scholarly journals Influence of biotechnological parameters on the yield of macrostructures from unfertilized seed germs of diploid sugar beet

2021 ◽  
Author(s):  
O. A. Zinchenko ◽  
N. S. Zatserkovna ◽  
O. A. Ukrainets ◽  
A. V. Zabolotna
Keyword(s):  
Sugar Beet ◽  
Sodium Hypochlorite ◽  
Nutrient Medium ◽  
Source Material ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Infected Seed ◽  
Selection Number ◽  
Sterile Seed

Purpose. To determine the influence of biotechnological parameters on the yield of macrostructures from unfertilized seed germs of diploid sugar beet. Methods. Biotechnological, laboratory, analytical, statistical. Results. It was found that the use of 35% sodium hypochlorite solution at an exposure of 40 min allows to obtain from 73.13 to 75.83% of sterile seed germs. Exposure of 50 min allows to obtain the sterility of the source material from 83.58 to 85.39%. Sterilization of explants for 60 min allows to obtain sterility of the source material from 86.88 to 92.80%. The share of infected seed germs with increasing exposure decreased from 20.09–22.14 to 6.52–12.61%. The yield of macrostructures has been experimentally confirmed to significantly depend on breeding genotype and type of medium. The largest number of calluses (10–80%) was formed with the use of the Hamburg and Eveleg’s medium. With the use of he Murasige and Skoog’s medium, their share was 10–35%. Noteworthy, in breeding genotypes 07–181, 80% of genotypes formed buds and 35% formed calluses in the Hamburg and Eveleg’s medium. Of breeding genotypes 07–178, 55% of genotypes formed a callus and 80% buds. Conclusions. As a result of the conducted researches the influence of biotechnological parameters (exposure to 35% solution of sodium hypochlorite, type of nutrient medium) on the yield of macrostructures from unfertilized seed germs of diploid sugar beet was determined. It is optimal to carry out treatment with 35% sodium hypochlorite solution for 50–60 minutes, regardless of the selection number of sugar beet. To obtain macrostructures from unfertilized seed germs of diploid sugar beet, it is necessary to use the Hamburg and Eveleg’s medium for breeding genotypes 07–188, 07–178 and 07–181.

scholarly journals Safety assessment of an etidronate in a sodium hypochlorite solution: randomized double‐blind trial

2019 ◽  
Vol 52 (9) ◽  
pp. 1274-1282 ◽  
Author(s):  
N. V. Ballal ◽  
P. Gandhi ◽  
P. A. Shenoy ◽  
V. Shenoy Belle ◽  
V. Bhat ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Safety Assessment ◽  
Sodium Hypochlorite Solution ◽  
Double Blind Trial ◽  
Double Blind ◽  
Hypochlorite Solution ◽  
Blind Trial

Reduction of Microbial Pathogens during Apple Cider Production Using Sodium Hypochlorite, Copper Ion, and Sonication

2004 ◽  
Vol 67 (4) ◽  
pp. 766-771 ◽  
Author(s):  
STEPHANIE L. RODGERS ◽  
ELLIOT T. RYSER
Keyword(s):  
Sodium Hypochlorite ◽  
Copper Ion ◽  
Microbial Pathogens ◽  
Sodium Hypochlorite Solution ◽  
Escherichia Coli O157 ◽  
Hypochlorite Solution ◽  
Apple Cider ◽  
E Coli ◽  
Log Reduction ◽  
Pathogen Reduction

Sodium hypochlorite (100 ppm), copper ion water (1 ppm), and sonication (22 to 44 kHz and 44 to 48 kHz) were assessed individually and in combination for their ability to reduce populations of Escherichia coli O157:H7 and Listeria monocytogenes on apples and in apple cider. Commercial unpasteurized cider was inoculated to contain approximately 106 CFU/ml of either pathogen and then sonicated at 44 to 48 kHz, with aliquots removed at intervals of 30 to 60 s for up to 5 min and plated to determine numbers of survivors. Subsequently, whole apples were inoculated by dipping to contain approximately 106 CFU/g E. coli O157:H7 or L. monocytogenes, held overnight, and then submerged in 1 ppm copper ion water with or without 100 ppm sodium hypochlorite for 3 min with or without sonication at 22 to 44 kHz and examined for survivors. Treated apples were also juiced, with the resulting cider sonicated for 3 min. Populations of both pathogens decreased 1 to 2 log CFU/ml in inoculated cider following 3 min of sonication. Copper ion water alone did not significantly reduce populations of either pathogen on inoculated apples. However, when used in combination with sodium hypochlorite, pathogen levels decreased approximately 2.3 log CFU/g on apples. Sonication of this copper ion–sodium hypochlorite solution at 22 to 44 kHz did not further improve pathogen reduction on apples. Numbers of either pathogen in the juice fraction were approximately 1.2 log CFU/ml lower after being juiced, with sonication (44 to 48 kHz) of the expressed juice decreasing L. monocytogenes and E. coli O157:H7 populations an additional 2 log. Hence, a 5-log reduction was achievable for both pathogens with the use of copper ion water in combination with sodium hypochlorite followed by juicing and sonication at 44 to 48 kHz.

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

2019 ◽  
Vol 82 (8) ◽  
pp. 1290-1296
Author(s):  
Filipe C. Vitali ◽  
Lincon H. Nomura ◽  
Débora Delai ◽  
Dilma H. N. Henriques ◽  
Ana M. H. Alves ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Gutta Percha ◽  
Surface Changes

Microbiological Assay of Low Chlortetracycline Concentrations in Animal Feeds

1965 ◽  
Vol 48 (2) ◽  
pp. 271-277
Author(s):  
Anthony Abbey ◽  
David B Hewel
Keyword(s):  
Sodium Hypochlorite ◽  
Animal Feed ◽  
Microbiological Assay ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Animal Feeds ◽  
Low Levels

Abstract Satisfactory microbiological values were obtained for animal feeds containing low levels of cblortetracycline (CTC), 5.0–40g CTC/ton. Results were comparable when calculated against both compensating CTC standard curves prepared from blank feed extracts and laboratory-inactivated CTC feed extracts. The inactivation of CTC in animal feed extracts by 5.25% sodium hypochlorite solution and heat was investigated, and it appeared to offer a convenient alternative for preparing compensating CTC standard curves.

Sign in / Sign up

Export Citation Format

Share Document