scholarly journals Efektifitas Antibakteri Krim Ekstrak Etanol Biji Alpukat (persea americana mill.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 499-508
Author(s):  
Elok Afrinda Iskarimah ◽  
Urmatul Waznah ◽  
W Wirasti ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Statistical Tests ◽  
Chemical Compounds ◽  
Seed Extract ◽  
Persea Americana ◽  
Adhesion Test ◽  
Physical Tests ◽  
The One ◽  
One Way Anova ◽  
Avocado Seed

AbstractInfectious diseases that often occur are infections caused by the Staphylococus aureus bacteria, these bacteria are found on the skin, respiratory tract and digestive tract. The avocado plant (Persea americana Mill.) is a plant whose seeds contain several chemical compounds that can be used as antibacterial agents. The purpose of this study was to determine the antibacterial effectiveness of the avocado seed extract cream (Persea americana Mill.) Against the growth of Staphylococus aureus. Test the inhibition of bacteria using the well method. The extraction method uses the meseration method with 96% ethanol as solvent. The research results were analyzed using the One-Way ANOVA test. Avocado seed extract (Persea americana Mill.) can be formulated into cream preparations that meet several physical tests of the preparation which include organoleptic test, homogenity test, pH test, viscosity test, adhesion test and spreadability test. The formulation of avocado seed extract cream (Persea americana Mill.) in this study was able to inhibit the growth of Staphylococcus aureus bacteria with the largest zone of inhibition found at a concentration of 10% is 19.1 mm. At a concentration of 6% is 15.1 mm, at a concentration of 8% is 18.2 mm. With the results of one-way ANOVA statistical tests obtained sig 0,00 <0,05 which means that the diameter of the inhibition between formulas was significant.Keywords: Antibacterial, avocado seed extract, cream, Staphylococcus aureus. AbstrakPenyakit infeksi yang sering terjadi adalah infeksi yang disebabkan oleh bakteri Staphylococus aureus, bakteri ini terdapat pada kulit, saluran pernafasan, dan saluran pencernaan. Tumbuhan alpukat (Persea americana Mill.) merupakan salah satu tumbuhan yang bagian bijinya memiliki beberapa senyawa kimia yang dapat digunakan sebagai antibakteri. Tujuan dari penelitian ini yaitu untuk mengetahui efektivitas antibakteri dari sediaan krim ekstrak biji alpukat (Persea americana Mill.) terhadap pertumbuhan bakteri Staphylococus aureus. Uji daya hambat bakteri menggunakan metode sumuran. Metode ekstraksi meggunakan metode meserasi dengan pelarut etanol 96%. Hasil penelitian dianalisis dengan menggunakan uji One-Way ANOVA. Ekstrak biji alpukat (Persea americana Mill.) dapat diformulasikan ke dalam sediaan krim yang memenuhi beberapa uji fisik sediaan meliputi uji organoleptis, uji homogenitas, uji pH, uji viskositas, uji daya lekat dan uji daya sebar. Formulasi sediaan krim ekstrak biji alpukat (Persea americana Mill.) pada penelitian ini mampu menghambat pertumbuhan bakteri Staphylococcus aureus dengan zona hambat terbesar terdapat pada konsentrasi 10% sebesar 19,1 mm. Pada konstrasi 6% sebesar 15,1 mm, pada konsentrasi 8% sebesar 18,2 mm. Dengan hasil uji statistik one-way ANOVA diperoleh sig 0,00<0,05 yang berarti diameter hambat antar formula terdapat perbedaan yang signifikan.Kata kunci: Antibakteri, ekstrak biji alpukat, krim, Staphylococcus aureus.

scholarly journals Evaluation of the antimicrobial capacity of Hass avocado seed extract (Persea americana) for potential application in the meat industry

DYNA ◽  
2018 ◽  
Vol 85 (207) ◽  
pp. 346-350
Author(s):  
Laura Alejandra Fernández-Castañeda ◽  
Henry Arias-Candamil ◽  
Bryan Zapata-Torres ◽  
Maurem Paola Ardila-Castañeda
Keyword(s):  
Staphylococcus Aureus ◽  
Meat Products ◽  
Seed Extract ◽  
Persea Americana ◽  
Hot Water ◽  
Nitroso Compounds ◽  
Meat Industry ◽  
Microbiological Test ◽  
Hass Avocado ◽  
Avocado Seed

