scholarly journals Phytochemical and Antihypertensive Tests of Celery (Apium graveolens L.) and Garlic (Allium sativum L.) Formula

2021 ◽  
Vol 6 (7) ◽  
pp. 40-49
Author(s):  
Rida Rosa ◽  
Harrizul Rivai
Keyword(s):  
Blood Pressure ◽  
White Male ◽  
Cardiovascular Death ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Apium Graveolens ◽  
Test Material ◽  
The People ◽  
Pharmacological Test

Hypertension is also the leading cause of death worldwide. Treatment and control of hypertension help prevent cardiovascular death. Traditionally celery and garlic have been used as ingredients to lower high blood pressure by the people of South Sulawesi. Phytochemical testing was carried out to provide information about compounds' content in the formula of celery and garlic. Furthermore, the determination of the levels of alkaloids and saponins was carried out gravimetrically, the decision of the stories of phenols with a UV-Visible spectrophotometer, and the conclusion of the levels tannins with a UV spectrophotometer. Pharmacological testing using 25 white male rats were treated with doses of celery and garlic formula given to rats, namely 185 mg/ 200 g BW, 370 mg/ 200 g BW, 740 mg/ 200 g BW orally as test material. In the negative control, the rats were only given distilled water alone, and in the positive control, it was induced using 8% NaCl and 0.05% prednisone orally. The test solution was given for 21 days. The results of the phytochemical test showed that the celery and garlic ingredients contained positive alkaloids, saponins, phenols, tannins with levels of 1.8917 % alkaloids, 0.5885 % saponins, 1.6138 % phenols, and 1,3485 % tannins. Pharmacological test results showed systolic blood pressure and diastolic blood pressure decreased significantly (p < 0.05).

scholarly journals Hypotensive and heart rate-lowering effects in rats receiving milk fermented by specific Lactococcus lactis strains

2012 ◽  
Vol 109 (5) ◽  
pp. 827-833 ◽  
Author(s):  
J. C. Rodríguez-Figueroa ◽  
A. F. González-Córdova ◽  
H. Astiazaran-García ◽  
B. Vallejo-Cordoba
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Lactococcus Lactis ◽  
Ace Inhibitor ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Spontaneously Hypertensive ◽  
Lowering Effect

Previous studies have demonstrated that milk fermented by specific Lactococcus lactis strains significantly inhibits the activity of angiotensin I-converting enzyme (ACE). However, the relationship between the ACE inhibitor and its in vivo action has revealed discrepancies. Therefore, the aim of the present study was to investigate the antihypertensive and heart rate (HR)-lowering effect of milk fermented by specific L. lactis in a murine model. Spontaneously hypertensive male rats (271 (SD14) g) were randomised into four treatment groups that were orally administered with milk fermented by L. lactis NRRL B-50 571 or L. lactis NRRL B-50 572 at 35 or 50 mg protein/kg body weight (BW), respectively. Further, two more groups were fed with different solutions as controls: a saline solution as the negative control and Captopril™ (40 mg/kg BW), a proven ACE inhibitor, as the positive control. Blood pressure and HR were monitored by the tail-cuff method before the treatments and at 2, 4, 6 and 24 h post-oral administration. The results demonstrated that milk fermented by L. lactis NRRL B-50 571 as well as by L. lactis NRRL B-50 572 presented an important systolic and diastolic blood pressure- and HR-lowering effect. Thus, milk fermented by specific L. lactis strains may present potential benefits in the prevention and treatment of CVD associated with hypertension in humans.

scholarly journals EKSTRAK AIR TANAMAN SELEDRI (APIUM GRAVEOLENS) TERHADAP PENINGKATAN VOLUME URIN TIKUS PUTIH

2019 ◽  
Vol 10 (3) ◽  
pp. 1-6
Author(s):  
Adita Silvia Fitriana
Keyword(s):  
White Male ◽  
Urine Volume ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Apium Graveolens ◽  
Male Rat ◽  
Preparation Process ◽  
Diuretic Effect ◽  
Made In

