scholarly journals Comparative Study of the Antioxidant Activity of the Plasma of Healthy Subjects and those with Uterine Myomas by Scavenging the ABTS Radical+

2022 ◽  
pp. 1-6
Author(s):  
N’Negue Mezui-Mbeng MA ◽  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Uterine Myoma ◽  
Uterine Myomas ◽  
Total Antioxidant ◽  
Free Radical Activity ◽  
Over 40 Years ◽  
Effect Of Age ◽  
By Products

Introduction: The oxidative stress and the deterioration of the antioxidant system demonstrated by the dosage of antioxidant enzymes and the by-products of lipid peroxidation are involved in the occurrence of uterine myomas. Objectives: To measure the antioxidant activity of the plasma of patients with uterine myoma and that of the plasma of their age-matched controls by trapping the free radical ABTS+. The study aims to confirm by another assessment technique. Results: The anti-free radical activities of the plasmas of patients with myomas and those of their respective controls vary from 1.3 to 6%. In addition, two cases were observed: either the anti-radical activity of the control subjects turned out to be slightly higher than that of the patients; or the anti-free radical activity of patients with uterine myomas was slightly stronger than those of their controls. In addition, our results showed that the anti-free radical activity of patients aged over 40 years is slightly higher than that of patients aged less than or equal to 40 years. Finally, the anti-free radical activity decreases with an increase in BMI and increases with the number of gestities and parity. Conclusion: Assessment of the total antioxidant capacity of blood by trapping the ABTS+ radical can be used to assess, at a certain threshold, the oxidative stress involved in the development of uterine myoma. This method can also be used to assess the effect of age, BMI, pregnancy and parity on the occurrence of myomas.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

The state of the blood antioxidant system in the patients presenting with acromegaly

2015 ◽  
Vol 61 (2) ◽  
pp. 8-11
Author(s):  
M V Faassen ◽  
M S Pankratova ◽  
N N Molitvoslovova ◽  
A A Baizhumanov ◽  
S S Kovalenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Antioxidant System ◽  
The State ◽  
Total Antioxidant Activity ◽  
Sod Activity ◽  
Protein Thiols ◽  
Total Antioxidant

The state of the blood antioxidant system in the patients presenting with acromegaly. The objective of the present study was to evaluate the total antioxidant activity (TAA) of blood plasma, the levels of non-protein thiols and 2-thyobarbituric acid-active products (TBA-AP), superoxide dismutase (SOD) and catalase (CAT) activities as well as ceruloplasmin (CP) level in the patients presenting with acromegaly. It was shown that plasma TAA and SOD activity in this patients was on the average 20 and 30% lower respectively than in the control subjects. At the same time, the TBA-AP and CP levels increased by 50 and 40% respectively. These data suggest the development of oxidative stress in the acromegalic patients.

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

scholarly journals Antioxidant Potential and Peroxidative State of `Golden Smoothee' Apples Treated with 1-Methylcyclopropene

2006 ◽  
Vol 131 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Rosa Vilaplana ◽  
M. Carme Valentines ◽  
Peter Toivonen ◽  
Christian Larrigaudière
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Storage Period ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
Pulp Tissue ◽  
Antioxidant Metabolism ◽  
Beneficial Effects ◽  
Total Antioxidant

In order to determine the effects that 1-methylcyclopropene (1-MCP) may have on antioxidant metabolism during cold storage, apples (Malus ×domestica Borkh. cv. Golden Smoothee) were treated with 625 nL·L−1 1-MCP immediately after harvest and stored in air for 3 months. Differences in total antioxidant activity and ascorbate levels were determined during storage and related to the activity of the antioxidant enzymes superoxide dismutase [SOD (EC 1.15.1.1)], catalase [CAT (EC 1.11.1.6)], and peroxidase [POX (EC 1.11.1.7)] in pulp. The level of oxidative stress in the pulp tissue was also established by determining changes in levels of hydrogen peroxide and in the content of peroxidative markers during storage. Controls and 1-MCP-treated fruit exhibited similar changes in total antioxidant activity and ascorbate levels. However, significant differences in oxidative stress levels were found between treated and untreated fruit. 1-MCP-treated fruit exhibited lower levels of hydrogen peroxide and significantly lower levels in peroxidative markers, especially at the end of the storage period. In line with this last result, 1-MCP-treated fruit also exhibited greater enzymatic antioxidant potential and, more specifically, a higher level of POX activity. Collectively, these results showed that 1-MCP did not detrimentally affect the antioxidant potential of the fruit and provided evidence to support the hypothesis that the beneficial effects of 1-MCP on ripening are not exclusively limited to its effect on ethylene, but also include direct effects on peroxidation and POX enzyme activity.

scholarly journals Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

2019 ◽  
Vol 4 (1) ◽  
pp. 14-18
Author(s):  
Nadezhda A. Kurashova ◽  
Ekaterina A. Kudeyarova ◽  
Ekaterina O. Kuznetsova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Antioxidant Protection ◽  
Defense System ◽  
Total Antioxidant ◽  
Peroxidation Processes