NaNO2 is used in meat products to inhibit pathogenic microorganisms; its use is limited, and it forms carcinogenic N-nitroso compounds. There is currently a great demand for natural products. The Hass avocado seed extract produces an antimicrobial reaction against bacteria such as Staphylococcus aureus. After the seed of the Hass avocado (AS) had been dehydrated at 50°C for 10 hours, we undertook a quality and analysis of the moisture and microbiological test. The extract was obtained in hot water and in solvents to perform an antimicrobial sensitivity test, which is an inhibition halo test using the strain Staphylococcus aureus as microorganisms. A minimum capacity inhibition test was also carried out. The concentration of the extract by solvents was 7 mg/mL, and it presented an inhibition halo of 1.8mm.The combination of AS and nitrites caused oxidation and darkening in the halos. The compounds that were extracted from the Hass avocado seeds with the methods used are not effective against S. aureus.

scholarly journals Developing New Health Material: The Utilization of Spray Drying Technology on Avocado (Persea Americana Mill.) Seed Powder

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 139 ◽  
Author(s):  
Karen Alissa ◽  
Yu-Chi Hung ◽  
Chih Yao Hou ◽  
GiGi Chin Wen Lim ◽  
Jhih-Ying Ciou
Keyword(s):  
Spray Drying ◽  
Water Activity ◽  
B Value ◽  
Seed Extract ◽  
Persea Americana ◽  
A Value ◽  
L Value ◽  
Drying Technology ◽  
Spray Dried ◽  
Avocado Seed

Avocado (Persea Americana Mill.) generates byproducts, especially the avocado seeds. Hence, the aim of this study was to investigate the potential utilization of avocado seed as a very important, high phenolic content, climacteric fruit with unique characteristics and high nutritional properties. As such, theantioxidative test is conducted, then spray drying is used to produce avocado seed powder. The objective of this study was to develop an avocado seed powder using the spray drying technique by investigating the solution stability with different avocado seed extract concentrations, and to determine the physical properties of spray dried avocado powder that consists of powder yield, moisture, water activity, solubility, and color. The avocado seed extract was mixed with maltodextrin and water and homogenized for 10 min at 8000 rpm. The avocado seed solution was then spray dried with different inlet temperatures and feed flow rates. The spray dried avocado seed powder was analyzed for its yield, moisture content, water activity, solubility, and color. It was reported that the solution with the least avocado extract concentration (10 g) had the best stability in terms of presence of solute particles and color. The avocado seed powder obtained from this experiment had yield ranges from 24.46–35.47%, moisture content ranges from 7.18–7.96%, water activity ranges from 0.27–0.34, solubility ranges from 55.50–79.67 seconds, L* value ranges from 38.38–41.05, a* value ranges from 6.20–7.25, and b* value ranges from 13.33–15.17. In addition, increasing inlet temperature resulted in an increase in powder yield, solubility, a* value, and b*value, as well as a decrease in moisture, water activity, and L* value. Meanwhile, increasing the feed flow rate resulted in an increase in powder yield, moisture, water activity, and all L*, a*, b* values, as well as a decrease in solubility. In conclusion, spray drying technology is able to develop avocado seed powder.

scholarly journals Uji efek ekstrak biji alpukat (Persea americana Mill.) terhadap kadar kolesterol total pada tikus wistar (Rattus norvegicus)

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Abraham T. Suhendra ◽  
Henoch Awaloei ◽  
Jane Wuisan
Keyword(s):  
Traditional Medicine ◽  
Wistar Rats ◽  
Seed Extract ◽  
Persea Americana ◽  
Blood Cholesterol ◽  
Control Group ◽  
Tropical Country ◽  
Total Blood ◽  
Total Blood Cholesterol ◽  
Avocado Seed