ABSTRACT Processing of celery as diuretic is done by society in various ways. So far, the most effective preparation process of celery extract has not been known. This research aims to examine the diuretic effect of various celery extract preparation.  This research was conducted using celery water extract which is made in accordance with the people’s habit, using aquades as a negative control and HCT as a positive control. The effectiveness test of celery water extract as diuretic was done on white male rat by measuring the urine volume every hour for six hours. The results showed that the largest urine volume is obtained from the boiling celery extract.   Keywords: celery, diuretic, rat

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK ETANOL DAUN MIANA(COLEUS SCUTELLARIOIDES [L]) BENTH PADA TIKUS PUTIH JANTAN GALUR WISTAR (RATTUS NORVEGICUS)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 55
Author(s):  
Sri Gentari Benjamin ◽  
Adithya Yudistira ◽  
Henki Rotinsulu
Keyword(s):  
White Male ◽  
Data Retrieval ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Leaf Extracts ◽  
Antipyretic Effect ◽  
Hb Vaccine

ABSTRACTThis study aims to determine the antipyretic effect of miana leaves extract (Coleus scutellariodes L Bent) in white male rats strain wistar. The subjects of this study were 15 white male rats, which were divided into 5 groups, namely the negative control group (1% CMC), the positive control group (Paracetamol), and the treatment group (miana leaf extracts). This type of research is a laboratory experiment, which carried out on the test animals based on a Completely Randomized Design (CRD) by testing the antipyretic effect carried out by administering the DPT-Hb vaccine. The observed response was in the form of data retrieval related to rat temperature after 2 hours of DPT-hb vaccine administration. Body temperature measurements were carried out before administering the vaccine, then successively at the 30th, 60th, 90th, and 120th minutes, respectively. The results showed miana leaf extracts at a dose of 25 mg, 50 mg and 75 mg had an antiperetic effect in white male rats but the dose 75mg provides the most effective antipyretic effect. Thus it can be concluded that the ethanol extracts of miana leaves has an antipyretic effect in white male rats, which were induced orally.  Keywords: Antipyretics, Miana Leaves, Male White Rat. ABSTRAK Penelitian ini bertujuan untuk mengetahui Efek Antipiretik Ekstrak daun Miana (Coleus scutellariodes [L] Bent) pada Tikus Putih Jantan Galur Wistar. Subjek penelitian ini ialah 15 ekor tikus putih jantan yang dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (Parasetamol), dan kelompok perlakuan (ektrak daun miana).Jenis penelitian ini ialah eksperimental laboratorium yang akan dilakukan pada hewan uji berdasarkan Rancangan Acak Lengkap (RAL) dengan pengujian efek antipiretik dilakukan dengan cara memberikan vaksin DPT-Hb. Respon yang diamati yaitu berupa pengambilan data terkait suhu tikus setelah 2 jam pemberian vaksin DPT-hb. Pengukuran suhu tubuh dilakukan sebelum pemberian Vaksin, kemudian berturut-turut pada menit ke-30, 60, 90, dan 120. Hasil penelitian menunjukan ekstrak daun Miana dengan dosis 25 mg, 50 mg dan 75 mg memiliki efek antiperetik pada tikus putih jantan namun dosis 75 mg memberikan  efek antipiretik yang paling efektif. Dengan demikian dapat disimpulkan bahwa ekstrak daun miana memiliki efek antipiretik pada tikus putih jantan yang di induksi secara oral.  Kata kunci : Antipiretik, Daun Miana, Tikus Putih Jantan

scholarly journals Uji Potensi Nefropati Diabetes Daun Sirih Merah (Piper croatum Ruiz & Pav) pada Tikus Putih Jantan (Rattus norvegicus)

KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 239-251
Author(s):  
Joni Tandi ◽  
Rizaldy Lalu ◽  
Magfirah ◽  
Yunlis Silintowe Kenta ◽  
Ronaldy Nobertson
Keyword(s):  
Blood Glucose ◽  
White Male ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Average Dose ◽  
Average Value ◽  
Betel Leaf ◽  
White Rats

This study aims to determine the type of secondary metabolite compounds in red betel leaf ethanol extract, the potential of red betel leaf ethanol extract on blood glucose, creatinine, and urea levels of male white rats induced by streptozotocin. This study used 30 white male rats which were divided into 6 groups: normal control, negative control, positive control, the dose of 150, 250, and 350 mg/kg BW. The showed that results ethanol extract of red betel leaves contained alkaloids, flavonoids, phenols, saponins, and tannins. Ethanol extract of red betel leaf at a dose of 150 mg /kg BW is an effective dose in reducing levels glucose blood with an average value of decreased blood glucose levels of 238.25 mg/dL, and has an effect on creatinine and ureum, with an average dose of 350 mg/kg BW with a mean creatinine level of 0.64 mg/dL and mean ureum of 39.68 mg/dL. Keywords: Creatinine, ureum, red betel leaf, streptozotocin

scholarly journals The effect of Etlingera elatior J. flower on the pulmo histology of white rat (Rattus novergicus L.) after cigarette smoke exposure

2021 ◽  
Vol 948 (1) ◽  
pp. 012057
Author(s):  
F Hasri ◽  
K Manalu ◽  
E P S Tambunan ◽  
Syukriah
Keyword(s):  
Cigarette Smoke ◽  
White Male ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Cigarette Smoke Exposure ◽  
White Rats ◽  
Histological Improvement ◽  
Etlingera Elatior

Abstract Cigarette smoking is one of health issues that mainly harmed the lung due to its free radicals. Kecombrang (Etlingera elatior J.) is a medicinal plant known to have a high amount of antioxidant. This study aims to determine the effect of antioxidant in Etlingera elatior J. flower on the improvement of pulmonary histology of male white rats (Rattus novergicus L.) as the secondhand smoke (cigarette exposure). This research was conducted by experimental method with ethanol extract of Etlingera elatior J. flower and pulmonary organ of white male rats as the test materials. There were 25 white male rats that divided into 5 groups (Positive Control, Negative Control, P1: 30 mg/Kg, P2:60 mg/Kg, and P3:90 mg/Kg). The results were statistically analyzed using One Way ANOVA test and Duncan’s test. The results showed a decreased amount of damage at P1, P2 and P3 groups. The statistical analysis showed that Etlingera elatior J. flower extract had a significant effect (p <0.05) on the histological improvement in each group. Group P2 shows the lowest rate of damage that it can be concluded the most effective dose in repairing the damage to rat pulmonary histology due to exposure of cigarette smoke.

UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

2020 ◽  
pp. 68-73
Author(s):  
Yuni Asri Mulatsih Agami ◽  
Eka Wisnu Kusuma
Keyword(s):  
Oxidative Stress ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Experimental Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Negative Group ◽  
Ic50 Value ◽  
Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7%  Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7  setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif.    Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii  in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

scholarly journals Effect of Administration of Combination of Captopril and Celery Extract on Blood Pressure and Electrolyte Levels of Hypertensive Rats

2020 ◽  
Vol 7 (3) ◽  
pp. 81
Author(s):  
Siska Siska ◽  
Franciscus D. Suyatna ◽  
Abdul Mun'im ◽  
Anton Bahtiar
Keyword(s):  
Blood Pressure ◽  
Urine Volume ◽  
Concomitant Administration ◽  
Reduce Blood Pressure ◽  
Male Rats ◽  
Apium Graveolens ◽  
Hypertensive Rats ◽  
Treatment Groups ◽  
Urinary Potassium ◽  
Standard Value