Background.Today infertile marriage is not only a serious medical, but also a socio-demographic and economic problem. Male factor contributes averagely to half of the cases of the disease in couples. Such factors as high levels of reactive oxygen species and oxidative stress have been reported to compromise the process of spermatogenesis and sperm function in men. Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality.Aims.To study the characteristics of the intensity of the processes of lipoperoxidation and antioxidant status in the ejaculate of men with different variants of spermograms.Materials and methods.We examined 69 men with primary infertility and 155 fertile men. The content of lipid peroxidation components and antioxidant protection was determined by spectrophotometric method.Results.The results of the study in men with infertility and asthenozoospermia showed decreased total antioxidant activity of sperm by 50 % and α-tocopherol by 52 %, and in men with infertility and oligozoospermia, decreased total antioxidant activity of sperm by 47 % and α-tocopherol by 41 %.Conclusions.The analysis indicates a change in the parameters of the system of lipid peroxidation – antioxidant defense system and confirms the development of oxidative stress in them. Depending on the pathological state of the ejaculate in men of reproductive age, lipid peroxidation processes have their own characteristics. In men with oligozoospermia, peroxidation processes occur more intensively. Activation of lipid peroxidation – antioxidant defense system processes can be both a consequence and a cause of various metabolic changes in the human body.

scholarly journals Protective Role of Ce Nanoparticles Against the Hepatotoxicity Induced by Exposure to Paraquat

2018 ◽  
Vol 6 (2) ◽  
pp. 37-43
Author(s):  
Hamid Heidary Dartoti ◽  
Farzin Firozian ◽  
Sara Soleimani Asl ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Role ◽  
Male Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Serum Samples ◽  
Tissue Samples ◽  
Total Antioxidant ◽  
Group 2

Objectives: The present study aimed to investigate the antioxidant activity of cerium oxide nanoparticles (CeNPs) against paraquat (PQ)-induced liver injury in rats. Methods: Thirty-two male rats were divided into four 8-member groups and treated intraperitoneally with PQ and/or CeNPs for 14 days. Group 1 received PQ (5 mg/kg/d), group 2 received CeNPs (15, 30, and 60 mg/kg/d), group 3 received a combination of PQ (5 mg/kg/d) and CeNPs (15, 30, and 60 mg/kg/d), and group 4 (control group) received saline solution. Serum samples along with liver tissue samples were collected from all the rats. Oxidative stress (OS) biomarkers including total antioxidant capacity, lipid peroxidation, total thiol groups, DNA damage, and nitric oxide levels were determined. Histological samples were also analyzed using hematoxylin and eosin staining slides. Results: Levels of oxidative stress and hepatic tissue damage were significantly higher in the PQ group compared to the control group. CeNPs at a dose of 15 mg/kg showed the antioxidant activity and compromised the PQ-induced damage. Conclusion: In the scenario tested in this study, CeNPs could reduce the levels of OS, as well as hepatic damage induced by PQ.

scholarly journals Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on certain seminal plasma traits of Hy – line laying breeder roosters subjected to oxidative stress induced by hydrogen peroxide

2012 ◽  
Vol 36 (0A) ◽  
pp. 62-69
Author(s):  
Hazim J. Al – Daraji
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Seminal Plasma ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

This study was conducted to evaluate the effect of adding different levels of rocket salad seeds powder to the diet on seminal plasma traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed into 5 treatments with 3 replicates each. Each replicate constituted of 4 roosters (12 roosters for each treatment). Experimental treatments were as following: T1: Males fed control diet and normal water, T2: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide (0.5%) / litter of water, T3: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.5 ml hydrogen peroxide (0.5%) / litter of water, T4: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 1 ml hydrogen peroxide (0.5%) / litter of water, and T5: Males fed control diet and drink tap water supplemented with 1 ml hydrogen peroxide (0.5%) / litter of water. Males were treated with hydrogen peroxide (6%) and rocket salad for 12 weeks starting from 59 week of male ages. Results revealed that treated the roosters with hydrogen peroxide without adding rocket salad powder to the diet of these roosters (T5) resulted in highly significant (p< 0.01) decrease as regards concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) increase concerning concentrations of tyrosine and malondialdehyde as compared with control group (T1) and rocket salad powder treatments (T2, T3, T4) after 12 weeks of experiment. However, supplementing diet of roosters with rocket salad powder (T2, T3, T4) resulted in highly significant (p< 0.01) increase with relation to concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) decrease respecting concentrations of tyrosine and malondialdehyde as compared with (T5) In conclusion adding rocket salad powder to the diet of roosters had important role in limiting the negative effect of oxidative stress induced by hydrogen peroxide on seminal plasma quality of roosters. Therefore, dietary supplementation with rocket salad powder could be used as one of important tools for improving semen quality of roosters.

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