Abstract: Indonesia is a tropical country that has more than 1,000 kinds of medicinal plants. Medicinal plant is the use of part(s) of plants as ingredients in traditional medicine or synthetic drugs. One of the traditional medicine is avocado. Several studies have concluded that the avocado fruit and leaves can reduce total blood cholesterol levels while the seed is used in Nigeria for treating hypertension. This was an experimental study. Samples consisted of 12 male Wistar rats, divided into 4 groups (3 rats in each group). The treated groups were given propylthiouracil (PTU) for 14 days then the rats were given 125 mg/kg or 250 mg/kg of avocado seed extract for 14 days. The positive control group was given PTU 8 mg/rat for 14 days, followed by simvastatin 0.2 mg for 14 days. The negative control group was only given food and water. The results showed that the average level of total blood cholesterol in Wistar rats after treated with 125 mg/kg and 250 mg/kg avocado seed extract decreased by 18.1% and 31.2%, respectively. Conclusion: Avocado seed extract had an effect in reducing total blood cholesterol of Wistar rats.Keywords: avocado seed, total cholesterol levelAbstrak: Indonesia merupakan salah satu negara tropis yang memiliki banyak tumbuhan dimana lebih dari 1000 tumbuhan tersebut dapat digunakan sebagai tanaman obat. Tanaman obat ialah penggunaan bagian dari tanaman atau tumbuhan sebagai bahan dalam pembuatan obat tradisional maupun obat sintetik. Salah satu tanaman yang digunakan sebagai obat tradisional ialah alpukat. Beberapa penelitian mengenai tanaman alpukat menunjukkan bahwa buah dan daun dari tanaman Alpukat dapat menurunkan total kolesterol dalam darah, sedangkan biji alpukat digunakan di Nigeria sebagai pengobatan untuk hipertensi. Penelitian ini menggunakan metode eksperimental. Sampel ialah 12 ekor tikus Wistar jantan yang dibagi menjadi 4 kelompok (setiap kelompok terdiri dari 3 tikus). Kelompok-kelompok perlakuan diberikan propiltiourasil (PTU) selama 14 hari kemudian diberikan 125 mg/kg atau 250 mg/kg ekstrak biji Alpukat selama 14 hari. Kelompok kontrol positif diberikan PTU selama 14 hari kemudian diberikan simvastatin 0,20 mg/kg selama 14 hari. Kelompok kontrol negatif hanya diberikan pakan dan air. Hasil penelitian memperlihatkan rerata kadar kolesterol total tikus Wistar setelah diberikan 125 mg/kg dan 250 mg/kg ekstrak biji alpukat menurun sebesar 18,1% dan 31,2%. Simpulan: Ekstrak biji Alpukat berefek menurunkan kadar kolesterol total pada tikus Wistar.Kata kunci: biji alpukat, kadar kolesterol total

scholarly journals Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americanaMill., c.v.Hass)

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Eduardo Padilla-Camberos ◽  
Moisés Martínez-Velázquez ◽  
José Miguel Flores-Fernández ◽  
Socorro Villanueva-Rodríguez
Keyword(s):  
Acute Toxicity ◽  
Traditional Medicine ◽  
Micronucleus Test ◽  
Pharmaceutical Medicine ◽  
Micronucleus Assay ◽  
Negative Control ◽  
Seed Extract ◽  
Persea Americana ◽  
Genotoxic Activity ◽  
Avocado Seed

The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products.Persea americanaseeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract ofPersea americanawas evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle); therefore, it is considered that the avocado seed extract showed no genotoxic activity in the micronucleus test.

Pengaruh Pemberian Ekstrak Kulit Buah Alpukat (Persea Americana) Terhadap Kadar Malondialdehida (MDA) Jaringan Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Parasetamol Dosis Tinggi

2018 ◽  
Vol 15 (2) ◽  
pp. 177
Author(s):  
YUNITA SURYA PRATIWI ◽  
SULISTIANA PRABOWO
Keyword(s):  
Metabolic Pathways ◽  
Thiobarbituric Acid ◽  
Food Group ◽  
Persea Americana ◽  
Hepatic Tissue ◽  
High Dose ◽  
Male Wistar Rats ◽  
The One ◽  
One Way Anova ◽  
Peel Extract

<p>Administration of high-dose paracetamol may increase metabolic pathways that produce N-acetyl-p-benzoquinonimine (NAPQI). NAPQI is reactive substance, resulting in hepatic tissue damage and increase liver MDA level. Avocado peel extract contains flavonoids that act as antioxidants so it is expected may decrease liver MDA level of high-dose paracetamol administration.</p><p>This research used 24 male Wistar rats divided into 3 groups: group of rats fed with standard food, group of rats given paracetamol 1,750 mg/kg BW on day 8, and group of rats given avocado peel extract 1,400 mg/kg/day for 8 days and paracetamol 1750mg/kg BW on day 8. Liver MDA levels were checked on day 9 by thiobarbituric acid<em> </em>(TBA) method.</p><p>The One-Way ANOVA test showed that the liver MDA level of the group of rats given high dose of paracetamol (=312.38±47.830 nmol/g) was significantly higher (p = 0.001) compared to the liver MDA level of the group of rats fed with standard food (=184.50±57.021 nmol/g). The liver MDA level of group of rats given high-dose paracetamol and avocado peel extract<em> </em>(=268.50±91.834 nmol/g) did not significantly decrease (p =0.213) compared to the liver MDA level of group of rats given high dose of paracetamol (=312.38±47.830 nmol/g).</p><p>The conclusion of this research showed that high-dose paracetamol significantly increased liver MDA level and avocado peel extract tend to decrease liver MDA level because avocado peel consists flavonoid, hydroxynnamic acid<em>, </em>keratinoid, vitamin C, dan vitamin E that function as antioxidants.</p><p> </p><p><strong>Keywords</strong>: <em>Paracetamol, MDA, Persea americana</em></p>

