scholarly journals Cloning and Expression Analysis of bZIP Transcription Factor from Finger Millet (Eleusine coracana L.) Under Abiotic Stress Conditions

2022 ◽  
Vol 9 (1) ◽  
pp. 1-13
Author(s):  
Jenifer Lolita C
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Expression Analysis ◽  
Finger Millet ◽  
Abiotic Stress Tolerance ◽  
Eleusine Coracana ◽  
Stress Conditions ◽  
Cloning And Expression

Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic stress tolerance. In this study, we were attempted to study characterization of bZIP, a transcription factor from a climate smart cereal finger millet (Eleusine coracana L.). Seeds of Eleusine coracana (finger millet) was purchase from local market and were grown under field conditions drought and salt stress conditions. In this study, EcbZIP gene was isolated from finger millet, cloned into DH5α cells, screened by using colony PCR and expression analysis in response to two abiotic stresses was carried out by using qRT PCR. EcbZIP coding DNA sequence and protein sequence were retrieved from NCBI Nucleotide Database and Genpept of Accession number KP033192.1 and AJP67539.1 and validated by using SMART (simple modular architecture tool) Domain Tool. Cloning and expression studies were carried out using standardized molecular biology protocol. Results depicted that EcbZIP transcription factor showed significant upregulation under both salt and drought stress conditions, indicating that it plays an important role in tolerance towards these stresses. In conclusion, expression analysis of bZIP gene from finger millet seed cultivar ML-365 showed 5-fold upregulation to salt stress to drought stress and 8-fold upregulation to salt stress. Hence, it can serve as a candidate gene for improving abiotic stress tolerance and can be helpful in enhancing the crop productivity under stress conditions.

scholarly journals TheSsDREBTranscription Factor from the Succulent HalophyteSuaeda salsaEnhances Abiotic Stress Tolerance in Transgenic Tobacco

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Xu Zhang ◽  
Xiaoxue Liu ◽  
Lei Wu ◽  
Guihong Yu ◽  
Xiue Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Soluble Sugars ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Dreb Transcription Factor

Dehydration-responsive element-binding (DREB) transcription factor (TF) plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designatedSsDREB, was isolated from succulent halophyteSuaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed thatSsDREBwas significantly induced under salinity and drought stress. Overexpression ofSsDREBcDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that theSsDREBtranscription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression.

scholarly journals The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jiabin Shen ◽  
Bo Lv ◽  
Liqiong Luo ◽  
Jianmei He ◽  
Chanjuan Mao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Environmental Changes ◽  
Abiotic Stress Tolerance ◽  
Late Embryogenesis Abundant ◽  
High Salt ◽  
Marker Genes ◽  
Drought Conditions

Abstract Plants can perceive environmental changes and respond to external stressors. Here, we show that OsNAC2, a member of the NAC transcription factor family, was strongly induced by ABA and osmotic stressors such as drought and high salt. With reduced yields under drought conditions at the flowering stage, OsNAC2 overexpression lines had lower resistance to high salt and drought conditions. RNAi plants showed enhanced tolerance to high salinity and drought stress at both the vegetative and flowering stages. Furthermore, RNAi plants had improved yields after drought stress. A microarray assay indicated that many ABA-dependent stress-related genes were down-regulated in OsNAC2 overexpression lines. We further confirmed that OsNAC2 directly binds the promoters of LATE EMBRYOGENESIS ABUNDANT 3 (OsLEA3) and Stress-Activated Protein Kinases 1 (OsSAPK1), two marker genes in the abiotic stress and ABA response pathways, respectively. Our results suggest that in rice OsNAC2 regulates both abiotic stress responses and ABA-mediated responses, and acts at the junction between the ABA and abiotic stress pathways.

Genomic-Assisted Breeding in Finger Millet (Eleusine Coracana (L.) Gaertn.) for Abiotic Stress Tolerance

2021 ◽  
pp. 291-317
Author(s):  
T. P. Ajeesh Krishna ◽  
T. Maharajan ◽  
S. Ignacimuthu ◽  
S. Antony Ceasar
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Finger Millet ◽  
Abiotic Stress Tolerance ◽  
Eleusine Coracana

scholarly journals Over-expression of a NAC 67 transcription factor from finger millet (Eleusine coracana L.) confers tolerance against salinity and drought stress in rice

2016 ◽  
Vol 16 (S1) ◽  
Author(s):  
Hifzur Rahman ◽  
Valarmathi Ramanathan ◽  
Jagedeeshselvam Nallathambi ◽  
Sudhakar Duraialagaraja ◽  
Raveendran Muthurajan
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Finger Millet ◽  
Eleusine Coracana ◽  
Over Expression

scholarly journals Molecular characterization of two AP2/ERF transcription factor genes from Egyptian tomato cultivar (Edkawy)

2017 ◽  
Vol 4 (1) ◽  
pp. 12 ◽  
Author(s):  
Kareem Mosa ◽  
Eslam Heb El-din ◽  
Ahmed Ismail ◽  
Fawzy El- Feky ◽  
Ali El-Refy
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Molecular Characterization ◽  
Abiotic Stress Tolerance ◽  
Isolation And Identification ◽  
Tomato Cultivar ◽  
Erf Transcription Factor

The tomato is ranked first amongst vegetable crops in Egypt in relation to surface area and production. The Egyptian tomato cultivar Edkawy has shown abiotic stress tolerance characteristics. However, there is not much information about the molecular characterization of this cultivar. Furthermore, information regarding the identification of abiotic stress tolerance genes from the Edkawy tomato cultivar is lacking. Here, we investigated the ability of the Edkawy cultivar to tolerate drought stress. Two varieties were used as a control in this study; Peto86 (sensitive variety) and Strain B (tolerant variety). Edkawy, Peto86 and Strain B varieties were exposed to drought stress by reducing the water supply gradually. Interestingly, Edkawy demonstrated a remarkable tolerance phenotype to drought stress. Furthermore, we identified and isolated two members of the AP2/ERF transcription factor family from Edkawy which are associated with abiotic stress, particularly drought, i.e. ERF1 and ERF5. Protein prediction, validation and active site prediction of ERF1 and ERF5 were also determined. In addition to the domain obtained by the pfam online tool, the interaction between Edkawy ERFs proteins and other proteins in the Solanaceae family was obtained. Furthermore, subcellular localization was determined by the ngLOC and Plant-mPLoc online tools. Characterization of the Edkawy tomato cultivar and isolation and identification of such transcription factors will help in the engineering of tomato plants with abiotic stress tolerance.

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