed cell death in early stages and induces apoptosis, necroptosis, and pyroptosis in later stages. (6) Energy metabolism: SARS-CoV-2 infection leads to disturbed energy metabolism that in turn leads to a decrease in ATP production and ROS production. (7) Viroporins: SARS-CoV-2 creates viroporins that lead to an imbalance of ion homeostasis. This causes apoptosis, altered action potential, and arrhythmia.

scholarly journals Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

2014 ◽  
Vol 58 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Aneta Strachecka ◽  
Grzegorz Borsuk ◽  
Jerzy Paleolog ◽  
Krzysztof Olszewski ◽  
Milena Bajda ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Body Surface ◽  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chemical Compounds ◽  
The Body ◽  
Surface Chemical ◽  
Surface Compounds ◽  
Honey Bee Workers

Abstract Body-surface chemical compounds were studied in 1-day-old nest workers and foragers both in Buckfast and Caucasian bees. The workers of these two age-castes were sampled twice in each of two consecutive years. Body-surface lipids were determined by means of gas chromatography, with a GCQ mass spectrometer. Protein concentrations and activities on the body surface were examined in bee cuticle rinsings obtained from worker bees according to the methods of Lowry, of Anson, and of Lee and Lin. Protease and protease inhibitor activities were determined. Polyacrylamide gel electrophoresis was performed. Caucasian bees, particularly foragers, had more lipids, but Buckfast bees (two age-castes) had more proteins on their body surfaces. A total of 17 alkane types (C17 - C33), 13 alkene types (C21 - C33), 21 esters (C12 - C32), and a phenol (C14) were detected in both races. Alkene C33 was detected only in Caucasian bees. More alkanes, esters, and phenols were found in Caucasian 1-day-old nest workers and foragers than in these age-castes of Buckfast bees. The protein concentration and protease inhibitor activities were lower in Caucasian bees that had higher protease activities. These values corresponded with specific numbers and widths of the electrophoretic bands.

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