scholarly journals Uji daya hambat ekstrak biji buah alpukat (Persea americana Mill.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Anggriana H. Bujung ◽  
Heriyannis Homenta ◽  
Johanna A. Khoman
Keyword(s):  
Streptococcus Mutans ◽  
Inhibitory Effect ◽  
Seed Extract ◽  
Persea Americana ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Paper Disk ◽  
Post Test ◽  
Avocado Seed

Abstract: In addition to daily consumption, avocado is also used to treat oral diseases. Scientific studies showed that avocado seed contained flavonoids, tannins, and alkaloid which were expected to inhibit bacterial growth. This study was aimed to obtain the bacterial inhibitory effect of avocado seed (Persea americana Mill.) extract on Streptococcus mutans as the primary cause of dental caries. This was a true experimental study with the post test only control group design. This study used a modified Kirby-Bauer method with paper disk. The positive control was erythromycin and the negative control was aquadest. Avocado seeds were extracted by using maceration method with 96% ethanol. The Streptococcus mutans bacteria were obtained from pure stock of Microbiology Laboratory of Pharmacy Study Program University of Sam Ratulangi Manado. The results showed that the mean diameter of inhibition zone of avocado seed extract was 21.8 mm which was classified as very strong inhibition. Conclusion: Persea Americana Mill. seed extract had a very strong inhibitory effect on the growth of Streptococcus mutans.Keywords: avocado seed (Persea americana Mill.), Streptococcus mutans, inhibition effectAbstrak: Selain menjadi bahan konsumsi masyarakat yang lezat, ternyata alpukat telah lama dipercaya dapat mengobati penyakit di dalam rongga mulut. Di dalam buah alpukat terdapat biji yang terbukti melalui penelitian ilmiah mengandung flavonoid, alkaloid, dan tannin yang mampu menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji buah alpukat (Persea americana Mill.) terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan ialah modifikasi Kirby-Bauer dengan paper disk. Kontrol positif menggunakan antibiotik eritromisin dan kontrol negatif menggunakan akuades. Biji buah alpukat diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil penelitian menunjukkan diameter rerata zona hambat dari ekstrak biji buah alpukat yang terbentuk ialah 21,8 mm dan digolongkan sebagai zona hambat sangat kuat. Simpulan: Ekstrak biji buah alpukat memiliki daya hambat sangat kuat terhadap pertumbuhan Streptococcus mutans.Kata kunci: biji buah alpukat (Persea americana Mill.), Streptococcus mutans, daya hambat

scholarly journals Pemberian Ekstrak Biji Alpukat (Persea americana Mill.) dan Biji Sirsak (Annona muricata L.) terhadap Mortalitas Larva Nyamuk Culex Sp

2017 ◽  
Vol 10 (02) ◽  
pp. 1-15
Author(s):  
S. Nombe ◽  
Diah Karunia Binawati
Keyword(s):  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Persea Americana ◽  
Natural Material ◽  
Mosquito Larvae ◽  
Annona Muricata ◽  
Randomized Design ◽  
And Control ◽  
Culex Sp ◽  
Avocado Seed