Based on previous reports, the combination of captopril and celery could reduce blood pressure in hypertensive patients. This study aimed to investigate the changes of blood pressure, urine volume, sodium, and potassium level, due to concomitant administration of captopril with celery extract orally in male rats induced by 4% NaCl. The blood pressure was measured using a non-invasive tail method. The urine and blood were collected, and the sodium, potassium concentration, and cumulative urine volume were measured. The combination of 5 mg/kgBW of captopril and 40 mg/kgBW of celery extract decreased the blood pressure in hypertensive rats better than 5 mg/kgBW of captopril alone. The fell in blood pressure was followed by an increase in urine volume. Urinary and serum sodium, serum potassium levels tended to increase in all treatment groups, but not significantly different from the healthy group. Urinary potassium levels tended to decrease except in the combined 5 mg/kgBW of captopril and 40 mg/kgBW of celery extract. In conclusion, oral administration of a combination of 5 mg/kgBW captopril and 40 mg/kgBW celery extract decreased the blood pressure to the standard value in NaCl-induced hypertension rats.Keywords: Apium graveolens, captopril, celery, hypertension, pharmacodynamic

scholarly journals UJI DAYA ANTIINFLAMASI EKSTRAK ETANOLIK KULIT TERONG BELANDA (Solanum betaceum, Cav.) PADA MENCIT JANTAN YANG DIINDUKSI KARAGENIN

2020 ◽  
Vol 16 (02) ◽  
pp. 86
Author(s):  
Margareta Retno Priamsari ◽  
Rina Ayu Krismonikawati
Keyword(s):  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Test Material ◽  
Edema Formation ◽  
Flavonoid Compounds ◽  
The One ◽  
Post Hoc ◽  
Solanum Betaceum

ABSTRACT Inflammation is a normal response to injury due to release of chemical mediators such as prostaglandins. Solanum betaceum Cav. skin contains flavonoid compounds. This study aims to determine the antiinflammatory power and the effect of variations in the dose of ethanolic extract of the skin of Solanum betaceum Cav on Swiss male strain mice caragenin-induced. The ethanolic extract of Solanum betaceum Cav skin was obtained using remaseration method with 96% ethanol solvent. Antiinflammatory power test using edema formation method with induction of caragenin 1% intraplantar 30 minutes before administration of the test material. Tests were carried out on 25 mice records which were divided into 5 groups namely negative control (0.5% CMC Na suspension), positive control (diclofenac sodium suspension in 0.5% CMC Na dose 7 mg/kgBW) and given suspension respectively. Dutch eggplant skin ethanolic extract in CMC Na 0.5% dose 70 mg/kgBW; 140 mg/kgBW; 280 mg/kgBW orally. The parameters observed were edema thickness measured using calipers at 30 minute intervals for 360 minutes. The results of edema thickness difference are used to calculate the Area Under Curve (AUC) and the percentage of antiinflammatory power (% AIP). Dat a were analyzed statistically with the One Way Anova test and continued by the Post Hoc Test with a 95% confidence level. The results showed that the ethanolic extract of Solanum betaceum Cav skin contains flavonoid compounds and has antiinflammatory power. The greater the dose of ethanolic extract of Solanum betaceum Cav skin, the greater the anti-inflammatory power. Keywords: Solanum betaceum Cav., antinflammation, caragenin

scholarly journals PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

2017 ◽  
Vol 13 (3) ◽  
Author(s):  
Condro Suro Miyarso ◽  
Tri Cahyani Widiastuti ◽  
Naelaz Zukhruf Wakhidatul Kiromah
Keyword(s):  
Body Weight ◽  
Testosterone Level ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Group Iii ◽  
Eurycoma Longifolia ◽  
Group I ◽  
Eurycoma Longifolia Jack

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight.  Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

scholarly journals Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

2021 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
Alvyan Lantang Anugrah ◽  
Hana Eliyani ◽  
Budi Utomo ◽  
Suherni Susilowati ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Sertoli Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Beet Root ◽  
White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

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