Vegetable insecticide is a natural material that is environmentally friendly. This material can be used in the prevention and control of mosquito larvae, especially Culex sp. Culex sp is the main vector of filariasis disease and dengue hemorrhagic fever (DHF). Use of natural materials is very good and environment friendly as insectcide compared with chemicals. Avocado seed extract (Persea americana Mill) is known to have secondary metabolite content of alkaloids, saponins, triterpenoids while soursop extract (Annona muricata L) in the form of acetogenin, squamocin and annonacin. This research needs to use of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) as larvacide of Culex sp. The aim of this research is to know the effect of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) to mortality of Culex sp Instar III mosquito larvae and LC50 for 24 hours. This study was an experimental study using a complete randomized design (RAL) with five treatment concentrations (0, 2, 4, 6, and 8%) avocado seed extract (P. americana Mill) and soursop extracts extract (A. muricata L) as much as five repetitions. The data obtained were analyzed by using One Way ANOVA to show the effect on the treatment. The results showed that giving of avocado seed extract (P. americana Mill) with 8% concentration (23,8 ± 0,45 larvae) was optimal in killing Culex sp mosquito larvae. While giving soursop seed extract (A. muricata L) with 4% concentration (25 ± 0,0 larva) optimal in killing Culex sp mosquito larvae. Avocado seed extract (P. americana Mill) has an effect as a larvacide against Culex sp. instar III with LC50 at concentration 2,486% while soursop extract (A. muricata L) with LC50 value at concentration 1,605%.   Keywords: Larvacide, soursop seed extract, avocado seed extract, Culex sp

scholarly journals AVOCADO SEEDS (Persea americana Mill.): FORMULATIONS OF ELIXIR VARIED BY SOLVENTS COMPOSITION

2020 ◽  
Vol 3 (1) ◽  
pp. 01-03
Author(s):  
Septia Andini ◽  
Lusi Indriani ◽  
Erni Rustiani
Keyword(s):  
Propylene Glycol ◽  
Kidney Stones ◽  
Health Problem ◽  
Specific Weight ◽  
Seed Extract ◽  
Persea Americana ◽  
Total Flavonoids ◽  
Test Results ◽  
The World ◽  
Avocado Seed

Kidney stones disease is asignificant health problem in the world and Indonesia is no exception. Traditionally, avocado seeds can be used as a laxative medicine for kidney stones disease. Elixiris a clear and sweet hidroalkohol solution intended for oral use and a flavor is usually added to enhance the taste. This study was to determine theformula of the avocado seed elixir which has the best quality and the level of flavonoids.Elixir testing was performedusing several methods such as organoleptic, limpidity, viscosity, pH and specific weightas well as total flavonoids. The result showed that the best elixir formula was the Formula I which composed of 7%avocado seed extract, 5% ethanol, 10% propylene glycol, 40% sirupus simplex, 0.1%, essence mint and 100%aquadestilata ad. Furthermore, Elixirhad a brown color, mint aroma and pretty sweettaste. The test results showed that elixir had a viscosity of 7.92 cP; pH of 5.65; specific weight of 1.0413 g/mL andtotal flavonoids of 2.1020%.

scholarly journals ANTIBACTERIAL ACTIVITY TESTING OF HAND SANITIZER GEL EXTRACT OF CORIANDER (CORIANDRUM SATIVUM L.) SEEDS AGAINST STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 3 (2) ◽  
pp. 98
Author(s):  
Kadek Sutri Ariyanthini ◽  
Elisabeth Angelina ◽  
Kadek Nanda Banyu Permana ◽  
Fiorenza Jocelyn Thelmalina ◽  
I Gusti Ngurah Jemmy Anton Prasetia
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Habitat ◽  
Skin Surface ◽  
Seed Extract ◽  
Diffusion Method ◽  
Hand Sanitizer ◽  
Inhibition Zones ◽  
The One ◽  
Coriander Seed

Background: Staphylococcus aureus is a bacterium that causes bacteremia in COVID-19 pneumonitis patients. S. aureus is responsible for 80% of suppurative disease, with the skin surface as its natural habitat. So, maintaining hand hygiene during a pandemic is very important. Currently, people prefer the use of practical hand sanitizers. Most hand sanitizers contain alcohol, which has the potential to irritate. Thus, the active substance of coriander seed extract is used as a substitute for alcohol. Objective: To determine the characteristics of the gel and the concentration of coriander seed extract which gave antibacterial effect against S. aureus. Methods: The resulting hand sanitizer gel was then tested for characteristics and antibacterial activity against Staphylococcus aureus by the Kirby-Bauer diffusion method and analyzed by the One Way Anova-LSD method. Result: The results showed that preparations with concentrations of F1(2%), F2(4%), and F3(6%) respectively had inhibition zones of 1.00 ± 0.82 mm, 5.00 ± 0.41 mm, and 5 .25 ± 1.26 mm. Conclusion: Formulas 2 and 3 were able to inhibit the growth of S. aureus with moderate inhibition category. The hand sanitizer gel meets the requirements for organoleptic tests, pH, homogeneity, dispersibility, and adhesion. However, the viscosity of the preparation did not meet the requirements. Keywords: Coriander Seed Extract, Gel Hand Sanitizer, Antibacterial

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